Job ID = 6453965 SRX = SRX1715678 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T08:45:47 prefetch.2.10.7: 1) Downloading 'SRR3405219'... 2020-06-21T08:45:47 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T09:04:49 prefetch.2.10.7: HTTPS download succeed 2020-06-21T09:04:49 prefetch.2.10.7: 1) 'SRR3405219' was downloaded successfully Read 49816806 spots for SRR3405219/SRR3405219.sra Written 49816806 spots for SRR3405219/SRR3405219.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:01 49816806 reads; of these: 49816806 (100.00%) were unpaired; of these: 1104897 (2.22%) aligned 0 times 42380651 (85.07%) aligned exactly 1 time 6331258 (12.71%) aligned >1 times 97.78% overall alignment rate Time searching: 00:12:01 Overall time: 00:12:01 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 19286356 / 48711909 = 0.3959 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:30:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX1715678/SRX1715678.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX1715678/SRX1715678.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX1715678/SRX1715678.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX1715678/SRX1715678.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:30:15: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:30:15: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:30:22: 1000000 INFO @ Sun, 21 Jun 2020 18:30:28: 2000000 INFO @ Sun, 21 Jun 2020 18:30:34: 3000000 INFO @ Sun, 21 Jun 2020 18:30:40: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:30:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX1715678/SRX1715678.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX1715678/SRX1715678.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX1715678/SRX1715678.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX1715678/SRX1715678.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:30:45: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:30:45: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:30:47: 5000000 INFO @ Sun, 21 Jun 2020 18:30:52: 1000000 INFO @ Sun, 21 Jun 2020 18:30:53: 6000000 INFO @ Sun, 21 Jun 2020 18:30:58: 2000000 INFO @ Sun, 21 Jun 2020 18:31:00: 7000000 INFO @ Sun, 21 Jun 2020 18:31:05: 3000000 INFO @ Sun, 21 Jun 2020 18:31:07: 8000000 INFO @ Sun, 21 Jun 2020 18:31:11: 4000000 INFO @ Sun, 21 Jun 2020 18:31:13: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:31:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX1715678/SRX1715678.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX1715678/SRX1715678.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX1715678/SRX1715678.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX1715678/SRX1715678.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:31:15: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:31:15: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:31:18: 5000000 INFO @ Sun, 21 Jun 2020 18:31:20: 10000000 INFO @ Sun, 21 Jun 2020 18:31:22: 1000000 INFO @ Sun, 21 Jun 2020 18:31:24: 6000000 INFO @ Sun, 21 Jun 2020 18:31:26: 11000000 INFO @ Sun, 21 Jun 2020 18:31:28: 2000000 INFO @ Sun, 21 Jun 2020 18:31:31: 7000000 INFO @ Sun, 21 Jun 2020 18:31:33: 12000000 INFO @ Sun, 21 Jun 2020 18:31:35: 3000000 INFO @ Sun, 21 Jun 2020 18:31:37: 8000000 INFO @ Sun, 21 Jun 2020 18:31:40: 13000000 INFO @ Sun, 21 Jun 2020 18:31:41: 4000000 INFO @ Sun, 21 Jun 2020 18:31:44: 9000000 INFO @ Sun, 21 Jun 2020 18:31:47: 14000000 INFO @ Sun, 21 Jun 2020 18:31:47: 5000000 INFO @ Sun, 21 Jun 2020 18:31:50: 10000000 INFO @ Sun, 21 Jun 2020 18:31:53: 6000000 INFO @ Sun, 21 Jun 2020 18:31:54: 15000000 INFO @ Sun, 21 Jun 2020 18:31:56: 11000000 INFO @ Sun, 21 Jun 2020 18:32:00: 7000000 INFO @ Sun, 21 Jun 2020 18:32:00: 16000000 INFO @ Sun, 21 Jun 2020 18:32:02: 12000000 INFO @ Sun, 21 Jun 2020 18:32:06: 8000000 INFO @ Sun, 21 Jun 2020 18:32:07: 17000000 INFO @ Sun, 21 Jun 2020 18:32:09: 13000000 INFO @ Sun, 21 Jun 2020 18:32:12: 9000000 INFO @ Sun, 21 Jun 2020 18:32:13: 18000000 INFO @ Sun, 21 Jun 2020 18:32:15: 14000000 INFO @ Sun, 21 Jun 2020 18:32:19: 10000000 INFO @ Sun, 21 Jun 2020 18:32:20: 19000000 INFO @ Sun, 21 Jun 2020 18:32:21: 15000000 INFO @ Sun, 21 Jun 2020 18:32:25: 11000000 INFO @ Sun, 21 Jun 2020 18:32:26: 20000000 INFO @ Sun, 21 Jun 2020 18:32:27: 16000000 INFO @ Sun, 21 Jun 2020 18:32:31: 12000000 INFO @ Sun, 21 Jun 2020 18:32:33: 21000000 INFO @ Sun, 21 Jun 2020 18:32:33: 17000000 INFO @ Sun, 21 Jun 2020 18:32:37: 13000000 INFO @ Sun, 21 Jun 2020 18:32:39: 18000000 INFO @ Sun, 21 Jun 2020 18:32:39: 22000000 INFO @ Sun, 21 Jun 2020 18:32:43: 14000000 INFO @ Sun, 21 Jun 2020 18:32:45: 19000000 INFO @ Sun, 21 Jun 2020 18:32:46: 23000000 INFO @ Sun, 21 Jun 2020 18:32:49: 15000000 INFO @ Sun, 21 Jun 2020 18:32:51: 20000000 INFO @ Sun, 21 Jun 2020 18:32:52: 24000000 INFO @ Sun, 21 Jun 2020 18:32:55: 16000000 INFO @ Sun, 21 Jun 2020 18:32:57: 21000000 INFO @ Sun, 21 Jun 2020 18:32:59: 25000000 INFO @ Sun, 21 Jun 2020 18:33:01: 17000000 INFO @ Sun, 21 Jun 2020 18:33:03: 22000000 INFO @ Sun, 21 Jun 2020 18:33:05: 26000000 INFO @ Sun, 21 Jun 2020 18:33:07: 18000000 INFO @ Sun, 21 Jun 2020 18:33:09: 23000000 INFO @ Sun, 21 Jun 2020 18:33:12: 27000000 INFO @ Sun, 21 Jun 2020 18:33:13: 19000000 INFO @ Sun, 21 Jun 2020 18:33:15: 24000000 INFO @ Sun, 21 Jun 2020 18:33:18: 28000000 INFO @ Sun, 21 Jun 2020 18:33:19: 20000000 INFO @ Sun, 21 Jun 2020 18:33:21: 25000000 INFO @ Sun, 21 Jun 2020 18:33:25: 21000000 INFO @ Sun, 21 Jun 2020 18:33:25: 29000000 INFO @ Sun, 21 Jun 2020 18:33:27: 26000000 INFO @ Sun, 21 Jun 2020 18:33:28: #1 tag size is determined as 49 bps INFO @ Sun, 21 Jun 2020 18:33:28: #1 tag size = 49 INFO @ Sun, 21 Jun 2020 18:33:28: #1 total tags in treatment: 29425553 INFO @ Sun, 21 Jun 2020 18:33:28: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:33:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:33:29: #1 tags after filtering in treatment: 29425552 INFO @ Sun, 21 Jun 2020 18:33:29: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:33:29: #1 finished! INFO @ Sun, 21 Jun 2020 18:33:29: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:33:29: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:33:30: 22000000 INFO @ Sun, 21 Jun 2020 18:33:31: #2 number of paired peaks: 3 WARNING @ Sun, 21 Jun 2020 18:33:31: Too few paired peaks (3) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:33:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX1715678/SRX1715678.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1715678/SRX1715678.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1715678/SRX1715678.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1715678/SRX1715678.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 18:33:33: 27000000 INFO @ Sun, 21 Jun 2020 18:33:36: 23000000 INFO @ Sun, 21 Jun 2020 18:33:38: 28000000 INFO @ Sun, 21 Jun 2020 18:33:42: 24000000 INFO @ Sun, 21 Jun 2020 18:33:44: 29000000 INFO @ Sun, 21 Jun 2020 18:33:47: #1 tag size is determined as 49 bps INFO @ Sun, 21 Jun 2020 18:33:47: #1 tag size = 49 INFO @ Sun, 21 Jun 2020 18:33:47: #1 total tags in treatment: 29425553 INFO @ Sun, 21 Jun 2020 18:33:47: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:33:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:33:48: 25000000 INFO @ Sun, 21 Jun 2020 18:33:48: #1 tags after filtering in treatment: 29425552 INFO @ Sun, 21 Jun 2020 18:33:48: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:33:48: #1 finished! INFO @ Sun, 21 Jun 2020 18:33:48: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:33:48: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:33:50: #2 number of paired peaks: 3 WARNING @ Sun, 21 Jun 2020 18:33:50: Too few paired peaks (3) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:33:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX1715678/SRX1715678.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1715678/SRX1715678.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1715678/SRX1715678.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1715678/SRX1715678.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 18:33:53: 26000000 INFO @ Sun, 21 Jun 2020 18:33:58: 27000000 INFO @ Sun, 21 Jun 2020 18:34:03: 28000000 INFO @ Sun, 21 Jun 2020 18:34:08: 29000000 INFO @ Sun, 21 Jun 2020 18:34:11: #1 tag size is determined as 49 bps INFO @ Sun, 21 Jun 2020 18:34:11: #1 tag size = 49 INFO @ Sun, 21 Jun 2020 18:34:11: #1 total tags in treatment: 29425553 INFO @ Sun, 21 Jun 2020 18:34:11: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:34:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:34:12: #1 tags after filtering in treatment: 29425552 INFO @ Sun, 21 Jun 2020 18:34:12: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:34:12: #1 finished! INFO @ Sun, 21 Jun 2020 18:34:12: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:34:12: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:34:14: #2 number of paired peaks: 3 WARNING @ Sun, 21 Jun 2020 18:34:14: Too few paired peaks (3) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:34:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX1715678/SRX1715678.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1715678/SRX1715678.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1715678/SRX1715678.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1715678/SRX1715678.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。