Job ID = 6454338 SRX = SRX2011090 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T09:19:39 prefetch.2.10.7: 1) Downloading 'SRR4017243'... 2020-06-21T09:19:39 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T09:27:00 prefetch.2.10.7: HTTPS download succeed 2020-06-21T09:27:00 prefetch.2.10.7: 1) 'SRR4017243' was downloaded successfully Read 20908189 spots for SRR4017243/SRR4017243.sra Written 20908189 spots for SRR4017243/SRR4017243.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:47 20908189 reads; of these: 20908189 (100.00%) were unpaired; of these: 796424 (3.81%) aligned 0 times 17139616 (81.98%) aligned exactly 1 time 2972149 (14.22%) aligned >1 times 96.19% overall alignment rate Time searching: 00:08:47 Overall time: 00:08:47 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 1331108 / 20111765 = 0.0662 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:44:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX2011090/SRX2011090.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX2011090/SRX2011090.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX2011090/SRX2011090.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX2011090/SRX2011090.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:44:01: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:44:01: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:44:09: 1000000 INFO @ Sun, 21 Jun 2020 18:44:18: 2000000 INFO @ Sun, 21 Jun 2020 18:44:26: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:44:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX2011090/SRX2011090.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX2011090/SRX2011090.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX2011090/SRX2011090.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX2011090/SRX2011090.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:44:34: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:44:34: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:44:34: 4000000 INFO @ Sun, 21 Jun 2020 18:44:43: 1000000 INFO @ Sun, 21 Jun 2020 18:44:43: 5000000 INFO @ Sun, 21 Jun 2020 18:44:51: 2000000 INFO @ Sun, 21 Jun 2020 18:44:52: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:44:59: 3000000 INFO @ Sun, 21 Jun 2020 18:45:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX2011090/SRX2011090.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX2011090/SRX2011090.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX2011090/SRX2011090.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX2011090/SRX2011090.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:45:01: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:45:01: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:45:02: 7000000 INFO @ Sun, 21 Jun 2020 18:45:08: 4000000 INFO @ Sun, 21 Jun 2020 18:45:09: 1000000 INFO @ Sun, 21 Jun 2020 18:45:11: 8000000 INFO @ Sun, 21 Jun 2020 18:45:16: 5000000 INFO @ Sun, 21 Jun 2020 18:45:18: 2000000 INFO @ Sun, 21 Jun 2020 18:45:20: 9000000 INFO @ Sun, 21 Jun 2020 18:45:25: 6000000 INFO @ Sun, 21 Jun 2020 18:45:26: 3000000 INFO @ Sun, 21 Jun 2020 18:45:29: 10000000 INFO @ Sun, 21 Jun 2020 18:45:33: 7000000 INFO @ Sun, 21 Jun 2020 18:45:35: 4000000 INFO @ Sun, 21 Jun 2020 18:45:38: 11000000 INFO @ Sun, 21 Jun 2020 18:45:42: 8000000 INFO @ Sun, 21 Jun 2020 18:45:43: 5000000 INFO @ Sun, 21 Jun 2020 18:45:47: 12000000 INFO @ Sun, 21 Jun 2020 18:45:50: 9000000 INFO @ Sun, 21 Jun 2020 18:45:52: 6000000 INFO @ Sun, 21 Jun 2020 18:45:56: 13000000 INFO @ Sun, 21 Jun 2020 18:45:58: 10000000 INFO @ Sun, 21 Jun 2020 18:46:00: 7000000 INFO @ Sun, 21 Jun 2020 18:46:05: 14000000 INFO @ Sun, 21 Jun 2020 18:46:07: 11000000 INFO @ Sun, 21 Jun 2020 18:46:08: 8000000 INFO @ Sun, 21 Jun 2020 18:46:14: 15000000 INFO @ Sun, 21 Jun 2020 18:46:15: 12000000 INFO @ Sun, 21 Jun 2020 18:46:16: 9000000 INFO @ Sun, 21 Jun 2020 18:46:23: 16000000 INFO @ Sun, 21 Jun 2020 18:46:24: 13000000 INFO @ Sun, 21 Jun 2020 18:46:25: 10000000 INFO @ Sun, 21 Jun 2020 18:46:32: 14000000 INFO @ Sun, 21 Jun 2020 18:46:32: 17000000 INFO @ Sun, 21 Jun 2020 18:46:33: 11000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 18:46:40: 15000000 INFO @ Sun, 21 Jun 2020 18:46:41: 18000000 INFO @ Sun, 21 Jun 2020 18:46:41: 12000000 INFO @ Sun, 21 Jun 2020 18:46:48: #1 tag size is determined as 100 bps INFO @ Sun, 21 Jun 2020 18:46:48: #1 tag size = 100 INFO @ Sun, 21 Jun 2020 18:46:48: #1 total tags in treatment: 18780657 INFO @ Sun, 21 Jun 2020 18:46:48: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:46:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:46:49: #1 tags after filtering in treatment: 18780596 INFO @ Sun, 21 Jun 2020 18:46:49: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:46:49: #1 finished! INFO @ Sun, 21 Jun 2020 18:46:49: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:46:49: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:46:49: 16000000 INFO @ Sun, 21 Jun 2020 18:46:49: 13000000 INFO @ Sun, 21 Jun 2020 18:46:50: #2 number of paired peaks: 86 WARNING @ Sun, 21 Jun 2020 18:46:50: Too few paired peaks (86) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:46:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX2011090/SRX2011090.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2011090/SRX2011090.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2011090/SRX2011090.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2011090/SRX2011090.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 18:46:57: 14000000 INFO @ Sun, 21 Jun 2020 18:46:57: 17000000 INFO @ Sun, 21 Jun 2020 18:47:05: 15000000 INFO @ Sun, 21 Jun 2020 18:47:05: 18000000 INFO @ Sun, 21 Jun 2020 18:47:11: #1 tag size is determined as 100 bps INFO @ Sun, 21 Jun 2020 18:47:11: #1 tag size = 100 INFO @ Sun, 21 Jun 2020 18:47:11: #1 total tags in treatment: 18780657 INFO @ Sun, 21 Jun 2020 18:47:11: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:47:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:47:12: #1 tags after filtering in treatment: 18780596 INFO @ Sun, 21 Jun 2020 18:47:12: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:47:12: #1 finished! INFO @ Sun, 21 Jun 2020 18:47:12: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:47:12: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:47:13: 16000000 INFO @ Sun, 21 Jun 2020 18:47:13: #2 number of paired peaks: 86 WARNING @ Sun, 21 Jun 2020 18:47:13: Too few paired peaks (86) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:47:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX2011090/SRX2011090.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2011090/SRX2011090.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2011090/SRX2011090.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2011090/SRX2011090.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。 INFO @ Sun, 21 Jun 2020 18:47:20: 17000000 INFO @ Sun, 21 Jun 2020 18:47:27: 18000000 INFO @ Sun, 21 Jun 2020 18:47:33: #1 tag size is determined as 100 bps INFO @ Sun, 21 Jun 2020 18:47:33: #1 tag size = 100 INFO @ Sun, 21 Jun 2020 18:47:33: #1 total tags in treatment: 18780657 INFO @ Sun, 21 Jun 2020 18:47:33: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:47:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:47:33: #1 tags after filtering in treatment: 18780596 INFO @ Sun, 21 Jun 2020 18:47:33: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:47:33: #1 finished! INFO @ Sun, 21 Jun 2020 18:47:33: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:47:33: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:47:35: #2 number of paired peaks: 86 WARNING @ Sun, 21 Jun 2020 18:47:35: Too few paired peaks (86) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:47:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX2011090/SRX2011090.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2011090/SRX2011090.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2011090/SRX2011090.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX2011090/SRX2011090.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling