Job ID = 6455161 SRX = SRX2804222 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T09:48:55 prefetch.2.10.7: 1) Downloading 'SRR5534650'... 2020-06-21T09:48:55 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T09:56:30 prefetch.2.10.7: HTTPS download succeed 2020-06-21T09:56:30 prefetch.2.10.7: 1) 'SRR5534650' was downloaded successfully 2020-06-21T09:56:30 prefetch.2.10.7: 'SRR5534650' has 0 unresolved dependencies Read 75159546 spots for SRR5534650/SRR5534650.sra Written 75159546 spots for SRR5534650/SRR5534650.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:22:08 75159546 reads; of these: 75159546 (100.00%) were unpaired; of these: 3015189 (4.01%) aligned 0 times 49352671 (65.66%) aligned exactly 1 time 22791686 (30.32%) aligned >1 times 95.99% overall alignment rate Time searching: 00:22:08 Overall time: 00:22:08 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 32 files... [bam_rmdupse_core] 16443136 / 72144357 = 0.2279 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:40:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX2804222/SRX2804222.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX2804222/SRX2804222.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX2804222/SRX2804222.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX2804222/SRX2804222.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:40:00: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:40:00: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:40:05: 1000000 INFO @ Sun, 21 Jun 2020 19:40:10: 2000000 INFO @ Sun, 21 Jun 2020 19:40:15: 3000000 INFO @ Sun, 21 Jun 2020 19:40:20: 4000000 INFO @ Sun, 21 Jun 2020 19:40:25: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:40:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX2804222/SRX2804222.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX2804222/SRX2804222.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX2804222/SRX2804222.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX2804222/SRX2804222.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:40:30: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:40:30: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:40:30: 6000000 INFO @ Sun, 21 Jun 2020 19:40:35: 7000000 INFO @ Sun, 21 Jun 2020 19:40:36: 1000000 INFO @ Sun, 21 Jun 2020 19:40:40: 8000000 INFO @ Sun, 21 Jun 2020 19:40:41: 2000000 INFO @ Sun, 21 Jun 2020 19:40:45: 9000000 INFO @ Sun, 21 Jun 2020 19:40:46: 3000000 INFO @ Sun, 21 Jun 2020 19:40:50: 10000000 INFO @ Sun, 21 Jun 2020 19:40:51: 4000000 INFO @ Sun, 21 Jun 2020 19:40:55: 11000000 INFO @ Sun, 21 Jun 2020 19:40:56: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:41:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX2804222/SRX2804222.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX2804222/SRX2804222.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX2804222/SRX2804222.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX2804222/SRX2804222.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:41:00: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:41:00: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:41:00: 12000000 INFO @ Sun, 21 Jun 2020 19:41:01: 6000000 INFO @ Sun, 21 Jun 2020 19:41:05: 7000000 INFO @ Sun, 21 Jun 2020 19:41:06: 13000000 INFO @ Sun, 21 Jun 2020 19:41:06: 1000000 INFO @ Sun, 21 Jun 2020 19:41:10: 8000000 INFO @ Sun, 21 Jun 2020 19:41:11: 14000000 INFO @ Sun, 21 Jun 2020 19:41:11: 2000000 INFO @ Sun, 21 Jun 2020 19:41:15: 9000000 INFO @ Sun, 21 Jun 2020 19:41:17: 15000000 INFO @ Sun, 21 Jun 2020 19:41:17: 3000000 INFO @ Sun, 21 Jun 2020 19:41:20: 10000000 INFO @ Sun, 21 Jun 2020 19:41:22: 16000000 INFO @ Sun, 21 Jun 2020 19:41:22: 4000000 INFO @ Sun, 21 Jun 2020 19:41:25: 11000000 INFO @ Sun, 21 Jun 2020 19:41:27: 17000000 INFO @ Sun, 21 Jun 2020 19:41:28: 5000000 INFO @ Sun, 21 Jun 2020 19:41:31: 12000000 INFO @ Sun, 21 Jun 2020 19:41:33: 18000000 INFO @ Sun, 21 Jun 2020 19:41:33: 6000000 INFO @ Sun, 21 Jun 2020 19:41:35: 13000000 INFO @ Sun, 21 Jun 2020 19:41:38: 19000000 INFO @ Sun, 21 Jun 2020 19:41:39: 7000000 INFO @ Sun, 21 Jun 2020 19:41:41: 14000000 INFO @ Sun, 21 Jun 2020 19:41:44: 20000000 INFO @ Sun, 21 Jun 2020 19:41:44: 8000000 INFO @ Sun, 21 Jun 2020 19:41:46: 15000000 INFO @ Sun, 21 Jun 2020 19:41:50: 21000000 INFO @ Sun, 21 Jun 2020 19:41:50: 9000000 INFO @ Sun, 21 Jun 2020 19:41:52: 16000000 INFO @ Sun, 21 Jun 2020 19:41:55: 22000000 INFO @ Sun, 21 Jun 2020 19:41:55: 10000000 INFO @ Sun, 21 Jun 2020 19:41:57: 17000000 INFO @ Sun, 21 Jun 2020 19:42:01: 23000000 INFO @ Sun, 21 Jun 2020 19:42:01: 11000000 INFO @ Sun, 21 Jun 2020 19:42:02: 18000000 INFO @ Sun, 21 Jun 2020 19:42:06: 24000000 INFO @ Sun, 21 Jun 2020 19:42:07: 12000000 INFO @ Sun, 21 Jun 2020 19:42:07: 19000000 INFO @ Sun, 21 Jun 2020 19:42:12: 25000000 INFO @ Sun, 21 Jun 2020 19:42:12: 20000000 INFO @ Sun, 21 Jun 2020 19:42:12: 13000000 INFO @ Sun, 21 Jun 2020 19:42:17: 21000000 INFO @ Sun, 21 Jun 2020 19:42:18: 14000000 INFO @ Sun, 21 Jun 2020 19:42:18: 26000000 INFO @ Sun, 21 Jun 2020 19:42:22: 22000000 INFO @ Sun, 21 Jun 2020 19:42:23: 15000000 INFO @ Sun, 21 Jun 2020 19:42:23: 27000000 INFO @ Sun, 21 Jun 2020 19:42:27: 23000000 INFO @ Sun, 21 Jun 2020 19:42:28: 16000000 INFO @ Sun, 21 Jun 2020 19:42:29: 28000000 INFO @ Sun, 21 Jun 2020 19:42:32: 24000000 INFO @ Sun, 21 Jun 2020 19:42:34: 17000000 INFO @ Sun, 21 Jun 2020 19:42:35: 29000000 INFO @ Sun, 21 Jun 2020 19:42:37: 25000000 INFO @ Sun, 21 Jun 2020 19:42:39: 18000000 INFO @ Sun, 21 Jun 2020 19:42:40: 30000000 INFO @ Sun, 21 Jun 2020 19:42:43: 26000000 INFO @ Sun, 21 Jun 2020 19:42:45: 19000000 INFO @ Sun, 21 Jun 2020 19:42:46: 31000000 INFO @ Sun, 21 Jun 2020 19:42:49: 27000000 INFO @ Sun, 21 Jun 2020 19:42:51: 20000000 INFO @ Sun, 21 Jun 2020 19:42:52: 32000000 INFO @ Sun, 21 Jun 2020 19:42:55: 28000000 INFO @ Sun, 21 Jun 2020 19:42:56: 21000000 INFO @ Sun, 21 Jun 2020 19:42:57: 33000000 INFO @ Sun, 21 Jun 2020 19:43:00: 29000000 INFO @ Sun, 21 Jun 2020 19:43:02: 22000000 INFO @ Sun, 21 Jun 2020 19:43:03: 34000000 INFO @ Sun, 21 Jun 2020 19:43:05: 30000000 INFO @ Sun, 21 Jun 2020 19:43:07: 23000000 INFO @ Sun, 21 Jun 2020 19:43:08: 35000000 INFO @ Sun, 21 Jun 2020 19:43:11: 31000000 INFO @ Sun, 21 Jun 2020 19:43:13: 24000000 INFO @ Sun, 21 Jun 2020 19:43:14: 36000000 INFO @ Sun, 21 Jun 2020 19:43:16: 32000000 INFO @ Sun, 21 Jun 2020 19:43:19: 25000000 INFO @ Sun, 21 Jun 2020 19:43:19: 37000000 INFO @ Sun, 21 Jun 2020 19:43:21: 33000000 INFO @ Sun, 21 Jun 2020 19:43:24: 26000000 INFO @ Sun, 21 Jun 2020 19:43:25: 38000000 INFO @ Sun, 21 Jun 2020 19:43:26: 34000000 INFO @ Sun, 21 Jun 2020 19:43:30: 27000000 INFO @ Sun, 21 Jun 2020 19:43:30: 39000000 INFO @ Sun, 21 Jun 2020 19:43:31: 35000000 INFO @ Sun, 21 Jun 2020 19:43:36: 28000000 INFO @ Sun, 21 Jun 2020 19:43:36: 40000000 INFO @ Sun, 21 Jun 2020 19:43:36: 36000000 INFO @ Sun, 21 Jun 2020 19:43:41: 41000000 INFO @ Sun, 21 Jun 2020 19:43:41: 29000000 INFO @ Sun, 21 Jun 2020 19:43:41: 37000000 INFO @ Sun, 21 Jun 2020 19:43:46: 38000000 INFO @ Sun, 21 Jun 2020 19:43:47: 30000000 INFO @ Sun, 21 Jun 2020 19:43:47: 42000000 INFO @ Sun, 21 Jun 2020 19:43:51: 39000000 INFO @ Sun, 21 Jun 2020 19:43:52: 31000000 INFO @ Sun, 21 Jun 2020 19:43:52: 43000000 INFO @ Sun, 21 Jun 2020 19:43:57: 40000000 INFO @ Sun, 21 Jun 2020 19:43:58: 32000000 INFO @ Sun, 21 Jun 2020 19:43:58: 44000000 INFO @ Sun, 21 Jun 2020 19:44:02: 41000000 INFO @ Sun, 21 Jun 2020 19:44:03: 33000000 INFO @ Sun, 21 Jun 2020 19:44:04: 45000000 INFO @ Sun, 21 Jun 2020 19:44:08: 42000000 INFO @ Sun, 21 Jun 2020 19:44:09: 34000000 INFO @ Sun, 21 Jun 2020 19:44:10: 46000000 INFO @ Sun, 21 Jun 2020 19:44:13: 43000000 INFO @ Sun, 21 Jun 2020 19:44:14: 35000000 INFO @ Sun, 21 Jun 2020 19:44:15: 47000000 INFO @ Sun, 21 Jun 2020 19:44:18: 44000000 INFO @ Sun, 21 Jun 2020 19:44:20: 36000000 INFO @ Sun, 21 Jun 2020 19:44:21: 48000000 INFO @ Sun, 21 Jun 2020 19:44:23: 45000000 INFO @ Sun, 21 Jun 2020 19:44:25: 37000000 INFO @ Sun, 21 Jun 2020 19:44:26: 49000000 INFO @ Sun, 21 Jun 2020 19:44:29: 46000000 INFO @ Sun, 21 Jun 2020 19:44:31: 38000000 INFO @ Sun, 21 Jun 2020 19:44:32: 50000000 INFO @ Sun, 21 Jun 2020 19:44:35: 47000000 INFO @ Sun, 21 Jun 2020 19:44:36: 39000000 INFO @ Sun, 21 Jun 2020 19:44:37: 51000000 INFO @ Sun, 21 Jun 2020 19:44:40: 48000000 INFO @ Sun, 21 Jun 2020 19:44:41: 40000000 INFO @ Sun, 21 Jun 2020 19:44:43: 52000000 INFO @ Sun, 21 Jun 2020 19:44:45: 49000000 INFO @ Sun, 21 Jun 2020 19:44:47: 41000000 INFO @ Sun, 21 Jun 2020 19:44:48: 53000000 INFO @ Sun, 21 Jun 2020 19:44:52: 50000000 INFO @ Sun, 21 Jun 2020 19:44:53: 42000000 INFO @ Sun, 21 Jun 2020 19:44:54: 54000000 INFO @ Sun, 21 Jun 2020 19:44:57: 51000000 INFO @ Sun, 21 Jun 2020 19:44:58: 43000000 INFO @ Sun, 21 Jun 2020 19:44:59: 55000000 INFO @ Sun, 21 Jun 2020 19:45:02: 52000000 INFO @ Sun, 21 Jun 2020 19:45:03: 44000000 INFO @ Sun, 21 Jun 2020 19:45:04: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 19:45:04: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 19:45:04: #1 total tags in treatment: 55701221 INFO @ Sun, 21 Jun 2020 19:45:04: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:45:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:45:05: #1 tags after filtering in treatment: 55701221 INFO @ Sun, 21 Jun 2020 19:45:05: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:45:05: #1 finished! INFO @ Sun, 21 Jun 2020 19:45:05: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:45:05: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:45:08: 53000000 INFO @ Sun, 21 Jun 2020 19:45:09: 45000000 INFO @ Sun, 21 Jun 2020 19:45:09: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 19:45:09: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:45:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX2804222/SRX2804222.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2804222/SRX2804222.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2804222/SRX2804222.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2804222/SRX2804222.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 19:45:13: 54000000 INFO @ Sun, 21 Jun 2020 19:45:14: 46000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 19:45:18: 55000000 INFO @ Sun, 21 Jun 2020 19:45:19: 47000000 INFO @ Sun, 21 Jun 2020 19:45:22: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 19:45:22: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 19:45:22: #1 total tags in treatment: 55701221 INFO @ Sun, 21 Jun 2020 19:45:22: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:45:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:45:24: #1 tags after filtering in treatment: 55701221 INFO @ Sun, 21 Jun 2020 19:45:24: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:45:24: #1 finished! INFO @ Sun, 21 Jun 2020 19:45:24: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:45:24: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:45:24: 48000000 INFO @ Sun, 21 Jun 2020 19:45:27: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 19:45:27: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:45:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX2804222/SRX2804222.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2804222/SRX2804222.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2804222/SRX2804222.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2804222/SRX2804222.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 19:45:29: 49000000 INFO @ Sun, 21 Jun 2020 19:45:34: 50000000 INFO @ Sun, 21 Jun 2020 19:45:39: 51000000 INFO @ Sun, 21 Jun 2020 19:45:44: 52000000 INFO @ Sun, 21 Jun 2020 19:45:49: 53000000 INFO @ Sun, 21 Jun 2020 19:45:54: 54000000 INFO @ Sun, 21 Jun 2020 19:45:59: 55000000 INFO @ Sun, 21 Jun 2020 19:46:04: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 19:46:04: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 19:46:04: #1 total tags in treatment: 55701221 INFO @ Sun, 21 Jun 2020 19:46:04: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:46:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:46:05: #1 tags after filtering in treatment: 55701221 INFO @ Sun, 21 Jun 2020 19:46:05: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:46:05: #1 finished! INFO @ Sun, 21 Jun 2020 19:46:05: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:46:05: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:46:08: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 19:46:08: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:46:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX2804222/SRX2804222.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2804222/SRX2804222.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2804222/SRX2804222.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2804222/SRX2804222.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。