Job ID = 6453986 SRX = SRX1760694 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T08:49:34 prefetch.2.10.7: 1) Downloading 'SRR3503050'... 2020-06-21T08:49:34 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T08:51:13 prefetch.2.10.7: HTTPS download succeed 2020-06-21T08:51:14 prefetch.2.10.7: 'SRR3503050' is valid 2020-06-21T08:51:14 prefetch.2.10.7: 1) 'SRR3503050' was downloaded successfully 2020-06-21T08:51:14 prefetch.2.10.7: 'SRR3503050' has 0 unresolved dependencies Read 5025654 spots for SRR3503050/SRR3503050.sra Written 5025654 spots for SRR3503050/SRR3503050.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:25 5025654 reads; of these: 5025654 (100.00%) were unpaired; of these: 1822819 (36.27%) aligned 0 times 2423658 (48.23%) aligned exactly 1 time 779177 (15.50%) aligned >1 times 63.73% overall alignment rate Time searching: 00:04:25 Overall time: 00:04:25 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_rmdupse_core] 141100 / 3202835 = 0.0441 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:58:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX1760694/SRX1760694.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX1760694/SRX1760694.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX1760694/SRX1760694.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX1760694/SRX1760694.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:58:09: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:58:09: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:58:18: 1000000 INFO @ Sun, 21 Jun 2020 17:58:26: 2000000 INFO @ Sun, 21 Jun 2020 17:58:34: 3000000 INFO @ Sun, 21 Jun 2020 17:58:35: #1 tag size is determined as 151 bps INFO @ Sun, 21 Jun 2020 17:58:35: #1 tag size = 151 INFO @ Sun, 21 Jun 2020 17:58:35: #1 total tags in treatment: 3061735 INFO @ Sun, 21 Jun 2020 17:58:35: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:58:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:58:35: #1 tags after filtering in treatment: 3061451 INFO @ Sun, 21 Jun 2020 17:58:35: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:58:35: #1 finished! INFO @ Sun, 21 Jun 2020 17:58:35: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:58:35: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:58:35: #2 number of paired peaks: 850 WARNING @ Sun, 21 Jun 2020 17:58:35: Fewer paired peaks (850) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 850 pairs to build model! INFO @ Sun, 21 Jun 2020 17:58:35: start model_add_line... INFO @ Sun, 21 Jun 2020 17:58:35: start X-correlation... INFO @ Sun, 21 Jun 2020 17:58:35: end of X-cor INFO @ Sun, 21 Jun 2020 17:58:35: #2 finished! INFO @ Sun, 21 Jun 2020 17:58:35: #2 predicted fragment length is 141 bps INFO @ Sun, 21 Jun 2020 17:58:35: #2 alternative fragment length(s) may be 141 bps INFO @ Sun, 21 Jun 2020 17:58:35: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX1760694/SRX1760694.05_model.r WARNING @ Sun, 21 Jun 2020 17:58:35: #2 Since the d (141) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sun, 21 Jun 2020 17:58:35: #2 You may need to consider one of the other alternative d(s): 141 WARNING @ Sun, 21 Jun 2020 17:58:35: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sun, 21 Jun 2020 17:58:35: #3 Call peaks... INFO @ Sun, 21 Jun 2020 17:58:35: #3 Pre-compute pvalue-qvalue table... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:58:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX1760694/SRX1760694.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX1760694/SRX1760694.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX1760694/SRX1760694.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX1760694/SRX1760694.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:58:39: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:58:39: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:58:43: #3 Call peaks for each chromosome... INFO @ Sun, 21 Jun 2020 17:58:46: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX1760694/SRX1760694.05_peaks.xls INFO @ Sun, 21 Jun 2020 17:58:46: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX1760694/SRX1760694.05_peaks.narrowPeak INFO @ Sun, 21 Jun 2020 17:58:46: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX1760694/SRX1760694.05_summits.bed INFO @ Sun, 21 Jun 2020 17:58:46: Done! pass1 - making usageList (503 chroms): 1 millis pass2 - checking and writing primary data (1083 records, 4 fields): 13 millis CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 17:58:48: 1000000 INFO @ Sun, 21 Jun 2020 17:58:58: 2000000 INFO @ Sun, 21 Jun 2020 17:59:07: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 17:59:08: #1 tag size is determined as 151 bps INFO @ Sun, 21 Jun 2020 17:59:08: #1 tag size = 151 INFO @ Sun, 21 Jun 2020 17:59:08: #1 total tags in treatment: 3061735 INFO @ Sun, 21 Jun 2020 17:59:08: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:59:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:59:08: #1 tags after filtering in treatment: 3061451 INFO @ Sun, 21 Jun 2020 17:59:08: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:59:08: #1 finished! INFO @ Sun, 21 Jun 2020 17:59:08: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:59:08: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:59:09: #2 number of paired peaks: 850 WARNING @ Sun, 21 Jun 2020 17:59:09: Fewer paired peaks (850) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 850 pairs to build model! INFO @ Sun, 21 Jun 2020 17:59:09: start model_add_line... INFO @ Sun, 21 Jun 2020 17:59:09: start X-correlation... INFO @ Sun, 21 Jun 2020 17:59:09: end of X-cor INFO @ Sun, 21 Jun 2020 17:59:09: #2 finished! INFO @ Sun, 21 Jun 2020 17:59:09: #2 predicted fragment length is 141 bps INFO @ Sun, 21 Jun 2020 17:59:09: #2 alternative fragment length(s) may be 141 bps INFO @ Sun, 21 Jun 2020 17:59:09: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX1760694/SRX1760694.10_model.r WARNING @ Sun, 21 Jun 2020 17:59:09: #2 Since the d (141) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sun, 21 Jun 2020 17:59:09: #2 You may need to consider one of the other alternative d(s): 141 WARNING @ Sun, 21 Jun 2020 17:59:09: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sun, 21 Jun 2020 17:59:09: #3 Call peaks... INFO @ Sun, 21 Jun 2020 17:59:09: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 21 Jun 2020 17:59:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX1760694/SRX1760694.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX1760694/SRX1760694.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX1760694/SRX1760694.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX1760694/SRX1760694.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 17:59:09: #1 read tag files... INFO @ Sun, 21 Jun 2020 17:59:09: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 17:59:16: #3 Call peaks for each chromosome... INFO @ Sun, 21 Jun 2020 17:59:18: 1000000 INFO @ Sun, 21 Jun 2020 17:59:19: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX1760694/SRX1760694.10_peaks.xls INFO @ Sun, 21 Jun 2020 17:59:19: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX1760694/SRX1760694.10_peaks.narrowPeak INFO @ Sun, 21 Jun 2020 17:59:19: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX1760694/SRX1760694.10_summits.bed INFO @ Sun, 21 Jun 2020 17:59:19: Done! pass1 - making usageList (403 chroms): 1 millis pass2 - checking and writing primary data (730 records, 4 fields): 11 millis CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 17:59:27: 2000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 17:59:37: 3000000 INFO @ Sun, 21 Jun 2020 17:59:38: #1 tag size is determined as 151 bps INFO @ Sun, 21 Jun 2020 17:59:38: #1 tag size = 151 INFO @ Sun, 21 Jun 2020 17:59:38: #1 total tags in treatment: 3061735 INFO @ Sun, 21 Jun 2020 17:59:38: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 17:59:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 17:59:38: #1 tags after filtering in treatment: 3061451 INFO @ Sun, 21 Jun 2020 17:59:38: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 17:59:38: #1 finished! INFO @ Sun, 21 Jun 2020 17:59:38: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 17:59:38: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 17:59:38: #2 number of paired peaks: 850 WARNING @ Sun, 21 Jun 2020 17:59:38: Fewer paired peaks (850) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 850 pairs to build model! INFO @ Sun, 21 Jun 2020 17:59:38: start model_add_line... INFO @ Sun, 21 Jun 2020 17:59:38: start X-correlation... INFO @ Sun, 21 Jun 2020 17:59:38: end of X-cor INFO @ Sun, 21 Jun 2020 17:59:38: #2 finished! INFO @ Sun, 21 Jun 2020 17:59:38: #2 predicted fragment length is 141 bps INFO @ Sun, 21 Jun 2020 17:59:38: #2 alternative fragment length(s) may be 141 bps INFO @ Sun, 21 Jun 2020 17:59:38: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX1760694/SRX1760694.20_model.r WARNING @ Sun, 21 Jun 2020 17:59:38: #2 Since the d (141) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sun, 21 Jun 2020 17:59:38: #2 You may need to consider one of the other alternative d(s): 141 WARNING @ Sun, 21 Jun 2020 17:59:38: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sun, 21 Jun 2020 17:59:38: #3 Call peaks... INFO @ Sun, 21 Jun 2020 17:59:38: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Sun, 21 Jun 2020 17:59:45: #3 Call peaks for each chromosome... INFO @ Sun, 21 Jun 2020 17:59:48: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX1760694/SRX1760694.20_peaks.xls INFO @ Sun, 21 Jun 2020 17:59:48: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX1760694/SRX1760694.20_peaks.narrowPeak INFO @ Sun, 21 Jun 2020 17:59:48: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX1760694/SRX1760694.20_summits.bed INFO @ Sun, 21 Jun 2020 17:59:48: Done! pass1 - making usageList (273 chroms): 1 millis pass2 - checking and writing primary data (386 records, 4 fields): 10 millis CompletedMACS2peakCalling