Job ID = 6454582 SRX = SRX2325655 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T09:19:24 prefetch.2.10.7: 1) Downloading 'SRR4896343'... 2020-06-21T09:19:24 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T09:26:41 prefetch.2.10.7: HTTPS download succeed 2020-06-21T09:26:41 prefetch.2.10.7: 1) 'SRR4896343' was downloaded successfully 2020-06-21T09:26:41 prefetch.2.10.7: 'SRR4896343' has 0 unresolved dependencies Read 49026447 spots for SRR4896343/SRR4896343.sra Written 49026447 spots for SRR4896343/SRR4896343.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:16:07 49026447 reads; of these: 49026447 (100.00%) were unpaired; of these: 5561530 (11.34%) aligned 0 times 35434064 (72.28%) aligned exactly 1 time 8030853 (16.38%) aligned >1 times 88.66% overall alignment rate Time searching: 00:16:07 Overall time: 00:16:07 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 8664448 / 43464917 = 0.1993 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:55:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX2325655/SRX2325655.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX2325655/SRX2325655.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX2325655/SRX2325655.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX2325655/SRX2325655.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:55:31: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:55:31: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:55:36: 1000000 INFO @ Sun, 21 Jun 2020 18:55:42: 2000000 INFO @ Sun, 21 Jun 2020 18:55:47: 3000000 INFO @ Sun, 21 Jun 2020 18:55:53: 4000000 INFO @ Sun, 21 Jun 2020 18:55:58: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:56:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX2325655/SRX2325655.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX2325655/SRX2325655.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX2325655/SRX2325655.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX2325655/SRX2325655.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:56:01: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:56:01: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:56:04: 6000000 INFO @ Sun, 21 Jun 2020 18:56:07: 1000000 INFO @ Sun, 21 Jun 2020 18:56:10: 7000000 INFO @ Sun, 21 Jun 2020 18:56:13: 2000000 INFO @ Sun, 21 Jun 2020 18:56:17: 8000000 INFO @ Sun, 21 Jun 2020 18:56:19: 3000000 INFO @ Sun, 21 Jun 2020 18:56:23: 9000000 INFO @ Sun, 21 Jun 2020 18:56:25: 4000000 BedGraph に変換中... INFO @ Sun, 21 Jun 2020 18:56:29: 10000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:56:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX2325655/SRX2325655.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX2325655/SRX2325655.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX2325655/SRX2325655.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX2325655/SRX2325655.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:56:31: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:56:31: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:56:31: 5000000 INFO @ Sun, 21 Jun 2020 18:56:35: 11000000 INFO @ Sun, 21 Jun 2020 18:56:37: 1000000 INFO @ Sun, 21 Jun 2020 18:56:38: 6000000 INFO @ Sun, 21 Jun 2020 18:56:42: 12000000 INFO @ Sun, 21 Jun 2020 18:56:44: 2000000 INFO @ Sun, 21 Jun 2020 18:56:44: 7000000 INFO @ Sun, 21 Jun 2020 18:56:48: 13000000 INFO @ Sun, 21 Jun 2020 18:56:51: 8000000 INFO @ Sun, 21 Jun 2020 18:56:51: 3000000 INFO @ Sun, 21 Jun 2020 18:56:55: 14000000 INFO @ Sun, 21 Jun 2020 18:56:57: 9000000 INFO @ Sun, 21 Jun 2020 18:56:57: 4000000 INFO @ Sun, 21 Jun 2020 18:57:01: 15000000 INFO @ Sun, 21 Jun 2020 18:57:03: 10000000 INFO @ Sun, 21 Jun 2020 18:57:04: 5000000 INFO @ Sun, 21 Jun 2020 18:57:08: 16000000 INFO @ Sun, 21 Jun 2020 18:57:10: 11000000 INFO @ Sun, 21 Jun 2020 18:57:11: 6000000 INFO @ Sun, 21 Jun 2020 18:57:14: 17000000 INFO @ Sun, 21 Jun 2020 18:57:16: 12000000 INFO @ Sun, 21 Jun 2020 18:57:17: 7000000 INFO @ Sun, 21 Jun 2020 18:57:21: 18000000 INFO @ Sun, 21 Jun 2020 18:57:23: 13000000 INFO @ Sun, 21 Jun 2020 18:57:24: 8000000 INFO @ Sun, 21 Jun 2020 18:57:27: 19000000 INFO @ Sun, 21 Jun 2020 18:57:29: 14000000 INFO @ Sun, 21 Jun 2020 18:57:30: 9000000 INFO @ Sun, 21 Jun 2020 18:57:33: 20000000 INFO @ Sun, 21 Jun 2020 18:57:35: 15000000 INFO @ Sun, 21 Jun 2020 18:57:37: 10000000 INFO @ Sun, 21 Jun 2020 18:57:40: 21000000 INFO @ Sun, 21 Jun 2020 18:57:42: 16000000 INFO @ Sun, 21 Jun 2020 18:57:44: 11000000 INFO @ Sun, 21 Jun 2020 18:57:46: 22000000 INFO @ Sun, 21 Jun 2020 18:57:49: 17000000 INFO @ Sun, 21 Jun 2020 18:57:50: 12000000 INFO @ Sun, 21 Jun 2020 18:57:53: 23000000 INFO @ Sun, 21 Jun 2020 18:57:55: 18000000 INFO @ Sun, 21 Jun 2020 18:57:57: 13000000 INFO @ Sun, 21 Jun 2020 18:57:59: 24000000 INFO @ Sun, 21 Jun 2020 18:58:01: 19000000 INFO @ Sun, 21 Jun 2020 18:58:03: 14000000 INFO @ Sun, 21 Jun 2020 18:58:06: 25000000 INFO @ Sun, 21 Jun 2020 18:58:08: 20000000 INFO @ Sun, 21 Jun 2020 18:58:10: 15000000 INFO @ Sun, 21 Jun 2020 18:58:12: 26000000 INFO @ Sun, 21 Jun 2020 18:58:14: 21000000 INFO @ Sun, 21 Jun 2020 18:58:16: 16000000 INFO @ Sun, 21 Jun 2020 18:58:18: 27000000 INFO @ Sun, 21 Jun 2020 18:58:20: 22000000 INFO @ Sun, 21 Jun 2020 18:58:23: 17000000 INFO @ Sun, 21 Jun 2020 18:58:25: 28000000 INFO @ Sun, 21 Jun 2020 18:58:27: 23000000 INFO @ Sun, 21 Jun 2020 18:58:29: 18000000 INFO @ Sun, 21 Jun 2020 18:58:31: 29000000 INFO @ Sun, 21 Jun 2020 18:58:33: 24000000 INFO @ Sun, 21 Jun 2020 18:58:35: 19000000 INFO @ Sun, 21 Jun 2020 18:58:38: 30000000 INFO @ Sun, 21 Jun 2020 18:58:39: 25000000 INFO @ Sun, 21 Jun 2020 18:58:41: 20000000 INFO @ Sun, 21 Jun 2020 18:58:44: 31000000 INFO @ Sun, 21 Jun 2020 18:58:45: 26000000 INFO @ Sun, 21 Jun 2020 18:58:48: 21000000 INFO @ Sun, 21 Jun 2020 18:58:51: 32000000 INFO @ Sun, 21 Jun 2020 18:58:52: 27000000 INFO @ Sun, 21 Jun 2020 18:58:54: 22000000 INFO @ Sun, 21 Jun 2020 18:58:57: 33000000 INFO @ Sun, 21 Jun 2020 18:58:58: 28000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 18:59:00: 23000000 INFO @ Sun, 21 Jun 2020 18:59:04: 34000000 INFO @ Sun, 21 Jun 2020 18:59:04: 29000000 INFO @ Sun, 21 Jun 2020 18:59:07: 24000000 INFO @ Sun, 21 Jun 2020 18:59:09: #1 tag size is determined as 76 bps INFO @ Sun, 21 Jun 2020 18:59:09: #1 tag size = 76 INFO @ Sun, 21 Jun 2020 18:59:09: #1 total tags in treatment: 34800469 INFO @ Sun, 21 Jun 2020 18:59:09: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:59:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:59:10: #1 tags after filtering in treatment: 34800464 INFO @ Sun, 21 Jun 2020 18:59:10: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:59:10: #1 finished! INFO @ Sun, 21 Jun 2020 18:59:10: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:59:10: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:59:11: 30000000 INFO @ Sun, 21 Jun 2020 18:59:12: #2 number of paired peaks: 33 WARNING @ Sun, 21 Jun 2020 18:59:12: Too few paired peaks (33) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:59:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX2325655/SRX2325655.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2325655/SRX2325655.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2325655/SRX2325655.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2325655/SRX2325655.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 18:59:13: 25000000 INFO @ Sun, 21 Jun 2020 18:59:17: 31000000 INFO @ Sun, 21 Jun 2020 18:59:19: 26000000 INFO @ Sun, 21 Jun 2020 18:59:23: 32000000 INFO @ Sun, 21 Jun 2020 18:59:25: 27000000 INFO @ Sun, 21 Jun 2020 18:59:29: 33000000 INFO @ Sun, 21 Jun 2020 18:59:32: 28000000 INFO @ Sun, 21 Jun 2020 18:59:36: 34000000 INFO @ Sun, 21 Jun 2020 18:59:38: 29000000 INFO @ Sun, 21 Jun 2020 18:59:41: #1 tag size is determined as 76 bps INFO @ Sun, 21 Jun 2020 18:59:41: #1 tag size = 76 INFO @ Sun, 21 Jun 2020 18:59:41: #1 total tags in treatment: 34800469 INFO @ Sun, 21 Jun 2020 18:59:41: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:59:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:59:42: #1 tags after filtering in treatment: 34800464 INFO @ Sun, 21 Jun 2020 18:59:42: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:59:42: #1 finished! INFO @ Sun, 21 Jun 2020 18:59:42: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:59:42: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:59:44: #2 number of paired peaks: 33 WARNING @ Sun, 21 Jun 2020 18:59:44: Too few paired peaks (33) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:59:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX2325655/SRX2325655.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2325655/SRX2325655.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2325655/SRX2325655.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2325655/SRX2325655.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 18:59:44: 30000000 INFO @ Sun, 21 Jun 2020 18:59:50: 31000000 INFO @ Sun, 21 Jun 2020 18:59:56: 32000000 INFO @ Sun, 21 Jun 2020 19:00:02: 33000000 INFO @ Sun, 21 Jun 2020 19:00:08: 34000000 BigWig に変換しました。 INFO @ Sun, 21 Jun 2020 19:00:13: #1 tag size is determined as 76 bps INFO @ Sun, 21 Jun 2020 19:00:13: #1 tag size = 76 INFO @ Sun, 21 Jun 2020 19:00:13: #1 total tags in treatment: 34800469 INFO @ Sun, 21 Jun 2020 19:00:13: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:00:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:00:13: #1 tags after filtering in treatment: 34800464 INFO @ Sun, 21 Jun 2020 19:00:13: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:00:13: #1 finished! INFO @ Sun, 21 Jun 2020 19:00:13: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:00:13: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:00:16: #2 number of paired peaks: 33 WARNING @ Sun, 21 Jun 2020 19:00:16: Too few paired peaks (33) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:00:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX2325655/SRX2325655.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2325655/SRX2325655.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2325655/SRX2325655.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2325655/SRX2325655.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling