Job ID = 6453967 SRX = SRX1715680 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T08:51:49 prefetch.2.10.7: 1) Downloading 'SRR3405221'... 2020-06-21T08:51:49 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T08:58:47 prefetch.2.10.7: HTTPS download succeed 2020-06-21T08:58:47 prefetch.2.10.7: 1) 'SRR3405221' was downloaded successfully Read 51652207 spots for SRR3405221/SRR3405221.sra Written 51652207 spots for SRR3405221/SRR3405221.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:32 51652207 reads; of these: 51652207 (100.00%) were unpaired; of these: 1301795 (2.52%) aligned 0 times 44216417 (85.60%) aligned exactly 1 time 6133995 (11.88%) aligned >1 times 97.48% overall alignment rate Time searching: 00:11:32 Overall time: 00:11:32 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 18824452 / 50350412 = 0.3739 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:24:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX1715680/SRX1715680.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX1715680/SRX1715680.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX1715680/SRX1715680.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX1715680/SRX1715680.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:24:00: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:24:00: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:24:07: 1000000 INFO @ Sun, 21 Jun 2020 18:24:14: 2000000 INFO @ Sun, 21 Jun 2020 18:24:21: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:24:28: 4000000 INFO @ Sun, 21 Jun 2020 18:24:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX1715680/SRX1715680.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX1715680/SRX1715680.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX1715680/SRX1715680.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX1715680/SRX1715680.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:24:29: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:24:29: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:24:35: 5000000 INFO @ Sun, 21 Jun 2020 18:24:36: 1000000 INFO @ Sun, 21 Jun 2020 18:24:42: 6000000 INFO @ Sun, 21 Jun 2020 18:24:43: 2000000 INFO @ Sun, 21 Jun 2020 18:24:49: 7000000 INFO @ Sun, 21 Jun 2020 18:24:50: 3000000 INFO @ Sun, 21 Jun 2020 18:24:56: 8000000 INFO @ Sun, 21 Jun 2020 18:24:57: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:24:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX1715680/SRX1715680.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX1715680/SRX1715680.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX1715680/SRX1715680.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX1715680/SRX1715680.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:24:59: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:24:59: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:25:03: 9000000 INFO @ Sun, 21 Jun 2020 18:25:05: 5000000 INFO @ Sun, 21 Jun 2020 18:25:06: 1000000 INFO @ Sun, 21 Jun 2020 18:25:11: 10000000 INFO @ Sun, 21 Jun 2020 18:25:12: 6000000 INFO @ Sun, 21 Jun 2020 18:25:13: 2000000 INFO @ Sun, 21 Jun 2020 18:25:19: 11000000 INFO @ Sun, 21 Jun 2020 18:25:20: 7000000 INFO @ Sun, 21 Jun 2020 18:25:20: 3000000 INFO @ Sun, 21 Jun 2020 18:25:27: 12000000 INFO @ Sun, 21 Jun 2020 18:25:28: 4000000 INFO @ Sun, 21 Jun 2020 18:25:28: 8000000 INFO @ Sun, 21 Jun 2020 18:25:34: 13000000 INFO @ Sun, 21 Jun 2020 18:25:35: 5000000 INFO @ Sun, 21 Jun 2020 18:25:36: 9000000 INFO @ Sun, 21 Jun 2020 18:25:42: 6000000 INFO @ Sun, 21 Jun 2020 18:25:42: 14000000 INFO @ Sun, 21 Jun 2020 18:25:44: 10000000 INFO @ Sun, 21 Jun 2020 18:25:49: 7000000 INFO @ Sun, 21 Jun 2020 18:25:50: 15000000 INFO @ Sun, 21 Jun 2020 18:25:52: 11000000 INFO @ Sun, 21 Jun 2020 18:25:56: 8000000 INFO @ Sun, 21 Jun 2020 18:25:58: 16000000 INFO @ Sun, 21 Jun 2020 18:26:00: 12000000 INFO @ Sun, 21 Jun 2020 18:26:03: 9000000 INFO @ Sun, 21 Jun 2020 18:26:06: 17000000 INFO @ Sun, 21 Jun 2020 18:26:07: 13000000 INFO @ Sun, 21 Jun 2020 18:26:10: 10000000 INFO @ Sun, 21 Jun 2020 18:26:14: 18000000 INFO @ Sun, 21 Jun 2020 18:26:15: 14000000 INFO @ Sun, 21 Jun 2020 18:26:18: 11000000 INFO @ Sun, 21 Jun 2020 18:26:22: 19000000 INFO @ Sun, 21 Jun 2020 18:26:23: 15000000 INFO @ Sun, 21 Jun 2020 18:26:25: 12000000 INFO @ Sun, 21 Jun 2020 18:26:30: 20000000 INFO @ Sun, 21 Jun 2020 18:26:31: 16000000 INFO @ Sun, 21 Jun 2020 18:26:32: 13000000 INFO @ Sun, 21 Jun 2020 18:26:38: 21000000 INFO @ Sun, 21 Jun 2020 18:26:39: 17000000 INFO @ Sun, 21 Jun 2020 18:26:40: 14000000 INFO @ Sun, 21 Jun 2020 18:26:46: 22000000 INFO @ Sun, 21 Jun 2020 18:26:47: 15000000 INFO @ Sun, 21 Jun 2020 18:26:47: 18000000 INFO @ Sun, 21 Jun 2020 18:26:54: 16000000 INFO @ Sun, 21 Jun 2020 18:26:54: 23000000 INFO @ Sun, 21 Jun 2020 18:26:55: 19000000 INFO @ Sun, 21 Jun 2020 18:27:01: 17000000 INFO @ Sun, 21 Jun 2020 18:27:02: 24000000 INFO @ Sun, 21 Jun 2020 18:27:03: 20000000 INFO @ Sun, 21 Jun 2020 18:27:08: 18000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 18:27:10: 25000000 INFO @ Sun, 21 Jun 2020 18:27:11: 21000000 INFO @ Sun, 21 Jun 2020 18:27:15: 19000000 INFO @ Sun, 21 Jun 2020 18:27:18: 26000000 INFO @ Sun, 21 Jun 2020 18:27:18: 22000000 INFO @ Sun, 21 Jun 2020 18:27:22: 20000000 INFO @ Sun, 21 Jun 2020 18:27:26: 27000000 INFO @ Sun, 21 Jun 2020 18:27:26: 23000000 INFO @ Sun, 21 Jun 2020 18:27:29: 21000000 INFO @ Sun, 21 Jun 2020 18:27:34: 28000000 INFO @ Sun, 21 Jun 2020 18:27:34: 24000000 INFO @ Sun, 21 Jun 2020 18:27:36: 22000000 INFO @ Sun, 21 Jun 2020 18:27:41: 29000000 INFO @ Sun, 21 Jun 2020 18:27:41: 25000000 INFO @ Sun, 21 Jun 2020 18:27:43: 23000000 INFO @ Sun, 21 Jun 2020 18:27:49: 30000000 INFO @ Sun, 21 Jun 2020 18:27:49: 26000000 INFO @ Sun, 21 Jun 2020 18:27:50: 24000000 INFO @ Sun, 21 Jun 2020 18:27:57: 31000000 INFO @ Sun, 21 Jun 2020 18:27:57: 27000000 INFO @ Sun, 21 Jun 2020 18:27:57: 25000000 INFO @ Sun, 21 Jun 2020 18:28:01: #1 tag size is determined as 49 bps INFO @ Sun, 21 Jun 2020 18:28:01: #1 tag size = 49 INFO @ Sun, 21 Jun 2020 18:28:01: #1 total tags in treatment: 31525960 INFO @ Sun, 21 Jun 2020 18:28:01: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:28:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:28:02: #1 tags after filtering in treatment: 31525960 INFO @ Sun, 21 Jun 2020 18:28:02: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:28:02: #1 finished! INFO @ Sun, 21 Jun 2020 18:28:02: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:28:02: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:28:04: #2 number of paired peaks: 4 WARNING @ Sun, 21 Jun 2020 18:28:04: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:28:04: Process for pairing-model is terminated! INFO @ Sun, 21 Jun 2020 18:28:04: 26000000 cut: /home/okishinya/chipatlas/results/dm6/SRX1715680/SRX1715680.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1715680/SRX1715680.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1715680/SRX1715680.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1715680/SRX1715680.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 18:28:04: 28000000 INFO @ Sun, 21 Jun 2020 18:28:11: 27000000 INFO @ Sun, 21 Jun 2020 18:28:11: 29000000 BigWig に変換しました。 INFO @ Sun, 21 Jun 2020 18:28:18: 28000000 INFO @ Sun, 21 Jun 2020 18:28:19: 30000000 INFO @ Sun, 21 Jun 2020 18:28:24: 29000000 INFO @ Sun, 21 Jun 2020 18:28:27: 31000000 INFO @ Sun, 21 Jun 2020 18:28:31: #1 tag size is determined as 49 bps INFO @ Sun, 21 Jun 2020 18:28:31: #1 tag size = 49 INFO @ Sun, 21 Jun 2020 18:28:31: #1 total tags in treatment: 31525960 INFO @ Sun, 21 Jun 2020 18:28:31: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:28:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:28:31: 30000000 INFO @ Sun, 21 Jun 2020 18:28:32: #1 tags after filtering in treatment: 31525960 INFO @ Sun, 21 Jun 2020 18:28:32: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:28:32: #1 finished! INFO @ Sun, 21 Jun 2020 18:28:32: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:28:32: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:28:34: #2 number of paired peaks: 4 WARNING @ Sun, 21 Jun 2020 18:28:34: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:28:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX1715680/SRX1715680.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1715680/SRX1715680.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1715680/SRX1715680.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1715680/SRX1715680.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 18:28:38: 31000000 INFO @ Sun, 21 Jun 2020 18:28:41: #1 tag size is determined as 49 bps INFO @ Sun, 21 Jun 2020 18:28:41: #1 tag size = 49 INFO @ Sun, 21 Jun 2020 18:28:41: #1 total tags in treatment: 31525960 INFO @ Sun, 21 Jun 2020 18:28:41: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:28:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:28:42: #1 tags after filtering in treatment: 31525960 INFO @ Sun, 21 Jun 2020 18:28:42: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:28:42: #1 finished! INFO @ Sun, 21 Jun 2020 18:28:42: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:28:42: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:28:44: #2 number of paired peaks: 4 WARNING @ Sun, 21 Jun 2020 18:28:44: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:28:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX1715680/SRX1715680.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1715680/SRX1715680.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1715680/SRX1715680.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1715680/SRX1715680.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling