Job ID = 6454203 SRX = SRX1872290 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T09:00:19 prefetch.2.10.7: 1) Downloading 'SRR3712751'... 2020-06-21T09:00:19 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T09:09:25 prefetch.2.10.7: HTTPS download succeed 2020-06-21T09:09:25 prefetch.2.10.7: 1) 'SRR3712751' was downloaded successfully Read 46046872 spots for SRR3712751/SRR3712751.sra Written 46046872 spots for SRR3712751/SRR3712751.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:04 46046872 reads; of these: 46046872 (100.00%) were unpaired; of these: 3465183 (7.53%) aligned 0 times 37144050 (80.67%) aligned exactly 1 time 5437639 (11.81%) aligned >1 times 92.47% overall alignment rate Time searching: 00:10:04 Overall time: 00:10:04 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 7942213 / 42581689 = 0.1865 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:31:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX1872290/SRX1872290.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX1872290/SRX1872290.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX1872290/SRX1872290.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX1872290/SRX1872290.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:31:29: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:31:29: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:31:36: 1000000 INFO @ Sun, 21 Jun 2020 18:31:42: 2000000 INFO @ Sun, 21 Jun 2020 18:31:48: 3000000 INFO @ Sun, 21 Jun 2020 18:31:54: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:31:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX1872290/SRX1872290.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX1872290/SRX1872290.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX1872290/SRX1872290.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX1872290/SRX1872290.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:31:59: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:31:59: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:32:01: 5000000 INFO @ Sun, 21 Jun 2020 18:32:06: 1000000 INFO @ Sun, 21 Jun 2020 18:32:07: 6000000 INFO @ Sun, 21 Jun 2020 18:32:13: 2000000 INFO @ Sun, 21 Jun 2020 18:32:14: 7000000 INFO @ Sun, 21 Jun 2020 18:32:19: 3000000 INFO @ Sun, 21 Jun 2020 18:32:21: 8000000 INFO @ Sun, 21 Jun 2020 18:32:26: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:32:28: 9000000 INFO @ Sun, 21 Jun 2020 18:32:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX1872290/SRX1872290.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX1872290/SRX1872290.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX1872290/SRX1872290.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX1872290/SRX1872290.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:32:30: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:32:30: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:32:33: 5000000 INFO @ Sun, 21 Jun 2020 18:32:35: 10000000 INFO @ Sun, 21 Jun 2020 18:32:36: 1000000 INFO @ Sun, 21 Jun 2020 18:32:39: 6000000 INFO @ Sun, 21 Jun 2020 18:32:42: 11000000 INFO @ Sun, 21 Jun 2020 18:32:43: 2000000 INFO @ Sun, 21 Jun 2020 18:32:46: 7000000 INFO @ Sun, 21 Jun 2020 18:32:50: 12000000 INFO @ Sun, 21 Jun 2020 18:32:50: 3000000 INFO @ Sun, 21 Jun 2020 18:32:53: 8000000 INFO @ Sun, 21 Jun 2020 18:32:57: 13000000 INFO @ Sun, 21 Jun 2020 18:32:57: 4000000 INFO @ Sun, 21 Jun 2020 18:33:00: 9000000 INFO @ Sun, 21 Jun 2020 18:33:04: 14000000 INFO @ Sun, 21 Jun 2020 18:33:04: 5000000 INFO @ Sun, 21 Jun 2020 18:33:07: 10000000 INFO @ Sun, 21 Jun 2020 18:33:10: 15000000 INFO @ Sun, 21 Jun 2020 18:33:11: 6000000 INFO @ Sun, 21 Jun 2020 18:33:14: 11000000 INFO @ Sun, 21 Jun 2020 18:33:17: 16000000 INFO @ Sun, 21 Jun 2020 18:33:18: 7000000 INFO @ Sun, 21 Jun 2020 18:33:21: 12000000 INFO @ Sun, 21 Jun 2020 18:33:24: 17000000 INFO @ Sun, 21 Jun 2020 18:33:24: 8000000 INFO @ Sun, 21 Jun 2020 18:33:28: 13000000 INFO @ Sun, 21 Jun 2020 18:33:30: 18000000 INFO @ Sun, 21 Jun 2020 18:33:31: 9000000 INFO @ Sun, 21 Jun 2020 18:33:35: 14000000 INFO @ Sun, 21 Jun 2020 18:33:37: 19000000 INFO @ Sun, 21 Jun 2020 18:33:38: 10000000 INFO @ Sun, 21 Jun 2020 18:33:42: 15000000 INFO @ Sun, 21 Jun 2020 18:33:44: 20000000 INFO @ Sun, 21 Jun 2020 18:33:45: 11000000 INFO @ Sun, 21 Jun 2020 18:33:49: 16000000 INFO @ Sun, 21 Jun 2020 18:33:51: 21000000 INFO @ Sun, 21 Jun 2020 18:33:52: 12000000 INFO @ Sun, 21 Jun 2020 18:33:56: 17000000 INFO @ Sun, 21 Jun 2020 18:33:58: 22000000 INFO @ Sun, 21 Jun 2020 18:33:59: 13000000 INFO @ Sun, 21 Jun 2020 18:34:03: 18000000 INFO @ Sun, 21 Jun 2020 18:34:04: 23000000 INFO @ Sun, 21 Jun 2020 18:34:06: 14000000 INFO @ Sun, 21 Jun 2020 18:34:09: 19000000 INFO @ Sun, 21 Jun 2020 18:34:11: 24000000 INFO @ Sun, 21 Jun 2020 18:34:13: 15000000 INFO @ Sun, 21 Jun 2020 18:34:16: 20000000 INFO @ Sun, 21 Jun 2020 18:34:18: 25000000 INFO @ Sun, 21 Jun 2020 18:34:20: 16000000 INFO @ Sun, 21 Jun 2020 18:34:23: 21000000 INFO @ Sun, 21 Jun 2020 18:34:24: 26000000 INFO @ Sun, 21 Jun 2020 18:34:27: 17000000 INFO @ Sun, 21 Jun 2020 18:34:29: 22000000 INFO @ Sun, 21 Jun 2020 18:34:31: 27000000 INFO @ Sun, 21 Jun 2020 18:34:34: 18000000 INFO @ Sun, 21 Jun 2020 18:34:36: 23000000 INFO @ Sun, 21 Jun 2020 18:34:38: 28000000 INFO @ Sun, 21 Jun 2020 18:34:41: 19000000 INFO @ Sun, 21 Jun 2020 18:34:43: 24000000 INFO @ Sun, 21 Jun 2020 18:34:45: 29000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 18:34:48: 20000000 INFO @ Sun, 21 Jun 2020 18:34:49: 25000000 INFO @ Sun, 21 Jun 2020 18:34:52: 30000000 INFO @ Sun, 21 Jun 2020 18:34:55: 21000000 INFO @ Sun, 21 Jun 2020 18:34:56: 26000000 INFO @ Sun, 21 Jun 2020 18:34:59: 31000000 INFO @ Sun, 21 Jun 2020 18:35:02: 22000000 INFO @ Sun, 21 Jun 2020 18:35:02: 27000000 INFO @ Sun, 21 Jun 2020 18:35:05: 32000000 INFO @ Sun, 21 Jun 2020 18:35:08: 23000000 INFO @ Sun, 21 Jun 2020 18:35:09: 28000000 INFO @ Sun, 21 Jun 2020 18:35:12: 33000000 INFO @ Sun, 21 Jun 2020 18:35:15: 24000000 INFO @ Sun, 21 Jun 2020 18:35:17: 29000000 INFO @ Sun, 21 Jun 2020 18:35:19: 34000000 INFO @ Sun, 21 Jun 2020 18:35:22: 25000000 INFO @ Sun, 21 Jun 2020 18:35:23: 30000000 INFO @ Sun, 21 Jun 2020 18:35:24: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 18:35:24: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 18:35:24: #1 total tags in treatment: 34639476 INFO @ Sun, 21 Jun 2020 18:35:24: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:35:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:35:25: #1 tags after filtering in treatment: 34639476 INFO @ Sun, 21 Jun 2020 18:35:25: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:35:25: #1 finished! INFO @ Sun, 21 Jun 2020 18:35:25: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:35:25: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:35:27: #2 number of paired peaks: 94 WARNING @ Sun, 21 Jun 2020 18:35:27: Too few paired peaks (94) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:35:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX1872290/SRX1872290.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1872290/SRX1872290.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1872290/SRX1872290.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1872290/SRX1872290.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 18:35:29: 26000000 INFO @ Sun, 21 Jun 2020 18:35:30: 31000000 INFO @ Sun, 21 Jun 2020 18:35:35: 27000000 INFO @ Sun, 21 Jun 2020 18:35:37: 32000000 INFO @ Sun, 21 Jun 2020 18:35:42: 28000000 INFO @ Sun, 21 Jun 2020 18:35:44: 33000000 INFO @ Sun, 21 Jun 2020 18:35:49: 29000000 INFO @ Sun, 21 Jun 2020 18:35:50: 34000000 BigWig に変換しました。 INFO @ Sun, 21 Jun 2020 18:35:55: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 18:35:55: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 18:35:55: #1 total tags in treatment: 34639476 INFO @ Sun, 21 Jun 2020 18:35:55: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:35:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:35:56: 30000000 INFO @ Sun, 21 Jun 2020 18:35:56: #1 tags after filtering in treatment: 34639476 INFO @ Sun, 21 Jun 2020 18:35:56: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:35:56: #1 finished! INFO @ Sun, 21 Jun 2020 18:35:56: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:35:56: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:35:58: #2 number of paired peaks: 94 WARNING @ Sun, 21 Jun 2020 18:35:58: Too few paired peaks (94) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:35:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX1872290/SRX1872290.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1872290/SRX1872290.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1872290/SRX1872290.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1872290/SRX1872290.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 18:36:02: 31000000 INFO @ Sun, 21 Jun 2020 18:36:08: 32000000 INFO @ Sun, 21 Jun 2020 18:36:14: 33000000 INFO @ Sun, 21 Jun 2020 18:36:20: 34000000 INFO @ Sun, 21 Jun 2020 18:36:25: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 18:36:25: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 18:36:25: #1 total tags in treatment: 34639476 INFO @ Sun, 21 Jun 2020 18:36:25: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:36:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:36:26: #1 tags after filtering in treatment: 34639476 INFO @ Sun, 21 Jun 2020 18:36:26: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:36:26: #1 finished! INFO @ Sun, 21 Jun 2020 18:36:26: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:36:26: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:36:28: #2 number of paired peaks: 94 WARNING @ Sun, 21 Jun 2020 18:36:28: Too few paired peaks (94) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:36:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX1872290/SRX1872290.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1872290/SRX1872290.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1872290/SRX1872290.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1872290/SRX1872290.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling