Job ID = 6455210 SRX = SRX2870710 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T09:57:55 prefetch.2.10.7: 1) Downloading 'SRR5630473'... 2020-06-21T09:57:55 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T10:00:26 prefetch.2.10.7: HTTPS download succeed 2020-06-21T10:00:27 prefetch.2.10.7: 'SRR5630473' is valid 2020-06-21T10:00:27 prefetch.2.10.7: 1) 'SRR5630473' was downloaded successfully 2020-06-21T10:00:27 prefetch.2.10.7: 'SRR5630473' has 0 unresolved dependencies Read 18583760 spots for SRR5630473/SRR5630473.sra Written 18583760 spots for SRR5630473/SRR5630473.sra 2020-06-21T10:01:35 prefetch.2.10.7: 1) Downloading 'SRR5630475'... 2020-06-21T10:01:35 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T10:03:33 prefetch.2.10.7: HTTPS download succeed 2020-06-21T10:03:34 prefetch.2.10.7: 'SRR5630475' is valid 2020-06-21T10:03:34 prefetch.2.10.7: 1) 'SRR5630475' was downloaded successfully 2020-06-21T10:03:34 prefetch.2.10.7: 'SRR5630475' has 0 unresolved dependencies Read 18534700 spots for SRR5630475/SRR5630475.sra Written 18534700 spots for SRR5630475/SRR5630475.sra 2020-06-21T10:04:42 prefetch.2.10.7: 1) Downloading 'SRR5630476'... 2020-06-21T10:04:42 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T10:07:26 prefetch.2.10.7: HTTPS download succeed 2020-06-21T10:07:27 prefetch.2.10.7: 'SRR5630476' is valid 2020-06-21T10:07:27 prefetch.2.10.7: 1) 'SRR5630476' was downloaded successfully 2020-06-21T10:07:27 prefetch.2.10.7: 'SRR5630476' has 0 unresolved dependencies Read 18441147 spots for SRR5630476/SRR5630476.sra Written 18441147 spots for SRR5630476/SRR5630476.sra 2020-06-21T10:08:35 prefetch.2.10.7: 1) Downloading 'SRR5630477'... 2020-06-21T10:08:35 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T10:10:44 prefetch.2.10.7: HTTPS download succeed 2020-06-21T10:10:45 prefetch.2.10.7: 'SRR5630477' is valid 2020-06-21T10:10:45 prefetch.2.10.7: 1) 'SRR5630477' was downloaded successfully 2020-06-21T10:10:45 prefetch.2.10.7: 'SRR5630477' has 0 unresolved dependencies Read 18457197 spots for SRR5630477/SRR5630477.sra Written 18457197 spots for SRR5630477/SRR5630477.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:30:20 74016804 reads; of these: 74016804 (100.00%) were unpaired; of these: 3030320 (4.09%) aligned 0 times 49917277 (67.44%) aligned exactly 1 time 21069207 (28.47%) aligned >1 times 95.91% overall alignment rate Time searching: 00:30:20 Overall time: 00:30:20 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 32 files... [bam_rmdupse_core] 20676545 / 70986484 = 0.2913 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:58:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX2870710/SRX2870710.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX2870710/SRX2870710.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX2870710/SRX2870710.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX2870710/SRX2870710.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:58:54: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:58:54: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:58:59: 1000000 INFO @ Sun, 21 Jun 2020 19:59:04: 2000000 INFO @ Sun, 21 Jun 2020 19:59:10: 3000000 INFO @ Sun, 21 Jun 2020 19:59:15: 4000000 INFO @ Sun, 21 Jun 2020 19:59:21: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:59:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX2870710/SRX2870710.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX2870710/SRX2870710.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX2870710/SRX2870710.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX2870710/SRX2870710.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:59:24: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:59:24: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:59:26: 6000000 INFO @ Sun, 21 Jun 2020 19:59:29: 1000000 INFO @ Sun, 21 Jun 2020 19:59:32: 7000000 INFO @ Sun, 21 Jun 2020 19:59:35: 2000000 INFO @ Sun, 21 Jun 2020 19:59:38: 8000000 INFO @ Sun, 21 Jun 2020 19:59:41: 3000000 INFO @ Sun, 21 Jun 2020 19:59:43: 9000000 INFO @ Sun, 21 Jun 2020 19:59:47: 4000000 INFO @ Sun, 21 Jun 2020 19:59:49: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:59:52: 5000000 INFO @ Sun, 21 Jun 2020 19:59:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX2870710/SRX2870710.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX2870710/SRX2870710.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX2870710/SRX2870710.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX2870710/SRX2870710.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:59:54: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:59:54: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:59:55: 11000000 INFO @ Sun, 21 Jun 2020 19:59:59: 6000000 INFO @ Sun, 21 Jun 2020 20:00:00: 1000000 INFO @ Sun, 21 Jun 2020 20:00:01: 12000000 INFO @ Sun, 21 Jun 2020 20:00:05: 7000000 INFO @ Sun, 21 Jun 2020 20:00:07: 2000000 INFO @ Sun, 21 Jun 2020 20:00:07: 13000000 INFO @ Sun, 21 Jun 2020 20:00:11: 8000000 INFO @ Sun, 21 Jun 2020 20:00:13: 3000000 INFO @ Sun, 21 Jun 2020 20:00:14: 14000000 INFO @ Sun, 21 Jun 2020 20:00:17: 9000000 INFO @ Sun, 21 Jun 2020 20:00:19: 4000000 INFO @ Sun, 21 Jun 2020 20:00:20: 15000000 INFO @ Sun, 21 Jun 2020 20:00:23: 10000000 INFO @ Sun, 21 Jun 2020 20:00:25: 5000000 INFO @ Sun, 21 Jun 2020 20:00:26: 16000000 INFO @ Sun, 21 Jun 2020 20:00:29: 11000000 INFO @ Sun, 21 Jun 2020 20:00:32: 17000000 INFO @ Sun, 21 Jun 2020 20:00:32: 6000000 INFO @ Sun, 21 Jun 2020 20:00:35: 12000000 INFO @ Sun, 21 Jun 2020 20:00:39: 18000000 INFO @ Sun, 21 Jun 2020 20:00:40: 7000000 INFO @ Sun, 21 Jun 2020 20:00:42: 13000000 INFO @ Sun, 21 Jun 2020 20:00:45: 19000000 INFO @ Sun, 21 Jun 2020 20:00:47: 8000000 INFO @ Sun, 21 Jun 2020 20:00:48: 14000000 INFO @ Sun, 21 Jun 2020 20:00:51: 20000000 INFO @ Sun, 21 Jun 2020 20:00:54: 9000000 INFO @ Sun, 21 Jun 2020 20:00:54: 15000000 INFO @ Sun, 21 Jun 2020 20:00:57: 21000000 INFO @ Sun, 21 Jun 2020 20:01:01: 16000000 INFO @ Sun, 21 Jun 2020 20:01:01: 10000000 INFO @ Sun, 21 Jun 2020 20:01:03: 22000000 INFO @ Sun, 21 Jun 2020 20:01:07: 17000000 INFO @ Sun, 21 Jun 2020 20:01:08: 11000000 INFO @ Sun, 21 Jun 2020 20:01:10: 23000000 INFO @ Sun, 21 Jun 2020 20:01:13: 18000000 INFO @ Sun, 21 Jun 2020 20:01:16: 12000000 INFO @ Sun, 21 Jun 2020 20:01:16: 24000000 INFO @ Sun, 21 Jun 2020 20:01:19: 19000000 INFO @ Sun, 21 Jun 2020 20:01:22: 13000000 INFO @ Sun, 21 Jun 2020 20:01:22: 25000000 INFO @ Sun, 21 Jun 2020 20:01:26: 20000000 INFO @ Sun, 21 Jun 2020 20:01:28: 14000000 INFO @ Sun, 21 Jun 2020 20:01:28: 26000000 INFO @ Sun, 21 Jun 2020 20:01:32: 21000000 INFO @ Sun, 21 Jun 2020 20:01:34: 27000000 INFO @ Sun, 21 Jun 2020 20:01:34: 15000000 INFO @ Sun, 21 Jun 2020 20:01:38: 22000000 INFO @ Sun, 21 Jun 2020 20:01:40: 28000000 INFO @ Sun, 21 Jun 2020 20:01:40: 16000000 INFO @ Sun, 21 Jun 2020 20:01:44: 23000000 INFO @ Sun, 21 Jun 2020 20:01:46: 29000000 INFO @ Sun, 21 Jun 2020 20:01:46: 17000000 INFO @ Sun, 21 Jun 2020 20:01:50: 24000000 INFO @ Sun, 21 Jun 2020 20:01:52: 30000000 INFO @ Sun, 21 Jun 2020 20:01:52: 18000000 INFO @ Sun, 21 Jun 2020 20:01:56: 25000000 INFO @ Sun, 21 Jun 2020 20:01:58: 31000000 INFO @ Sun, 21 Jun 2020 20:01:58: 19000000 INFO @ Sun, 21 Jun 2020 20:02:02: 26000000 INFO @ Sun, 21 Jun 2020 20:02:03: 32000000 INFO @ Sun, 21 Jun 2020 20:02:04: 20000000 INFO @ Sun, 21 Jun 2020 20:02:08: 27000000 INFO @ Sun, 21 Jun 2020 20:02:09: 33000000 INFO @ Sun, 21 Jun 2020 20:02:10: 21000000 INFO @ Sun, 21 Jun 2020 20:02:14: 28000000 INFO @ Sun, 21 Jun 2020 20:02:15: 34000000 INFO @ Sun, 21 Jun 2020 20:02:16: 22000000 INFO @ Sun, 21 Jun 2020 20:02:20: 29000000 INFO @ Sun, 21 Jun 2020 20:02:21: 35000000 INFO @ Sun, 21 Jun 2020 20:02:22: 23000000 INFO @ Sun, 21 Jun 2020 20:02:25: 30000000 INFO @ Sun, 21 Jun 2020 20:02:27: 36000000 INFO @ Sun, 21 Jun 2020 20:02:28: 24000000 INFO @ Sun, 21 Jun 2020 20:02:31: 31000000 INFO @ Sun, 21 Jun 2020 20:02:33: 37000000 INFO @ Sun, 21 Jun 2020 20:02:34: 25000000 INFO @ Sun, 21 Jun 2020 20:02:37: 32000000 INFO @ Sun, 21 Jun 2020 20:02:39: 38000000 INFO @ Sun, 21 Jun 2020 20:02:40: 26000000 INFO @ Sun, 21 Jun 2020 20:02:43: 33000000 INFO @ Sun, 21 Jun 2020 20:02:45: 39000000 INFO @ Sun, 21 Jun 2020 20:02:46: 27000000 INFO @ Sun, 21 Jun 2020 20:02:49: 34000000 INFO @ Sun, 21 Jun 2020 20:02:51: 40000000 INFO @ Sun, 21 Jun 2020 20:02:52: 28000000 INFO @ Sun, 21 Jun 2020 20:02:55: 35000000 INFO @ Sun, 21 Jun 2020 20:02:57: 41000000 INFO @ Sun, 21 Jun 2020 20:02:57: 29000000 INFO @ Sun, 21 Jun 2020 20:03:01: 36000000 INFO @ Sun, 21 Jun 2020 20:03:03: 30000000 INFO @ Sun, 21 Jun 2020 20:03:03: 42000000 INFO @ Sun, 21 Jun 2020 20:03:06: 37000000 INFO @ Sun, 21 Jun 2020 20:03:09: 31000000 INFO @ Sun, 21 Jun 2020 20:03:09: 43000000 INFO @ Sun, 21 Jun 2020 20:03:12: 38000000 INFO @ Sun, 21 Jun 2020 20:03:15: 32000000 INFO @ Sun, 21 Jun 2020 20:03:15: 44000000 INFO @ Sun, 21 Jun 2020 20:03:18: 39000000 INFO @ Sun, 21 Jun 2020 20:03:21: 45000000 INFO @ Sun, 21 Jun 2020 20:03:21: 33000000 INFO @ Sun, 21 Jun 2020 20:03:25: 40000000 INFO @ Sun, 21 Jun 2020 20:03:28: 46000000 INFO @ Sun, 21 Jun 2020 20:03:28: 34000000 INFO @ Sun, 21 Jun 2020 20:03:31: 41000000 INFO @ Sun, 21 Jun 2020 20:03:34: 47000000 INFO @ Sun, 21 Jun 2020 20:03:35: 35000000 INFO @ Sun, 21 Jun 2020 20:03:38: 42000000 INFO @ Sun, 21 Jun 2020 20:03:40: 48000000 INFO @ Sun, 21 Jun 2020 20:03:42: 36000000 INFO @ Sun, 21 Jun 2020 20:03:44: 43000000 INFO @ Sun, 21 Jun 2020 20:03:46: 49000000 INFO @ Sun, 21 Jun 2020 20:03:48: 37000000 INFO @ Sun, 21 Jun 2020 20:03:50: 44000000 INFO @ Sun, 21 Jun 2020 20:03:52: 50000000 INFO @ Sun, 21 Jun 2020 20:03:54: #1 tag size is determined as 75 bps INFO @ Sun, 21 Jun 2020 20:03:54: #1 tag size = 75 INFO @ Sun, 21 Jun 2020 20:03:54: #1 total tags in treatment: 50309939 INFO @ Sun, 21 Jun 2020 20:03:54: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:03:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:03:54: 38000000 INFO @ Sun, 21 Jun 2020 20:03:55: #1 tags after filtering in treatment: 50309931 INFO @ Sun, 21 Jun 2020 20:03:55: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:03:55: #1 finished! INFO @ Sun, 21 Jun 2020 20:03:55: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:03:55: #2 looking for paired plus/minus strand peaks... BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 20:03:56: 45000000 INFO @ Sun, 21 Jun 2020 20:03:58: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 20:03:58: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:03:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX2870710/SRX2870710.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2870710/SRX2870710.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2870710/SRX2870710.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2870710/SRX2870710.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:04:00: 39000000 INFO @ Sun, 21 Jun 2020 20:04:02: 46000000 INFO @ Sun, 21 Jun 2020 20:04:07: 40000000 INFO @ Sun, 21 Jun 2020 20:04:08: 47000000 INFO @ Sun, 21 Jun 2020 20:04:13: 41000000 INFO @ Sun, 21 Jun 2020 20:04:14: 48000000 INFO @ Sun, 21 Jun 2020 20:04:19: 42000000 INFO @ Sun, 21 Jun 2020 20:04:20: 49000000 INFO @ Sun, 21 Jun 2020 20:04:25: 43000000 INFO @ Sun, 21 Jun 2020 20:04:26: 50000000 INFO @ Sun, 21 Jun 2020 20:04:28: #1 tag size is determined as 75 bps INFO @ Sun, 21 Jun 2020 20:04:28: #1 tag size = 75 INFO @ Sun, 21 Jun 2020 20:04:28: #1 total tags in treatment: 50309939 INFO @ Sun, 21 Jun 2020 20:04:28: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:04:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:04:29: #1 tags after filtering in treatment: 50309931 INFO @ Sun, 21 Jun 2020 20:04:29: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:04:29: #1 finished! INFO @ Sun, 21 Jun 2020 20:04:29: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:04:29: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:04:31: 44000000 INFO @ Sun, 21 Jun 2020 20:04:32: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 20:04:32: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:04:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX2870710/SRX2870710.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2870710/SRX2870710.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2870710/SRX2870710.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2870710/SRX2870710.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 20:04:37: 45000000 INFO @ Sun, 21 Jun 2020 20:04:42: 46000000 INFO @ Sun, 21 Jun 2020 20:04:48: 47000000 INFO @ Sun, 21 Jun 2020 20:04:54: 48000000 INFO @ Sun, 21 Jun 2020 20:04:59: 49000000 INFO @ Sun, 21 Jun 2020 20:05:05: 50000000 INFO @ Sun, 21 Jun 2020 20:05:07: #1 tag size is determined as 75 bps INFO @ Sun, 21 Jun 2020 20:05:07: #1 tag size = 75 INFO @ Sun, 21 Jun 2020 20:05:07: #1 total tags in treatment: 50309939 INFO @ Sun, 21 Jun 2020 20:05:07: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 20:05:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 20:05:09: #1 tags after filtering in treatment: 50309931 INFO @ Sun, 21 Jun 2020 20:05:09: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 20:05:09: #1 finished! INFO @ Sun, 21 Jun 2020 20:05:09: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 20:05:09: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 20:05:12: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 20:05:12: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 20:05:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX2870710/SRX2870710.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2870710/SRX2870710.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2870710/SRX2870710.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2870710/SRX2870710.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。