Job ID = 6455374 SRX = SRX287735 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T10:15:06 prefetch.2.10.7: 1) Downloading 'SRR869924'... 2020-06-21T10:15:06 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T10:18:33 prefetch.2.10.7: HTTPS download succeed 2020-06-21T10:18:33 prefetch.2.10.7: 1) 'SRR869924' was downloaded successfully Read 19108114 spots for SRR869924/SRR869924.sra Written 19108114 spots for SRR869924/SRR869924.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:11 19108114 reads; of these: 19108114 (100.00%) were unpaired; of these: 1114300 (5.83%) aligned 0 times 16095128 (84.23%) aligned exactly 1 time 1898686 (9.94%) aligned >1 times 94.17% overall alignment rate Time searching: 00:04:11 Overall time: 00:04:11 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3473114 / 17993814 = 0.1930 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:29:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX287735/SRX287735.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX287735/SRX287735.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX287735/SRX287735.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX287735/SRX287735.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:29:40: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:29:40: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:29:45: 1000000 INFO @ Sun, 21 Jun 2020 19:29:51: 2000000 INFO @ Sun, 21 Jun 2020 19:29:56: 3000000 INFO @ Sun, 21 Jun 2020 19:30:01: 4000000 INFO @ Sun, 21 Jun 2020 19:30:06: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:30:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX287735/SRX287735.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX287735/SRX287735.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX287735/SRX287735.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX287735/SRX287735.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:30:10: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:30:10: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:30:12: 6000000 INFO @ Sun, 21 Jun 2020 19:30:16: 1000000 INFO @ Sun, 21 Jun 2020 19:30:18: 7000000 INFO @ Sun, 21 Jun 2020 19:30:22: 2000000 INFO @ Sun, 21 Jun 2020 19:30:24: 8000000 INFO @ Sun, 21 Jun 2020 19:30:29: 3000000 INFO @ Sun, 21 Jun 2020 19:30:29: 9000000 INFO @ Sun, 21 Jun 2020 19:30:35: 10000000 INFO @ Sun, 21 Jun 2020 19:30:36: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:30:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX287735/SRX287735.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX287735/SRX287735.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX287735/SRX287735.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX287735/SRX287735.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:30:40: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:30:40: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:30:41: 11000000 INFO @ Sun, 21 Jun 2020 19:30:43: 5000000 INFO @ Sun, 21 Jun 2020 19:30:47: 12000000 INFO @ Sun, 21 Jun 2020 19:30:47: 1000000 INFO @ Sun, 21 Jun 2020 19:30:50: 6000000 INFO @ Sun, 21 Jun 2020 19:30:53: 13000000 INFO @ Sun, 21 Jun 2020 19:30:55: 2000000 INFO @ Sun, 21 Jun 2020 19:30:57: 7000000 INFO @ Sun, 21 Jun 2020 19:31:00: 14000000 INFO @ Sun, 21 Jun 2020 19:31:02: 3000000 INFO @ Sun, 21 Jun 2020 19:31:04: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 19:31:04: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 19:31:04: #1 total tags in treatment: 14520700 INFO @ Sun, 21 Jun 2020 19:31:04: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:31:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:31:04: 8000000 INFO @ Sun, 21 Jun 2020 19:31:05: #1 tags after filtering in treatment: 14520683 INFO @ Sun, 21 Jun 2020 19:31:05: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:31:05: #1 finished! INFO @ Sun, 21 Jun 2020 19:31:05: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:31:05: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:31:06: #2 number of paired peaks: 35 WARNING @ Sun, 21 Jun 2020 19:31:06: Too few paired peaks (35) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:31:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX287735/SRX287735.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287735/SRX287735.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287735/SRX287735.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287735/SRX287735.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 19:31:09: 4000000 INFO @ Sun, 21 Jun 2020 19:31:11: 9000000 INFO @ Sun, 21 Jun 2020 19:31:17: 5000000 INFO @ Sun, 21 Jun 2020 19:31:18: 10000000 INFO @ Sun, 21 Jun 2020 19:31:24: 6000000 INFO @ Sun, 21 Jun 2020 19:31:25: 11000000 INFO @ Sun, 21 Jun 2020 19:31:31: 7000000 INFO @ Sun, 21 Jun 2020 19:31:33: 12000000 INFO @ Sun, 21 Jun 2020 19:31:38: 8000000 INFO @ Sun, 21 Jun 2020 19:31:40: 13000000 INFO @ Sun, 21 Jun 2020 19:31:45: 9000000 INFO @ Sun, 21 Jun 2020 19:31:48: 14000000 INFO @ Sun, 21 Jun 2020 19:31:52: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 19:31:52: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 19:31:52: #1 total tags in treatment: 14520700 INFO @ Sun, 21 Jun 2020 19:31:52: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:31:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:31:53: 10000000 INFO @ Sun, 21 Jun 2020 19:31:53: #1 tags after filtering in treatment: 14520683 INFO @ Sun, 21 Jun 2020 19:31:53: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:31:53: #1 finished! INFO @ Sun, 21 Jun 2020 19:31:53: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:31:53: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:31:54: #2 number of paired peaks: 35 WARNING @ Sun, 21 Jun 2020 19:31:54: Too few paired peaks (35) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:31:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX287735/SRX287735.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287735/SRX287735.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287735/SRX287735.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287735/SRX287735.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 19:32:00: 11000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 19:32:07: 12000000 INFO @ Sun, 21 Jun 2020 19:32:15: 13000000 INFO @ Sun, 21 Jun 2020 19:32:22: 14000000 INFO @ Sun, 21 Jun 2020 19:32:26: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 19:32:26: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 19:32:26: #1 total tags in treatment: 14520700 INFO @ Sun, 21 Jun 2020 19:32:26: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:32:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:32:26: #1 tags after filtering in treatment: 14520683 INFO @ Sun, 21 Jun 2020 19:32:26: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:32:26: #1 finished! INFO @ Sun, 21 Jun 2020 19:32:26: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:32:26: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:32:27: #2 number of paired peaks: 35 WARNING @ Sun, 21 Jun 2020 19:32:27: Too few paired peaks (35) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:32:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX287735/SRX287735.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287735/SRX287735.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287735/SRX287735.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287735/SRX287735.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。