Job ID = 6453962 SRX = SRX1715674 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T09:04:08 prefetch.2.10.7: 1) Downloading 'SRR3405215'... 2020-06-21T09:04:08 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T09:11:46 prefetch.2.10.7: HTTPS download succeed 2020-06-21T09:11:46 prefetch.2.10.7: 1) 'SRR3405215' was downloaded successfully Read 36067443 spots for SRR3405215/SRR3405215.sra Written 36067443 spots for SRR3405215/SRR3405215.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:33 36067443 reads; of these: 36067443 (100.00%) were unpaired; of these: 876571 (2.43%) aligned 0 times 32168344 (89.19%) aligned exactly 1 time 3022528 (8.38%) aligned >1 times 97.57% overall alignment rate Time searching: 00:07:33 Overall time: 00:07:33 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 12916820 / 35190872 = 0.3671 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:27:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX1715674/SRX1715674.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX1715674/SRX1715674.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX1715674/SRX1715674.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX1715674/SRX1715674.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:27:53: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:27:53: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:27:58: 1000000 INFO @ Sun, 21 Jun 2020 18:28:02: 2000000 INFO @ Sun, 21 Jun 2020 18:28:07: 3000000 INFO @ Sun, 21 Jun 2020 18:28:12: 4000000 INFO @ Sun, 21 Jun 2020 18:28:16: 5000000 INFO @ Sun, 21 Jun 2020 18:28:21: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:28:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX1715674/SRX1715674.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX1715674/SRX1715674.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX1715674/SRX1715674.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX1715674/SRX1715674.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:28:23: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:28:23: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:28:26: 7000000 INFO @ Sun, 21 Jun 2020 18:28:28: 1000000 INFO @ Sun, 21 Jun 2020 18:28:31: 8000000 INFO @ Sun, 21 Jun 2020 18:28:33: 2000000 INFO @ Sun, 21 Jun 2020 18:28:36: 9000000 INFO @ Sun, 21 Jun 2020 18:28:37: 3000000 INFO @ Sun, 21 Jun 2020 18:28:41: 10000000 INFO @ Sun, 21 Jun 2020 18:28:42: 4000000 INFO @ Sun, 21 Jun 2020 18:28:45: 11000000 INFO @ Sun, 21 Jun 2020 18:28:47: 5000000 INFO @ Sun, 21 Jun 2020 18:28:50: 12000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:28:52: 6000000 INFO @ Sun, 21 Jun 2020 18:28:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX1715674/SRX1715674.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX1715674/SRX1715674.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX1715674/SRX1715674.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX1715674/SRX1715674.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:28:53: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:28:53: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:28:55: 13000000 INFO @ Sun, 21 Jun 2020 18:28:57: 7000000 INFO @ Sun, 21 Jun 2020 18:28:58: 1000000 INFO @ Sun, 21 Jun 2020 18:29:00: 14000000 INFO @ Sun, 21 Jun 2020 18:29:02: 8000000 INFO @ Sun, 21 Jun 2020 18:29:03: 2000000 INFO @ Sun, 21 Jun 2020 18:29:05: 15000000 INFO @ Sun, 21 Jun 2020 18:29:07: 9000000 INFO @ Sun, 21 Jun 2020 18:29:08: 3000000 INFO @ Sun, 21 Jun 2020 18:29:10: 16000000 INFO @ Sun, 21 Jun 2020 18:29:12: 10000000 INFO @ Sun, 21 Jun 2020 18:29:13: 4000000 INFO @ Sun, 21 Jun 2020 18:29:14: 17000000 INFO @ Sun, 21 Jun 2020 18:29:16: 11000000 INFO @ Sun, 21 Jun 2020 18:29:17: 5000000 INFO @ Sun, 21 Jun 2020 18:29:19: 18000000 INFO @ Sun, 21 Jun 2020 18:29:21: 12000000 INFO @ Sun, 21 Jun 2020 18:29:22: 6000000 INFO @ Sun, 21 Jun 2020 18:29:25: 19000000 INFO @ Sun, 21 Jun 2020 18:29:26: 13000000 INFO @ Sun, 21 Jun 2020 18:29:27: 7000000 INFO @ Sun, 21 Jun 2020 18:29:29: 20000000 INFO @ Sun, 21 Jun 2020 18:29:31: 14000000 INFO @ Sun, 21 Jun 2020 18:29:32: 8000000 INFO @ Sun, 21 Jun 2020 18:29:34: 21000000 INFO @ Sun, 21 Jun 2020 18:29:36: 15000000 INFO @ Sun, 21 Jun 2020 18:29:37: 9000000 INFO @ Sun, 21 Jun 2020 18:29:39: 22000000 INFO @ Sun, 21 Jun 2020 18:29:41: 16000000 INFO @ Sun, 21 Jun 2020 18:29:41: #1 tag size is determined as 49 bps INFO @ Sun, 21 Jun 2020 18:29:41: #1 tag size = 49 INFO @ Sun, 21 Jun 2020 18:29:41: #1 total tags in treatment: 22274052 INFO @ Sun, 21 Jun 2020 18:29:41: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:29:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:29:42: #1 tags after filtering in treatment: 22274045 INFO @ Sun, 21 Jun 2020 18:29:42: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:29:42: #1 finished! INFO @ Sun, 21 Jun 2020 18:29:42: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:29:42: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:29:42: 10000000 INFO @ Sun, 21 Jun 2020 18:29:43: #2 number of paired peaks: 14 WARNING @ Sun, 21 Jun 2020 18:29:43: Too few paired peaks (14) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:29:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX1715674/SRX1715674.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1715674/SRX1715674.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1715674/SRX1715674.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1715674/SRX1715674.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 18:29:46: 17000000 INFO @ Sun, 21 Jun 2020 18:29:47: 11000000 INFO @ Sun, 21 Jun 2020 18:29:51: 18000000 INFO @ Sun, 21 Jun 2020 18:29:52: 12000000 INFO @ Sun, 21 Jun 2020 18:29:56: 19000000 INFO @ Sun, 21 Jun 2020 18:29:57: 13000000 INFO @ Sun, 21 Jun 2020 18:30:01: 20000000 INFO @ Sun, 21 Jun 2020 18:30:02: 14000000 INFO @ Sun, 21 Jun 2020 18:30:05: 21000000 INFO @ Sun, 21 Jun 2020 18:30:06: 15000000 INFO @ Sun, 21 Jun 2020 18:30:10: 22000000 INFO @ Sun, 21 Jun 2020 18:30:11: 16000000 INFO @ Sun, 21 Jun 2020 18:30:12: #1 tag size is determined as 49 bps INFO @ Sun, 21 Jun 2020 18:30:12: #1 tag size = 49 INFO @ Sun, 21 Jun 2020 18:30:12: #1 total tags in treatment: 22274052 INFO @ Sun, 21 Jun 2020 18:30:12: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:30:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:30:13: #1 tags after filtering in treatment: 22274045 INFO @ Sun, 21 Jun 2020 18:30:13: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:30:13: #1 finished! INFO @ Sun, 21 Jun 2020 18:30:13: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:30:13: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:30:14: #2 number of paired peaks: 14 WARNING @ Sun, 21 Jun 2020 18:30:14: Too few paired peaks (14) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:30:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX1715674/SRX1715674.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1715674/SRX1715674.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1715674/SRX1715674.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1715674/SRX1715674.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 18:30:16: 17000000 INFO @ Sun, 21 Jun 2020 18:30:21: 18000000 INFO @ Sun, 21 Jun 2020 18:30:26: 19000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 18:30:31: 20000000 INFO @ Sun, 21 Jun 2020 18:30:35: 21000000 INFO @ Sun, 21 Jun 2020 18:30:40: 22000000 INFO @ Sun, 21 Jun 2020 18:30:42: #1 tag size is determined as 49 bps INFO @ Sun, 21 Jun 2020 18:30:42: #1 tag size = 49 INFO @ Sun, 21 Jun 2020 18:30:42: #1 total tags in treatment: 22274052 INFO @ Sun, 21 Jun 2020 18:30:42: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:30:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:30:42: #1 tags after filtering in treatment: 22274045 INFO @ Sun, 21 Jun 2020 18:30:42: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:30:42: #1 finished! INFO @ Sun, 21 Jun 2020 18:30:42: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:30:42: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:30:44: #2 number of paired peaks: 14 WARNING @ Sun, 21 Jun 2020 18:30:44: Too few paired peaks (14) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:30:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX1715674/SRX1715674.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1715674/SRX1715674.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1715674/SRX1715674.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1715674/SRX1715674.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。