Job ID = 6455349 SRX = SRX287715 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T09:54:55 prefetch.2.10.7: 1) Downloading 'SRR869903'... 2020-06-21T09:54:55 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T09:57:34 prefetch.2.10.7: HTTPS download succeed 2020-06-21T09:57:35 prefetch.2.10.7: 'SRR869903' is valid 2020-06-21T09:57:35 prefetch.2.10.7: 1) 'SRR869903' was downloaded successfully Read 14682496 spots for SRR869903/SRR869903.sra Written 14682496 spots for SRR869903/SRR869903.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:19 14682496 reads; of these: 14682496 (100.00%) were unpaired; of these: 1213549 (8.27%) aligned 0 times 11857522 (80.76%) aligned exactly 1 time 1611425 (10.98%) aligned >1 times 91.73% overall alignment rate Time searching: 00:03:19 Overall time: 00:03:19 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1194683 / 13468947 = 0.0887 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:05:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX287715/SRX287715.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX287715/SRX287715.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX287715/SRX287715.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX287715/SRX287715.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:05:23: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:05:23: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:05:29: 1000000 INFO @ Sun, 21 Jun 2020 19:05:35: 2000000 INFO @ Sun, 21 Jun 2020 19:05:42: 3000000 INFO @ Sun, 21 Jun 2020 19:05:48: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:05:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX287715/SRX287715.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX287715/SRX287715.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX287715/SRX287715.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX287715/SRX287715.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:05:53: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:05:53: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:05:55: 5000000 INFO @ Sun, 21 Jun 2020 19:06:00: 1000000 INFO @ Sun, 21 Jun 2020 19:06:02: 6000000 INFO @ Sun, 21 Jun 2020 19:06:07: 2000000 INFO @ Sun, 21 Jun 2020 19:06:10: 7000000 INFO @ Sun, 21 Jun 2020 19:06:14: 3000000 INFO @ Sun, 21 Jun 2020 19:06:17: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:06:21: 4000000 INFO @ Sun, 21 Jun 2020 19:06:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX287715/SRX287715.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX287715/SRX287715.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX287715/SRX287715.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX287715/SRX287715.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:06:23: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:06:23: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:06:24: 9000000 INFO @ Sun, 21 Jun 2020 19:06:29: 5000000 INFO @ Sun, 21 Jun 2020 19:06:30: 1000000 INFO @ Sun, 21 Jun 2020 19:06:31: 10000000 INFO @ Sun, 21 Jun 2020 19:06:36: 6000000 INFO @ Sun, 21 Jun 2020 19:06:37: 2000000 INFO @ Sun, 21 Jun 2020 19:06:39: 11000000 INFO @ Sun, 21 Jun 2020 19:06:43: 7000000 INFO @ Sun, 21 Jun 2020 19:06:43: 3000000 INFO @ Sun, 21 Jun 2020 19:06:47: 12000000 INFO @ Sun, 21 Jun 2020 19:06:49: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 19:06:49: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 19:06:49: #1 total tags in treatment: 12274264 INFO @ Sun, 21 Jun 2020 19:06:49: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:06:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:06:49: #1 tags after filtering in treatment: 12274241 INFO @ Sun, 21 Jun 2020 19:06:49: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:06:49: #1 finished! INFO @ Sun, 21 Jun 2020 19:06:49: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:06:49: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:06:50: 4000000 INFO @ Sun, 21 Jun 2020 19:06:50: #2 number of paired peaks: 36 WARNING @ Sun, 21 Jun 2020 19:06:50: Too few paired peaks (36) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:06:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX287715/SRX287715.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287715/SRX287715.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287715/SRX287715.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287715/SRX287715.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 19:06:51: 8000000 INFO @ Sun, 21 Jun 2020 19:06:57: 5000000 INFO @ Sun, 21 Jun 2020 19:06:58: 9000000 INFO @ Sun, 21 Jun 2020 19:07:03: 6000000 INFO @ Sun, 21 Jun 2020 19:07:05: 10000000 INFO @ Sun, 21 Jun 2020 19:07:10: 7000000 INFO @ Sun, 21 Jun 2020 19:07:12: 11000000 INFO @ Sun, 21 Jun 2020 19:07:17: 8000000 INFO @ Sun, 21 Jun 2020 19:07:20: 12000000 INFO @ Sun, 21 Jun 2020 19:07:22: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 19:07:22: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 19:07:22: #1 total tags in treatment: 12274264 INFO @ Sun, 21 Jun 2020 19:07:22: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:07:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:07:22: #1 tags after filtering in treatment: 12274241 INFO @ Sun, 21 Jun 2020 19:07:22: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:07:22: #1 finished! INFO @ Sun, 21 Jun 2020 19:07:22: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:07:22: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:07:23: 9000000 INFO @ Sun, 21 Jun 2020 19:07:23: #2 number of paired peaks: 36 WARNING @ Sun, 21 Jun 2020 19:07:23: Too few paired peaks (36) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:07:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX287715/SRX287715.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287715/SRX287715.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287715/SRX287715.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287715/SRX287715.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 19:07:29: 10000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 19:07:35: 11000000 INFO @ Sun, 21 Jun 2020 19:07:40: 12000000 INFO @ Sun, 21 Jun 2020 19:07:42: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 19:07:42: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 19:07:42: #1 total tags in treatment: 12274264 INFO @ Sun, 21 Jun 2020 19:07:42: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:07:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:07:42: #1 tags after filtering in treatment: 12274241 INFO @ Sun, 21 Jun 2020 19:07:42: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:07:42: #1 finished! INFO @ Sun, 21 Jun 2020 19:07:42: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:07:42: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:07:43: #2 number of paired peaks: 36 WARNING @ Sun, 21 Jun 2020 19:07:43: Too few paired peaks (36) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:07:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX287715/SRX287715.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287715/SRX287715.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287715/SRX287715.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX287715/SRX287715.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。