Job ID = 6453963 SRX = SRX1715675 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T08:37:09 prefetch.2.10.7: 1) Downloading 'SRR3405216'... 2020-06-21T08:37:09 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T08:49:15 prefetch.2.10.7: HTTPS download succeed 2020-06-21T08:49:15 prefetch.2.10.7: 1) 'SRR3405216' was downloaded successfully Read 36344419 spots for SRR3405216/SRR3405216.sra Written 36344419 spots for SRR3405216/SRR3405216.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:11 36344419 reads; of these: 36344419 (100.00%) were unpaired; of these: 1404516 (3.86%) aligned 0 times 30029462 (82.62%) aligned exactly 1 time 4910441 (13.51%) aligned >1 times 96.14% overall alignment rate Time searching: 00:08:11 Overall time: 00:08:11 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 11269024 / 34939903 = 0.3225 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:06:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX1715675/SRX1715675.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX1715675/SRX1715675.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX1715675/SRX1715675.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX1715675/SRX1715675.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:06:27: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:06:27: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:06:33: 1000000 INFO @ Sun, 21 Jun 2020 18:06:38: 2000000 INFO @ Sun, 21 Jun 2020 18:06:44: 3000000 INFO @ Sun, 21 Jun 2020 18:06:50: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:06:56: 5000000 INFO @ Sun, 21 Jun 2020 18:06:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX1715675/SRX1715675.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX1715675/SRX1715675.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX1715675/SRX1715675.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX1715675/SRX1715675.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:06:56: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:06:56: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:07:02: 6000000 INFO @ Sun, 21 Jun 2020 18:07:04: 1000000 INFO @ Sun, 21 Jun 2020 18:07:09: 7000000 INFO @ Sun, 21 Jun 2020 18:07:12: 2000000 INFO @ Sun, 21 Jun 2020 18:07:16: 8000000 INFO @ Sun, 21 Jun 2020 18:07:20: 3000000 INFO @ Sun, 21 Jun 2020 18:07:23: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:07:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX1715675/SRX1715675.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX1715675/SRX1715675.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX1715675/SRX1715675.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX1715675/SRX1715675.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:07:26: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:07:26: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:07:28: 4000000 INFO @ Sun, 21 Jun 2020 18:07:30: 10000000 INFO @ Sun, 21 Jun 2020 18:07:35: 1000000 INFO @ Sun, 21 Jun 2020 18:07:36: 5000000 INFO @ Sun, 21 Jun 2020 18:07:38: 11000000 INFO @ Sun, 21 Jun 2020 18:07:43: 2000000 INFO @ Sun, 21 Jun 2020 18:07:44: 6000000 INFO @ Sun, 21 Jun 2020 18:07:46: 12000000 INFO @ Sun, 21 Jun 2020 18:07:51: 3000000 INFO @ Sun, 21 Jun 2020 18:07:52: 7000000 INFO @ Sun, 21 Jun 2020 18:07:53: 13000000 INFO @ Sun, 21 Jun 2020 18:07:59: 4000000 INFO @ Sun, 21 Jun 2020 18:08:00: 8000000 INFO @ Sun, 21 Jun 2020 18:08:01: 14000000 INFO @ Sun, 21 Jun 2020 18:08:07: 5000000 INFO @ Sun, 21 Jun 2020 18:08:08: 9000000 INFO @ Sun, 21 Jun 2020 18:08:09: 15000000 INFO @ Sun, 21 Jun 2020 18:08:15: 6000000 INFO @ Sun, 21 Jun 2020 18:08:16: 10000000 INFO @ Sun, 21 Jun 2020 18:08:16: 16000000 INFO @ Sun, 21 Jun 2020 18:08:23: 7000000 INFO @ Sun, 21 Jun 2020 18:08:24: 17000000 INFO @ Sun, 21 Jun 2020 18:08:24: 11000000 INFO @ Sun, 21 Jun 2020 18:08:32: 8000000 INFO @ Sun, 21 Jun 2020 18:08:32: 18000000 INFO @ Sun, 21 Jun 2020 18:08:32: 12000000 INFO @ Sun, 21 Jun 2020 18:08:40: 9000000 INFO @ Sun, 21 Jun 2020 18:08:40: 19000000 INFO @ Sun, 21 Jun 2020 18:08:41: 13000000 INFO @ Sun, 21 Jun 2020 18:08:48: 20000000 INFO @ Sun, 21 Jun 2020 18:08:48: 10000000 INFO @ Sun, 21 Jun 2020 18:08:49: 14000000 INFO @ Sun, 21 Jun 2020 18:08:55: 21000000 INFO @ Sun, 21 Jun 2020 18:08:56: 11000000 INFO @ Sun, 21 Jun 2020 18:08:57: 15000000 INFO @ Sun, 21 Jun 2020 18:09:03: 22000000 INFO @ Sun, 21 Jun 2020 18:09:05: 12000000 INFO @ Sun, 21 Jun 2020 18:09:05: 16000000 INFO @ Sun, 21 Jun 2020 18:09:10: 23000000 INFO @ Sun, 21 Jun 2020 18:09:13: 13000000 INFO @ Sun, 21 Jun 2020 18:09:14: 17000000 INFO @ Sun, 21 Jun 2020 18:09:15: #1 tag size is determined as 49 bps INFO @ Sun, 21 Jun 2020 18:09:15: #1 tag size = 49 INFO @ Sun, 21 Jun 2020 18:09:15: #1 total tags in treatment: 23670879 INFO @ Sun, 21 Jun 2020 18:09:15: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:09:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:09:15: #1 tags after filtering in treatment: 23670878 INFO @ Sun, 21 Jun 2020 18:09:15: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:09:15: #1 finished! INFO @ Sun, 21 Jun 2020 18:09:15: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:09:15: #2 looking for paired plus/minus strand peaks... BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 18:09:17: #2 number of paired peaks: 13 WARNING @ Sun, 21 Jun 2020 18:09:17: Too few paired peaks (13) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:09:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX1715675/SRX1715675.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1715675/SRX1715675.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1715675/SRX1715675.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1715675/SRX1715675.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 18:09:22: 14000000 INFO @ Sun, 21 Jun 2020 18:09:22: 18000000 INFO @ Sun, 21 Jun 2020 18:09:30: 15000000 INFO @ Sun, 21 Jun 2020 18:09:30: 19000000 INFO @ Sun, 21 Jun 2020 18:09:38: 16000000 INFO @ Sun, 21 Jun 2020 18:09:38: 20000000 INFO @ Sun, 21 Jun 2020 18:09:46: 21000000 INFO @ Sun, 21 Jun 2020 18:09:46: 17000000 INFO @ Sun, 21 Jun 2020 18:09:53: 22000000 INFO @ Sun, 21 Jun 2020 18:09:54: 18000000 INFO @ Sun, 21 Jun 2020 18:10:01: 23000000 INFO @ Sun, 21 Jun 2020 18:10:02: 19000000 INFO @ Sun, 21 Jun 2020 18:10:07: #1 tag size is determined as 49 bps INFO @ Sun, 21 Jun 2020 18:10:07: #1 tag size = 49 INFO @ Sun, 21 Jun 2020 18:10:07: #1 total tags in treatment: 23670879 INFO @ Sun, 21 Jun 2020 18:10:07: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:10:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:10:07: #1 tags after filtering in treatment: 23670878 INFO @ Sun, 21 Jun 2020 18:10:07: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:10:07: #1 finished! INFO @ Sun, 21 Jun 2020 18:10:07: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:10:07: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:10:09: #2 number of paired peaks: 13 WARNING @ Sun, 21 Jun 2020 18:10:09: Too few paired peaks (13) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:10:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX1715675/SRX1715675.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1715675/SRX1715675.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1715675/SRX1715675.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1715675/SRX1715675.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 18:10:10: 20000000 BigWig に変換しました。 INFO @ Sun, 21 Jun 2020 18:10:17: 21000000 INFO @ Sun, 21 Jun 2020 18:10:23: 22000000 INFO @ Sun, 21 Jun 2020 18:10:30: 23000000 INFO @ Sun, 21 Jun 2020 18:10:35: #1 tag size is determined as 49 bps INFO @ Sun, 21 Jun 2020 18:10:35: #1 tag size = 49 INFO @ Sun, 21 Jun 2020 18:10:35: #1 total tags in treatment: 23670879 INFO @ Sun, 21 Jun 2020 18:10:35: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:10:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:10:35: #1 tags after filtering in treatment: 23670878 INFO @ Sun, 21 Jun 2020 18:10:35: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:10:35: #1 finished! INFO @ Sun, 21 Jun 2020 18:10:35: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:10:35: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:10:37: #2 number of paired peaks: 13 WARNING @ Sun, 21 Jun 2020 18:10:37: Too few paired peaks (13) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:10:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX1715675/SRX1715675.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1715675/SRX1715675.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1715675/SRX1715675.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1715675/SRX1715675.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling