Job ID = 6455441 SRX = SRX287792 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T09:59:10 prefetch.2.10.7: 1) Downloading 'SRR869981'... 2020-06-21T09:59:10 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T10:01:09 prefetch.2.10.7: HTTPS download succeed 2020-06-21T10:01:09 prefetch.2.10.7: 1) 'SRR869981' was downloaded successfully Read 16527336 spots for SRR869981/SRR869981.sra Written 16527336 spots for SRR869981/SRR869981.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:49 16527336 reads; of these: 16527336 (100.00%) were unpaired; of these: 915862 (5.54%) aligned 0 times 13572588 (82.12%) aligned exactly 1 time 2038886 (12.34%) aligned >1 times 94.46% overall alignment rate Time searching: 00:03:49 Overall time: 00:03:49 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 846678 / 15611474 = 0.0542 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:10:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX287792/SRX287792.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX287792/SRX287792.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX287792/SRX287792.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX287792/SRX287792.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:10:15: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:10:15: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:10:22: 1000000 INFO @ Sun, 21 Jun 2020 19:10:30: 2000000 INFO @ Sun, 21 Jun 2020 19:10:36: 3000000 INFO @ Sun, 21 Jun 2020 19:10:43: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:10:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX287792/SRX287792.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX287792/SRX287792.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX287792/SRX287792.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX287792/SRX287792.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:10:45: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:10:45: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:10:51: 5000000 INFO @ Sun, 21 Jun 2020 19:10:52: 1000000 INFO @ Sun, 21 Jun 2020 19:10:58: 6000000 INFO @ Sun, 21 Jun 2020 19:10:59: 2000000 INFO @ Sun, 21 Jun 2020 19:11:05: 7000000 INFO @ Sun, 21 Jun 2020 19:11:06: 3000000 INFO @ Sun, 21 Jun 2020 19:11:12: 8000000 INFO @ Sun, 21 Jun 2020 19:11:13: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:11:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX287792/SRX287792.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX287792/SRX287792.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX287792/SRX287792.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX287792/SRX287792.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:11:15: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:11:15: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:11:19: 9000000 INFO @ Sun, 21 Jun 2020 19:11:20: 5000000 INFO @ Sun, 21 Jun 2020 19:11:22: 1000000 INFO @ Sun, 21 Jun 2020 19:11:26: 10000000 INFO @ Sun, 21 Jun 2020 19:11:27: 6000000 INFO @ Sun, 21 Jun 2020 19:11:29: 2000000 INFO @ Sun, 21 Jun 2020 19:11:33: 11000000 INFO @ Sun, 21 Jun 2020 19:11:34: 7000000 INFO @ Sun, 21 Jun 2020 19:11:36: 3000000 INFO @ Sun, 21 Jun 2020 19:11:40: 12000000 INFO @ Sun, 21 Jun 2020 19:11:41: 8000000 INFO @ Sun, 21 Jun 2020 19:11:43: 4000000 INFO @ Sun, 21 Jun 2020 19:11:47: 13000000 INFO @ Sun, 21 Jun 2020 19:11:48: 9000000 INFO @ Sun, 21 Jun 2020 19:11:51: 5000000 INFO @ Sun, 21 Jun 2020 19:11:54: 14000000 INFO @ Sun, 21 Jun 2020 19:11:55: 10000000 INFO @ Sun, 21 Jun 2020 19:11:58: 6000000 INFO @ Sun, 21 Jun 2020 19:12:00: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 19:12:00: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 19:12:00: #1 total tags in treatment: 14764796 INFO @ Sun, 21 Jun 2020 19:12:00: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:12:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:12:01: #1 tags after filtering in treatment: 14764784 INFO @ Sun, 21 Jun 2020 19:12:01: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:12:01: #1 finished! INFO @ Sun, 21 Jun 2020 19:12:01: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:12:01: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:12:02: #2 number of paired peaks: 26 WARNING @ Sun, 21 Jun 2020 19:12:02: Too few paired peaks (26) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:12:02: Process for pairing-model is terminated! INFO @ Sun, 21 Jun 2020 19:12:02: 11000000 cut: /home/okishinya/chipatlas/results/dm6/SRX287792/SRX287792.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287792/SRX287792.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287792/SRX287792.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287792/SRX287792.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 19:12:05: 7000000 INFO @ Sun, 21 Jun 2020 19:12:09: 12000000 INFO @ Sun, 21 Jun 2020 19:12:12: 8000000 INFO @ Sun, 21 Jun 2020 19:12:17: 13000000 INFO @ Sun, 21 Jun 2020 19:12:19: 9000000 INFO @ Sun, 21 Jun 2020 19:12:24: 14000000 INFO @ Sun, 21 Jun 2020 19:12:26: 10000000 INFO @ Sun, 21 Jun 2020 19:12:30: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 19:12:30: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 19:12:30: #1 total tags in treatment: 14764796 INFO @ Sun, 21 Jun 2020 19:12:30: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:12:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:12:30: #1 tags after filtering in treatment: 14764784 INFO @ Sun, 21 Jun 2020 19:12:30: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:12:30: #1 finished! INFO @ Sun, 21 Jun 2020 19:12:30: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:12:30: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:12:31: #2 number of paired peaks: 26 WARNING @ Sun, 21 Jun 2020 19:12:31: Too few paired peaks (26) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:12:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX287792/SRX287792.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287792/SRX287792.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287792/SRX287792.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287792/SRX287792.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 19:12:33: 11000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 19:12:39: 12000000 INFO @ Sun, 21 Jun 2020 19:12:46: 13000000 INFO @ Sun, 21 Jun 2020 19:12:52: 14000000 INFO @ Sun, 21 Jun 2020 19:12:57: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 19:12:57: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 19:12:57: #1 total tags in treatment: 14764796 INFO @ Sun, 21 Jun 2020 19:12:57: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:12:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:12:58: #1 tags after filtering in treatment: 14764784 INFO @ Sun, 21 Jun 2020 19:12:58: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:12:58: #1 finished! INFO @ Sun, 21 Jun 2020 19:12:58: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:12:58: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:12:59: #2 number of paired peaks: 26 WARNING @ Sun, 21 Jun 2020 19:12:59: Too few paired peaks (26) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:12:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX287792/SRX287792.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287792/SRX287792.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287792/SRX287792.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287792/SRX287792.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。