Job ID = 6454207 SRX = SRX1872294 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T09:18:54 prefetch.2.10.7: 1) Downloading 'SRR3712755'... 2020-06-21T09:18:54 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T09:28:53 prefetch.2.10.7: HTTPS download succeed 2020-06-21T09:28:53 prefetch.2.10.7: 1) 'SRR3712755' was downloaded successfully Read 51140598 spots for SRR3712755/SRR3712755.sra Written 51140598 spots for SRR3712755/SRR3712755.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:36 51140598 reads; of these: 51140598 (100.00%) were unpaired; of these: 3972917 (7.77%) aligned 0 times 43130732 (84.34%) aligned exactly 1 time 4036949 (7.89%) aligned >1 times 92.23% overall alignment rate Time searching: 00:10:36 Overall time: 00:10:36 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 12357969 / 47167681 = 0.2620 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:55:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX1872294/SRX1872294.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX1872294/SRX1872294.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX1872294/SRX1872294.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX1872294/SRX1872294.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:55:48: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:55:48: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:55:53: 1000000 INFO @ Sun, 21 Jun 2020 18:55:58: 2000000 INFO @ Sun, 21 Jun 2020 18:56:03: 3000000 INFO @ Sun, 21 Jun 2020 18:56:08: 4000000 INFO @ Sun, 21 Jun 2020 18:56:13: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:56:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX1872294/SRX1872294.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX1872294/SRX1872294.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX1872294/SRX1872294.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX1872294/SRX1872294.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:56:18: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:56:18: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:56:18: 6000000 INFO @ Sun, 21 Jun 2020 18:56:23: 1000000 INFO @ Sun, 21 Jun 2020 18:56:23: 7000000 INFO @ Sun, 21 Jun 2020 18:56:29: 2000000 INFO @ Sun, 21 Jun 2020 18:56:29: 8000000 INFO @ Sun, 21 Jun 2020 18:56:34: 3000000 INFO @ Sun, 21 Jun 2020 18:56:34: 9000000 INFO @ Sun, 21 Jun 2020 18:56:39: 4000000 INFO @ Sun, 21 Jun 2020 18:56:39: 10000000 INFO @ Sun, 21 Jun 2020 18:56:45: 5000000 INFO @ Sun, 21 Jun 2020 18:56:45: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:56:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX1872294/SRX1872294.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX1872294/SRX1872294.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX1872294/SRX1872294.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX1872294/SRX1872294.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:56:48: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:56:48: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:56:50: 6000000 INFO @ Sun, 21 Jun 2020 18:56:50: 12000000 INFO @ Sun, 21 Jun 2020 18:56:54: 1000000 INFO @ Sun, 21 Jun 2020 18:56:56: 7000000 INFO @ Sun, 21 Jun 2020 18:56:56: 13000000 INFO @ Sun, 21 Jun 2020 18:57:00: 2000000 INFO @ Sun, 21 Jun 2020 18:57:02: 8000000 INFO @ Sun, 21 Jun 2020 18:57:02: 14000000 INFO @ Sun, 21 Jun 2020 18:57:06: 3000000 INFO @ Sun, 21 Jun 2020 18:57:08: 9000000 INFO @ Sun, 21 Jun 2020 18:57:08: 15000000 INFO @ Sun, 21 Jun 2020 18:57:12: 4000000 INFO @ Sun, 21 Jun 2020 18:57:14: 10000000 INFO @ Sun, 21 Jun 2020 18:57:14: 16000000 INFO @ Sun, 21 Jun 2020 18:57:18: 5000000 INFO @ Sun, 21 Jun 2020 18:57:20: 17000000 INFO @ Sun, 21 Jun 2020 18:57:20: 11000000 INFO @ Sun, 21 Jun 2020 18:57:24: 6000000 INFO @ Sun, 21 Jun 2020 18:57:25: 18000000 INFO @ Sun, 21 Jun 2020 18:57:26: 12000000 INFO @ Sun, 21 Jun 2020 18:57:30: 7000000 INFO @ Sun, 21 Jun 2020 18:57:31: 19000000 INFO @ Sun, 21 Jun 2020 18:57:32: 13000000 INFO @ Sun, 21 Jun 2020 18:57:36: 8000000 INFO @ Sun, 21 Jun 2020 18:57:37: 20000000 INFO @ Sun, 21 Jun 2020 18:57:38: 14000000 INFO @ Sun, 21 Jun 2020 18:57:42: 9000000 INFO @ Sun, 21 Jun 2020 18:57:43: 21000000 INFO @ Sun, 21 Jun 2020 18:57:44: 15000000 INFO @ Sun, 21 Jun 2020 18:57:48: 10000000 INFO @ Sun, 21 Jun 2020 18:57:49: 22000000 INFO @ Sun, 21 Jun 2020 18:57:49: 16000000 INFO @ Sun, 21 Jun 2020 18:57:54: 11000000 INFO @ Sun, 21 Jun 2020 18:57:55: 23000000 INFO @ Sun, 21 Jun 2020 18:57:55: 17000000 INFO @ Sun, 21 Jun 2020 18:58:00: 12000000 INFO @ Sun, 21 Jun 2020 18:58:01: 24000000 INFO @ Sun, 21 Jun 2020 18:58:01: 18000000 INFO @ Sun, 21 Jun 2020 18:58:06: 13000000 INFO @ Sun, 21 Jun 2020 18:58:07: 25000000 INFO @ Sun, 21 Jun 2020 18:58:07: 19000000 INFO @ Sun, 21 Jun 2020 18:58:13: 14000000 INFO @ Sun, 21 Jun 2020 18:58:13: 26000000 INFO @ Sun, 21 Jun 2020 18:58:13: 20000000 INFO @ Sun, 21 Jun 2020 18:58:19: 15000000 INFO @ Sun, 21 Jun 2020 18:58:19: 27000000 INFO @ Sun, 21 Jun 2020 18:58:20: 21000000 INFO @ Sun, 21 Jun 2020 18:58:25: 16000000 INFO @ Sun, 21 Jun 2020 18:58:25: 28000000 INFO @ Sun, 21 Jun 2020 18:58:26: 22000000 INFO @ Sun, 21 Jun 2020 18:58:31: 17000000 INFO @ Sun, 21 Jun 2020 18:58:32: 23000000 INFO @ Sun, 21 Jun 2020 18:58:32: 29000000 INFO @ Sun, 21 Jun 2020 18:58:37: 18000000 INFO @ Sun, 21 Jun 2020 18:58:38: 24000000 INFO @ Sun, 21 Jun 2020 18:58:39: 30000000 INFO @ Sun, 21 Jun 2020 18:58:43: 19000000 INFO @ Sun, 21 Jun 2020 18:58:44: 25000000 INFO @ Sun, 21 Jun 2020 18:58:45: 31000000 INFO @ Sun, 21 Jun 2020 18:58:50: 20000000 INFO @ Sun, 21 Jun 2020 18:58:50: 26000000 INFO @ Sun, 21 Jun 2020 18:58:51: 32000000 INFO @ Sun, 21 Jun 2020 18:58:56: 21000000 INFO @ Sun, 21 Jun 2020 18:58:56: 27000000 INFO @ Sun, 21 Jun 2020 18:58:57: 33000000 INFO @ Sun, 21 Jun 2020 18:59:02: 22000000 INFO @ Sun, 21 Jun 2020 18:59:03: 28000000 INFO @ Sun, 21 Jun 2020 18:59:04: 34000000 INFO @ Sun, 21 Jun 2020 18:59:08: 23000000 INFO @ Sun, 21 Jun 2020 18:59:10: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 18:59:10: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 18:59:10: #1 total tags in treatment: 34809712 INFO @ Sun, 21 Jun 2020 18:59:10: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:59:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:59:10: 29000000 INFO @ Sun, 21 Jun 2020 18:59:11: #1 tags after filtering in treatment: 34809674 INFO @ Sun, 21 Jun 2020 18:59:11: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:59:11: #1 finished! INFO @ Sun, 21 Jun 2020 18:59:11: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:59:11: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:59:13: #2 number of paired peaks: 14 WARNING @ Sun, 21 Jun 2020 18:59:13: Too few paired peaks (14) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:59:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX1872294/SRX1872294.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1872294/SRX1872294.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1872294/SRX1872294.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1872294/SRX1872294.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 18:59:14: 24000000 INFO @ Sun, 21 Jun 2020 18:59:16: 30000000 INFO @ Sun, 21 Jun 2020 18:59:20: 25000000 INFO @ Sun, 21 Jun 2020 18:59:21: 31000000 INFO @ Sun, 21 Jun 2020 18:59:26: 26000000 INFO @ Sun, 21 Jun 2020 18:59:27: 32000000 INFO @ Sun, 21 Jun 2020 18:59:32: 27000000 INFO @ Sun, 21 Jun 2020 18:59:33: 33000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 18:59:38: 28000000 INFO @ Sun, 21 Jun 2020 18:59:39: 34000000 INFO @ Sun, 21 Jun 2020 18:59:44: 29000000 INFO @ Sun, 21 Jun 2020 18:59:44: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 18:59:44: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 18:59:44: #1 total tags in treatment: 34809712 INFO @ Sun, 21 Jun 2020 18:59:44: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:59:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:59:46: #1 tags after filtering in treatment: 34809674 INFO @ Sun, 21 Jun 2020 18:59:46: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:59:46: #1 finished! INFO @ Sun, 21 Jun 2020 18:59:46: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:59:46: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:59:48: #2 number of paired peaks: 14 WARNING @ Sun, 21 Jun 2020 18:59:48: Too few paired peaks (14) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:59:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX1872294/SRX1872294.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1872294/SRX1872294.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1872294/SRX1872294.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1872294/SRX1872294.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 18:59:50: 30000000 INFO @ Sun, 21 Jun 2020 18:59:55: 31000000 INFO @ Sun, 21 Jun 2020 19:00:00: 32000000 INFO @ Sun, 21 Jun 2020 19:00:05: 33000000 INFO @ Sun, 21 Jun 2020 19:00:11: 34000000 INFO @ Sun, 21 Jun 2020 19:00:16: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 19:00:16: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 19:00:16: #1 total tags in treatment: 34809712 INFO @ Sun, 21 Jun 2020 19:00:16: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 19:00:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 19:00:17: #1 tags after filtering in treatment: 34809674 INFO @ Sun, 21 Jun 2020 19:00:17: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 19:00:17: #1 finished! INFO @ Sun, 21 Jun 2020 19:00:17: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 19:00:17: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 19:00:19: #2 number of paired peaks: 14 WARNING @ Sun, 21 Jun 2020 19:00:19: Too few paired peaks (14) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 19:00:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX1872294/SRX1872294.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1872294/SRX1872294.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1872294/SRX1872294.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1872294/SRX1872294.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。