Job ID = 6455166 SRX = SRX2804224 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T10:03:10 prefetch.2.10.7: 1) Downloading 'SRR5534652'... 2020-06-21T10:03:10 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T10:10:26 prefetch.2.10.7: HTTPS download succeed 2020-06-21T10:10:26 prefetch.2.10.7: 1) 'SRR5534652' was downloaded successfully 2020-06-21T10:10:26 prefetch.2.10.7: 'SRR5534652' has 0 unresolved dependencies Read 73470377 spots for SRR5534652/SRR5534652.sra Written 73470377 spots for SRR5534652/SRR5534652.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:23:28 73470377 reads; of these: 73470377 (100.00%) were unpaired; of these: 2567546 (3.49%) aligned 0 times 48502315 (66.02%) aligned exactly 1 time 22400516 (30.49%) aligned >1 times 96.51% overall alignment rate Time searching: 00:23:28 Overall time: 00:23:28 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 32 files... [bam_rmdupse_core] 16192952 / 70902831 = 0.2284 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:50:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX2804224/SRX2804224.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX2804224/SRX2804224.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX2804224/SRX2804224.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX2804224/SRX2804224.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:50:22: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:50:22: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:50:29: 1000000 INFO @ Sun, 21 Jun 2020 19:50:35: 2000000 INFO @ Sun, 21 Jun 2020 19:50:41: 3000000 INFO @ Sun, 21 Jun 2020 19:50:47: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:50:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX2804224/SRX2804224.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX2804224/SRX2804224.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX2804224/SRX2804224.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX2804224/SRX2804224.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:50:52: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:50:52: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:50:53: 5000000 INFO @ Sun, 21 Jun 2020 19:50:59: 1000000 INFO @ Sun, 21 Jun 2020 19:51:00: 6000000 INFO @ Sun, 21 Jun 2020 19:51:06: 2000000 INFO @ Sun, 21 Jun 2020 19:51:07: 7000000 INFO @ Sun, 21 Jun 2020 19:51:13: 3000000 INFO @ Sun, 21 Jun 2020 19:51:14: 8000000 INFO @ Sun, 21 Jun 2020 19:51:20: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:51:20: 9000000 INFO @ Sun, 21 Jun 2020 19:51:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX2804224/SRX2804224.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX2804224/SRX2804224.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX2804224/SRX2804224.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX2804224/SRX2804224.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:51:22: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:51:22: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:51:27: 5000000 INFO @ Sun, 21 Jun 2020 19:51:28: 10000000 INFO @ Sun, 21 Jun 2020 19:51:29: 1000000 INFO @ Sun, 21 Jun 2020 19:51:35: 6000000 INFO @ Sun, 21 Jun 2020 19:51:36: 11000000 INFO @ Sun, 21 Jun 2020 19:51:37: 2000000 INFO @ Sun, 21 Jun 2020 19:51:42: 7000000 INFO @ Sun, 21 Jun 2020 19:51:43: 12000000 INFO @ Sun, 21 Jun 2020 19:51:44: 3000000 INFO @ Sun, 21 Jun 2020 19:51:49: 8000000 INFO @ Sun, 21 Jun 2020 19:51:51: 13000000 INFO @ Sun, 21 Jun 2020 19:51:51: 4000000 INFO @ Sun, 21 Jun 2020 19:51:56: 9000000 INFO @ Sun, 21 Jun 2020 19:51:58: 14000000 INFO @ Sun, 21 Jun 2020 19:51:58: 5000000 INFO @ Sun, 21 Jun 2020 19:52:03: 10000000 INFO @ Sun, 21 Jun 2020 19:52:05: 15000000 INFO @ Sun, 21 Jun 2020 19:52:05: 6000000 INFO @ Sun, 21 Jun 2020 19:52:10: 11000000 INFO @ Sun, 21 Jun 2020 19:52:12: 16000000 INFO @ Sun, 21 Jun 2020 19:52:12: 7000000 INFO @ Sun, 21 Jun 2020 19:52:17: 12000000 INFO @ Sun, 21 Jun 2020 19:52:19: 8000000 INFO @ Sun, 21 Jun 2020 19:52:19: 17000000 INFO @ Sun, 21 Jun 2020 19:52:24: 13000000 INFO @ Sun, 21 Jun 2020 19:52:26: 9000000 INFO @ Sun, 21 Jun 2020 19:52:27: 18000000 INFO @ Sun, 21 Jun 2020 19:52:31: 14000000 INFO @ Sun, 21 Jun 2020 19:52:33: 10000000 INFO @ Sun, 21 Jun 2020 19:52:34: 19000000 INFO @ Sun, 21 Jun 2020 19:52:38: 15000000 INFO @ Sun, 21 Jun 2020 19:52:40: 11000000 INFO @ Sun, 21 Jun 2020 19:52:41: 20000000 INFO @ Sun, 21 Jun 2020 19:52:45: 16000000 INFO @ Sun, 21 Jun 2020 19:52:47: 12000000 INFO @ Sun, 21 Jun 2020 19:52:47: 21000000 INFO @ Sun, 21 Jun 2020 19:52:52: 17000000 INFO @ Sun, 21 Jun 2020 19:52:54: 13000000 INFO @ Sun, 21 Jun 2020 19:52:54: 22000000 INFO @ Sun, 21 Jun 2020 19:52:58: 18000000 INFO @ Sun, 21 Jun 2020 19:53:00: 14000000 INFO @ Sun, 21 Jun 2020 19:53:01: 23000000 INFO @ Sun, 21 Jun 2020 19:53:05: 19000000 INFO @ Sun, 21 Jun 2020 19:53:07: 15000000 INFO @ Sun, 21 Jun 2020 19:53:08: 24000000 INFO @ Sun, 21 Jun 2020 19:53:12: 20000000 INFO @ Sun, 21 Jun 2020 19:53:14: 16000000 INFO @ Sun, 21 Jun 2020 19:53:15: 25000000 INFO @ Sun, 21 Jun 2020 19:53:19: 21000000 INFO @ Sun, 21 Jun 2020 19:53:20: 17000000 INFO @ Sun, 21 Jun 2020 19:53:22: 26000000 INFO @ Sun, 21 Jun 2020 19:53:25: 22000000 INFO @ Sun, 21 Jun 2020 19:53:27: 18000000 INFO @ Sun, 21 Jun 2020 19:53:29: 27000000 INFO @ Sun, 21 Jun 2020 19:53:32: 23000000 INFO @ Sun, 21 Jun 2020 19:53:34: 19000000 INFO @ Sun, 21 Jun 2020 19:53:36: 28000000 INFO @ Sun, 21 Jun 2020 19:53:39: 24000000 INFO @ Sun, 21 Jun 2020 19:53:41: 20000000 INFO @ Sun, 21 Jun 2020 19:53:43: 29000000 INFO @ Sun, 21 Jun 2020 19:53:46: 25000000 INFO @ Sun, 21 Jun 2020 19:53:48: 21000000 INFO @ Sun, 21 Jun 2020 19:53:50: 30000000 INFO @ Sun, 21 Jun 2020 19:53:53: 26000000 INFO @ Sun, 21 Jun 2020 19:53:55: 22000000 INFO @ Sun, 21 Jun 2020 19:53:57: 31000000 INFO @ Sun, 21 Jun 2020 19:54:00: 27000000 INFO @ Sun, 21 Jun 2020 19:54:02: 23000000 INFO @ Sun, 21 Jun 2020 19:54:05: 32000000 INFO @ Sun, 21 Jun 2020 19:54:07: 28000000 INFO @ Sun, 21 Jun 2020 19:54:09: 24000000 INFO @ Sun, 21 Jun 2020 19:54:12: 33000000 INFO @ Sun, 21 Jun 2020 19:54:14: 29000000 INFO @ Sun, 21 Jun 2020 19:54:16: 25000000 INFO @ Sun, 21 Jun 2020 19:54:19: 34000000 INFO @ Sun, 21 Jun 2020 19:54:21: 30000000 INFO @ Sun, 21 Jun 2020 19:54:23: 26000000 INFO @ Sun, 21 Jun 2020 19:54:26: 35000000 INFO @ Sun, 21 Jun 2020 19:54:28: 31000000 INFO @ Sun, 21 Jun 2020 19:54:30: 27000000 INFO @ Sun, 21 Jun 2020 19:54:33: 36000000 INFO @ Sun, 21 Jun 2020 19:54:35: 32000000 INFO @ Sun, 21 Jun 2020 19:54:37: 28000000 INFO @ Sun, 21 Jun 2020 19:54:40: 37000000 INFO @ Sun, 21 Jun 2020 19:54:42: 33000000 INFO @ Sun, 21 Jun 2020 19:54:44: 29000000 INFO @ Sun, 21 Jun 2020 19:54:47: 38000000 INFO @ Sun, 21 Jun 2020 19:54:49: 34000000 INFO @ Sun, 21 Jun 2020 19:54:50: 30000000 INFO @ Sun, 21 Jun 2020 19:54:54: 39000000 INFO @ Sun, 21 Jun 2020 19:54:56: 35000000 INFO @ Sun, 21 Jun 2020 19:54:57: 31000000 INFO @ Sun, 21 Jun 2020 19:55:00: 40000000 INFO @ Sun, 21 Jun 2020 19:55:03: 36000000 INFO @ Sun, 21 Jun 2020 19:55:04: 32000000 INFO @ Sun, 21 Jun 2020 19:55:07: 41000000 INFO @ Sun, 21 Jun 2020 19:55:10: 37000000 INFO @ Sun, 21 Jun 2020 19:55:11: 33000000 INFO @ Sun, 21 Jun 2020 19:55:14: 42000000 INFO @ Sun, 21 Jun 2020 19:55:16: 38000000 INFO @ Sun, 21 Jun 2020 19:55:18: 34000000 INFO @ Sun, 21 Jun 2020 19:55:21: 43000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 19:55:23: 39000000 INFO @ Sun, 21 Jun 2020 19:55:24: 35000000 INFO @ Sun, 21 Jun 2020 19:55:28: 44000000 INFO @ Sun, 21 Jun 2020 19:55:30: 40000000 INFO @ Sun, 21 Jun 2020 19:55:31: 36000000 INFO @ Sun, 21 Jun 2020 19:55:35: 45000000 INFO @ Sun, 21 Jun 2020 19:55:37: 41000000 INFO @ Sun, 21 Jun 2020 19:55:38: 37000000 INFO @ Sun, 21 Jun 2020 19:55:42: 46000000 INFO @ Sun, 21 Jun 2020 19:55:44: 42000000 INFO @ Sun, 21 Jun 2020 19:55:45: 38000000 INFO @ Sun, 21 Jun 2020 19:55:49: 47000000 INFO @ Sun, 21 Jun 2020 19:55:51: 43000000 INFO @ Sun, 21 Jun 2020 19:55:52: 39000000 INFO @ Sun, 21 Jun 2020 19:55:56: 48000000 INFO @ Sun, 21 Jun 2020 19:55:58: 44000000 INFO @ Sun, 21 Jun 2020 19:55:59: 40000000 INFO @ Sun, 21 Jun 2020 19:56:03: 49000000 INFO @ Sun, 21 Jun 2020 19:56:05: 45000000 INFO @ Sun, 21 Jun 2020 19:56:05: 41000000 INFO @ Sun, 21 Jun 2020 19:56:09: 50000000 INFO @ Sun, 21 Jun 2020 19:56:12: 46000000 INFO @ Sun, 21 Jun 2020 19:56:12: 42000000 INFO @ Sun, 21 Jun 2020 19:56:16: 51000000 INFO @ Sun, 21 Jun 2020 19:56:19: 47000000 INFO @ Sun, 21 Jun 2020 19:56:19: 43000000 INFO @ Sun, 21 Jun 2020 19:56:23: 52000000 INFO @ Sun, 21 Jun 2020 19:56:25: 48000000 INFO @ Sun, 21 Jun 2020 19:56:26: 44000000 INFO @ Sun, 21 Jun 2020 19:56:29: 53000000 INFO @ Sun, 21 Jun 2020 19:56:32: 49000000 INFO @ Sun, 21 Jun 2020 19:56:33: 45000000 INFO @ Sun, 21 Jun 2020 19:56:36: 54000000 INFO @ Sun, 21 Jun 2020 19:56:39: 50000000 INFO @ Sun, 21 Jun 2020 19:56:39: 46000000 INFO @ Sun, 21 Jun 2020 19:56:41: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 19:56:41: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 19:56:41: #1 total tags in treatment: 54709879 INFO @ Sun, 21 Jun 2020 19:56:41: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:56:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:56:43: #1 tags after filtering in treatment: 54709879 INFO @ Sun, 21 Jun 2020 19:56:43: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:56:43: #1 finished! INFO @ Sun, 21 Jun 2020 19:56:43: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:56:43: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:56:46: 51000000 INFO @ Sun, 21 Jun 2020 19:56:46: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 19:56:46: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:56:46: Process for pairing-model is terminated! INFO @ Sun, 21 Jun 2020 19:56:46: 47000000 cut: /home/okishinya/chipatlas/results/dm6/SRX2804224/SRX2804224.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2804224/SRX2804224.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2804224/SRX2804224.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2804224/SRX2804224.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 19:56:52: 52000000 INFO @ Sun, 21 Jun 2020 19:56:53: 48000000 BigWig に変換しました。 INFO @ Sun, 21 Jun 2020 19:56:59: 53000000 INFO @ Sun, 21 Jun 2020 19:56:59: 49000000 INFO @ Sun, 21 Jun 2020 19:57:05: 54000000 INFO @ Sun, 21 Jun 2020 19:57:06: 50000000 INFO @ Sun, 21 Jun 2020 19:57:10: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 19:57:10: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 19:57:10: #1 total tags in treatment: 54709879 INFO @ Sun, 21 Jun 2020 19:57:10: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:57:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:57:12: #1 tags after filtering in treatment: 54709879 INFO @ Sun, 21 Jun 2020 19:57:12: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:57:12: #1 finished! INFO @ Sun, 21 Jun 2020 19:57:12: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:57:12: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:57:12: 51000000 INFO @ Sun, 21 Jun 2020 19:57:15: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 19:57:15: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:57:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX2804224/SRX2804224.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2804224/SRX2804224.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2804224/SRX2804224.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2804224/SRX2804224.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 19:57:19: 52000000 INFO @ Sun, 21 Jun 2020 19:57:25: 53000000 INFO @ Sun, 21 Jun 2020 19:57:31: 54000000 INFO @ Sun, 21 Jun 2020 19:57:35: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 19:57:35: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 19:57:35: #1 total tags in treatment: 54709879 INFO @ Sun, 21 Jun 2020 19:57:35: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:57:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:57:37: #1 tags after filtering in treatment: 54709879 INFO @ Sun, 21 Jun 2020 19:57:37: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:57:37: #1 finished! INFO @ Sun, 21 Jun 2020 19:57:37: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:57:37: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:57:40: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 19:57:40: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:57:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX2804224/SRX2804224.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2804224/SRX2804224.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2804224/SRX2804224.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2804224/SRX2804224.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling