Job ID = 6453968 SRX = SRX1715681 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T08:48:49 prefetch.2.10.7: 1) Downloading 'SRR3405222'... 2020-06-21T08:48:49 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T08:53:30 prefetch.2.10.7: HTTPS download succeed 2020-06-21T08:53:30 prefetch.2.10.7: 1) 'SRR3405222' was downloaded successfully Read 26463516 spots for SRR3405222/SRR3405222.sra Written 26463516 spots for SRR3405222/SRR3405222.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:07:11 26463516 reads; of these: 26463516 (100.00%) were unpaired; of these: 958466 (3.62%) aligned 0 times 20999656 (79.35%) aligned exactly 1 time 4505394 (17.02%) aligned >1 times 96.38% overall alignment rate Time searching: 00:07:12 Overall time: 00:07:12 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 7159713 / 25505050 = 0.2807 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:08:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX1715681/SRX1715681.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX1715681/SRX1715681.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX1715681/SRX1715681.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX1715681/SRX1715681.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:08:42: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:08:42: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:08:48: 1000000 INFO @ Sun, 21 Jun 2020 18:08:54: 2000000 INFO @ Sun, 21 Jun 2020 18:09:00: 3000000 INFO @ Sun, 21 Jun 2020 18:09:06: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:09:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX1715681/SRX1715681.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX1715681/SRX1715681.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX1715681/SRX1715681.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX1715681/SRX1715681.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:09:11: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:09:11: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:09:13: 5000000 INFO @ Sun, 21 Jun 2020 18:09:17: 1000000 INFO @ Sun, 21 Jun 2020 18:09:19: 6000000 INFO @ Sun, 21 Jun 2020 18:09:23: 2000000 INFO @ Sun, 21 Jun 2020 18:09:26: 7000000 INFO @ Sun, 21 Jun 2020 18:09:30: 3000000 INFO @ Sun, 21 Jun 2020 18:09:32: 8000000 INFO @ Sun, 21 Jun 2020 18:09:35: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:09:39: 9000000 INFO @ Sun, 21 Jun 2020 18:09:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX1715681/SRX1715681.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX1715681/SRX1715681.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX1715681/SRX1715681.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX1715681/SRX1715681.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:09:41: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:09:41: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:09:41: 5000000 INFO @ Sun, 21 Jun 2020 18:09:46: 10000000 INFO @ Sun, 21 Jun 2020 18:09:46: 1000000 INFO @ Sun, 21 Jun 2020 18:09:47: 6000000 INFO @ Sun, 21 Jun 2020 18:09:52: 2000000 INFO @ Sun, 21 Jun 2020 18:09:52: 11000000 INFO @ Sun, 21 Jun 2020 18:09:53: 7000000 INFO @ Sun, 21 Jun 2020 18:09:58: 3000000 INFO @ Sun, 21 Jun 2020 18:09:58: 8000000 INFO @ Sun, 21 Jun 2020 18:09:59: 12000000 INFO @ Sun, 21 Jun 2020 18:10:04: 4000000 INFO @ Sun, 21 Jun 2020 18:10:04: 9000000 INFO @ Sun, 21 Jun 2020 18:10:05: 13000000 INFO @ Sun, 21 Jun 2020 18:10:09: 5000000 INFO @ Sun, 21 Jun 2020 18:10:10: 10000000 INFO @ Sun, 21 Jun 2020 18:10:12: 14000000 INFO @ Sun, 21 Jun 2020 18:10:15: 6000000 INFO @ Sun, 21 Jun 2020 18:10:16: 11000000 INFO @ Sun, 21 Jun 2020 18:10:19: 15000000 INFO @ Sun, 21 Jun 2020 18:10:21: 7000000 INFO @ Sun, 21 Jun 2020 18:10:21: 12000000 INFO @ Sun, 21 Jun 2020 18:10:25: 16000000 INFO @ Sun, 21 Jun 2020 18:10:27: 8000000 INFO @ Sun, 21 Jun 2020 18:10:27: 13000000 INFO @ Sun, 21 Jun 2020 18:10:32: 17000000 INFO @ Sun, 21 Jun 2020 18:10:33: 14000000 INFO @ Sun, 21 Jun 2020 18:10:33: 9000000 INFO @ Sun, 21 Jun 2020 18:10:38: 18000000 INFO @ Sun, 21 Jun 2020 18:10:38: 10000000 INFO @ Sun, 21 Jun 2020 18:10:39: 15000000 INFO @ Sun, 21 Jun 2020 18:10:41: #1 tag size is determined as 49 bps INFO @ Sun, 21 Jun 2020 18:10:41: #1 tag size = 49 INFO @ Sun, 21 Jun 2020 18:10:41: #1 total tags in treatment: 18345337 INFO @ Sun, 21 Jun 2020 18:10:41: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:10:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:10:41: #1 tags after filtering in treatment: 18345337 INFO @ Sun, 21 Jun 2020 18:10:41: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:10:41: #1 finished! INFO @ Sun, 21 Jun 2020 18:10:41: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:10:41: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:10:43: #2 number of paired peaks: 75 WARNING @ Sun, 21 Jun 2020 18:10:43: Too few paired peaks (75) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:10:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX1715681/SRX1715681.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1715681/SRX1715681.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1715681/SRX1715681.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1715681/SRX1715681.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 18:10:44: 16000000 INFO @ Sun, 21 Jun 2020 18:10:44: 11000000 INFO @ Sun, 21 Jun 2020 18:10:50: 17000000 INFO @ Sun, 21 Jun 2020 18:10:50: 12000000 INFO @ Sun, 21 Jun 2020 18:10:55: 18000000 INFO @ Sun, 21 Jun 2020 18:10:55: 13000000 INFO @ Sun, 21 Jun 2020 18:10:57: #1 tag size is determined as 49 bps INFO @ Sun, 21 Jun 2020 18:10:57: #1 tag size = 49 INFO @ Sun, 21 Jun 2020 18:10:57: #1 total tags in treatment: 18345337 INFO @ Sun, 21 Jun 2020 18:10:57: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:10:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:10:58: #1 tags after filtering in treatment: 18345337 INFO @ Sun, 21 Jun 2020 18:10:58: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:10:58: #1 finished! INFO @ Sun, 21 Jun 2020 18:10:58: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:10:58: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:10:59: #2 number of paired peaks: 75 WARNING @ Sun, 21 Jun 2020 18:10:59: Too few paired peaks (75) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:10:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX1715681/SRX1715681.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1715681/SRX1715681.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1715681/SRX1715681.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1715681/SRX1715681.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 18:11:01: 14000000 INFO @ Sun, 21 Jun 2020 18:11:07: 15000000 INFO @ Sun, 21 Jun 2020 18:11:12: 16000000 INFO @ Sun, 21 Jun 2020 18:11:18: 17000000 INFO @ Sun, 21 Jun 2020 18:11:23: 18000000 INFO @ Sun, 21 Jun 2020 18:11:25: #1 tag size is determined as 49 bps INFO @ Sun, 21 Jun 2020 18:11:25: #1 tag size = 49 INFO @ Sun, 21 Jun 2020 18:11:25: #1 total tags in treatment: 18345337 INFO @ Sun, 21 Jun 2020 18:11:25: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:11:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:11:26: #1 tags after filtering in treatment: 18345337 INFO @ Sun, 21 Jun 2020 18:11:26: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:11:26: #1 finished! INFO @ Sun, 21 Jun 2020 18:11:26: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:11:26: #2 looking for paired plus/minus strand peaks... BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 18:11:27: #2 number of paired peaks: 75 WARNING @ Sun, 21 Jun 2020 18:11:27: Too few paired peaks (75) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:11:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX1715681/SRX1715681.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1715681/SRX1715681.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1715681/SRX1715681.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1715681/SRX1715681.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。