Job ID = 6454597 SRX = SRX2385060 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T09:25:59 prefetch.2.10.7: 1) Downloading 'SRR5064691'... 2020-06-21T09:25:59 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T09:31:37 prefetch.2.10.7: HTTPS download succeed 2020-06-21T09:31:39 prefetch.2.10.7: 'SRR5064691' is valid 2020-06-21T09:31:39 prefetch.2.10.7: 1) 'SRR5064691' was downloaded successfully 2020-06-21T09:31:39 prefetch.2.10.7: 'SRR5064691' has 0 unresolved dependencies Read 28239481 spots for SRR5064691/SRR5064691.sra Written 28239481 spots for SRR5064691/SRR5064691.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:14 28239481 reads; of these: 28239481 (100.00%) were unpaired; of these: 1377361 (4.88%) aligned 0 times 19603933 (69.42%) aligned exactly 1 time 7258187 (25.70%) aligned >1 times 95.12% overall alignment rate Time searching: 00:08:14 Overall time: 00:08:14 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 7581888 / 26862120 = 0.2823 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:46:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX2385060/SRX2385060.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX2385060/SRX2385060.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX2385060/SRX2385060.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX2385060/SRX2385060.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:46:42: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:46:42: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:46:48: 1000000 INFO @ Sun, 21 Jun 2020 18:46:54: 2000000 INFO @ Sun, 21 Jun 2020 18:46:59: 3000000 INFO @ Sun, 21 Jun 2020 18:47:05: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:47:10: 5000000 INFO @ Sun, 21 Jun 2020 18:47:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX2385060/SRX2385060.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX2385060/SRX2385060.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX2385060/SRX2385060.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX2385060/SRX2385060.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:47:12: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:47:12: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:47:16: 6000000 INFO @ Sun, 21 Jun 2020 18:47:18: 1000000 INFO @ Sun, 21 Jun 2020 18:47:22: 7000000 INFO @ Sun, 21 Jun 2020 18:47:24: 2000000 INFO @ Sun, 21 Jun 2020 18:47:28: 8000000 INFO @ Sun, 21 Jun 2020 18:47:30: 3000000 INFO @ Sun, 21 Jun 2020 18:47:35: 9000000 INFO @ Sun, 21 Jun 2020 18:47:36: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:47:41: 10000000 INFO @ Sun, 21 Jun 2020 18:47:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX2385060/SRX2385060.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX2385060/SRX2385060.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX2385060/SRX2385060.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX2385060/SRX2385060.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:47:42: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:47:42: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:47:42: 5000000 INFO @ Sun, 21 Jun 2020 18:47:47: 11000000 INFO @ Sun, 21 Jun 2020 18:47:48: 1000000 INFO @ Sun, 21 Jun 2020 18:47:48: 6000000 INFO @ Sun, 21 Jun 2020 18:47:53: 12000000 INFO @ Sun, 21 Jun 2020 18:47:54: 2000000 INFO @ Sun, 21 Jun 2020 18:47:54: 7000000 INFO @ Sun, 21 Jun 2020 18:47:59: 13000000 INFO @ Sun, 21 Jun 2020 18:47:59: 3000000 INFO @ Sun, 21 Jun 2020 18:48:00: 8000000 INFO @ Sun, 21 Jun 2020 18:48:05: 14000000 INFO @ Sun, 21 Jun 2020 18:48:05: 4000000 INFO @ Sun, 21 Jun 2020 18:48:06: 9000000 INFO @ Sun, 21 Jun 2020 18:48:11: 15000000 INFO @ Sun, 21 Jun 2020 18:48:11: 5000000 INFO @ Sun, 21 Jun 2020 18:48:12: 10000000 INFO @ Sun, 21 Jun 2020 18:48:17: 6000000 INFO @ Sun, 21 Jun 2020 18:48:18: 16000000 INFO @ Sun, 21 Jun 2020 18:48:18: 11000000 INFO @ Sun, 21 Jun 2020 18:48:23: 7000000 INFO @ Sun, 21 Jun 2020 18:48:24: 17000000 INFO @ Sun, 21 Jun 2020 18:48:25: 12000000 INFO @ Sun, 21 Jun 2020 18:48:29: 8000000 INFO @ Sun, 21 Jun 2020 18:48:31: 18000000 INFO @ Sun, 21 Jun 2020 18:48:31: 13000000 INFO @ Sun, 21 Jun 2020 18:48:35: 9000000 INFO @ Sun, 21 Jun 2020 