Job ID = 6454523 SRX = SRX220296 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T09:24:59 prefetch.2.10.7: 1) Downloading 'SRR653341'... 2020-06-21T09:24:59 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T09:31:56 prefetch.2.10.7: HTTPS download succeed 2020-06-21T09:31:56 prefetch.2.10.7: 1) 'SRR653341' was downloaded successfully Read 28554254 spots for SRR653341/SRR653341.sra Written 28554254 spots for SRR653341/SRR653341.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:04:38 28554254 reads; of these: 28554254 (100.00%) were unpaired; of these: 2706549 (9.48%) aligned 0 times 23346010 (81.76%) aligned exactly 1 time 2501695 (8.76%) aligned >1 times 90.52% overall alignment rate Time searching: 00:04:39 Overall time: 00:04:39 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3829353 / 25847705 = 0.1482 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:43:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX220296/SRX220296.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX220296/SRX220296.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX220296/SRX220296.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX220296/SRX220296.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:43:07: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:43:07: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:43:12: 1000000 INFO @ Sun, 21 Jun 2020 18:43:17: 2000000 INFO @ Sun, 21 Jun 2020 18:43:21: 3000000 INFO @ Sun, 21 Jun 2020 18:43:26: 4000000 INFO @ Sun, 21 Jun 2020 18:43:31: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:43:36: 6000000 INFO @ Sun, 21 Jun 2020 18:43:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX220296/SRX220296.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX220296/SRX220296.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX220296/SRX220296.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX220296/SRX220296.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:43:37: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:43:37: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:43:41: 7000000 INFO @ Sun, 21 Jun 2020 18:43:42: 1000000 INFO @ Sun, 21 Jun 2020 18:43:46: 8000000 INFO @ Sun, 21 Jun 2020 18:43:47: 2000000 INFO @ Sun, 21 Jun 2020 18:43:51: 9000000 INFO @ Sun, 21 Jun 2020 18:43:52: 3000000 INFO @ Sun, 21 Jun 2020 18:43:57: 10000000 INFO @ Sun, 21 Jun 2020 18:43:58: 4000000 INFO @ Sun, 21 Jun 2020 18:44:02: 11000000 INFO @ Sun, 21 Jun 2020 18:44:03: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:44:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX220296/SRX220296.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX220296/SRX220296.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX220296/SRX220296.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX220296/SRX220296.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:44:07: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:44:07: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:44:08: 12000000 INFO @ Sun, 21 Jun 2020 18:44:08: 6000000 INFO @ Sun, 21 Jun 2020 18:44:13: 1000000 INFO @ Sun, 21 Jun 2020 18:44:13: 13000000 INFO @ Sun, 21 Jun 2020 18:44:14: 7000000 INFO @ Sun, 21 Jun 2020 18:44:18: 2000000 INFO @ Sun, 21 Jun 2020 18:44:19: 14000000 INFO @ Sun, 21 Jun 2020 18:44:19: 8000000 INFO @ Sun, 21 Jun 2020 18:44:24: 3000000 INFO @ Sun, 21 Jun 2020 18:44:24: 15000000 INFO @ Sun, 21 Jun 2020 18:44:24: 9000000 INFO @ Sun, 21 Jun 2020 18:44:29: 4000000 INFO @ Sun, 21 Jun 2020 18:44:29: 16000000 INFO @ Sun, 21 Jun 2020 18:44:30: 10000000 INFO @ Sun, 21 Jun 2020 18:44:35: 17000000 INFO @ Sun, 21 Jun 2020 18:44:35: 5000000 INFO @ Sun, 21 Jun 2020 18:44:35: 11000000 INFO @ Sun, 21 Jun 2020 18:44:40: 6000000 INFO @ Sun, 21 Jun 2020 18:44:40: 18000000 INFO @ Sun, 21 Jun 2020 18:44:41: 12000000 INFO @ Sun, 21 Jun 2020 18:44:46: 19000000 INFO @ Sun, 21 Jun 2020 18:44:46: 7000000 INFO @ Sun, 21 Jun 2020 18:44:46: 13000000 INFO @ Sun, 21 Jun 2020 18:44:51: 20000000 INFO @ Sun, 21 Jun 2020 18:44:51: 8000000 INFO @ Sun, 21 Jun 2020 18:44:52: 14000000 INFO @ Sun, 21 Jun 2020 18:44:56: 21000000 INFO @ Sun, 21 Jun 2020 18:44:57: 9000000 INFO @ Sun, 21 Jun 2020 18:44:57: 15000000 INFO @ Sun, 21 Jun 2020 18:45:02: 22000000 INFO @ Sun, 21 Jun 2020 18:45:02: #1 tag size is determined as 36 bps INFO @ Sun, 21 Jun 2020 18:45:02: #1 tag size = 36 INFO @ Sun, 21 Jun 2020 18:45:02: #1 total tags in treatment: 22018352 INFO @ Sun, 21 Jun 2020 18:45:02: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:45:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:45:02: 16000000 INFO @ Sun, 21 Jun 2020 18:45:02: 10000000 INFO @ Sun, 21 Jun 2020 18:45:03: #1 tags after filtering in treatment: 22018269 INFO @ Sun, 21 Jun 2020 18:45:03: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:45:03: #1 finished! INFO @ Sun, 21 Jun 2020 18:45:03: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:45:03: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:45:04: #2 number of paired peaks: 74 WARNING @ Sun, 21 Jun 2020 18:45:04: Too few paired peaks (74) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:45:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX220296/SRX220296.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX220296/SRX220296.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX220296/SRX220296.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX220296/SRX220296.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 18:45:08: 17000000 INFO @ Sun, 21 Jun 2020 18:45:08: 11000000 INFO @ Sun, 21 Jun 2020 18:45:13: 18000000 INFO @ Sun, 21 Jun 2020 18:45:14: 12000000 INFO @ Sun, 21 Jun 2020 18:45:19: 19000000 INFO @ Sun, 21 Jun 2020 18:45:19: 13000000 INFO @ Sun, 21 Jun 2020 18:45:24: 20000000 INFO @ Sun, 21 Jun 2020 18:45:24: 14000000 INFO @ Sun, 21 Jun 2020 18:45:29: 21000000 INFO @ Sun, 21 Jun 2020 18:45:30: 15000000 INFO @ Sun, 21 Jun 2020 18:45:35: 22000000 INFO @ Sun, 21 Jun 2020 18:45:35: #1 tag size is determined as 36 bps INFO @ Sun, 21 Jun 2020 18:45:35: #1 tag size = 36 INFO @ Sun, 21 Jun 2020 18:45:35: #1 total tags in treatment: 22018352 INFO @ Sun, 21 Jun 2020 18:45:35: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:45:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:45:35: 16000000 INFO @ Sun, 21 Jun 2020 18:45:35: #1 tags after filtering in treatment: 22018269 INFO @ Sun, 21 Jun 2020 18:45:35: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:45:35: #1 finished! INFO @ Sun, 21 Jun 2020 18:45:35: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:45:35: #2 looking for paired plus/minus strand peaks... BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 18:45:37: #2 number of paired peaks: 74 WARNING @ Sun, 21 Jun 2020 18:45:37: Too few paired peaks (74) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:45:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX220296/SRX220296.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX220296/SRX220296.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX220296/SRX220296.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX220296/SRX220296.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 18:45:40: 17000000 INFO @ Sun, 21 Jun 2020 18:45:45: 18000000 INFO @ Sun, 21 Jun 2020 18:45:50: 19000000 INFO @ Sun, 21 Jun 2020 18:45:55: 20000000 INFO @ Sun, 21 Jun 2020 18:46:00: 21000000 INFO @ Sun, 21 Jun 2020 18:46:05: 22000000 INFO @ Sun, 21 Jun 2020 18:46:05: #1 tag size is determined as 36 bps INFO @ Sun, 21 Jun 2020 18:46:05: #1 tag size = 36 INFO @ Sun, 21 Jun 2020 18:46:05: #1 total tags in treatment: 22018352 INFO @ Sun, 21 Jun 2020 18:46:05: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:46:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:46:05: #1 tags after filtering in treatment: 22018269 INFO @ Sun, 21 Jun 2020 18:46:05: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:46:05: #1 finished! INFO @ Sun, 21 Jun 2020 18:46:05: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:46:05: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:46:07: #2 number of paired peaks: 74 WARNING @ Sun, 21 Jun 2020 18:46:07: Too few paired peaks (74) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:46:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX220296/SRX220296.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX220296/SRX220296.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX220296/SRX220296.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX220296/SRX220296.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。