Job ID = 6455168 SRX = SRX2804226 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T09:53:40 prefetch.2.10.7: 1) Downloading 'SRR5534654'... 2020-06-21T09:53:40 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T09:59:47 prefetch.2.10.7: HTTPS download succeed 2020-06-21T09:59:47 prefetch.2.10.7: 1) 'SRR5534654' was downloaded successfully 2020-06-21T09:59:47 prefetch.2.10.7: 'SRR5534654' has 0 unresolved dependencies Read 70289234 spots for SRR5534654/SRR5534654.sra Written 70289234 spots for SRR5534654/SRR5534654.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:19:45 70289234 reads; of these: 70289234 (100.00%) were unpaired; of these: 2684898 (3.82%) aligned 0 times 46471779 (66.12%) aligned exactly 1 time 21132557 (30.07%) aligned >1 times 96.18% overall alignment rate Time searching: 00:19:45 Overall time: 00:19:45 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 28 files... [bam_rmdupse_core] 14833813 / 67604336 = 0.2194 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:35:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX2804226/SRX2804226.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX2804226/SRX2804226.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX2804226/SRX2804226.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX2804226/SRX2804226.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:35:59: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:35:59: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:36:05: 1000000 INFO @ Sun, 21 Jun 2020 19:36:11: 2000000 INFO @ Sun, 21 Jun 2020 19:36:17: 3000000 INFO @ Sun, 21 Jun 2020 19:36:23: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:36:29: 5000000 INFO @ Sun, 21 Jun 2020 19:36:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX2804226/SRX2804226.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX2804226/SRX2804226.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX2804226/SRX2804226.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX2804226/SRX2804226.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:36:29: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:36:29: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:36:35: 6000000 INFO @ Sun, 21 Jun 2020 19:36:36: 1000000 INFO @ Sun, 21 Jun 2020 19:36:42: 7000000 INFO @ Sun, 21 Jun 2020 19:36:42: 2000000 INFO @ Sun, 21 Jun 2020 19:36:49: 8000000 INFO @ Sun, 21 Jun 2020 19:36:49: 3000000 INFO @ Sun, 21 Jun 2020 19:36:55: 9000000 INFO @ Sun, 21 Jun 2020 19:36:56: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:36:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX2804226/SRX2804226.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX2804226/SRX2804226.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX2804226/SRX2804226.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX2804226/SRX2804226.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:36:59: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:36:59: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:37:02: 10000000 INFO @ Sun, 21 Jun 2020 19:37:03: 5000000 INFO @ Sun, 21 Jun 2020 19:37:06: 1000000 INFO @ Sun, 21 Jun 2020 19:37:09: 11000000 INFO @ Sun, 21 Jun 2020 19:37:09: 6000000 INFO @ Sun, 21 Jun 2020 19:37:13: 2000000 INFO @ Sun, 21 Jun 2020 19:37:16: 12000000 INFO @ Sun, 21 Jun 2020 19:37:16: 7000000 INFO @ Sun, 21 Jun 2020 19:37:20: 3000000 INFO @ Sun, 21 Jun 2020 19:37:23: 8000000 INFO @ Sun, 21 Jun 2020 19:37:23: 13000000 INFO @ Sun, 21 Jun 2020 19:37:27: 4000000 INFO @ Sun, 21 Jun 2020 19:37:30: 9000000 INFO @ Sun, 21 Jun 2020 19:37:30: 14000000 INFO @ Sun, 21 Jun 2020 19:37:33: 5000000 INFO @ Sun, 21 Jun 2020 19:37:36: 10000000 INFO @ Sun, 21 Jun 2020 19:37:37: 15000000 INFO @ Sun, 21 Jun 2020 19:37:40: 6000000 INFO @ Sun, 21 Jun 2020 19:37:43: 11000000 INFO @ Sun, 21 Jun 2020 19:37:43: 16000000 INFO @ Sun, 21 Jun 2020 19:37:47: 7000000 INFO @ Sun, 21 Jun 2020 19:37:50: 12000000 INFO @ Sun, 21 Jun 2020 19:37:50: 17000000 INFO @ Sun, 21 Jun 2020 19:37:54: 8000000 INFO @ Sun, 21 Jun 2020 19:37:57: 13000000 INFO @ Sun, 21 Jun 2020 19:37:57: 18000000 INFO @ Sun, 21 Jun 2020 19:38:01: 9000000 INFO @ Sun, 21 Jun 2020 19:38:04: 14000000 INFO @ Sun, 21 Jun 2020 19:38:04: 19000000 INFO @ Sun, 21 Jun 2020 19:38:08: 10000000 INFO @ Sun, 21 Jun 2020 19:38:11: 15000000 INFO @ Sun, 21 Jun 2020 19:38:12: 20000000 INFO @ Sun, 21 Jun 2020 19:38:15: 11000000 INFO @ Sun, 21 Jun 2020 19:38:18: 16000000 INFO @ Sun, 21 Jun 2020 19:38:19: 21000000 INFO @ Sun, 21 Jun 2020 19:38:22: 12000000 INFO @ Sun, 21 Jun 2020 19:38:25: 17000000 INFO @ Sun, 21 Jun 2020 19:38:26: 22000000 INFO @ Sun, 21 Jun 2020 19:38:29: 13000000 INFO @ Sun, 21 Jun 2020 19:38:32: 18000000 INFO @ Sun, 21 Jun 2020 19:38:33: 23000000 INFO @ Sun, 21 Jun 2020 19:38:36: 14000000 INFO @ Sun, 21 Jun 2020 19:38:39: 19000000 INFO @ Sun, 21 Jun 2020 19:38:39: 24000000 INFO @ Sun, 21 Jun 2020 19:38:43: 15000000 INFO @ Sun, 21 Jun 2020 19:38:45: 20000000 INFO @ Sun, 21 Jun 2020 19:38:46: 25000000 INFO @ Sun, 21 Jun 2020 19:38:50: 16000000 INFO @ Sun, 21 Jun 2020 19:38:52: 21000000 INFO @ Sun, 21 Jun 2020 19:38:53: 26000000 INFO @ Sun, 21 Jun 2020 19:38:57: 17000000 INFO @ Sun, 21 Jun 2020 19:38:59: 22000000 INFO @ Sun, 21 Jun 2020 19:39:00: 27000000 INFO @ Sun, 21 Jun 2020 19:39:03: 18000000 INFO @ Sun, 21 Jun 2020 19:39:05: 23000000 INFO @ Sun, 21 Jun 2020 19:39:07: 28000000 INFO @ Sun, 21 Jun 2020 19:39:10: 19000000 INFO @ Sun, 21 Jun 2020 19:39:12: 24000000 INFO @ Sun, 21 Jun 2020 19:39:13: 29000000 INFO @ Sun, 21 Jun 2020 19:39:17: 20000000 INFO @ Sun, 21 Jun 2020 19:39:19: 25000000 INFO @ Sun, 21 Jun 2020 19:39:20: 30000000 INFO @ Sun, 21 Jun 2020 19:39:24: 21000000 INFO @ Sun, 21 Jun 2020 19:39:25: 26000000 INFO @ Sun, 21 Jun 2020 19:39:27: 31000000 INFO @ Sun, 21 Jun 2020 19:39:30: 22000000 INFO @ Sun, 21 Jun 2020 19:39:32: 27000000 INFO @ Sun, 21 Jun 2020 19:39:34: 32000000 INFO @ Sun, 21 Jun 2020 19:39:37: 23000000 INFO @ Sun, 21 Jun 2020 19:39:38: 28000000 INFO @ Sun, 21 Jun 2020 19:39:40: 33000000 INFO @ Sun, 21 Jun 2020 19:39:44: 24000000 INFO @ Sun, 21 Jun 2020 19:39:45: 29000000 INFO @ Sun, 21 Jun 2020 19:39:47: 34000000 INFO @ Sun, 21 Jun 2020 19:39:51: 25000000 INFO @ Sun, 21 Jun 2020 19:39:52: 30000000 INFO @ Sun, 21 Jun 2020 19:39:54: 35000000 INFO @ Sun, 21 Jun 2020 19:39:57: 26000000 INFO @ Sun, 21 Jun 2020 19:39:58: 31000000 INFO @ Sun, 21 Jun 2020 19:40:00: 36000000 INFO @ Sun, 21 Jun 2020 19:40:04: 27000000 INFO @ Sun, 21 Jun 2020 19:40:05: 32000000 INFO @ Sun, 21 Jun 2020 19:40:07: 37000000 INFO @ Sun, 21 Jun 2020 19:40:11: 28000000 INFO @ Sun, 21 Jun 2020 19:40:11: 33000000 INFO @ Sun, 21 Jun 2020 19:40:14: 38000000 INFO @ Sun, 21 Jun 2020 19:40:18: 29000000 INFO @ Sun, 21 Jun 2020 19:40:18: 34000000 INFO @ Sun, 21 Jun 2020 19:40:20: 39000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 19:40:24: 30000000 INFO @ Sun, 21 Jun 2020 19:40:25: 35000000 INFO @ Sun, 21 Jun 2020 19:40:27: 40000000 INFO @ Sun, 21 Jun 2020 19:40:31: 31000000 INFO @ Sun, 21 Jun 2020 19:40:31: 36000000 INFO @ Sun, 21 Jun 2020 19:40:34: 41000000 INFO @ Sun, 21 Jun 2020 19:40:37: 32000000 INFO @ Sun, 21 Jun 2020 19:40:38: 37000000 INFO @ Sun, 21 Jun 2020 19:40:40: 42000000 INFO @ Sun, 21 Jun 2020 19:40:44: 33000000 INFO @ Sun, 21 Jun 2020 19:40:44: 38000000 INFO @ Sun, 21 Jun 2020 19:40:48: 43000000 INFO @ Sun, 21 Jun 2020 19:40:51: 39000000 INFO @ Sun, 21 Jun 2020 19:40:51: 34000000 INFO @ Sun, 21 Jun 2020 19:40:55: 44000000 INFO @ Sun, 21 Jun 2020 19:40:58: 40000000 INFO @ Sun, 21 Jun 2020 19:40:59: 35000000 INFO @ Sun, 21 Jun 2020 19:41:02: 45000000 INFO @ Sun, 21 Jun 2020 19:41:06: 41000000 INFO @ Sun, 21 Jun 2020 19:41:06: 36000000 INFO @ Sun, 21 Jun 2020 19:41:10: 46000000 INFO @ Sun, 21 Jun 2020 19:41:13: 42000000 INFO @ Sun, 21 Jun 2020 19:41:13: 37000000 INFO @ Sun, 21 Jun 2020 19:41:17: 47000000 INFO @ Sun, 21 Jun 2020 19:41:20: 43000000 INFO @ Sun, 21 Jun 2020 19:41:21: 38000000 INFO @ Sun, 21 Jun 2020 19:41:24: 48000000 INFO @ Sun, 21 Jun 2020 19:41:27: 44000000 INFO @ Sun, 21 Jun 2020 19:41:28: 39000000 INFO @ Sun, 21 Jun 2020 19:41:32: 49000000 INFO @ Sun, 21 Jun 2020 19:41:34: 45000000 INFO @ Sun, 21 Jun 2020 19:41:35: 40000000 INFO @ Sun, 21 Jun 2020 19:41:39: 50000000 INFO @ Sun, 21 Jun 2020 19:41:42: 46000000 INFO @ Sun, 21 Jun 2020 19:41:42: 41000000 INFO @ Sun, 21 Jun 2020 19:41:46: 51000000 INFO @ Sun, 21 Jun 2020 19:41:49: 47000000 INFO @ Sun, 21 Jun 2020 19:41:49: 42000000 INFO @ Sun, 21 Jun 2020 19:41:54: 52000000 BigWig に変換しました。 INFO @ Sun, 21 Jun 2020 19:41:56: 48000000 INFO @ Sun, 21 Jun 2020 19:41:57: 43000000 INFO @ Sun, 21 Jun 2020 19:42:00: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 19:42:00: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 19:42:00: #1 total tags in treatment: 52770523 INFO @ Sun, 21 Jun 2020 19:42:00: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:42:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:42:01: #1 tags after filtering in treatment: 52770523 INFO @ Sun, 21 Jun 2020 19:42:01: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:42:01: #1 finished! INFO @ Sun, 21 Jun 2020 19:42:01: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:42:01: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:42:03: 49000000 INFO @ Sun, 21 Jun 2020 19:42:04: 44000000 INFO @ Sun, 21 Jun 2020 19:42:05: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 19:42:05: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:42:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX2804226/SRX2804226.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2804226/SRX2804226.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2804226/SRX2804226.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2804226/SRX2804226.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 19:42:10: 50000000 INFO @ Sun, 21 Jun 2020 19:42:11: 45000000 INFO @ Sun, 21 Jun 2020 19:42:17: 51000000 INFO @ Sun, 21 Jun 2020 19:42:18: 46000000 INFO @ Sun, 21 Jun 2020 19:42:25: 52000000 INFO @ Sun, 21 Jun 2020 19:42:25: 47000000 INFO @ Sun, 21 Jun 2020 19:42:30: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 19:42:30: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 19:42:30: #1 total tags in treatment: 52770523 INFO @ Sun, 21 Jun 2020 19:42:30: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:42:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:42:31: #1 tags after filtering in treatment: 52770523 INFO @ Sun, 21 Jun 2020 19:42:31: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:42:31: #1 finished! INFO @ Sun, 21 Jun 2020 19:42:31: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:42:31: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:42:32: 48000000 INFO @ Sun, 21 Jun 2020 19:42:35: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 19:42:35: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:42:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX2804226/SRX2804226.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2804226/SRX2804226.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2804226/SRX2804226.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2804226/SRX2804226.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 19:42:38: 49000000 INFO @ Sun, 21 Jun 2020 19:42:44: 50000000 INFO @ Sun, 21 Jun 2020 19:42:50: 51000000 INFO @ Sun, 21 Jun 2020 19:42:57: 52000000 INFO @ Sun, 21 Jun 2020 19:43:02: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 19:43:02: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 19:43:02: #1 total tags in treatment: 52770523 INFO @ Sun, 21 Jun 2020 19:43:02: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:43:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:43:03: #1 tags after filtering in treatment: 52770523 INFO @ Sun, 21 Jun 2020 19:43:03: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:43:03: #1 finished! INFO @ Sun, 21 Jun 2020 19:43:03: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:43:03: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:43:06: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 19:43:06: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:43:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX2804226/SRX2804226.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2804226/SRX2804226.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2804226/SRX2804226.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2804226/SRX2804226.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling