Job ID = 6454506 SRX = SRX2172424 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T09:18:54 prefetch.2.10.7: 1) Downloading 'SRR4252435'... 2020-06-21T09:18:54 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T09:24:30 prefetch.2.10.7: HTTPS download succeed 2020-06-21T09:24:30 prefetch.2.10.7: 1) 'SRR4252435' was downloaded successfully 2020-06-21T09:24:30 prefetch.2.10.7: 'SRR4252435' has 0 unresolved dependencies Read 58597960 spots for SRR4252435/SRR4252435.sra Written 58597960 spots for SRR4252435/SRR4252435.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:15:38 58597960 reads; of these: 58597960 (100.00%) were unpaired; of these: 1755828 (3.00%) aligned 0 times 43302699 (73.90%) aligned exactly 1 time 13539433 (23.11%) aligned >1 times 97.00% overall alignment rate Time searching: 00:15:39 Overall time: 00:15:39 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 10330604 / 56842132 = 0.1817 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:59:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX2172424/SRX2172424.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX2172424/SRX2172424.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX2172424/SRX2172424.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX2172424/SRX2172424.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:59:08: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:59:08: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:59:13: 1000000 INFO @ Sun, 21 Jun 2020 18:59:19: 2000000 INFO @ Sun, 21 Jun 2020 18:59:24: 3000000 INFO @ Sun, 21 Jun 2020 18:59:30: 4000000 INFO @ Sun, 21 Jun 2020 18:59:35: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:59:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX2172424/SRX2172424.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX2172424/SRX2172424.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX2172424/SRX2172424.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX2172424/SRX2172424.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:59:38: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:59:38: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:59:41: 6000000 INFO @ Sun, 21 Jun 2020 18:59:44: 1000000 INFO @ Sun, 21 Jun 2020 18:59:47: 7000000 INFO @ Sun, 21 Jun 2020 18:59:50: 2000000 INFO @ Sun, 21 Jun 2020 18:59:53: 8000000 INFO @ Sun, 21 Jun 2020 18:59:56: 3000000 INFO @ Sun, 21 Jun 2020 19:00:00: 9000000 INFO @ Sun, 21 Jun 2020 19:00:02: 4000000 BedGraph に変換中... INFO @ Sun, 21 Jun 2020 19:00:06: 10000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 19:00:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX2172424/SRX2172424.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX2172424/SRX2172424.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX2172424/SRX2172424.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX2172424/SRX2172424.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 19:00:08: #1 read tag files... INFO @ Sun, 21 Jun 2020 19:00:08: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 19:00:09: 5000000 INFO @ Sun, 21 Jun 2020 19:00:12: 11000000 INFO @ Sun, 21 Jun 2020 19:00:14: 1000000 INFO @ Sun, 21 Jun 2020 19:00:15: 6000000 INFO @ Sun, 21 Jun 2020 19:00:18: 12000000 INFO @ Sun, 21 Jun 2020 19:00:20: 2000000 INFO @ Sun, 21 Jun 2020 19:00:21: 7000000 INFO @ Sun, 21 Jun 2020 19:00:24: 13000000 INFO @ Sun, 21 Jun 2020 19:00:26: 3000000 INFO @ Sun, 21 Jun 2020 19:00:28: 8000000 INFO @ Sun, 21 Jun 2020 19:00:30: 14000000 INFO @ Sun, 21 Jun 2020 19:00:32: 4000000 INFO @ Sun, 21 Jun 2020 19:00:34: 9000000 INFO @ Sun, 21 Jun 2020 19:00:36: 15000000 INFO @ Sun, 21 Jun 2020 19:00:38: 5000000 INFO @ Sun, 21 Jun 2020 19:00:41: 10000000 INFO @ Sun, 21 Jun 2020 19:00:43: 16000000 INFO @ Sun, 21 Jun 2020 19:00:45: 6000000 INFO @ Sun, 21 Jun 2020 19:00:47: 11000000 INFO @ Sun, 21 Jun 2020 19:00:49: 17000000 INFO @ Sun, 21 Jun 2020 19:00:51: 7000000 INFO @ Sun, 21 Jun 2020 19:00:53: 12000000 INFO @ Sun, 21 Jun 2020 19:00:55: 18000000 INFO @ Sun, 21 Jun 2020 19:00:57: 8000000 INFO @ Sun, 21 Jun 2020 19:01:00: 13000000 INFO @ Sun, 21 Jun 2020 19:01:01: 19000000 INFO @ Sun, 21 Jun 2020 19:01:03: 9000000 INFO @ Sun, 21 Jun 2020 19:01:06: 14000000 INFO @ Sun, 21 Jun 2020 19:01:07: 20000000 INFO @ Sun, 21 Jun 2020 19:01:09: 10000000 INFO @ Sun, 21 Jun 2020 19:01:12: 15000000 INFO @ Sun, 21 Jun 2020 19:01:13: 21000000 INFO @ Sun, 21 Jun 2020 19:01:16: 11000000 INFO @ Sun, 21 Jun 2020 19:01:18: 16000000 INFO @ Sun, 21 Jun 2020 19:01:19: 22000000 INFO @ Sun, 21 Jun 2020 19:01:22: 12000000 INFO @ Sun, 21 Jun 2020 19:01:24: 17000000 INFO @ Sun, 21 Jun 2020 19:01:26: 23000000 INFO @ Sun, 21 Jun 2020 19:01:28: 13000000 INFO @ Sun, 21 Jun 2020 19:01:31: 18000000 INFO @ Sun, 21 Jun 2020 19:01:32: 24000000 INFO @ Sun, 21 Jun 2020 19:01:34: 14000000 INFO @ Sun, 21 Jun 2020 19:01:37: 19000000 INFO @ Sun, 21 Jun 2020 19:01:38: 25000000 INFO @ Sun, 21 Jun 2020 19:01:40: 15000000 INFO @ Sun, 21 Jun 2020 19:01:43: 20000000 INFO @ Sun, 21 Jun 2020 19:01:44: 26000000 INFO @ Sun, 21 Jun 2020 19:01:47: 16000000 INFO @ Sun, 21 Jun 2020 19:01:49: 21000000 INFO @ Sun, 21 Jun 2020 19:01:51: 27000000 INFO @ Sun, 21 Jun 2020 19:01:53: 17000000 INFO @ Sun, 21 Jun 2020 19:01:56: 22000000 INFO @ Sun, 21 Jun 2020 19:01:57: 28000000 INFO @ Sun, 21 Jun 2020 19:01:59: 18000000 INFO @ Sun, 21 Jun 2020 19:02:02: 23000000 INFO @ Sun, 21 Jun 2020 19:02:03: 29000000 INFO @ Sun, 21 Jun 2020 19:02:06: 19000000 INFO @ Sun, 21 Jun 2020 19:02:08: 24000000 INFO @ Sun, 21 Jun 2020 19:02:10: 30000000 INFO @ Sun, 21 Jun 2020 19:02:12: 20000000 INFO @ Sun, 21 Jun 2020 19:02:15: 25000000 INFO @ Sun, 21 Jun 2020 19:02:16: 31000000 INFO @ Sun, 21 Jun 2020 19:02:18: 21000000 INFO @ Sun, 21 Jun 2020 19:02:21: 26000000 INFO @ Sun, 21 Jun 2020 19:02:22: 32000000 INFO @ Sun, 21 Jun 2020 19:02:24: 22000000 INFO @ Sun, 21 Jun 2020 19:02:27: 27000000 INFO @ Sun, 21 Jun 2020 19:02:28: 33000000 INFO @ Sun, 21 Jun 2020 19:02:31: 23000000 INFO @ Sun, 21 Jun 2020 19:02:33: 28000000 INFO @ Sun, 21 Jun 2020 19:02:34: 34000000 INFO @ Sun, 21 Jun 2020 19:02:37: 24000000 INFO @ Sun, 21 Jun 2020 19:02:40: 29000000 INFO @ Sun, 21 Jun 2020 19:02:40: 35000000 INFO @ Sun, 21 Jun 2020 19:02:43: 25000000 INFO @ Sun, 21 Jun 2020 19:02:46: 30000000 INFO @ Sun, 21 Jun 2020 19:02:47: 36000000 INFO @ Sun, 21 Jun 2020 19:02:50: 26000000 INFO @ Sun, 21 Jun 2020 19:02:52: 31000000 INFO @ Sun, 21 Jun 2020 19:02:53: 37000000 INFO @ Sun, 21 Jun 2020 19:02:56: 27000000 INFO @ Sun, 21 Jun 2020 19:02:58: 32000000 INFO @ Sun, 21 Jun 2020 19:02:59: 38000000 INFO @ Sun, 21 Jun 2020 19:03:02: 28000000 INFO @ Sun, 21 Jun 2020 19:03:05: 33000000 INFO @ Sun, 21 Jun 2020 19:03:05: 39000000 INFO @ Sun, 21 Jun 2020 19:03:08: 29000000 INFO @ Sun, 21 Jun 2020 19:03:11: 34000000 INFO @ Sun, 21 Jun 2020 19:03:12: 40000000 INFO @ Sun, 21 Jun 2020 19:03:15: 30000000 INFO @ Sun, 21 Jun 2020 19:03:17: 35000000 INFO @ Sun, 21 Jun 2020 19:03:18: 41000000 INFO @ Sun, 21 Jun 2020 19:03:21: 31000000 INFO @ Sun, 21 Jun 2020 19:03:23: 36000000 INFO @ Sun, 21 Jun 2020 19:03:24: 42000000 INFO @ Sun, 21 Jun 2020 19:03:28: 32000000 INFO @ Sun, 21 Jun 2020 19:03:30: 37000000 INFO @ Sun, 21 Jun 2020 19:03:31: 43000000 INFO @ Sun, 21 Jun 2020 19:03:34: 33000000 INFO @ Sun, 21 Jun 2020 19:03:36: 38000000 INFO @ Sun, 21 Jun 2020 19:03:37: 44000000 INFO @ Sun, 21 Jun 2020 19:03:41: 34000000 INFO @ Sun, 21 Jun 2020 19:03:43: 39000000 INFO @ Sun, 21 Jun 2020 19:03:43: 45000000 INFO @ Sun, 21 Jun 2020 19:03:47: 35000000 INFO @ Sun, 21 Jun 2020 19:03:50: 40000000 INFO @ Sun, 21 Jun 2020 19:03:50: 46000000 INFO @ Sun, 21 Jun 2020 19:03:54: 36000000 INFO @ Sun, 21 Jun 2020 19:03:54: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 19:03:54: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 19:03:54: #1 total tags in treatment: 46511528 INFO @ Sun, 21 Jun 2020 19:03:54: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:03:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:03:55: #1 tags after filtering in treatment: 46511483 INFO @ Sun, 21 Jun 2020 19:03:55: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:03:55: #1 finished! INFO @ Sun, 21 Jun 2020 19:03:55: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:03:55: #2 looking for paired plus/minus strand peaks... BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 19:03:56: 41000000 INFO @ Sun, 21 Jun 2020 19:03:58: #2 number of paired peaks: 33 WARNING @ Sun, 21 Jun 2020 19:03:58: Too few paired peaks (33) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:03:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX2172424/SRX2172424.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2172424/SRX2172424.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2172424/SRX2172424.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2172424/SRX2172424.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 19:04:01: 37000000 INFO @ Sun, 21 Jun 2020 19:04:02: 42000000 INFO @ Sun, 21 Jun 2020 19:04:07: 38000000 INFO @ Sun, 21 Jun 2020 19:04:09: 43000000 INFO @ Sun, 21 Jun 2020 19:04:14: 39000000 INFO @ Sun, 21 Jun 2020 19:04:15: 44000000 INFO @ Sun, 21 Jun 2020 19:04:21: 40000000 INFO @ Sun, 21 Jun 2020 19:04:21: 45000000 INFO @ Sun, 21 Jun 2020 19:04:27: 41000000 INFO @ Sun, 21 Jun 2020 19:04:28: 46000000 INFO @ Sun, 21 Jun 2020 19:04:31: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 19:04:31: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 19:04:31: #1 total tags in treatment: 46511528 INFO @ Sun, 21 Jun 2020 19:04:31: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:04:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:04:32: #1 tags after filtering in treatment: 46511483 INFO @ Sun, 21 Jun 2020 19:04:32: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:04:32: #1 finished! INFO @ Sun, 21 Jun 2020 19:04:32: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:04:32: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:04:33: 42000000 INFO @ Sun, 21 Jun 2020 19:04:35: #2 number of paired peaks: 33 WARNING @ Sun, 21 Jun 2020 19:04:35: Too few paired peaks (33) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:04:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX2172424/SRX2172424.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2172424/SRX2172424.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2172424/SRX2172424.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2172424/SRX2172424.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 19:04:39: 43000000 INFO @ Sun, 21 Jun 2020 19:04:45: 44000000 INFO @ Sun, 21 Jun 2020 19:04:51: 45000000 INFO @ Sun, 21 Jun 2020 19:04:56: 46000000 INFO @ Sun, 21 Jun 2020 19:05:00: #1 tag size is determined as 51 bps INFO @ Sun, 21 Jun 2020 19:05:00: #1 tag size = 51 INFO @ Sun, 21 Jun 2020 19:05:00: #1 total tags in treatment: 46511528 INFO @ Sun, 21 Jun 2020 19:05:00: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:05:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:05:01: #1 tags after filtering in treatment: 46511483 INFO @ Sun, 21 Jun 2020 19:05:01: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:05:01: #1 finished! INFO @ Sun, 21 Jun 2020 19:05:01: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:05:01: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:05:04: #2 number of paired peaks: 33 WARNING @ Sun, 21 Jun 2020 19:05:04: Too few paired peaks (33) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:05:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX2172424/SRX2172424.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2172424/SRX2172424.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2172424/SRX2172424.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2172424/SRX2172424.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。