Job ID = 6454584 SRX = SRX2325657 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T09:28:14 prefetch.2.10.7: 1) Downloading 'SRR4896345'... 2020-06-21T09:28:14 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T09:37:14 prefetch.2.10.7: HTTPS download succeed 2020-06-21T09:37:14 prefetch.2.10.7: 1) 'SRR4896345' was downloaded successfully 2020-06-21T09:37:14 prefetch.2.10.7: 'SRR4896345' has 0 unresolved dependencies Read 57994697 spots for SRR4896345/SRR4896345.sra Written 57994697 spots for SRR4896345/SRR4896345.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:21:16 57994697 reads; of these: 57994697 (100.00%) were unpaired; of these: 3166253 (5.46%) aligned 0 times 44395908 (76.55%) aligned exactly 1 time 10432536 (17.99%) aligned >1 times 94.54% overall alignment rate Time searching: 00:21:16 Overall time: 00:21:16 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 11790568 / 54828444 = 0.2150 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:13:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX2325657/SRX2325657.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX2325657/SRX2325657.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX2325657/SRX2325657.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX2325657/SRX2325657.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:13:42: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:13:42: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:13:48: 1000000 INFO @ Sun, 21 Jun 2020 19:13:53: 2000000 INFO @ Sun, 21 Jun 2020 19:13:59: 3000000 INFO @ Sun, 21 Jun 2020 19:14:04: 4000000 INFO @ Sun, 21 Jun 2020 19:14:09: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:14:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX2325657/SRX2325657.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX2325657/SRX2325657.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX2325657/SRX2325657.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX2325657/SRX2325657.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:14:12: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:14:12: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:14:15: 6000000 INFO @ Sun, 21 Jun 2020 19:14:18: 1000000 INFO @ Sun, 21 Jun 2020 19:14:21: 7000000 INFO @ Sun, 21 Jun 2020 19:14:24: 2000000 INFO @ Sun, 21 Jun 2020 19:14:27: 8000000 INFO @ Sun, 21 Jun 2020 19:14:30: 3000000 INFO @ Sun, 21 Jun 2020 19:14:33: 9000000 INFO @ Sun, 21 Jun 2020 19:14:36: 4000000 INFO @ Sun, 21 Jun 2020 19:14:38: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:14:42: 5000000 INFO @ Sun, 21 Jun 2020 19:14:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX2325657/SRX2325657.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX2325657/SRX2325657.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX2325657/SRX2325657.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX2325657/SRX2325657.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:14:42: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:14:42: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:14:44: 11000000 INFO @ Sun, 21 Jun 2020 19:14:48: 6000000 INFO @ Sun, 21 Jun 2020 19:14:48: 1000000 INFO @ Sun, 21 Jun 2020 19:14:50: 12000000 INFO @ Sun, 21 Jun 2020 19:14:53: 7000000 INFO @ Sun, 21 Jun 2020 19:14:54: 2000000 INFO @ Sun, 21 Jun 2020 19:14:56: 13000000 INFO @ Sun, 21 Jun 2020 19:14:59: 8000000 INFO @ Sun, 21 Jun 2020 19:15:00: 3000000 INFO @ Sun, 21 Jun 2020 19:15:02: 14000000 INFO @ Sun, 21 Jun 2020 19:15:05: 9000000 INFO @ Sun, 21 Jun 2020 19:15:06: 4000000 INFO @ Sun, 21 Jun 2020 19:15:08: 15000000 INFO @ Sun, 21 Jun 2020 19:15:11: 10000000 INFO @ Sun, 21 Jun 2020 19:15:12: 5000000 INFO @ Sun, 21 Jun 2020 19:15:14: 16000000 INFO @ Sun, 21 Jun 2020 19:15:17: 11000000 INFO @ Sun, 21 Jun 2020 19:15:18: 6000000 INFO @ Sun, 21 Jun 2020 19:15:20: 17000000 INFO @ Sun, 21 Jun 2020 19:15:23: 12000000 INFO @ Sun, 21 Jun 2020 19:15:24: 7000000 INFO @ Sun, 21 Jun 2020 19:15:26: 18000000 INFO @ Sun, 21 Jun 2020 19:15:29: 13000000 INFO @ Sun, 21 Jun 2020 19:15:30: 8000000 INFO @ Sun, 21 Jun 2020 19:15:32: 19000000 INFO @ Sun, 21 Jun 2020 19:15:35: 14000000 INFO @ Sun, 21 Jun 2020 19:15:36: 9000000 INFO @ Sun, 21 Jun 2020 19:15:38: 20000000 INFO @ Sun, 21 Jun 2020 19:15:41: 15000000 INFO @ Sun, 21 Jun 2020 19:15:43: 10000000 INFO @ Sun, 21 Jun 2020 19:15:44: 21000000 INFO @ Sun, 21 Jun 2020 19:15:47: 16000000 INFO @ Sun, 21 Jun 2020 19:15:49: 11000000 INFO @ Sun, 21 Jun 2020 19:15:50: 22000000 INFO @ Sun, 21 Jun 2020 19:15:53: 17000000 INFO @ Sun, 21 Jun 2020 19:15:55: 12000000 INFO @ Sun, 21 Jun 2020 19:15:56: 23000000 INFO @ Sun, 21 Jun 2020 19:15:59: 18000000 INFO @ Sun, 21 Jun 2020 19:16:01: 13000000 INFO @ Sun, 21 Jun 2020 19:16:02: 24000000 INFO @ Sun, 21 Jun 2020 19:16:05: 19000000 INFO @ Sun, 21 Jun 2020 19:16:07: 14000000 INFO @ Sun, 21 Jun 2020 19:16:08: 25000000 INFO @ Sun, 21 Jun 2020 19:16:11: 20000000 INFO @ Sun, 21 Jun 2020 19:16:13: 15000000 INFO @ Sun, 21 Jun 2020 19:16:14: 26000000 INFO @ Sun, 21 Jun 2020 19:16:18: 21000000 INFO @ Sun, 21 Jun 2020 19:16:19: 16000000 INFO @ Sun, 21 Jun 2020 19:16:20: 27000000 INFO @ Sun, 21 Jun 2020 19:16:24: 22000000 INFO @ Sun, 21 Jun 2020 19:16:25: 17000000 INFO @ Sun, 21 Jun 2020 19:16:26: 28000000 INFO @ Sun, 21 Jun 2020 19:16:30: 23000000 INFO @ Sun, 21 Jun 2020 19:16:31: 18000000 INFO @ Sun, 21 Jun 2020 19:16:32: 29000000 INFO @ Sun, 21 Jun 2020 19:16:36: 24000000 INFO @ Sun, 21 Jun 2020 19:16:37: 19000000 INFO @ Sun, 21 Jun 2020 19:16:38: 30000000 INFO @ Sun, 21 Jun 2020 19:16:42: 25000000 INFO @ Sun, 21 Jun 2020 19:16:43: 20000000 INFO @ Sun, 21 Jun 2020 19:16:44: 31000000 INFO @ Sun, 21 Jun 2020 19:16:48: 26000000 INFO @ Sun, 21 Jun 2020 19:16:50: 21000000 INFO @ Sun, 21 Jun 2020 19:16:50: 32000000 INFO @ Sun, 21 Jun 2020 19:16:54: 27000000 INFO @ Sun, 21 Jun 2020 19:16:56: 22000000 INFO @ Sun, 21 Jun 2020 19:16:56: 33000000 INFO @ Sun, 21 Jun 2020 19:17:00: 28000000 INFO @ Sun, 21 Jun 2020 19:17:02: 23000000 INFO @ Sun, 21 Jun 2020 19:17:03: 34000000 INFO @ Sun, 21 Jun 2020 19:17:06: 29000000 INFO @ Sun, 21 Jun 2020 19:17:08: 24000000 INFO @ Sun, 21 Jun 2020 19:17:09: 35000000 INFO @ Sun, 21 Jun 2020 19:17:12: 30000000 INFO @ Sun, 21 Jun 2020 19:17:14: 25000000 INFO @ Sun, 21 Jun 2020 19:17:15: 36000000 INFO @ Sun, 21 Jun 2020 19:17:18: 31000000 INFO @ Sun, 21 Jun 2020 19:17:20: 26000000 INFO @ Sun, 21 Jun 2020 19:17:21: 37000000 INFO @ Sun, 21 Jun 2020 19:17:24: 32000000 INFO @ Sun, 21 Jun 2020 19:17:26: 27000000 INFO @ Sun, 21 Jun 2020 19:17:27: 38000000 INFO @ Sun, 21 Jun 2020 19:17:30: 33000000 INFO @ Sun, 21 Jun 2020 19:17:32: 28000000 INFO @ Sun, 21 Jun 2020 19:17:33: 39000000 INFO @ Sun, 21 Jun 2020 19:17:36: 34000000 INFO @ Sun, 21 Jun 2020 19:17:38: 29000000 INFO @ Sun, 21 Jun 2020 19:17:39: 40000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 19:17:42: 35000000 INFO @ Sun, 21 Jun 2020 19:17:44: 30000000 INFO @ Sun, 21 Jun 2020 19:17:45: 41000000 INFO @ Sun, 21 Jun 2020 19:17:48: 36000000 INFO @ Sun, 21 Jun 2020 19:17:50: 31000000 INFO @ Sun, 21 Jun 2020 19:17:52: 42000000 INFO @ Sun, 21 Jun 2020 19:17:55: 37000000 INFO @ Sun, 21 Jun 2020 19:17:56: 32000000 INFO @ Sun, 21 Jun 2020 19:17:58: 43000000 INFO @ Sun, 21 Jun 2020 19:17:59: #1 tag size is determined as 76 bps INFO @ Sun, 21 Jun 2020 19:17:59: #1 tag size = 76 INFO @ Sun, 21 Jun 2020 19:17:59: #1 total tags in treatment: 43037876 INFO @ Sun, 21 Jun 2020 19:17:59: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:17:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:17:59: #1 tags after filtering in treatment: 43037875 INFO @ Sun, 21 Jun 2020 19:17:59: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:17:59: #1 finished! INFO @ Sun, 21 Jun 2020 19:17:59: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:17:59: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:18:01: 38000000 INFO @ Sun, 21 Jun 2020 19:18:02: 33000000 INFO @ Sun, 21 Jun 2020 19:18:02: #2 number of paired peaks: 27 WARNING @ Sun, 21 Jun 2020 19:18:02: Too few paired peaks (27) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:18:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX2325657/SRX2325657.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2325657/SRX2325657.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2325657/SRX2325657.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2325657/SRX2325657.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 19:18:07: 39000000 INFO @ Sun, 21 Jun 2020 19:18:08: 34000000 INFO @ Sun, 21 Jun 2020 19:18:13: 40000000 INFO @ Sun, 21 Jun 2020 19:18:14: 35000000 INFO @ Sun, 21 Jun 2020 19:18:19: 41000000 INFO @ Sun, 21 Jun 2020 19:18:21: 36000000 INFO @ Sun, 21 Jun 2020 19:18:25: 42000000 INFO @ Sun, 21 Jun 2020 19:18:27: 37000000 INFO @ Sun, 21 Jun 2020 19:18:32: 43000000 INFO @ Sun, 21 Jun 2020 19:18:32: #1 tag size is determined as 76 bps INFO @ Sun, 21 Jun 2020 19:18:32: #1 tag size = 76 INFO @ Sun, 21 Jun 2020 19:18:32: #1 total tags in treatment: 43037876 INFO @ Sun, 21 Jun 2020 19:18:32: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:18:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:18:33: #1 tags after filtering in treatment: 43037875 INFO @ Sun, 21 Jun 2020 19:18:33: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:18:33: #1 finished! INFO @ Sun, 21 Jun 2020 19:18:33: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:18:33: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:18:33: 38000000 INFO @ Sun, 21 Jun 2020 19:18:36: #2 number of paired peaks: 27 WARNING @ Sun, 21 Jun 2020 19:18:36: Too few paired peaks (27) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:18:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX2325657/SRX2325657.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2325657/SRX2325657.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2325657/SRX2325657.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2325657/SRX2325657.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 19:18:39: 39000000 INFO @ Sun, 21 Jun 2020 19:18:45: 40000000 INFO @ Sun, 21 Jun 2020 19:18:51: 41000000 INFO @ Sun, 21 Jun 2020 19:18:57: 42000000 BigWig に変換しました。 INFO @ Sun, 21 Jun 2020 19:19:03: 43000000 INFO @ Sun, 21 Jun 2020 19:19:04: #1 tag size is determined as 76 bps INFO @ Sun, 21 Jun 2020 19:19:04: #1 tag size = 76 INFO @ Sun, 21 Jun 2020 19:19:04: #1 total tags in treatment: 43037876 INFO @ Sun, 21 Jun 2020 19:19:04: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:19:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:19:05: #1 tags after filtering in treatment: 43037875 INFO @ Sun, 21 Jun 2020 19:19:05: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:19:05: #1 finished! INFO @ Sun, 21 Jun 2020 19:19:05: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:19:05: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:19:07: #2 number of paired peaks: 27 WARNING @ Sun, 21 Jun 2020 19:19:07: Too few paired peaks (27) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:19:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX2325657/SRX2325657.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2325657/SRX2325657.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2325657/SRX2325657.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2325657/SRX2325657.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling