Job ID = 12265083 SRX = SRX2661681 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:28 22963239 reads; of these: 22963239 (100.00%) were unpaired; of these: 1794800 (7.82%) aligned 0 times 19373906 (84.37%) aligned exactly 1 time 1794533 (7.81%) aligned >1 times 92.18% overall alignment rate Time searching: 00:05:28 Overall time: 00:05:28 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 9548929 / 21168439 = 0.4511 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 06:16:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX2661681/SRX2661681.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX2661681/SRX2661681.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX2661681/SRX2661681.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX2661681/SRX2661681.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 06:16:52: #1 read tag files... INFO @ Sat, 03 Apr 2021 06:16:52: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 06:17:03: 1000000 INFO @ Sat, 03 Apr 2021 06:17:14: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 06:17:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX2661681/SRX2661681.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX2661681/SRX2661681.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX2661681/SRX2661681.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX2661681/SRX2661681.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 06:17:22: #1 read tag files... INFO @ Sat, 03 Apr 2021 06:17:22: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 06:17:26: 3000000 INFO @ Sat, 03 Apr 2021 06:17:34: 1000000 INFO @ Sat, 03 Apr 2021 06:17:38: 4000000 INFO @ Sat, 03 Apr 2021 06:17:47: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 06:17:51: 5000000 INFO @ Sat, 03 Apr 2021 06:17:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX2661681/SRX2661681.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX2661681/SRX2661681.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX2661681/SRX2661681.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX2661681/SRX2661681.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 06:17:52: #1 read tag files... INFO @ Sat, 03 Apr 2021 06:17:52: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 06:18:02: 3000000 INFO @ Sat, 03 Apr 2021 06:18:05: 1000000 INFO @ Sat, 03 Apr 2021 06:18:06: 6000000 INFO @ Sat, 03 Apr 2021 06:18:15: 4000000 INFO @ Sat, 03 Apr 2021 06:18:18: 2000000 INFO @ Sat, 03 Apr 2021 06:18:19: 7000000 INFO @ Sat, 03 Apr 2021 06:18:26: 5000000 INFO @ Sat, 03 Apr 2021 06:18:31: 3000000 INFO @ Sat, 03 Apr 2021 06:18:32: 8000000 INFO @ Sat, 03 Apr 2021 06:18:40: 6000000 INFO @ Sat, 03 Apr 2021 06:18:44: 4000000 INFO @ Sat, 03 Apr 2021 06:18:45: 9000000 INFO @ Sat, 03 Apr 2021 06:18:53: 7000000 INFO @ Sat, 03 Apr 2021 06:18:56: 10000000 INFO @ Sat, 03 Apr 2021 06:18:57: 5000000 INFO @ Sat, 03 Apr 2021 06:19:03: 8000000 INFO @ Sat, 03 Apr 2021 06:19:05: 11000000 INFO @ Sat, 03 Apr 2021 06:19:09: 6000000 INFO @ Sat, 03 Apr 2021 06:19:11: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 06:19:11: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 06:19:11: #1 total tags in treatment: 11619510 INFO @ Sat, 03 Apr 2021 06:19:11: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 06:19:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 06:19:12: #1 tags after filtering in treatment: 11619485 INFO @ Sat, 03 Apr 2021 06:19:12: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 06:19:12: #1 finished! INFO @ Sat, 03 Apr 2021 06:19:12: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 06:19:12: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 06:19:14: #2 number of paired peaks: 2391 INFO @ Sat, 03 Apr 2021 06:19:14: start model_add_line... INFO @ Sat, 03 Apr 2021 06:19:14: start X-correlation... INFO @ Sat, 03 Apr 2021 06:19:14: end of X-cor INFO @ Sat, 03 Apr 2021 06:19:14: #2 finished! INFO @ Sat, 03 Apr 2021 06:19:14: #2 predicted fragment length is 75 bps INFO @ Sat, 03 Apr 2021 06:19:14: #2 alternative fragment length(s) may be 75 bps INFO @ Sat, 03 Apr 2021 06:19:14: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX2661681/SRX2661681.05_model.r WARNING @ Sat, 03 Apr 2021 06:19:14: #2 Since the d (75) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 06:19:14: #2 You may need to consider one of the other alternative d(s): 75 WARNING @ Sat, 03 Apr 2021 06:19:14: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 06:19:14: #3 Call peaks... INFO @ Sat, 03 Apr 2021 06:19:14: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 06:19:15: 9000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 03 Apr 2021 06:19:21: 7000000 INFO @ Sat, 03 Apr 2021 06:19:27: 10000000 INFO @ Sat, 03 Apr 2021 06:19:32: 8000000 INFO @ Sat, 03 Apr 2021 06:19:39: 11000000 INFO @ Sat, 03 Apr 2021 06:19:44: 9000000 INFO @ Sat, 03 Apr 2021 06:19:46: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 06:19:46: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 06:19:46: #1 total tags in treatment: 11619510 INFO @ Sat, 03 Apr 2021 06:19:46: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 06:19:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 06:19:46: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 06:19:47: #1 tags after filtering in treatment: 11619485 INFO @ Sat, 03 Apr 2021 06:19:47: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 06:19:47: #1 finished! INFO @ Sat, 03 Apr 2021 06:19:47: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 06:19:47: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 06:19:48: #2 number of paired peaks: 2391 INFO @ Sat, 03 Apr 2021 06:19:48: start model_add_line... INFO @ Sat, 03 Apr 2021 06:19:48: start X-correlation... INFO @ Sat, 03 Apr 2021 06:19:48: end of X-cor INFO @ Sat, 03 Apr 2021 06:19:48: #2 finished! INFO @ Sat, 03 Apr 2021 06:19:48: #2 predicted fragment length is 75 bps INFO @ Sat, 03 Apr 2021 06:19:48: #2 alternative fragment length(s) may be 75 bps INFO @ Sat, 03 Apr 2021 06:19:48: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX2661681/SRX2661681.10_model.r WARNING @ Sat, 03 Apr 2021 06:19:48: #2 Since the d (75) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 06:19:48: #2 You may need to consider one of the other alternative d(s): 75 WARNING @ Sat, 03 Apr 2021 06:19:48: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 06:19:48: #3 Call peaks... INFO @ Sat, 03 Apr 2021 06:19:48: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Sat, 03 Apr 2021 06:19:56: 10000000 INFO @ Sat, 03 Apr 2021 06:20:04: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX2661681/SRX2661681.05_peaks.xls INFO @ Sat, 03 Apr 2021 06:20:04: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX2661681/SRX2661681.05_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 06:20:04: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX2661681/SRX2661681.05_summits.bed INFO @ Sat, 03 Apr 2021 06:20:04: Done! pass1 - making usageList (177 chroms): 9 millis pass2 - checking and writing primary data (19962 records, 4 fields): 40 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 06:20:06: 11000000 INFO @ Sat, 03 Apr 2021 06:20:13: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 06:20:13: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 06:20:13: #1 total tags in treatment: 11619510 INFO @ Sat, 03 Apr 2021 06:20:13: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 06:20:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 06:20:14: #1 tags after filtering in treatment: 11619485 INFO @ Sat, 03 Apr 2021 06:20:14: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 06:20:14: #1 finished! INFO @ Sat, 03 Apr 2021 06:20:14: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 06:20:14: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 06:20:15: #2 number of paired peaks: 2391 INFO @ Sat, 03 Apr 2021 06:20:15: start model_add_line... INFO @ Sat, 03 Apr 2021 06:20:15: start X-correlation... INFO @ Sat, 03 Apr 2021 06:20:15: end of X-cor INFO @ Sat, 03 Apr 2021 06:20:15: #2 finished! INFO @ Sat, 03 Apr 2021 06:20:15: #2 predicted fragment length is 75 bps INFO @ Sat, 03 Apr 2021 06:20:15: #2 alternative fragment length(s) may be 75 bps INFO @ Sat, 03 Apr 2021 06:20:15: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX2661681/SRX2661681.20_model.r WARNING @ Sat, 03 Apr 2021 06:20:15: #2 Since the d (75) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 06:20:15: #2 You may need to consider one of the other alternative d(s): 75 WARNING @ Sat, 03 Apr 2021 06:20:15: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 06:20:15: #3 Call peaks... INFO @ Sat, 03 Apr 2021 06:20:15: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 06:20:23: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 06:20:40: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX2661681/SRX2661681.10_peaks.xls INFO @ Sat, 03 Apr 2021 06:20:40: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX2661681/SRX2661681.10_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 06:20:40: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX2661681/SRX2661681.10_summits.bed INFO @ Sat, 03 Apr 2021 06:20:40: Done! pass1 - making usageList (118 chroms): 3 millis pass2 - checking and writing primary data (11935 records, 4 fields): 22 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 06:20:52: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 06:21:11: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX2661681/SRX2661681.20_peaks.xls INFO @ Sat, 03 Apr 2021 06:21:11: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX2661681/SRX2661681.20_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 06:21:11: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX2661681/SRX2661681.20_summits.bed INFO @ Sat, 03 Apr 2021 06:21:11: Done! pass1 - making usageList (70 chroms): 1 millis pass2 - checking and writing primary data (4107 records, 4 fields): 11 millis CompletedMACS2peakCalling