Job ID = 6453964 SRX = SRX1715676 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T08:45:32 prefetch.2.10.7: 1) Downloading 'SRR3405217'... 2020-06-21T08:45:32 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T08:51:53 prefetch.2.10.7: HTTPS download succeed 2020-06-21T08:51:53 prefetch.2.10.7: 1) 'SRR3405217' was downloaded successfully Read 45198057 spots for SRR3405217/SRR3405217.sra Written 45198057 spots for SRR3405217/SRR3405217.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:50 45198057 reads; of these: 45198057 (100.00%) were unpaired; of these: 1512681 (3.35%) aligned 0 times 35923052 (79.48%) aligned exactly 1 time 7762324 (17.17%) aligned >1 times 96.65% overall alignment rate Time searching: 00:10:50 Overall time: 00:10:50 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 17199010 / 43685376 = 0.3937 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:16:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX1715676/SRX1715676.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX1715676/SRX1715676.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX1715676/SRX1715676.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX1715676/SRX1715676.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:16:34: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:16:34: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:16:39: 1000000 INFO @ Sun, 21 Jun 2020 18:16:44: 2000000 INFO @ Sun, 21 Jun 2020 18:16:49: 3000000 INFO @ Sun, 21 Jun 2020 18:16:53: 4000000 INFO @ Sun, 21 Jun 2020 18:16:58: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:17:03: 6000000 INFO @ Sun, 21 Jun 2020 18:17:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX1715676/SRX1715676.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX1715676/SRX1715676.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX1715676/SRX1715676.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX1715676/SRX1715676.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:17:04: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:17:04: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:17:08: 7000000 INFO @ Sun, 21 Jun 2020 18:17:09: 1000000 INFO @ Sun, 21 Jun 2020 18:17:12: 8000000 INFO @ Sun, 21 Jun 2020 18:17:14: 2000000 INFO @ Sun, 21 Jun 2020 18:17:17: 9000000 INFO @ Sun, 21 Jun 2020 18:17:19: 3000000 INFO @ Sun, 21 Jun 2020 18:17:22: 10000000 INFO @ Sun, 21 Jun 2020 18:17:24: 4000000 INFO @ Sun, 21 Jun 2020 18:17:26: 11000000 INFO @ Sun, 21 Jun 2020 18:17:29: 5000000 INFO @ Sun, 21 Jun 2020 18:17:31: 12000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:17:33: 6000000 INFO @ Sun, 21 Jun 2020 18:17:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX1715676/SRX1715676.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX1715676/SRX1715676.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX1715676/SRX1715676.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX1715676/SRX1715676.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:17:34: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:17:34: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:17:36: 13000000 INFO @ Sun, 21 Jun 2020 18:17:38: 7000000 INFO @ Sun, 21 Jun 2020 18:17:39: 1000000 INFO @ Sun, 21 Jun 2020 18:17:41: 14000000 INFO @ Sun, 21 Jun 2020 18:17:43: 8000000 INFO @ Sun, 21 Jun 2020 18:17:45: 2000000 INFO @ Sun, 21 Jun 2020 18:17:46: 15000000 INFO @ Sun, 21 Jun 2020 18:17:48: 9000000 INFO @ Sun, 21 Jun 2020 18:17:50: 3000000 INFO @ Sun, 21 Jun 2020 18:17:51: 16000000 INFO @ Sun, 21 Jun 2020 18:17:53: 10000000 INFO @ Sun, 21 Jun 2020 18:17:55: 4000000 INFO @ Sun, 21 Jun 2020 18:17:56: 17000000 INFO @ Sun, 21 Jun 2020 18:17:58: 11000000 INFO @ Sun, 21 Jun 2020 18:18:00: 5000000 INFO @ Sun, 21 Jun 2020 18:18:01: 18000000 INFO @ Sun, 21 Jun 2020 18:18:02: 12000000 INFO @ Sun, 21 Jun 2020 18:18:05: 6000000 INFO @ Sun, 21 Jun 2020 18:18:06: 19000000 INFO @ Sun, 21 Jun 2020 18:18:07: 13000000 INFO @ Sun, 21 Jun 2020 18:18:11: 7000000 INFO @ Sun, 21 Jun 2020 18:18:12: 20000000 INFO @ Sun, 21 Jun 2020 18:18:12: 14000000 INFO @ Sun, 21 Jun 2020 18:18:16: 8000000 INFO @ Sun, 21 Jun 2020 18:18:17: 21000000 INFO @ Sun, 21 Jun 2020 18:18:17: 15000000 INFO @ Sun, 21 Jun 2020 18:18:21: 9000000 INFO @ Sun, 21 Jun 2020 18:18:22: 16000000 INFO @ Sun, 21 Jun 2020 18:18:23: 22000000 INFO @ Sun, 21 Jun 2020 18:18:26: 10000000 INFO @ Sun, 21 Jun 2020 18:18:28: 17000000 INFO @ Sun, 21 Jun 2020 18:18:28: 23000000 INFO @ Sun, 21 Jun 2020 18:18:32: 11000000 INFO @ Sun, 21 Jun 2020 18:18:33: 18000000 INFO @ Sun, 21 Jun 2020 18:18:34: 24000000 INFO @ Sun, 21 Jun 2020 18:18:37: 12000000 INFO @ Sun, 21 Jun 2020 18:18:38: 19000000 INFO @ Sun, 21 Jun 2020 18:18:40: 25000000 INFO @ Sun, 21 Jun 2020 18:18:43: 20000000 INFO @ Sun, 21 Jun 2020 18:18:43: 13000000 INFO @ Sun, 21 Jun 2020 18:18:46: 26000000 INFO @ Sun, 21 Jun 2020 18:18:49: 21000000 INFO @ Sun, 21 Jun 2020 18:18:49: 14000000 INFO @ Sun, 21 Jun 2020 18:18:50: #1 tag size is determined as 49 bps INFO @ Sun, 21 Jun 2020 18:18:50: #1 tag size = 49 INFO @ Sun, 21 Jun 2020 18:18:50: #1 total tags in treatment: 26486366 INFO @ Sun, 21 Jun 2020 18:18:50: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:18:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:18:51: #1 tags after filtering in treatment: 26486366 INFO @ Sun, 21 Jun 2020 18:18:51: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:18:51: #1 finished! INFO @ Sun, 21 Jun 2020 18:18:51: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:18:51: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:18:52: #2 number of paired peaks: 17 WARNING @ Sun, 21 Jun 2020 18:18:52: Too few paired peaks (17) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:18:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX1715676/SRX1715676.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1715676/SRX1715676.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1715676/SRX1715676.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1715676/SRX1715676.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 18:18:55: 15000000 INFO @ Sun, 21 Jun 2020 18:18:56: 22000000 INFO @ Sun, 21 Jun 2020 18:19:00: 16000000 INFO @ Sun, 21 Jun 2020 18:19:01: 23000000 INFO @ Sun, 21 Jun 2020 18:19:06: 17000000 INFO @ Sun, 21 Jun 2020 18:19:07: 24000000 INFO @ Sun, 21 Jun 2020 18:19:12: 18000000 INFO @ Sun, 21 Jun 2020 18:19:13: 25000000 INFO @ Sun, 21 Jun 2020 18:19:18: 19000000 INFO @ Sun, 21 Jun 2020 18:19:19: 26000000 INFO @ Sun, 21 Jun 2020 18:19:23: #1 tag size is determined as 49 bps INFO @ Sun, 21 Jun 2020 18:19:23: #1 tag size = 49 INFO @ Sun, 21 Jun 2020 18:19:23: #1 total tags in treatment: 26486366 INFO @ Sun, 21 Jun 2020 18:19:23: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:19:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:19:24: #1 tags after filtering in treatment: 26486366 INFO @ Sun, 21 Jun 2020 18:19:24: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:19:24: #1 finished! INFO @ Sun, 21 Jun 2020 18:19:24: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:19:24: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:19:24: 20000000 INFO @ Sun, 21 Jun 2020 18:19:25: #2 number of paired peaks: 17 WARNING @ Sun, 21 Jun 2020 18:19:25: Too few paired peaks (17) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:19:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX1715676/SRX1715676.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1715676/SRX1715676.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1715676/SRX1715676.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1715676/SRX1715676.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 18:19:30: 21000000 INFO @ Sun, 21 Jun 2020 18:19:38: 22000000 INFO @ Sun, 21 Jun 2020 18:19:44: 23000000 INFO @ Sun, 21 Jun 2020 18:19:50: 24000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 18:19:56: 25000000 INFO @ Sun, 21 Jun 2020 18:20:02: 26000000 INFO @ Sun, 21 Jun 2020 18:20:06: #1 tag size is determined as 49 bps INFO @ Sun, 21 Jun 2020 18:20:06: #1 tag size = 49 INFO @ Sun, 21 Jun 2020 18:20:06: #1 total tags in treatment: 26486366 INFO @ Sun, 21 Jun 2020 18:20:06: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:20:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:20:07: #1 tags after filtering in treatment: 26486366 INFO @ Sun, 21 Jun 2020 18:20:07: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:20:07: #1 finished! INFO @ Sun, 21 Jun 2020 18:20:07: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:20:07: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:20:09: #2 number of paired peaks: 17 WARNING @ Sun, 21 Jun 2020 18:20:09: Too few paired peaks (17) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:20:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX1715676/SRX1715676.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1715676/SRX1715676.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1715676/SRX1715676.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1715676/SRX1715676.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。