Job ID = 6454586 SRX = SRX2325659 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T09:13:39 prefetch.2.10.7: 1) Downloading 'SRR4896347'... 2020-06-21T09:13:39 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T09:23:25 prefetch.2.10.7: HTTPS download succeed 2020-06-21T09:23:25 prefetch.2.10.7: 1) 'SRR4896347' was downloaded successfully 2020-06-21T09:23:25 prefetch.2.10.7: 'SRR4896347' has 0 unresolved dependencies Read 66927365 spots for SRR4896347/SRR4896347.sra Written 66927365 spots for SRR4896347/SRR4896347.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:21:48 66927365 reads; of these: 66927365 (100.00%) were unpaired; of these: 16289382 (24.34%) aligned 0 times 40888109 (61.09%) aligned exactly 1 time 9749874 (14.57%) aligned >1 times 75.66% overall alignment rate Time searching: 00:21:48 Overall time: 00:21:48 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 19713800 / 50637983 = 0.3893 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:59:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX2325659/SRX2325659.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX2325659/SRX2325659.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX2325659/SRX2325659.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX2325659/SRX2325659.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:59:27: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:59:27: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:59:32: 1000000 INFO @ Sun, 21 Jun 2020 18:59:37: 2000000 INFO @ Sun, 21 Jun 2020 18:59:43: 3000000 INFO @ Sun, 21 Jun 2020 18:59:48: 4000000 INFO @ Sun, 21 Jun 2020 18:59:53: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:59:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX2325659/SRX2325659.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX2325659/SRX2325659.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX2325659/SRX2325659.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX2325659/SRX2325659.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:59:57: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:59:57: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:59:59: 6000000 INFO @ Sun, 21 Jun 2020 19:00:03: 1000000 INFO @ Sun, 21 Jun 2020 19:00:05: 7000000 INFO @ Sun, 21 Jun 2020 19:00:10: 2000000 INFO @ Sun, 21 Jun 2020 19:00:11: 8000000 INFO @ Sun, 21 Jun 2020 19:00:16: 9000000 INFO @ Sun, 21 Jun 2020 19:00:17: 3000000 INFO @ Sun, 21 Jun 2020 19:00:22: 10000000 INFO @ Sun, 21 Jun 2020 19:00:23: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:00:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX2325659/SRX2325659.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX2325659/SRX2325659.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX2325659/SRX2325659.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX2325659/SRX2325659.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:00:27: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:00:27: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:00:28: 11000000 INFO @ Sun, 21 Jun 2020 19:00:30: 5000000 INFO @ Sun, 21 Jun 2020 19:00:33: 1000000 INFO @ Sun, 21 Jun 2020 19:00:34: 12000000 INFO @ Sun, 21 Jun 2020 19:00:36: 6000000 INFO @ Sun, 21 Jun 2020 19:00:39: 2000000 INFO @ Sun, 21 Jun 2020 19:00:40: 13000000 INFO @ Sun, 21 Jun 2020 19:00:43: 7000000 INFO @ Sun, 21 Jun 2020 19:00:45: 3000000 INFO @ Sun, 21 Jun 2020 19:00:46: 14000000 INFO @ Sun, 21 Jun 2020 19:00:50: 8000000 INFO @ Sun, 21 Jun 2020 19:00:50: 4000000 INFO @ Sun, 21 Jun 2020 19:00:52: 15000000 INFO @ Sun, 21 Jun 2020 19:00:57: 5000000 INFO @ Sun, 21 Jun 2020 19:00:57: 9000000 INFO @ Sun, 21 Jun 2020 19:00:58: 16000000 INFO @ Sun, 21 Jun 2020 19:01:02: 6000000 INFO @ Sun, 21 Jun 2020 19:01:03: 17000000 INFO @ Sun, 21 Jun 2020 19:01:04: 10000000 INFO @ Sun, 21 Jun 2020 19:01:08: 7000000 INFO @ Sun, 21 Jun 2020 19:01:09: 18000000 INFO @ Sun, 21 Jun 2020 19:01:10: 11000000 INFO @ Sun, 21 Jun 2020 19:01:14: 8000000 INFO @ Sun, 21 Jun 2020 19:01:15: 19000000 INFO @ Sun, 21 Jun 2020 19:01:17: 12000000 INFO @ Sun, 21 Jun 2020 19:01:20: 9000000 INFO @ Sun, 21 Jun 2020 19:01:21: 20000000 INFO @ Sun, 21 Jun 2020 19:01:24: 13000000 INFO @ Sun, 21 Jun 2020 19:01:26: 10000000 INFO @ Sun, 21 Jun 2020 19:01:26: 21000000 INFO @ Sun, 21 Jun 2020 19:01:30: 14000000 INFO @ Sun, 21 Jun 2020 19:01:32: 11000000 INFO @ Sun, 21 Jun 2020 19:01:32: 22000000 INFO @ Sun, 21 Jun 2020 19:01:37: 15000000 INFO @ Sun, 21 Jun 2020 19:01:38: 12000000 INFO @ Sun, 21 Jun 2020 19:01:38: 23000000 INFO @ Sun, 21 Jun 2020 19:01:43: 16000000 INFO @ Sun, 21 Jun 2020 19:01:44: 13000000 INFO @ Sun, 21 Jun 2020 19:01:44: 24000000 INFO @ Sun, 21 Jun 2020 19:01:49: 14000000 INFO @ Sun, 21 Jun 2020 19:01:50: 25000000 INFO @ Sun, 21 Jun 2020 19:01:50: 17000000 INFO @ Sun, 21 Jun 2020 19:01:55: 15000000 INFO @ Sun, 21 Jun 2020 19:01:55: 26000000 INFO @ Sun, 21 Jun 2020 19:01:56: 18000000 INFO @ Sun, 21 Jun 2020 19:02:01: 16000000 INFO @ Sun, 21 Jun 2020 19:02:02: 27000000 INFO @ Sun, 21 Jun 2020 19:02:03: 19000000 INFO @ Sun, 21 Jun 2020 19:02:07: 17000000 INFO @ Sun, 21 Jun 2020 19:02:07: 28000000 INFO @ Sun, 21 Jun 2020 19:02:09: 20000000 INFO @ Sun, 21 Jun 2020 19:02:13: 18000000 INFO @ Sun, 21 Jun 2020 19:02:13: 29000000 INFO @ Sun, 21 Jun 2020 19:02:16: 21000000 INFO @ Sun, 21 Jun 2020 19:02:18: 19000000 INFO @ Sun, 21 Jun 2020 19:02:19: 30000000 INFO @ Sun, 21 Jun 2020 19:02:22: 22000000 INFO @ Sun, 21 Jun 2020 19:02:24: 20000000 INFO @ Sun, 21 Jun 2020 19:02:25: #1 tag size is determined as 76 bps INFO @ Sun, 21 Jun 2020 19:02:25: #1 tag size = 76 INFO @ Sun, 21 Jun 2020 19:02:25: #1 total tags in treatment: 30924183 INFO @ Sun, 21 Jun 2020 19:02:25: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:02:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:02:26: #1 tags after filtering in treatment: 30924181 INFO @ Sun, 21 Jun 2020 19:02:26: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:02:26: #1 finished! INFO @ Sun, 21 Jun 2020 19:02:26: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:02:26: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:02:28: #2 number of paired peaks: 93 WARNING @ Sun, 21 Jun 2020 19:02:28: Too few paired peaks (93) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:02:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX2325659/SRX2325659.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2325659/SRX2325659.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2325659/SRX2325659.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2325659/SRX2325659.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 19:02:29: 23000000 INFO @ Sun, 21 Jun 2020 19:02:30: 21000000 INFO @ Sun, 21 Jun 2020 19:02:35: 24000000 INFO @ Sun, 21 Jun 2020 19:02:35: 22000000 INFO @ Sun, 21 Jun 2020 19:02:41: 23000000 INFO @ Sun, 21 Jun 2020 19:02:42: 25000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 19:02:47: 24000000 INFO @ Sun, 21 Jun 2020 19:02:48: 26000000 INFO @ Sun, 21 Jun 2020 19:02:53: 25000000 INFO @ Sun, 21 Jun 2020 19:02:55: 27000000 INFO @ Sun, 21 Jun 2020 19:02:59: 26000000 INFO @ Sun, 21 Jun 2020 19:03:02: 28000000 INFO @ Sun, 21 Jun 2020 19:03:05: 27000000 INFO @ Sun, 21 Jun 2020 19:03:09: 29000000 INFO @ Sun, 21 Jun 2020 19:03:11: 28000000 INFO @ Sun, 21 Jun 2020 19:03:16: 30000000 INFO @ Sun, 21 Jun 2020 19:03:17: 29000000 INFO @ Sun, 21 Jun 2020 19:03:22: 30000000 INFO @ Sun, 21 Jun 2020 19:03:22: #1 tag size is determined as 76 bps INFO @ Sun, 21 Jun 2020 19:03:22: #1 tag size = 76 INFO @ Sun, 21 Jun 2020 19:03:22: #1 total tags in treatment: 30924183 INFO @ Sun, 21 Jun 2020 19:03:22: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:03:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:03:23: #1 tags after filtering in treatment: 30924181 INFO @ Sun, 21 Jun 2020 19:03:23: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:03:23: #1 finished! INFO @ Sun, 21 Jun 2020 19:03:23: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:03:23: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:03:25: #2 number of paired peaks: 93 WARNING @ Sun, 21 Jun 2020 19:03:25: Too few paired peaks (93) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:03:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX2325659/SRX2325659.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2325659/SRX2325659.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2325659/SRX2325659.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2325659/SRX2325659.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 19:03:28: #1 tag size is determined as 76 bps INFO @ Sun, 21 Jun 2020 19:03:28: #1 tag size = 76 INFO @ Sun, 21 Jun 2020 19:03:28: #1 total tags in treatment: 30924183 INFO @ Sun, 21 Jun 2020 19:03:28: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:03:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:03:29: #1 tags after filtering in treatment: 30924181 INFO @ Sun, 21 Jun 2020 19:03:29: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:03:29: #1 finished! INFO @ Sun, 21 Jun 2020 19:03:29: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:03:29: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:03:31: #2 number of paired peaks: 93 WARNING @ Sun, 21 Jun 2020 19:03:31: Too few paired peaks (93) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:03:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX2325659/SRX2325659.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2325659/SRX2325659.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2325659/SRX2325659.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2325659/SRX2325659.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。