Job ID = 6455375 SRX = SRX287736 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T09:59:55 prefetch.2.10.7: 1) Downloading 'SRR869925'... 2020-06-21T09:59:55 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T10:02:22 prefetch.2.10.7: HTTPS download succeed 2020-06-21T10:02:22 prefetch.2.10.7: 1) 'SRR869925' was downloaded successfully Read 17631207 spots for SRR869925/SRR869925.sra Written 17631207 spots for SRR869925/SRR869925.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:03:59 17631207 reads; of these: 17631207 (100.00%) were unpaired; of these: 992266 (5.63%) aligned 0 times 14904333 (84.53%) aligned exactly 1 time 1734608 (9.84%) aligned >1 times 94.37% overall alignment rate Time searching: 00:04:00 Overall time: 00:04:00 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1572827 / 16638941 = 0.0945 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:11:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX287736/SRX287736.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX287736/SRX287736.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX287736/SRX287736.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX287736/SRX287736.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:11:38: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:11:38: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:11:45: 1000000 INFO @ Sun, 21 Jun 2020 19:11:52: 2000000 INFO @ Sun, 21 Jun 2020 19:11:59: 3000000 INFO @ Sun, 21 Jun 2020 19:12:06: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:12:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX287736/SRX287736.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX287736/SRX287736.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX287736/SRX287736.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX287736/SRX287736.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:12:08: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:12:08: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:12:13: 5000000 INFO @ Sun, 21 Jun 2020 19:12:15: 1000000 INFO @ Sun, 21 Jun 2020 19:12:21: 6000000 INFO @ Sun, 21 Jun 2020 19:12:23: 2000000 INFO @ Sun, 21 Jun 2020 19:12:28: 7000000 INFO @ Sun, 21 Jun 2020 19:12:31: 3000000 INFO @ Sun, 21 Jun 2020 19:12:36: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:12:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX287736/SRX287736.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX287736/SRX287736.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX287736/SRX287736.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX287736/SRX287736.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:12:38: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:12:38: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:12:38: 4000000 INFO @ Sun, 21 Jun 2020 19:12:43: 9000000 INFO @ Sun, 21 Jun 2020 19:12:45: 1000000 INFO @ Sun, 21 Jun 2020 19:12:46: 5000000 INFO @ Sun, 21 Jun 2020 19:12:51: 10000000 INFO @ Sun, 21 Jun 2020 19:12:51: 2000000 INFO @ Sun, 21 Jun 2020 19:12:54: 6000000 INFO @ Sun, 21 Jun 2020 19:12:58: 3000000 INFO @ Sun, 21 Jun 2020 19:12:58: 11000000 INFO @ Sun, 21 Jun 2020 19:13:02: 7000000 INFO @ Sun, 21 Jun 2020 19:13:05: 4000000 INFO @ Sun, 21 Jun 2020 19:13:06: 12000000 INFO @ Sun, 21 Jun 2020 19:13:10: 8000000 INFO @ Sun, 21 Jun 2020 19:13:12: 5000000 INFO @ Sun, 21 Jun 2020 19:13:14: 13000000 INFO @ Sun, 21 Jun 2020 19:13:18: 9000000 INFO @ Sun, 21 Jun 2020 19:13:19: 6000000 INFO @ Sun, 21 Jun 2020 19:13:22: 14000000 INFO @ Sun, 21 Jun 2020 19:13:25: 10000000 INFO @ Sun, 21 Jun 2020 19:13:26: 7000000 INFO @ Sun, 21 Jun 2020 19:13:30: 15000000 INFO @ Sun, 21 Jun 2020 19:13:30: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 19:13:30: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 19:13:30: #1 total tags in treatment: 15066114 INFO @ Sun, 21 Jun 2020 19:13:30: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:13:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:13:31: #1 tags after filtering in treatment: 15066095 INFO @ Sun, 21 Jun 2020 19:13:31: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:13:31: #1 finished! INFO @ Sun, 21 Jun 2020 19:13:31: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:13:31: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:13:32: #2 number of paired peaks: 19 WARNING @ Sun, 21 Jun 2020 19:13:32: Too few paired peaks (19) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:13:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX287736/SRX287736.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287736/SRX287736.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287736/SRX287736.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287736/SRX287736.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 19:13:33: 8000000 INFO @ Sun, 21 Jun 2020 19:13:33: 11000000 INFO @ Sun, 21 Jun 2020 19:13:39: 9000000 INFO @ Sun, 21 Jun 2020 19:13:41: 12000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 19:13:46: 10000000 INFO @ Sun, 21 Jun 2020 19:13:49: 13000000 INFO @ Sun, 21 Jun 2020 19:13:53: 11000000 INFO @ Sun, 21 Jun 2020 19:13:57: 14000000 INFO @ Sun, 21 Jun 2020 19:14:00: 12000000 INFO @ Sun, 21 Jun 2020 19:14:05: 15000000 INFO @ Sun, 21 Jun 2020 19:14:06: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 19:14:06: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 19:14:06: #1 total tags in treatment: 15066114 INFO @ Sun, 21 Jun 2020 19:14:06: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:14:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:14:06: #1 tags after filtering in treatment: 15066095 INFO @ Sun, 21 Jun 2020 19:14:06: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:14:06: #1 finished! INFO @ Sun, 21 Jun 2020 19:14:06: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:14:06: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:14:07: 13000000 INFO @ Sun, 21 Jun 2020 19:14:07: #2 number of paired peaks: 19 WARNING @ Sun, 21 Jun 2020 19:14:07: Too few paired peaks (19) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:14:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX287736/SRX287736.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287736/SRX287736.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287736/SRX287736.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287736/SRX287736.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 19:14:13: 14000000 INFO @ Sun, 21 Jun 2020 19:14:18: 15000000 BigWig に変換しました。 INFO @ Sun, 21 Jun 2020 19:14:19: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 19:14:19: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 19:14:19: #1 total tags in treatment: 15066114 INFO @ Sun, 21 Jun 2020 19:14:19: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:14:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:14:19: #1 tags after filtering in treatment: 15066095 INFO @ Sun, 21 Jun 2020 19:14:19: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:14:19: #1 finished! INFO @ Sun, 21 Jun 2020 19:14:19: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:14:19: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:14:20: #2 number of paired peaks: 19 WARNING @ Sun, 21 Jun 2020 19:14:20: Too few paired peaks (19) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:14:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX287736/SRX287736.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287736/SRX287736.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287736/SRX287736.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287736/SRX287736.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling