Job ID = 6455440 SRX = SRX287791 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T10:09:36 prefetch.2.10.7: 1) Downloading 'SRR869980'... 2020-06-21T10:09:36 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T10:12:17 prefetch.2.10.7: HTTPS download succeed 2020-06-21T10:12:18 prefetch.2.10.7: 'SRR869980' is valid 2020-06-21T10:12:18 prefetch.2.10.7: 1) 'SRR869980' was downloaded successfully Read 15156232 spots for SRR869980/SRR869980.sra Written 15156232 spots for SRR869980/SRR869980.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:18 15156232 reads; of these: 15156232 (100.00%) were unpaired; of these: 814219 (5.37%) aligned 0 times 12230546 (80.70%) aligned exactly 1 time 2111467 (13.93%) aligned >1 times 94.63% overall alignment rate Time searching: 00:03:18 Overall time: 00:03:18 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 740839 / 14342013 = 0.0517 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:20:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX287791/SRX287791.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX287791/SRX287791.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX287791/SRX287791.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX287791/SRX287791.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:20:29: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:20:29: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:20:34: 1000000 INFO @ Sun, 21 Jun 2020 19:20:40: 2000000 INFO @ Sun, 21 Jun 2020 19:20:45: 3000000 INFO @ Sun, 21 Jun 2020 19:20:50: 4000000 INFO @ Sun, 21 Jun 2020 19:20:55: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:20:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX287791/SRX287791.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX287791/SRX287791.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX287791/SRX287791.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX287791/SRX287791.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:20:59: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:20:59: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:21:01: 6000000 INFO @ Sun, 21 Jun 2020 19:21:04: 1000000 INFO @ Sun, 21 Jun 2020 19:21:06: 7000000 INFO @ Sun, 21 Jun 2020 19:21:08: 2000000 INFO @ Sun, 21 Jun 2020 19:21:12: 8000000 INFO @ Sun, 21 Jun 2020 19:21:13: 3000000 INFO @ Sun, 21 Jun 2020 19:21:17: 9000000 INFO @ Sun, 21 Jun 2020 19:21:18: 4000000 INFO @ Sun, 21 Jun 2020 19:21:23: 10000000 INFO @ Sun, 21 Jun 2020 19:21:23: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:21:28: 6000000 INFO @ Sun, 21 Jun 2020 19:21:28: 11000000 INFO @ Sun, 21 Jun 2020 19:21:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX287791/SRX287791.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX287791/SRX287791.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX287791/SRX287791.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX287791/SRX287791.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:21:29: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:21:29: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:21:32: 7000000 INFO @ Sun, 21 Jun 2020 19:21:34: 1000000 INFO @ Sun, 21 Jun 2020 19:21:34: 12000000 INFO @ Sun, 21 Jun 2020 19:21:37: 8000000 INFO @ Sun, 21 Jun 2020 19:21:38: 2000000 INFO @ Sun, 21 Jun 2020 19:21:39: 13000000 INFO @ Sun, 21 Jun 2020 19:21:42: 9000000 INFO @ Sun, 21 Jun 2020 19:21:43: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 19:21:43: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 19:21:43: #1 total tags in treatment: 13601174 INFO @ Sun, 21 Jun 2020 19:21:43: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:21:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:21:43: 3000000 INFO @ Sun, 21 Jun 2020 19:21:43: #1 tags after filtering in treatment: 13601165 INFO @ Sun, 21 Jun 2020 19:21:43: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:21:43: #1 finished! INFO @ Sun, 21 Jun 2020 19:21:43: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:21:43: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:21:44: #2 number of paired peaks: 51 WARNING @ Sun, 21 Jun 2020 19:21:44: Too few paired peaks (51) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:21:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX287791/SRX287791.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287791/SRX287791.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287791/SRX287791.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287791/SRX287791.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 19:21:47: 10000000 INFO @ Sun, 21 Jun 2020 19:21:48: 4000000 INFO @ Sun, 21 Jun 2020 19:21:52: 11000000 INFO @ Sun, 21 Jun 2020 19:21:53: 5000000 INFO @ Sun, 21 Jun 2020 19:21:57: 12000000 INFO @ Sun, 21 Jun 2020 19:21:58: 6000000 INFO @ Sun, 21 Jun 2020 19:22:02: 13000000 INFO @ Sun, 21 Jun 2020 19:22:02: 7000000 INFO @ Sun, 21 Jun 2020 19:22:05: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 19:22:05: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 19:22:05: #1 total tags in treatment: 13601174 INFO @ Sun, 21 Jun 2020 19:22:05: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:22:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:22:05: #1 tags after filtering in treatment: 13601165 INFO @ Sun, 21 Jun 2020 19:22:05: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:22:05: #1 finished! INFO @ Sun, 21 Jun 2020 19:22:05: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:22:05: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:22:06: #2 number of paired peaks: 51 WARNING @ Sun, 21 Jun 2020 19:22:06: Too few paired peaks (51) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:22:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX287791/SRX287791.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287791/SRX287791.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287791/SRX287791.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287791/SRX287791.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 19:22:07: 8000000 INFO @ Sun, 21 Jun 2020 19:22:12: 9000000 INFO @ Sun, 21 Jun 2020 19:22:17: 10000000 INFO @ Sun, 21 Jun 2020 19:22:21: 11000000 INFO @ Sun, 21 Jun 2020 19:22:27: 12000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 19:22:31: 13000000 INFO @ Sun, 21 Jun 2020 19:22:35: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 19:22:35: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 19:22:35: #1 total tags in treatment: 13601174 INFO @ Sun, 21 Jun 2020 19:22:35: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:22:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:22:35: #1 tags after filtering in treatment: 13601165 INFO @ Sun, 21 Jun 2020 19:22:35: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:22:35: #1 finished! INFO @ Sun, 21 Jun 2020 19:22:35: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:22:35: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:22:36: #2 number of paired peaks: 51 WARNING @ Sun, 21 Jun 2020 19:22:36: Too few paired peaks (51) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:22:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX287791/SRX287791.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287791/SRX287791.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287791/SRX287791.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287791/SRX287791.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。