Job ID = 6455467 SRX = SRX287812 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T10:12:21 prefetch.2.10.7: 1) Downloading 'SRR870001'... 2020-06-21T10:12:21 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T10:16:19 prefetch.2.10.7: HTTPS download succeed 2020-06-21T10:16:19 prefetch.2.10.7: 1) 'SRR870001' was downloaded successfully Read 17745143 spots for SRR870001/SRR870001.sra Written 17745143 spots for SRR870001/SRR870001.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:16 17745143 reads; of these: 17745143 (100.00%) were unpaired; of these: 877789 (4.95%) aligned 0 times 14450234 (81.43%) aligned exactly 1 time 2417120 (13.62%) aligned >1 times 95.05% overall alignment rate Time searching: 00:04:16 Overall time: 00:04:16 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1810107 / 16867354 = 0.1073 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:26:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX287812/SRX287812.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX287812/SRX287812.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX287812/SRX287812.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX287812/SRX287812.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:26:02: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:26:02: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:26:07: 1000000 INFO @ Sun, 21 Jun 2020 19:26:12: 2000000 INFO @ Sun, 21 Jun 2020 19:26:17: 3000000 INFO @ Sun, 21 Jun 2020 19:26:22: 4000000 INFO @ Sun, 21 Jun 2020 19:26:26: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:26:31: 6000000 INFO @ Sun, 21 Jun 2020 19:26:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX287812/SRX287812.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX287812/SRX287812.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX287812/SRX287812.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX287812/SRX287812.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:26:32: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:26:32: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:26:36: 7000000 INFO @ Sun, 21 Jun 2020 19:26:37: 1000000 INFO @ Sun, 21 Jun 2020 19:26:41: 8000000 INFO @ Sun, 21 Jun 2020 19:26:42: 2000000 INFO @ Sun, 21 Jun 2020 19:26:46: 9000000 INFO @ Sun, 21 Jun 2020 19:26:47: 3000000 INFO @ Sun, 21 Jun 2020 19:26:51: 10000000 INFO @ Sun, 21 Jun 2020 19:26:52: 4000000 INFO @ Sun, 21 Jun 2020 19:26:56: 11000000 INFO @ Sun, 21 Jun 2020 19:26:57: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:27:01: 12000000 INFO @ Sun, 21 Jun 2020 19:27:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX287812/SRX287812.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX287812/SRX287812.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX287812/SRX287812.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX287812/SRX287812.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:27:02: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:27:02: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:27:02: 6000000 INFO @ Sun, 21 Jun 2020 19:27:07: 13000000 INFO @ Sun, 21 Jun 2020 19:27:08: 7000000 INFO @ Sun, 21 Jun 2020 19:27:08: 1000000 INFO @ Sun, 21 Jun 2020 19:27:12: 14000000 INFO @ Sun, 21 Jun 2020 19:27:13: 8000000 INFO @ Sun, 21 Jun 2020 19:27:13: 2000000 INFO @ Sun, 21 Jun 2020 19:27:18: 15000000 INFO @ Sun, 21 Jun 2020 19:27:18: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 19:27:18: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 19:27:18: #1 total tags in treatment: 15057247 INFO @ Sun, 21 Jun 2020 19:27:18: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:27:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:27:19: 9000000 INFO @ Sun, 21 Jun 2020 19:27:19: 3000000 INFO @ Sun, 21 Jun 2020 19:27:19: #1 tags after filtering in treatment: 15057240 INFO @ Sun, 21 Jun 2020 19:27:19: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:27:19: #1 finished! INFO @ Sun, 21 Jun 2020 19:27:19: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:27:19: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:27:20: #2 number of paired peaks: 85 WARNING @ Sun, 21 Jun 2020 19:27:20: Too few paired peaks (85) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:27:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX287812/SRX287812.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287812/SRX287812.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287812/SRX287812.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287812/SRX287812.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 19:27:24: 10000000 INFO @ Sun, 21 Jun 2020 19:27:24: 4000000 INFO @ Sun, 21 Jun 2020 19:27:29: 11000000 INFO @ Sun, 21 Jun 2020 19:27:29: 5000000 INFO @ Sun, 21 Jun 2020 19:27:35: 12000000 INFO @ Sun, 21 Jun 2020 19:27:35: 6000000 INFO @ Sun, 21 Jun 2020 19:27:40: 13000000 INFO @ Sun, 21 Jun 2020 19:27:40: 7000000 INFO @ Sun, 21 Jun 2020 19:27:46: 14000000 INFO @ Sun, 21 Jun 2020 19:27:46: 8000000 INFO @ Sun, 21 Jun 2020 19:27:51: 15000000 INFO @ Sun, 21 Jun 2020 19:27:51: 9000000 INFO @ Sun, 21 Jun 2020 19:27:52: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 19:27:52: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 19:27:52: #1 total tags in treatment: 15057247 INFO @ Sun, 21 Jun 2020 19:27:52: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:27:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:27:52: #1 tags after filtering in treatment: 15057240 INFO @ Sun, 21 Jun 2020 19:27:52: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:27:52: #1 finished! INFO @ Sun, 21 Jun 2020 19:27:52: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:27:52: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:27:53: #2 number of paired peaks: 85 WARNING @ Sun, 21 Jun 2020 19:27:53: Too few paired peaks (85) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:27:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX287812/SRX287812.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287812/SRX287812.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287812/SRX287812.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287812/SRX287812.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 19:27:57: 10000000 INFO @ Sun, 21 Jun 2020 19:28:02: 11000000 INFO @ Sun, 21 Jun 2020 19:28:07: 12000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 19:28:13: 13000000 INFO @ Sun, 21 Jun 2020 19:28:18: 14000000 INFO @ Sun, 21 Jun 2020 19:28:24: 15000000 INFO @ Sun, 21 Jun 2020 19:28:24: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 19:28:24: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 19:28:24: #1 total tags in treatment: 15057247 INFO @ Sun, 21 Jun 2020 19:28:24: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:28:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:28:25: #1 tags after filtering in treatment: 15057240 INFO @ Sun, 21 Jun 2020 19:28:25: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:28:25: #1 finished! INFO @ Sun, 21 Jun 2020 19:28:25: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:28:25: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:28:26: #2 number of paired peaks: 85 WARNING @ Sun, 21 Jun 2020 19:28:26: Too few paired peaks (85) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:28:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX287812/SRX287812.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287812/SRX287812.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287812/SRX287812.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287812/SRX287812.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。