Job ID = 6455466 SRX = SRX287811 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T09:59:55 prefetch.2.10.7: 1) Downloading 'SRR870000'... 2020-06-21T09:59:55 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T10:02:18 prefetch.2.10.7: HTTPS download succeed 2020-06-21T10:02:19 prefetch.2.10.7: 'SRR870000' is valid 2020-06-21T10:02:19 prefetch.2.10.7: 1) 'SRR870000' was downloaded successfully Read 14938970 spots for SRR870000/SRR870000.sra Written 14938970 spots for SRR870000/SRR870000.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:49 14938970 reads; of these: 14938970 (100.00%) were unpaired; of these: 799405 (5.35%) aligned 0 times 12317009 (82.45%) aligned exactly 1 time 1822556 (12.20%) aligned >1 times 94.65% overall alignment rate Time searching: 00:03:49 Overall time: 00:03:49 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1341013 / 14139565 = 0.0948 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:10:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX287811/SRX287811.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX287811/SRX287811.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX287811/SRX287811.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX287811/SRX287811.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:10:51: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:10:51: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:10:57: 1000000 INFO @ Sun, 21 Jun 2020 19:11:03: 2000000 INFO @ Sun, 21 Jun 2020 19:11:10: 3000000 INFO @ Sun, 21 Jun 2020 19:11:16: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:11:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX287811/SRX287811.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX287811/SRX287811.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX287811/SRX287811.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX287811/SRX287811.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:11:21: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:11:21: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:11:23: 5000000 INFO @ Sun, 21 Jun 2020 19:11:28: 1000000 INFO @ Sun, 21 Jun 2020 19:11:30: 6000000 INFO @ Sun, 21 Jun 2020 19:11:35: 2000000 INFO @ Sun, 21 Jun 2020 19:11:37: 7000000 INFO @ Sun, 21 Jun 2020 19:11:42: 3000000 INFO @ Sun, 21 Jun 2020 19:11:44: 8000000 INFO @ Sun, 21 Jun 2020 19:11:49: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:11:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX287811/SRX287811.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX287811/SRX287811.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX287811/SRX287811.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX287811/SRX287811.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:11:51: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:11:51: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:11:51: 9000000 INFO @ Sun, 21 Jun 2020 19:11:56: 5000000 INFO @ Sun, 21 Jun 2020 19:11:58: 10000000 INFO @ Sun, 21 Jun 2020 19:11:58: 1000000 INFO @ Sun, 21 Jun 2020 19:12:03: 6000000 INFO @ Sun, 21 Jun 2020 19:12:05: 2000000 INFO @ Sun, 21 Jun 2020 19:12:06: 11000000 INFO @ Sun, 21 Jun 2020 19:12:10: 7000000 INFO @ Sun, 21 Jun 2020 19:12:13: 3000000 INFO @ Sun, 21 Jun 2020 19:12:13: 12000000 INFO @ Sun, 21 Jun 2020 19:12:17: 8000000 INFO @ Sun, 21 Jun 2020 19:12:19: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 19:12:19: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 19:12:19: #1 total tags in treatment: 12798552 INFO @ Sun, 21 Jun 2020 19:12:19: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:12:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:12:20: #1 tags after filtering in treatment: 12798536 INFO @ Sun, 21 Jun 2020 19:12:20: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:12:20: #1 finished! INFO @ Sun, 21 Jun 2020 19:12:20: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:12:20: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:12:20: 4000000 INFO @ Sun, 21 Jun 2020 19:12:20: #2 number of paired peaks: 97 WARNING @ Sun, 21 Jun 2020 19:12:20: Too few paired peaks (97) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:12:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX287811/SRX287811.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287811/SRX287811.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287811/SRX287811.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287811/SRX287811.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 19:12:24: 9000000 INFO @ Sun, 21 Jun 2020 19:12:27: 5000000 INFO @ Sun, 21 Jun 2020 19:12:31: 10000000 INFO @ Sun, 21 Jun 2020 19:12:34: 6000000 INFO @ Sun, 21 Jun 2020 19:12:39: 11000000 INFO @ Sun, 21 Jun 2020 19:12:41: 7000000 INFO @ Sun, 21 Jun 2020 19:12:46: 12000000 INFO @ Sun, 21 Jun 2020 19:12:48: 8000000 INFO @ Sun, 21 Jun 2020 19:12:52: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 19:12:52: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 19:12:52: #1 total tags in treatment: 12798552 INFO @ Sun, 21 Jun 2020 19:12:52: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:12:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:12:52: #1 tags after filtering in treatment: 12798536 INFO @ Sun, 21 Jun 2020 19:12:52: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:12:52: #1 finished! INFO @ Sun, 21 Jun 2020 19:12:52: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:12:52: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:12:53: #2 number of paired peaks: 97 WARNING @ Sun, 21 Jun 2020 19:12:53: Too few paired peaks (97) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:12:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX287811/SRX287811.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287811/SRX287811.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287811/SRX287811.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287811/SRX287811.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 19:12:55: 9000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 19:13:01: 10000000 INFO @ Sun, 21 Jun 2020 19:13:08: 11000000 INFO @ Sun, 21 Jun 2020 19:13:14: 12000000 INFO @ Sun, 21 Jun 2020 19:13:20: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 19:13:20: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 19:13:20: #1 total tags in treatment: 12798552 INFO @ Sun, 21 Jun 2020 19:13:20: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:13:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:13:20: #1 tags after filtering in treatment: 12798536 INFO @ Sun, 21 Jun 2020 19:13:20: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:13:20: #1 finished! INFO @ Sun, 21 Jun 2020 19:13:20: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:13:20: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:13:21: #2 number of paired peaks: 97 WARNING @ Sun, 21 Jun 2020 19:13:21: Too few paired peaks (97) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:13:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX287811/SRX287811.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287811/SRX287811.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287811/SRX287811.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287811/SRX287811.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。