Job ID = 12265054 SRX = SRX2661683 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:31 17349229 reads; of these: 17349229 (100.00%) were unpaired; of these: 1159468 (6.68%) aligned 0 times 14792524 (85.26%) aligned exactly 1 time 1397237 (8.05%) aligned >1 times 93.32% overall alignment rate Time searching: 00:04:31 Overall time: 00:04:31 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 6265739 / 16189761 = 0.3870 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 06:14:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX2661683/SRX2661683.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX2661683/SRX2661683.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX2661683/SRX2661683.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX2661683/SRX2661683.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 06:14:11: #1 read tag files... INFO @ Sat, 03 Apr 2021 06:14:11: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 06:14:20: 1000000 INFO @ Sat, 03 Apr 2021 06:14:29: 2000000 INFO @ Sat, 03 Apr 2021 06:14:38: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 06:14:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX2661683/SRX2661683.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX2661683/SRX2661683.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX2661683/SRX2661683.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX2661683/SRX2661683.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 06:14:41: #1 read tag files... INFO @ Sat, 03 Apr 2021 06:14:41: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 06:14:49: 4000000 INFO @ Sat, 03 Apr 2021 06:14:52: 1000000 INFO @ Sat, 03 Apr 2021 06:14:59: 5000000 INFO @ Sat, 03 Apr 2021 06:15:03: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 06:15:10: 6000000 INFO @ Sat, 03 Apr 2021 06:15:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX2661683/SRX2661683.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX2661683/SRX2661683.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX2661683/SRX2661683.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX2661683/SRX2661683.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 06:15:11: #1 read tag files... INFO @ Sat, 03 Apr 2021 06:15:11: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 06:15:13: 3000000 INFO @ Sat, 03 Apr 2021 06:15:20: 1000000 INFO @ Sat, 03 Apr 2021 06:15:20: 7000000 INFO @ Sat, 03 Apr 2021 06:15:24: 4000000 INFO @ Sat, 03 Apr 2021 06:15:28: 2000000 INFO @ Sat, 03 Apr 2021 06:15:30: 8000000 INFO @ Sat, 03 Apr 2021 06:15:34: 5000000 INFO @ Sat, 03 Apr 2021 06:15:36: 3000000 INFO @ Sat, 03 Apr 2021 06:15:40: 9000000 INFO @ Sat, 03 Apr 2021 06:15:43: 6000000 INFO @ Sat, 03 Apr 2021 06:15:44: 4000000 INFO @ Sat, 03 Apr 2021 06:15:50: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 06:15:50: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 06:15:50: #1 total tags in treatment: 9924022 INFO @ Sat, 03 Apr 2021 06:15:50: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 06:15:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 06:15:50: #1 tags after filtering in treatment: 9923980 INFO @ Sat, 03 Apr 2021 06:15:50: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 06:15:50: #1 finished! INFO @ Sat, 03 Apr 2021 06:15:50: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 06:15:50: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 06:15:51: #2 number of paired peaks: 2561 INFO @ Sat, 03 Apr 2021 06:15:51: start model_add_line... INFO @ Sat, 03 Apr 2021 06:15:51: start X-correlation... INFO @ Sat, 03 Apr 2021 06:15:51: end of X-cor INFO @ Sat, 03 Apr 2021 06:15:51: #2 finished! INFO @ Sat, 03 Apr 2021 06:15:51: #2 predicted fragment length is 78 bps INFO @ Sat, 03 Apr 2021 06:15:51: #2 alternative fragment length(s) may be 78 bps INFO @ Sat, 03 Apr 2021 06:15:51: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX2661683/SRX2661683.05_model.r WARNING @ Sat, 03 Apr 2021 06:15:51: #2 Since the d (78) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 06:15:51: #2 You may need to consider one of the other alternative d(s): 78 WARNING @ Sat, 03 Apr 2021 06:15:51: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 06:15:51: #3 Call peaks... INFO @ Sat, 03 Apr 2021 06:15:51: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 06:15:52: 5000000 INFO @ Sat, 03 Apr 2021 06:15:53: 7000000 INFO @ Sat, 03 Apr 2021 06:16:00: 6000000 INFO @ Sat, 03 Apr 2021 06:16:02: 8000000 INFO @ Sat, 03 Apr 2021 06:16:08: 7000000 INFO @ Sat, 03 Apr 2021 06:16:11: 9000000 INFO @ Sat, 03 Apr 2021 06:16:16: 8000000 INFO @ Sat, 03 Apr 2021 06:16:20: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 06:16:20: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 06:16:20: #1 total tags in treatment: 9924022 INFO @ Sat, 03 Apr 2021 06:16:20: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 06:16:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 06:16:21: #1 tags after filtering in treatment: 9923980 INFO @ Sat, 03 Apr 2021 06:16:21: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 06:16:21: #1 finished! INFO @ Sat, 03 Apr 2021 06:16:21: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 06:16:21: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 06:16:21: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 06:16:22: #2 number of paired peaks: 2561 INFO @ Sat, 03 Apr 2021 06:16:22: start model_add_line... INFO @ Sat, 03 Apr 2021 06:16:22: start X-correlation... INFO @ Sat, 03 Apr 2021 06:16:22: end of X-cor INFO @ Sat, 03 Apr 2021 06:16:22: #2 finished! INFO @ Sat, 03 Apr 2021 06:16:22: #2 predicted fragment length is 78 bps INFO @ Sat, 03 Apr 2021 06:16:22: #2 alternative fragment length(s) may be 78 bps INFO @ Sat, 03 Apr 2021 06:16:22: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX2661683/SRX2661683.10_model.r WARNING @ Sat, 03 Apr 2021 06:16:22: #2 Since the d (78) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 06:16:22: #2 You may need to consider one of the other alternative d(s): 78 WARNING @ Sat, 03 Apr 2021 06:16:22: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 06:16:22: #3 Call peaks... INFO @ Sat, 03 Apr 2021 06:16:22: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 06:16:25: 9000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 03 Apr 2021 06:16:32: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 06:16:32: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 06:16:32: #1 total tags in treatment: 9924022 INFO @ Sat, 03 Apr 2021 06:16:32: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 06:16:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 06:16:33: #1 tags after filtering in treatment: 9923980 INFO @ Sat, 03 Apr 2021 06:16:33: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 06:16:33: #1 finished! INFO @ Sat, 03 Apr 2021 06:16:33: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 06:16:33: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 06:16:34: #2 number of paired peaks: 2561 INFO @ Sat, 03 Apr 2021 06:16:34: start model_add_line... INFO @ Sat, 03 Apr 2021 06:16:34: start X-correlation... INFO @ Sat, 03 Apr 2021 06:16:34: end of X-cor INFO @ Sat, 03 Apr 2021 06:16:34: #2 finished! INFO @ Sat, 03 Apr 2021 06:16:34: #2 predicted fragment length is 78 bps INFO @ Sat, 03 Apr 2021 06:16:34: #2 alternative fragment length(s) may be 78 bps INFO @ Sat, 03 Apr 2021 06:16:34: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX2661683/SRX2661683.20_model.r WARNING @ Sat, 03 Apr 2021 06:16:34: #2 Since the d (78) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 06:16:34: #2 You may need to consider one of the other alternative d(s): 78 WARNING @ Sat, 03 Apr 2021 06:16:34: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 06:16:34: #3 Call peaks... INFO @ Sat, 03 Apr 2021 06:16:34: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 06:16:36: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX2661683/SRX2661683.05_peaks.xls INFO @ Sat, 03 Apr 2021 06:16:36: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX2661683/SRX2661683.05_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 06:16:36: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX2661683/SRX2661683.05_summits.bed INFO @ Sat, 03 Apr 2021 06:16:36: Done! pass1 - making usageList (154 chroms): 7 millis pass2 - checking and writing primary data (18079 records, 4 fields): 29 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 06:16:51: #3 Call peaks for each chromosome... BigWig に変換しました。 INFO @ Sat, 03 Apr 2021 06:17:04: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 06:17:05: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX2661683/SRX2661683.10_peaks.xls INFO @ Sat, 03 Apr 2021 06:17:05: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX2661683/SRX2661683.10_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 06:17:06: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX2661683/SRX2661683.10_summits.bed INFO @ Sat, 03 Apr 2021 06:17:06: Done! pass1 - making usageList (113 chroms): 7 millis pass2 - checking and writing primary data (10937 records, 4 fields): 20 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 06:17:19: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX2661683/SRX2661683.20_peaks.xls INFO @ Sat, 03 Apr 2021 06:17:19: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX2661683/SRX2661683.20_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 06:17:19: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX2661683/SRX2661683.20_summits.bed INFO @ Sat, 03 Apr 2021 06:17:19: Done! pass1 - making usageList (69 chroms): 4 millis pass2 - checking and writing primary data (3900 records, 4 fields): 12 millis CompletedMACS2peakCalling