Job ID = 6454167 SRX = SRX1847046 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T08:56:49 prefetch.2.10.7: 1) Downloading 'SRR3669863'... 2020-06-21T08:56:49 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T09:04:39 prefetch.2.10.7: HTTPS download succeed 2020-06-21T09:04:39 prefetch.2.10.7: 1) 'SRR3669863' was downloaded successfully Read 72621077 spots for SRR3669863/SRR3669863.sra Written 72621077 spots for SRR3669863/SRR3669863.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:24:56 72621077 reads; of these: 72621077 (100.00%) were unpaired; of these: 9216458 (12.69%) aligned 0 times 45963976 (63.29%) aligned exactly 1 time 17440643 (24.02%) aligned >1 times 87.31% overall alignment rate Time searching: 00:24:56 Overall time: 00:24:56 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 28 files... [bam_rmdupse_core] 26490983 / 63404619 = 0.4178 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:48:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX1847046/SRX1847046.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX1847046/SRX1847046.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX1847046/SRX1847046.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX1847046/SRX1847046.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:48:18: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:48:18: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:48:23: 1000000 INFO @ Sun, 21 Jun 2020 18:48:29: 2000000 INFO @ Sun, 21 Jun 2020 18:48:34: 3000000 INFO @ Sun, 21 Jun 2020 18:48:39: 4000000 INFO @ Sun, 21 Jun 2020 18:48:44: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:48:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX1847046/SRX1847046.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX1847046/SRX1847046.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX1847046/SRX1847046.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX1847046/SRX1847046.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:48:48: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:48:48: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:48:49: 6000000 INFO @ Sun, 21 Jun 2020 18:48:53: 1000000 INFO @ Sun, 21 Jun 2020 18:48:54: 7000000 INFO @ Sun, 21 Jun 2020 18:48:59: 2000000 INFO @ Sun, 21 Jun 2020 18:48:59: 8000000 INFO @ Sun, 21 Jun 2020 18:49:04: 3000000 INFO @ Sun, 21 Jun 2020 18:49:04: 9000000 INFO @ Sun, 21 Jun 2020 18:49:09: 4000000 INFO @ Sun, 21 Jun 2020 18:49:10: 10000000 INFO @ Sun, 21 Jun 2020 18:49:15: 5000000 INFO @ Sun, 21 Jun 2020 18:49:15: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:49:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX1847046/SRX1847046.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX1847046/SRX1847046.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX1847046/SRX1847046.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX1847046/SRX1847046.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:49:18: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:49:18: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:49:20: 6000000 INFO @ Sun, 21 Jun 2020 18:49:21: 12000000 INFO @ Sun, 21 Jun 2020 18:49:25: 1000000 INFO @ Sun, 21 Jun 2020 18:49:25: 7000000 INFO @ Sun, 21 Jun 2020 18:49:26: 13000000 INFO @ Sun, 21 Jun 2020 18:49:31: 8000000 INFO @ Sun, 21 Jun 2020 18:49:31: 2000000 INFO @ Sun, 21 Jun 2020 18:49:32: 14000000 INFO @ Sun, 21 Jun 2020 18:49:36: 9000000 INFO @ Sun, 21 Jun 2020 18:49:37: 15000000 INFO @ Sun, 21 Jun 2020 18:49:37: 3000000 INFO @ Sun, 21 Jun 2020 18:49:42: 10000000 INFO @ Sun, 21 Jun 2020 18:49:42: 16000000 INFO @ Sun, 21 Jun 2020 18:49:44: 4000000 INFO @ Sun, 21 Jun 2020 18:49:47: 11000000 INFO @ Sun, 21 Jun 2020 18:49:48: 17000000 INFO @ Sun, 21 Jun 2020 18:49:50: 5000000 INFO @ Sun, 21 Jun 2020 18:49:53: 12000000 INFO @ Sun, 21 Jun 2020 18:49:54: 18000000 INFO @ Sun, 21 Jun 2020 18:49:56: 6000000 INFO @ Sun, 21 Jun 2020 18:49:59: 13000000 INFO @ Sun, 21 Jun 2020 18:49:59: 19000000 INFO @ Sun, 21 Jun 2020 18:50:02: 7000000 INFO @ Sun, 21 Jun 2020 18:50:04: 14000000 INFO @ Sun, 21 Jun 2020 18:50:05: 20000000 INFO @ Sun, 21 Jun 2020 18:50:09: 8000000 INFO @ Sun, 21 Jun 2020 18:50:10: 15000000 INFO @ Sun, 21 Jun 2020 18:50:11: 21000000 INFO @ Sun, 21 Jun 2020 18:50:15: 9000000 INFO @ Sun, 21 Jun 2020 18:50:15: 16000000 INFO @ Sun, 21 Jun 2020 18:50:16: 22000000 INFO @ Sun, 21 Jun 2020 18:50:21: 17000000 INFO @ Sun, 21 Jun 2020 18:50:21: 10000000 INFO @ Sun, 21 Jun 2020 18:50:22: 23000000 INFO @ Sun, 21 Jun 2020 18:50:26: 18000000 INFO @ Sun, 21 Jun 2020 18:50:27: 24000000 INFO @ Sun, 21 Jun 2020 18:50:27: 11000000 INFO @ Sun, 21 Jun 2020 18:50:32: 19000000 INFO @ Sun, 21 Jun 2020 18:50:33: 25000000 INFO @ Sun, 21 Jun 2020 18:50:34: 12000000 INFO @ Sun, 21 Jun 2020 18:50:37: 20000000 INFO @ Sun, 21 Jun 2020 18:50:39: 26000000 INFO @ Sun, 21 Jun 2020 18:50:40: 13000000 INFO @ Sun, 21 Jun 2020 18:50:43: 21000000 INFO @ Sun, 21 Jun 2020 18:50:44: 27000000 INFO @ Sun, 21 Jun 2020 18:50:46: 14000000 INFO @ Sun, 21 Jun 2020 18:50:49: 22000000 INFO @ Sun, 21 Jun 2020 18:50:50: 28000000 INFO @ Sun, 21 Jun 2020 18:50:52: 15000000 INFO @ Sun, 21 Jun 2020 18:50:54: 23000000 INFO @ Sun, 21 Jun 2020 18:50:56: 29000000 INFO @ Sun, 21 Jun 2020 18:50:58: 16000000 INFO @ Sun, 21 Jun 2020 18:51:00: 24000000 INFO @ Sun, 21 Jun 2020 18:51:02: 30000000 INFO @ Sun, 21 Jun 2020 18:51:05: 17000000 INFO @ Sun, 21 Jun 2020 18:51:06: 25000000 INFO @ Sun, 21 Jun 2020 18:51:08: 31000000 INFO @ Sun, 21 Jun 2020 18:51:11: 18000000 INFO @ Sun, 21 Jun 2020 18:51:11: 26000000 INFO @ Sun, 21 Jun 2020 18:51:13: 32000000 INFO @ Sun, 21 Jun 2020 18:51:17: 19000000 INFO @ Sun, 21 Jun 2020 18:51:17: 27000000 INFO @ Sun, 21 Jun 2020 18:51:19: 33000000 INFO @ Sun, 21 Jun 2020 18:51:23: 28000000 INFO @ Sun, 21 Jun 2020 18:51:23: 20000000 INFO @ Sun, 21 Jun 2020 18:51:25: 34000000 INFO @ Sun, 21 Jun 2020 18:51:29: 29000000 INFO @ Sun, 21 Jun 2020 18:51:30: 21000000 INFO @ Sun, 21 Jun 2020 18:51:31: 35000000 INFO @ Sun, 21 Jun 2020 18:51:35: 30000000 INFO @ Sun, 21 Jun 2020 18:51:36: 22000000 INFO @ Sun, 21 Jun 2020 18:51:36: 36000000 INFO @ Sun, 21 Jun 2020 18:51:40: 31000000 INFO @ Sun, 21 Jun 2020 18:51:42: #1 tag size is determined as 49 bps INFO @ Sun, 21 Jun 2020 18:51:42: #1 tag size = 49 INFO @ Sun, 21 Jun 2020 18:51:42: #1 total tags in treatment: 36913636 INFO @ Sun, 21 Jun 2020 18:51:42: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:51:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:51:42: 23000000 INFO @ Sun, 21 Jun 2020 18:51:43: #1 tags after filtering in treatment: 36913636 INFO @ Sun, 21 Jun 2020 18:51:43: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:51:43: #1 finished! INFO @ Sun, 21 Jun 2020 18:51:43: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:51:43: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:51:45: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 18:51:45: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:51:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX1847046/SRX1847046.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1847046/SRX1847046.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1847046/SRX1847046.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1847046/SRX1847046.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 18:51:46: 32000000 INFO @ Sun, 21 Jun 2020 18:51:48: 24000000 INFO @ Sun, 21 Jun 2020 18:51:52: 33000000 INFO @ Sun, 21 Jun 2020 18:51:55: 25000000 INFO @ Sun, 21 Jun 2020 18:51:57: 34000000 INFO @ Sun, 21 Jun 2020 18:52:01: 26000000 INFO @ Sun, 21 Jun 2020 18:52:03: 35000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 18:52:08: 27000000 INFO @ Sun, 21 Jun 2020 18:52:08: 36000000 INFO @ Sun, 21 Jun 2020 18:52:14: #1 tag size is determined as 49 bps INFO @ Sun, 21 Jun 2020 18:52:14: #1 tag size = 49 INFO @ Sun, 21 Jun 2020 18:52:14: #1 total tags in treatment: 36913636 INFO @ Sun, 21 Jun 2020 18:52:14: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:52:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:52:14: 28000000 INFO @ Sun, 21 Jun 2020 18:52:15: #1 tags after filtering in treatment: 36913636 INFO @ Sun, 21 Jun 2020 18:52:15: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:52:15: #1 finished! INFO @ Sun, 21 Jun 2020 18:52:15: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:52:15: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:52:17: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 18:52:17: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:52:17: Process for pairing-model is terminated! INFO @ Sun, 21 Jun 2020 18:52:20: 29000000 cut: /home/okishinya/chipatlas/results/dm6/SRX1847046/SRX1847046.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1847046/SRX1847046.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1847046/SRX1847046.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1847046/SRX1847046.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 18:52:27: 30000000 INFO @ Sun, 21 Jun 2020 18:52:33: 31000000 INFO @ Sun, 21 Jun 2020 18:52:39: 32000000 INFO @ Sun, 21 Jun 2020 18:52:45: 33000000 INFO @ Sun, 21 Jun 2020 18:52:51: 34000000 INFO @ Sun, 21 Jun 2020 18:52:57: 35000000 INFO @ Sun, 21 Jun 2020 18:53:04: 36000000 INFO @ Sun, 21 Jun 2020 18:53:10: #1 tag size is determined as 49 bps INFO @ Sun, 21 Jun 2020 18:53:10: #1 tag size = 49 INFO @ Sun, 21 Jun 2020 18:53:10: #1 total tags in treatment: 36913636 INFO @ Sun, 21 Jun 2020 18:53:10: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:53:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:53:11: #1 tags after filtering in treatment: 36913636 INFO @ Sun, 21 Jun 2020 18:53:11: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:53:11: #1 finished! INFO @ Sun, 21 Jun 2020 18:53:11: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:53:11: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:53:13: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 18:53:13: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:53:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX1847046/SRX1847046.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1847046/SRX1847046.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1847046/SRX1847046.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1847046/SRX1847046.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。