Job ID = 6454172 SRX = SRX1847049 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T09:07:09 prefetch.2.10.7: 1) Downloading 'SRR3669866'... 2020-06-21T09:07:09 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T09:14:01 prefetch.2.10.7: HTTPS download succeed 2020-06-21T09:14:01 prefetch.2.10.7: 1) 'SRR3669866' was downloaded successfully Read 41989083 spots for SRR3669866/SRR3669866.sra Written 41989083 spots for SRR3669866/SRR3669866.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:01 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:31 41989083 reads; of these: 41989083 (100.00%) were unpaired; of these: 1120042 (2.67%) aligned 0 times 35843491 (85.36%) aligned exactly 1 time 5025550 (11.97%) aligned >1 times 97.33% overall alignment rate Time searching: 00:09:32 Overall time: 00:09:32 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 13124466 / 40869041 = 0.3211 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:34:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX1847049/SRX1847049.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX1847049/SRX1847049.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX1847049/SRX1847049.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX1847049/SRX1847049.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:34:56: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:34:56: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:35:01: 1000000 INFO @ Sun, 21 Jun 2020 18:35:07: 2000000 INFO @ Sun, 21 Jun 2020 18:35:12: 3000000 INFO @ Sun, 21 Jun 2020 18:35:18: 4000000 INFO @ Sun, 21 Jun 2020 18:35:23: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:35:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX1847049/SRX1847049.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX1847049/SRX1847049.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX1847049/SRX1847049.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX1847049/SRX1847049.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:35:26: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:35:26: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:35:29: 6000000 INFO @ Sun, 21 Jun 2020 18:35:32: 1000000 INFO @ Sun, 21 Jun 2020 18:35:34: 7000000 INFO @ Sun, 21 Jun 2020 18:35:37: 2000000 INFO @ Sun, 21 Jun 2020 18:35:40: 8000000 INFO @ Sun, 21 Jun 2020 18:35:43: 3000000 INFO @ Sun, 21 Jun 2020 18:35:46: 9000000 INFO @ Sun, 21 Jun 2020 18:35:49: 4000000 INFO @ Sun, 21 Jun 2020 18:35:52: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:35:55: 5000000 INFO @ Sun, 21 Jun 2020 18:35:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX1847049/SRX1847049.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX1847049/SRX1847049.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX1847049/SRX1847049.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX1847049/SRX1847049.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:35:56: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:35:56: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:35:58: 11000000 INFO @ Sun, 21 Jun 2020 18:36:01: 6000000 INFO @ Sun, 21 Jun 2020 18:36:02: 1000000 INFO @ Sun, 21 Jun 2020 18:36:04: 12000000 INFO @ Sun, 21 Jun 2020 18:36:07: 7000000 INFO @ Sun, 21 Jun 2020 18:36:08: 2000000 INFO @ Sun, 21 Jun 2020 18:36:10: 13000000 INFO @ Sun, 21 Jun 2020 18:36:13: 8000000 INFO @ Sun, 21 Jun 2020 18:36:14: 3000000 INFO @ Sun, 21 Jun 2020 18:36:16: 14000000 INFO @ Sun, 21 Jun 2020 18:36:19: 9000000 INFO @ Sun, 21 Jun 2020 18:36:20: 4000000 INFO @ Sun, 21 Jun 2020 18:36:23: 15000000 INFO @ Sun, 21 Jun 2020 18:36:26: 10000000 INFO @ Sun, 21 Jun 2020 18:36:26: 5000000 INFO @ Sun, 21 Jun 2020 18:36:29: 16000000 INFO @ Sun, 21 Jun 2020 18:36:32: 11000000 INFO @ Sun, 21 Jun 2020 18:36:33: 6000000 INFO @ Sun, 21 Jun 2020 18:36:35: 17000000 INFO @ Sun, 21 Jun 2020 18:36:38: 12000000 INFO @ Sun, 21 Jun 2020 18:36:39: 7000000 INFO @ Sun, 21 Jun 2020 18:36:41: 18000000 INFO @ Sun, 21 Jun 2020 18:36:44: 13000000 INFO @ Sun, 21 Jun 2020 18:36:44: 8000000 INFO @ Sun, 21 Jun 2020 18:36:47: 19000000 INFO @ Sun, 21 Jun 2020 18:36:50: 14000000 INFO @ Sun, 21 Jun 2020 18:36:50: 9000000 INFO @ Sun, 21 Jun 2020 18:36:53: 20000000 INFO @ Sun, 21 Jun 2020 18:36:56: 15000000 INFO @ Sun, 21 Jun 2020 18:36:57: 10000000 INFO @ Sun, 21 Jun 2020 18:36:59: 21000000 INFO @ Sun, 21 Jun 2020 18:37:02: 16000000 INFO @ Sun, 21 Jun 2020 18:37:03: 11000000 INFO @ Sun, 21 Jun 2020 18:37:05: 22000000 INFO @ Sun, 21 Jun 2020 18:37:08: 17000000 INFO @ Sun, 21 Jun 2020 18:37:09: 12000000 INFO @ Sun, 21 Jun 2020 18:37:12: 23000000 INFO @ Sun, 21 Jun 2020 18:37:14: 18000000 INFO @ Sun, 21 Jun 2020 18:37:16: 13000000 INFO @ Sun, 21 Jun 2020 18:37:19: 24000000 INFO @ Sun, 21 Jun 2020 18:37:21: 19000000 INFO @ Sun, 21 Jun 2020 18:37:24: 14000000 INFO @ Sun, 21 Jun 2020 18:37:27: 25000000 INFO @ Sun, 21 Jun 2020 18:37:29: 20000000 INFO @ Sun, 21 Jun 2020 18:37:30: 15000000 INFO @ Sun, 21 Jun 2020 18:37:34: 26000000 INFO @ Sun, 21 Jun 2020 18:37:36: 21000000 INFO @ Sun, 21 Jun 2020 18:37:38: 16000000 INFO @ Sun, 21 Jun 2020 18:37:40: 27000000 INFO @ Sun, 21 Jun 2020 18:37:43: 22000000 INFO @ Sun, 21 Jun 2020 18:37:45: 17000000 INFO @ Sun, 21 Jun 2020 18:37:46: #1 tag size is determined as 49 bps INFO @ Sun, 21 Jun 2020 18:37:46: #1 tag size = 49 INFO @ Sun, 21 Jun 2020 18:37:46: #1 total tags in treatment: 27744575 INFO @ Sun, 21 Jun 2020 18:37:46: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:37:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:37:47: #1 tags after filtering in treatment: 27744575 INFO @ Sun, 21 Jun 2020 18:37:47: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:37:47: #1 finished! INFO @ Sun, 21 Jun 2020 18:37:47: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:37:47: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:37:49: #2 number of paired peaks: 4 WARNING @ Sun, 21 Jun 2020 18:37:49: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:37:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX1847049/SRX1847049.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 4 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1847049/SRX1847049.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1847049/SRX1847049.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1847049/SRX1847049.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 18:37:50: 23000000 INFO @ Sun, 21 Jun 2020 18:37:52: 18000000 INFO @ Sun, 21 Jun 2020 18:37:56: 24000000 INFO @ Sun, 21 Jun 2020 18:37:57: 19000000 INFO @ Sun, 21 Jun 2020 18:38:02: 25000000 INFO @ Sun, 21 Jun 2020 18:38:03: 20000000 INFO @ Sun, 21 Jun 2020 18:38:08: 26000000 INFO @ Sun, 21 Jun 2020 18:38:09: 21000000 INFO @ Sun, 21 Jun 2020 18:38:14: 27000000 INFO @ Sun, 21 Jun 2020 18:38:15: 22000000 INFO @ Sun, 21 Jun 2020 18:38:19: #1 tag size is determined as 49 bps INFO @ Sun, 21 Jun 2020 18:38:19: #1 tag size = 49 INFO @ Sun, 21 Jun 2020 18:38:19: #1 total tags in treatment: 27744575 INFO @ Sun, 21 Jun 2020 18:38:19: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:38:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:38:20: #1 tags after filtering in treatment: 27744575 INFO @ Sun, 21 Jun 2020 18:38:20: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:38:20: #1 finished! INFO @ Sun, 21 Jun 2020 18:38:20: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:38:20: #2 looking for paired plus/minus strand peaks... BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 18:38:21: 23000000 INFO @ Sun, 21 Jun 2020 18:38:22: #2 number of paired peaks: 4 WARNING @ Sun, 21 Jun 2020 18:38:22: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:38:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX1847049/SRX1847049.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1847049/SRX1847049.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1847049/SRX1847049.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1847049/SRX1847049.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 18:38:27: 24000000 INFO @ Sun, 21 Jun 2020 18:38:32: 25000000 INFO @ Sun, 21 Jun 2020 18:38:37: 26000000 INFO @ Sun, 21 Jun 2020 18:38:43: 27000000 INFO @ Sun, 21 Jun 2020 18:38:47: #1 tag size is determined as 49 bps INFO @ Sun, 21 Jun 2020 18:38:47: #1 tag size = 49 INFO @ Sun, 21 Jun 2020 18:38:47: #1 total tags in treatment: 27744575 INFO @ Sun, 21 Jun 2020 18:38:47: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:38:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:38:48: #1 tags after filtering in treatment: 27744575 INFO @ Sun, 21 Jun 2020 18:38:48: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:38:48: #1 finished! INFO @ Sun, 21 Jun 2020 18:38:48: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:38:48: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:38:49: #2 number of paired peaks: 4 WARNING @ Sun, 21 Jun 2020 18:38:49: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:38:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX1847049/SRX1847049.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1847049/SRX1847049.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1847049/SRX1847049.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1847049/SRX1847049.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。