Job ID = 6454704 SRX = SRX2520650 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T09:14:09 prefetch.2.10.7: 1) Downloading 'SRR5206769'... 2020-06-21T09:14:09 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T09:20:51 prefetch.2.10.7: HTTPS download succeed 2020-06-21T09:20:51 prefetch.2.10.7: 1) 'SRR5206769' was downloaded successfully Read 23897334 spots for SRR5206769/SRR5206769.sra Written 23897334 spots for SRR5206769/SRR5206769.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:59 23897334 reads; of these: 23897334 (100.00%) were unpaired; of these: 540818 (2.26%) aligned 0 times 16752066 (70.10%) aligned exactly 1 time 6604450 (27.64%) aligned >1 times 97.74% overall alignment rate Time searching: 00:06:59 Overall time: 00:06:59 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 6240826 / 23356516 = 0.2672 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:35:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX2520650/SRX2520650.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX2520650/SRX2520650.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX2520650/SRX2520650.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX2520650/SRX2520650.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:35:09: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:35:09: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:35:16: 1000000 INFO @ Sun, 21 Jun 2020 18:35:22: 2000000 INFO @ Sun, 21 Jun 2020 18:35:28: 3000000 INFO @ Sun, 21 Jun 2020 18:35:35: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:35:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX2520650/SRX2520650.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX2520650/SRX2520650.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX2520650/SRX2520650.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX2520650/SRX2520650.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:35:39: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:35:39: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:35:41: 5000000 INFO @ Sun, 21 Jun 2020 18:35:46: 1000000 INFO @ Sun, 21 Jun 2020 18:35:47: 6000000 INFO @ Sun, 21 Jun 2020 18:35:52: 2000000 INFO @ Sun, 21 Jun 2020 18:35:54: 7000000 INFO @ Sun, 21 Jun 2020 18:35:58: 3000000 INFO @ Sun, 21 Jun 2020 18:36:00: 8000000 INFO @ Sun, 21 Jun 2020 18:36:05: 4000000 INFO @ Sun, 21 Jun 2020 18:36:07: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:36:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX2520650/SRX2520650.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX2520650/SRX2520650.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX2520650/SRX2520650.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX2520650/SRX2520650.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:36:09: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:36:09: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:36:11: 5000000 INFO @ Sun, 21 Jun 2020 18:36:13: 10000000 INFO @ Sun, 21 Jun 2020 18:36:16: 1000000 INFO @ Sun, 21 Jun 2020 18:36:18: 6000000 INFO @ Sun, 21 Jun 2020 18:36:20: 11000000 INFO @ Sun, 21 Jun 2020 18:36:23: 2000000 INFO @ Sun, 21 Jun 2020 18:36:24: 7000000 INFO @ Sun, 21 Jun 2020 18:36:26: 12000000 INFO @ Sun, 21 Jun 2020 18:36:29: 3000000 INFO @ Sun, 21 Jun 2020 18:36:31: 8000000 INFO @ Sun, 21 Jun 2020 18:36:33: 13000000 INFO @ Sun, 21 Jun 2020 18:36:36: 4000000 INFO @ Sun, 21 Jun 2020 18:36:38: 9000000 INFO @ Sun, 21 Jun 2020 18:36:40: 14000000 INFO @ Sun, 21 Jun 2020 18:36:43: 5000000 INFO @ Sun, 21 Jun 2020 18:36:44: 10000000 INFO @ Sun, 21 Jun 2020 18:36:47: 15000000 INFO @ Sun, 21 Jun 2020 18:36:49: 6000000 INFO @ Sun, 21 Jun 2020 18:36:51: 11000000 INFO @ Sun, 21 Jun 2020 18:36:53: 16000000 INFO @ Sun, 21 Jun 2020 18:36:56: 7000000 INFO @ Sun, 21 Jun 2020 18:36:58: 12000000 INFO @ Sun, 21 Jun 2020 18:37:00: 17000000 INFO @ Sun, 21 Jun 2020 18:37:01: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 18:37:01: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 18:37:01: #1 total tags in treatment: 17115690 INFO @ Sun, 21 Jun 2020 18:37:01: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:37:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:37:02: #1 tags after filtering in treatment: 17115627 INFO @ Sun, 21 Jun 2020 18:37:02: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:37:02: #1 finished! INFO @ Sun, 21 Jun 2020 18:37:02: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:37:02: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:37:03: 8000000 INFO @ Sun, 21 Jun 2020 18:37:03: #2 number of paired peaks: 68 WARNING @ Sun, 21 Jun 2020 18:37:03: Too few paired peaks (68) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:37:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX2520650/SRX2520650.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2520650/SRX2520650.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2520650/SRX2520650.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2520650/SRX2520650.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 18:37:04: 13000000 INFO @ Sun, 21 Jun 2020 18:37:09: 9000000 INFO @ Sun, 21 Jun 2020 18:37:12: 14000000 INFO @ Sun, 21 Jun 2020 18:37:16: 10000000 INFO @ Sun, 21 Jun 2020 18:37:18: 15000000 INFO @ Sun, 21 Jun 2020 18:37:22: 11000000 INFO @ Sun, 21 Jun 2020 18:37:25: 16000000 INFO @ Sun, 21 Jun 2020 18:37:29: 12000000 INFO @ Sun, 21 Jun 2020 18:37:32: 17000000 INFO @ Sun, 21 Jun 2020 18:37:33: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 18:37:33: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 18:37:33: #1 total tags in treatment: 17115690 INFO @ Sun, 21 Jun 2020 18:37:33: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:37:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:37:33: #1 tags after filtering in treatment: 17115627 INFO @ Sun, 21 Jun 2020 18:37:33: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:37:33: #1 finished! INFO @ Sun, 21 Jun 2020 18:37:33: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:37:33: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:37:35: #2 number of paired peaks: 68 WARNING @ Sun, 21 Jun 2020 18:37:35: Too few paired peaks (68) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:37:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX2520650/SRX2520650.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2520650/SRX2520650.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2520650/SRX2520650.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2520650/SRX2520650.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 18:37:35: 13000000 INFO @ Sun, 21 Jun 2020 18:37:42: 14000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 18:37:48: 15000000 INFO @ Sun, 21 Jun 2020 18:37:55: 16000000 INFO @ Sun, 21 Jun 2020 18:38:02: 17000000 INFO @ Sun, 21 Jun 2020 18:38:02: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 18:38:02: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 18:38:02: #1 total tags in treatment: 17115690 INFO @ Sun, 21 Jun 2020 18:38:02: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:38:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:38:03: #1 tags after filtering in treatment: 17115627 INFO @ Sun, 21 Jun 2020 18:38:03: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:38:03: #1 finished! INFO @ Sun, 21 Jun 2020 18:38:03: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:38:03: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:38:04: #2 number of paired peaks: 68 WARNING @ Sun, 21 Jun 2020 18:38:04: Too few paired peaks (68) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:38:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX2520650/SRX2520650.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2520650/SRX2520650.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2520650/SRX2520650.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2520650/SRX2520650.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。