18:48:37: 14000000 INFO @ Sun, 21 Jun 2020 18:48:37: 19000000 INFO @ Sun, 21 Jun 2020 18:48:39: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 18:48:39: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 18:48:39: #1 total tags in treatment: 19280232 INFO @ Sun, 21 Jun 2020 18:48:39: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:48:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:48:40: #1 tags after filtering in treatment: 19280232 INFO @ Sun, 21 Jun 2020 18:48:40: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:48:40: #1 finished! INFO @ Sun, 21 Jun 2020 18:48:40: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:48:40: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:48:41: #2 number of paired peaks: 92 WARNING @ Sun, 21 Jun 2020 18:48:41: Too few paired peaks (92) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:48:41: Process for pairing-model is terminated! INFO @ Sun, 21 Jun 2020 18:48:41: 10000000 cut: /home/okishinya/chipatlas/results/dm6/SRX2385060/SRX2385060.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2385060/SRX2385060.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2385060/SRX2385060.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2385060/SRX2385060.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 18:48:43: 15000000 INFO @ Sun, 21 Jun 2020 18:48:47: 11000000 INFO @ Sun, 21 Jun 2020 18:48:51: 16000000 INFO @ Sun, 21 Jun 2020 18:48:53: 12000000 INFO @ Sun, 21 Jun 2020 18:48:57: 17000000 INFO @ Sun, 21 Jun 2020 18:48:59: 13000000 INFO @ Sun, 21 Jun 2020 18:49:04: 18000000 INFO @ Sun, 21 Jun 2020 18:49:05: 14000000 INFO @ Sun, 21 Jun 2020 18:49:10: 19000000 INFO @ Sun, 21 Jun 2020 18:49:11: 15000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 18:49:12: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 18:49:12: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 18:49:12: #1 total tags in treatment: 19280232 INFO @ Sun, 21 Jun 2020 18:49:12: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:49:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:49:13: #1 tags after filtering in treatment: 19280232 INFO @ Sun, 21 Jun 2020 18:49:13: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:49:13: #1 finished! INFO @ Sun, 21 Jun 2020 18:49:13: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:49:13: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:49:14: #2 number of paired peaks: 92 WARNING @ Sun, 21 Jun 2020 18:49:14: Too few paired peaks (92) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:49:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX2385060/SRX2385060.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2385060/SRX2385060.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2385060/SRX2385060.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2385060/SRX2385060.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 18:49:18: 16000000 INFO @ Sun, 21 Jun 2020 18:49:23: 17000000 INFO @ Sun, 21 Jun 2020 18:49:29: 18000000 INFO @ Sun, 21 Jun 2020 18:49:35: 19000000 INFO @ Sun, 21 Jun 2020 18:49:37: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 18:49:37: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 18:49:37: #1 total tags in treatment: 19280232 INFO @ Sun, 21 Jun 2020 18:49:37: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:49:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:49:38: #1 tags after filtering in treatment: 19280232 INFO @ Sun, 21 Jun 2020 18:49:38: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:49:38: #1 finished! INFO @ Sun, 21 Jun 2020 18:49:38: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:49:38: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:49:39: #2 number of paired peaks: 92 WARNING @ Sun, 21 Jun 2020 18:49:39: Too few paired peaks (92) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:49:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX2385060/SRX2385060.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2385060/SRX2385060.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2385060/SRX2385060.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2385060/SRX2385060.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。