Job ID = 6454593 SRX = SRX2325664 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T09:15:39 prefetch.2.10.7: 1) Downloading 'SRR4896352'... 2020-06-21T09:15:39 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T09:32:51 prefetch.2.10.7: HTTPS download succeed 2020-06-21T09:32:51 prefetch.2.10.7: 1) 'SRR4896352' was downloaded successfully 2020-06-21T09:32:51 prefetch.2.10.7: 'SRR4896352' has 0 unresolved dependencies Read 118861330 spots for SRR4896352/SRR4896352.sra Written 118861330 spots for SRR4896352/SRR4896352.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:45:05 118861330 reads; of these: 118861330 (100.00%) were unpaired; of these: 9126135 (7.68%) aligned 0 times 87817790 (73.88%) aligned exactly 1 time 21917405 (18.44%) aligned >1 times 92.32% overall alignment rate Time searching: 00:45:05 Overall time: 00:45:05 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 48 files... [bam_rmdupse_core] 60090163 / 109735195 = 0.5476 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:46:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX2325664/SRX2325664.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX2325664/SRX2325664.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX2325664/SRX2325664.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX2325664/SRX2325664.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:46:33: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:46:33: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:46:41: 1000000 INFO @ Sun, 21 Jun 2020 19:46:48: 2000000 INFO @ Sun, 21 Jun 2020 19:46:55: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:47:02: 4000000 INFO @ Sun, 21 Jun 2020 19:47:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX2325664/SRX2325664.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX2325664/SRX2325664.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX2325664/SRX2325664.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX2325664/SRX2325664.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:47:03: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:47:03: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:47:10: 5000000 INFO @ Sun, 21 Jun 2020 19:47:11: 1000000 INFO @ Sun, 21 Jun 2020 19:47:17: 6000000 INFO @ Sun, 21 Jun 2020 19:47:19: 2000000 INFO @ Sun, 21 Jun 2020 19:47:25: 7000000 INFO @ Sun, 21 Jun 2020 19:47:27: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:47:33: 8000000 INFO @ Sun, 21 Jun 2020 19:47:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX2325664/SRX2325664.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX2325664/SRX2325664.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX2325664/SRX2325664.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX2325664/SRX2325664.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:47:33: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:47:33: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:47:35: 4000000 INFO @ Sun, 21 Jun 2020 19:47:41: 9000000 INFO @ Sun, 21 Jun 2020 19:47:42: 1000000 INFO @ Sun, 21 Jun 2020 19:47:43: 5000000 INFO @ Sun, 21 Jun 2020 19:47:49: 10000000 INFO @ Sun, 21 Jun 2020 19:47:49: 2000000 INFO @ Sun, 21 Jun 2020 19:47:50: 6000000 INFO @ Sun, 21 Jun 2020 19:47:56: 11000000 INFO @ Sun, 21 Jun 2020 19:47:57: 3000000 INFO @ Sun, 21 Jun 2020 19:47:59: 7000000 INFO @ Sun, 21 Jun 2020 19:48:04: 12000000 INFO @ Sun, 21 Jun 2020 19:48:05: 4000000 INFO @ Sun, 21 Jun 2020 19:48:06: 8000000 INFO @ Sun, 21 Jun 2020 19:48:12: 13000000 INFO @ Sun, 21 Jun 2020 19:48:13: 5000000 INFO @ Sun, 21 Jun 2020 19:48:14: 9000000 INFO @ Sun, 21 Jun 2020 19:48:20: 14000000 INFO @ Sun, 21 Jun 2020 19:48:21: 6000000 INFO @ Sun, 21 Jun 2020 19:48:22: 10000000 INFO @ Sun, 21 Jun 2020 19:48:27: 15000000 INFO @ Sun, 21 Jun 2020 19:48:29: 7000000 INFO @ Sun, 21 Jun 2020 19:48:30: 11000000 INFO @ Sun, 21 Jun 2020 19:48:35: 16000000 INFO @ Sun, 21 Jun 2020 19:48:37: 8000000 INFO @ Sun, 21 Jun 2020 19:48:38: 12000000 INFO @ Sun, 21 Jun 2020 19:48:43: 17000000 INFO @ Sun, 21 Jun 2020 19:48:44: 9000000 INFO @ Sun, 21 Jun 2020 19:48:46: 13000000 INFO @ Sun, 21 Jun 2020 19:48:51: 18000000 INFO @ Sun, 21 Jun 2020 19:48:52: 10000000 INFO @ Sun, 21 Jun 2020 19:48:54: 14000000 INFO @ Sun, 21 Jun 2020 19:48:59: 19000000 INFO @ Sun, 21 Jun 2020 19:49:00: 11000000 INFO @ Sun, 21 Jun 2020 19:49:02: 15000000 INFO @ Sun, 21 Jun 2020 19:49:07: 20000000 INFO @ Sun, 21 Jun 2020 19:49:08: 12000000 INFO @ Sun, 21 Jun 2020 19:49:09: 16000000 INFO @ Sun, 21 Jun 2020 19:49:14: 21000000 INFO @ Sun, 21 Jun 2020 19:49:16: 13000000 INFO @ Sun, 21 Jun 2020 19:49:17: 17000000 INFO @ Sun, 21 Jun 2020 19:49:22: 22000000 INFO @ Sun, 21 Jun 2020 19:49:24: 14000000 INFO @ Sun, 21 Jun 2020 19:49:25: 18000000 INFO @ Sun, 21 Jun 2020 19:49:30: 23000000 INFO @ Sun, 21 Jun 2020 19:49:32: 15000000 INFO @ Sun, 21 Jun 2020 19:49:32: 19000000 INFO @ Sun, 21 Jun 2020 19:49:38: 24000000 INFO @ Sun, 21 Jun 2020 19:49:39: 16000000 INFO @ Sun, 21 Jun 2020 19:49:40: 20000000 INFO @ Sun, 21 Jun 2020 19:49:45: 25000000 INFO @ Sun, 21 Jun 2020 19:49:47: 17000000 INFO @ Sun, 21 Jun 2020 19:49:49: 21000000 INFO @ Sun, 21 Jun 2020 19:49:53: 26000000 INFO @ Sun, 21 Jun 2020 19:49:55: 18000000 INFO @ Sun, 21 Jun 2020 19:49:56: 22000000 INFO @ Sun, 21 Jun 2020 19:50:01: 27000000 INFO @ Sun, 21 Jun 2020 19:50:03: 19000000 INFO @ Sun, 21 Jun 2020 19:50:04: 23000000 INFO @ Sun, 21 Jun 2020 19:50:09: 28000000 INFO @ Sun, 21 Jun 2020 19:50:11: 20000000 INFO @ Sun, 21 Jun 2020 19:50:12: 24000000 INFO @ Sun, 21 Jun 2020 19:50:17: 29000000 INFO @ Sun, 21 Jun 2020 19:50:19: 21000000 INFO @ Sun, 21 Jun 2020 19:50:20: 25000000 INFO @ Sun, 21 Jun 2020 19:50:24: 30000000 INFO @ Sun, 21 Jun 2020 19:50:26: 22000000 INFO @ Sun, 21 Jun 2020 19:50:28: 26000000 INFO @ Sun, 21 Jun 2020 19:50:32: 31000000 INFO @ Sun, 21 Jun 2020 19:50:34: 23000000 INFO @ Sun, 21 Jun 2020 19:50:36: 27000000 INFO @ Sun, 21 Jun 2020 19:50:40: 32000000 INFO @ Sun, 21 Jun 2020 19:50:42: 24000000 INFO @ Sun, 21 Jun 2020 19:50:44: 28000000 INFO @ Sun, 21 Jun 2020 19:50:47: 33000000 INFO @ Sun, 21 Jun 2020 19:50:50: 25000000 INFO @ Sun, 21 Jun 2020 19:50:52: 29000000 INFO @ Sun, 21 Jun 2020 19:50:55: 34000000 INFO @ Sun, 21 Jun 2020 19:50:58: 26000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 19:50:59: 30000000 INFO @ Sun, 21 Jun 2020 19:51:03: 35000000 INFO @ Sun, 21 Jun 2020 19:51:05: 27000000 INFO @ Sun, 21 Jun 2020 19:51:07: 31000000 INFO @ Sun, 21 Jun 2020 19:51:11: 36000000 INFO @ Sun, 21 Jun 2020 19:51:13: 28000000 INFO @ Sun, 21 Jun 2020 19:51:15: 32000000 INFO @ Sun, 21 Jun 2020 19:51:18: 37000000 INFO @ Sun, 21 Jun 2020 19:51:21: 29000000 INFO @ Sun, 21 Jun 2020 19:51:23: 33000000 INFO @ Sun, 21 Jun 2020 19:51:26: 38000000 INFO @ Sun, 21 Jun 2020 19:51:29: 30000000 INFO @ Sun, 21 Jun 2020 19:51:31: 34000000 INFO @ Sun, 21 Jun 2020 19:51:34: 39000000 INFO @ Sun, 21 Jun 2020 19:51:37: 31000000 INFO @ Sun, 21 Jun 2020 19:51:39: 35000000 INFO @ Sun, 21 Jun 2020 19:51:42: 40000000 INFO @ Sun, 21 Jun 2020 19:51:45: 32000000 INFO @ Sun, 21 Jun 2020 19:51:47: 36000000 INFO @ Sun, 21 Jun 2020 19:51:49: 41000000 INFO @ Sun, 21 Jun 2020 19:51:53: 33000000 INFO @ Sun, 21 Jun 2020 19:51:54: 37000000 INFO @ Sun, 21 Jun 2020 19:51:57: 42000000 INFO @ Sun, 21 Jun 2020 19:52:01: 34000000 INFO @ Sun, 21 Jun 2020 19:52:02: 38000000 INFO @ Sun, 21 Jun 2020 19:52:05: 43000000 INFO @ Sun, 21 Jun 2020 19:52:09: 35000000 INFO @ Sun, 21 Jun 2020 19:52:10: 39000000 INFO @ Sun, 21 Jun 2020 19:52:13: 44000000 INFO @ Sun, 21 Jun 2020 19:52:17: 36000000 INFO @ Sun, 21 Jun 2020 19:52:18: 40000000 INFO @ Sun, 21 Jun 2020 19:52:21: 45000000 BigWig に変換しました。 INFO @ Sun, 21 Jun 2020 19:52:24: 37000000 INFO @ Sun, 21 Jun 2020 19:52:25: 41000000 INFO @ Sun, 21 Jun 2020 19:52:29: 46000000 INFO @ Sun, 21 Jun 2020 19:52:32: 38000000 INFO @ Sun, 21 Jun 2020 19:52:33: 42000000 INFO @ Sun, 21 Jun 2020 19:52:36: 47000000 INFO @ Sun, 21 Jun 2020 19:52:40: 39000000 INFO @ Sun, 21 Jun 2020 19:52:41: 43000000 INFO @ Sun, 21 Jun 2020 19:52:44: 48000000 INFO @ Sun, 21 Jun 2020 19:52:48: 40000000 INFO @ Sun, 21 Jun 2020 19:52:49: 44000000 INFO @ Sun, 21 Jun 2020 19:52:52: 49000000 INFO @ Sun, 21 Jun 2020 19:52:56: 41000000 INFO @ Sun, 21 Jun 2020 19:52:57: 45000000 INFO @ Sun, 21 Jun 2020 19:52:58: #1 tag size is determined as 76 bps INFO @ Sun, 21 Jun 2020 19:52:58: #1 tag size = 76 INFO @ Sun, 21 Jun 2020 19:52:58: #1 total tags in treatment: 49645032 INFO @ Sun, 21 Jun 2020 19:52:58: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:52:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:52:59: #1 tags after filtering in treatment: 49645030 INFO @ Sun, 21 Jun 2020 19:52:59: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:52:59: #1 finished! INFO @ Sun, 21 Jun 2020 19:52:59: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:52:59: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:53:02: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 19:53:02: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:53:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX2325664/SRX2325664.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2325664/SRX2325664.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2325664/SRX2325664.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2325664/SRX2325664.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 19:53:04: 42000000 INFO @ Sun, 21 Jun 2020 19:53:05: 46000000 INFO @ Sun, 21 Jun 2020 19:53:12: 43000000 INFO @ Sun, 21 Jun 2020 19:53:13: 47000000 INFO @ Sun, 21 Jun 2020 19:53:20: 44000000 INFO @ Sun, 21 Jun 2020 19:53:20: 48000000 INFO @ Sun, 21 Jun 2020 19:53:28: 45000000 INFO @ Sun, 21 Jun 2020 19:53:28: 49000000 INFO @ Sun, 21 Jun 2020 19:53:34: #1 tag size is determined as 76 bps INFO @ Sun, 21 Jun 2020 19:53:34: #1 tag size = 76 INFO @ Sun, 21 Jun 2020 19:53:34: #1 total tags in treatment: 49645032 INFO @ Sun, 21 Jun 2020 19:53:34: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:53:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:53:35: #1 tags after filtering in treatment: 49645030 INFO @ Sun, 21 Jun 2020 19:53:35: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:53:35: #1 finished! INFO @ Sun, 21 Jun 2020 19:53:35: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:53:35: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:53:36: 46000000 INFO @ Sun, 21 Jun 2020 19:53:38: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 19:53:38: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:53:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX2325664/SRX2325664.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2325664/SRX2325664.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2325664/SRX2325664.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2325664/SRX2325664.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 19:53:44: 47000000 INFO @ Sun, 21 Jun 2020 19:53:51: 48000000 INFO @ Sun, 21 Jun 2020 19:53:58: 49000000 INFO @ Sun, 21 Jun 2020 19:54:03: #1 tag size is determined as 76 bps INFO @ Sun, 21 Jun 2020 19:54:03: #1 tag size = 76 INFO @ Sun, 21 Jun 2020 19:54:03: #1 total tags in treatment: 49645032 INFO @ Sun, 21 Jun 2020 19:54:03: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:54:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:54:05: #1 tags after filtering in treatment: 49645030 INFO @ Sun, 21 Jun 2020 19:54:05: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:54:05: #1 finished! INFO @ Sun, 21 Jun 2020 19:54:05: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:54:05: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:54:08: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 19:54:08: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:54:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX2325664/SRX2325664.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2325664/SRX2325664.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2325664/SRX2325664.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2325664/SRX2325664.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling