Job ID = 6455107 SRX = SRX2734361 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T09:52:41 prefetch.2.10.7: 1) Downloading 'SRR5445338'... 2020-06-21T09:52:41 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T10:01:37 prefetch.2.10.7: HTTPS download succeed 2020-06-21T10:01:37 prefetch.2.10.7: 1) 'SRR5445338' was downloaded successfully 2020-06-21T10:01:37 prefetch.2.10.7: 'SRR5445338' has 0 unresolved dependencies Read 44637373 spots for SRR5445338/SRR5445338.sra Written 44637373 spots for SRR5445338/SRR5445338.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:31:11 44637373 reads; of these: 44637373 (100.00%) were unpaired; of these: 44622089 (99.97%) aligned 0 times 1648 (0.00%) aligned exactly 1 time 13636 (0.03%) aligned >1 times 0.03% overall alignment rate Time searching: 00:31:11 Overall time: 00:31:11 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_rmdupse_core] 12333 / 15284 = 0.8069 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:36:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX2734361/SRX2734361.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX2734361/SRX2734361.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX2734361/SRX2734361.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX2734361/SRX2734361.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:36:10: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:36:10: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:36:10: #1 tag size is determined as 100 bps INFO @ Sun, 21 Jun 2020 19:36:10: #1 tag size = 100 INFO @ Sun, 21 Jun 2020 19:36:10: #1 total tags in treatment: 2951 INFO @ Sun, 21 Jun 2020 19:36:10: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:36:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:36:10: #1 tags after filtering in treatment: 2907 INFO @ Sun, 21 Jun 2020 19:36:10: #1 Redundant rate of treatment: 0.01 INFO @ Sun, 21 Jun 2020 19:36:10: #1 finished! INFO @ Sun, 21 Jun 2020 19:36:10: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:36:10: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:36:10: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 19:36:10: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:36:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX2734361/SRX2734361.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2734361/SRX2734361.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2734361/SRX2734361.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2734361/SRX2734361.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:36:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX2734361/SRX2734361.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX2734361/SRX2734361.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX2734361/SRX2734361.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX2734361/SRX2734361.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:36:40: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:36:40: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:36:40: #1 tag size is determined as 100 bps INFO @ Sun, 21 Jun 2020 19:36:40: #1 tag size = 100 INFO @ Sun, 21 Jun 2020 19:36:40: #1 total tags in treatment: 2951 INFO @ Sun, 21 Jun 2020 19:36:40: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:36:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:36:40: #1 tags after filtering in treatment: 2907 INFO @ Sun, 21 Jun 2020 19:36:40: #1 Redundant rate of treatment: 0.01 INFO @ Sun, 21 Jun 2020 19:36:40: #1 finished! INFO @ Sun, 21 Jun 2020 19:36:40: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:36:40: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:36:40: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 19:36:40: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:36:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX2734361/SRX2734361.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2734361/SRX2734361.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2734361/SRX2734361.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2734361/SRX2734361.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。 INFO @ Sun, 21 Jun 2020 19:37:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX2734361/SRX2734361.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX2734361/SRX2734361.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX2734361/SRX2734361.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX2734361/SRX2734361.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:37:10: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:37:10: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:37:10: #1 tag size is determined as 100 bps INFO @ Sun, 21 Jun 2020 19:37:10: #1 tag size = 100 INFO @ Sun, 21 Jun 2020 19:37:10: #1 total tags in treatment: 2951 INFO @ Sun, 21 Jun 2020 19:37:10: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:37:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:37:10: #1 tags after filtering in treatment: 2907 INFO @ Sun, 21 Jun 2020 19:37:10: #1 Redundant rate of treatment: 0.01 INFO @ Sun, 21 Jun 2020 19:37:10: #1 finished! INFO @ Sun, 21 Jun 2020 19:37:10: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:37:10: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:37:10: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 19:37:10: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:37:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX2734361/SRX2734361.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2734361/SRX2734361.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2734361/SRX2734361.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2734361/SRX2734361.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling