Job ID = 6453959 SRX = SRX1715672 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T08:55:34 prefetch.2.10.7: 1) Downloading 'SRR3405213'... 2020-06-21T08:55:34 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T09:08:14 prefetch.2.10.7: HTTPS download succeed 2020-06-21T09:08:14 prefetch.2.10.7: 1) 'SRR3405213' was downloaded successfully Read 39547747 spots for SRR3405213/SRR3405213.sra Written 39547747 spots for SRR3405213/SRR3405213.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:39 39547747 reads; of these: 39547747 (100.00%) were unpaired; of these: 908798 (2.30%) aligned 0 times 33367315 (84.37%) aligned exactly 1 time 5271634 (13.33%) aligned >1 times 97.70% overall alignment rate Time searching: 00:09:39 Overall time: 00:09:39 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 14764674 / 38638949 = 0.3821 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:29:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX1715672/SRX1715672.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX1715672/SRX1715672.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX1715672/SRX1715672.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX1715672/SRX1715672.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:29:16: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:29:16: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:29:23: 1000000 INFO @ Sun, 21 Jun 2020 18:29:31: 2000000 INFO @ Sun, 21 Jun 2020 18:29:38: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:29:46: 4000000 INFO @ Sun, 21 Jun 2020 18:29:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX1715672/SRX1715672.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX1715672/SRX1715672.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX1715672/SRX1715672.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX1715672/SRX1715672.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:29:46: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:29:46: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:29:53: 1000000 INFO @ Sun, 21 Jun 2020 18:29:54: 5000000 INFO @ Sun, 21 Jun 2020 18:30:01: 2000000 INFO @ Sun, 21 Jun 2020 18:30:02: 6000000 INFO @ Sun, 21 Jun 2020 18:30:08: 3000000 INFO @ Sun, 21 Jun 2020 18:30:11: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:30:15: 4000000 INFO @ Sun, 21 Jun 2020 18:30:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX1715672/SRX1715672.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX1715672/SRX1715672.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX1715672/SRX1715672.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX1715672/SRX1715672.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:30:16: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:30:16: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:30:19: 8000000 INFO @ Sun, 21 Jun 2020 18:30:23: 5000000 INFO @ Sun, 21 Jun 2020 18:30:23: 1000000 INFO @ Sun, 21 Jun 2020 18:30:27: 9000000 INFO @ Sun, 21 Jun 2020 18:30:30: 6000000 INFO @ Sun, 21 Jun 2020 18:30:31: 2000000 INFO @ Sun, 21 Jun 2020 18:30:36: 10000000 INFO @ Sun, 21 Jun 2020 18:30:38: 7000000 INFO @ Sun, 21 Jun 2020 18:30:38: 3000000 INFO @ Sun, 21 Jun 2020 18:30:44: 11000000 INFO @ Sun, 21 Jun 2020 18:30:45: 8000000 INFO @ Sun, 21 Jun 2020 18:30:46: 4000000 INFO @ Sun, 21 Jun 2020 18:30:52: 9000000 INFO @ Sun, 21 Jun 2020 18:30:53: 12000000 INFO @ Sun, 21 Jun 2020 18:30:53: 5000000 INFO @ Sun, 21 Jun 2020 18:31:00: 10000000 INFO @ Sun, 21 Jun 2020 18:31:01: 6000000 INFO @ Sun, 21 Jun 2020 18:31:01: 13000000 INFO @ Sun, 21 Jun 2020 18:31:08: 11000000 INFO @ Sun, 21 Jun 2020 18:31:08: 7000000 INFO @ Sun, 21 Jun 2020 18:31:10: 14000000 INFO @ Sun, 21 Jun 2020 18:31:15: 12000000 INFO @ Sun, 21 Jun 2020 18:31:16: 8000000 INFO @ Sun, 21 Jun 2020 18:31:19: 15000000 INFO @ Sun, 21 Jun 2020 18:31:23: 13000000 INFO @ Sun, 21 Jun 2020 18:31:24: 9000000 INFO @ Sun, 21 Jun 2020 18:31:29: 16000000 INFO @ Sun, 21 Jun 2020 18:31:31: 14000000 INFO @ Sun, 21 Jun 2020 18:31:32: 10000000 INFO @ Sun, 21 Jun 2020 18:31:38: 17000000 INFO @ Sun, 21 Jun 2020 18:31:39: 15000000 INFO @ Sun, 21 Jun 2020 18:31:39: 11000000 INFO @ Sun, 21 Jun 2020 18:31:46: 16000000 INFO @ Sun, 21 Jun 2020 18:31:47: 18000000 INFO @ Sun, 21 Jun 2020 18:31:47: 12000000 INFO @ Sun, 21 Jun 2020 18:31:54: 17000000 INFO @ Sun, 21 Jun 2020 18:31:55: 13000000 INFO @ Sun, 21 Jun 2020 18:31:55: 19000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 18:32:02: 18000000 INFO @ Sun, 21 Jun 2020 18:32:03: 14000000 INFO @ Sun, 21 Jun 2020 18:32:04: 20000000 INFO @ Sun, 21 Jun 2020 18:32:10: 19000000 INFO @ Sun, 21 Jun 2020 18:32:10: 15000000 INFO @ Sun, 21 Jun 2020 18:32:14: 21000000 INFO @ Sun, 21 Jun 2020 18:32:18: 20000000 INFO @ Sun, 21 Jun 2020 18:32:18: 16000000 INFO @ Sun, 21 Jun 2020 18:32:22: 22000000 INFO @ Sun, 21 Jun 2020 18:32:25: 21000000 INFO @ Sun, 21 Jun 2020 18:32:26: 17000000 INFO @ Sun, 21 Jun 2020 18:32:31: 23000000 INFO @ Sun, 21 Jun 2020 18:32:33: 22000000 INFO @ Sun, 21 Jun 2020 18:32:33: 18000000 INFO @ Sun, 21 Jun 2020 18:32:39: #1 tag size is determined as 49 bps INFO @ Sun, 21 Jun 2020 18:32:39: #1 tag size = 49 INFO @ Sun, 21 Jun 2020 18:32:39: #1 total tags in treatment: 23874275 INFO @ Sun, 21 Jun 2020 18:32:39: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:32:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:32:40: #1 tags after filtering in treatment: 23874271 INFO @ Sun, 21 Jun 2020 18:32:40: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:32:40: #1 finished! INFO @ Sun, 21 Jun 2020 18:32:40: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:32:40: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:32:41: 23000000 INFO @ Sun, 21 Jun 2020 18:32:41: 19000000 INFO @ Sun, 21 Jun 2020 18:32:42: #2 number of paired peaks: 5 WARNING @ Sun, 21 Jun 2020 18:32:42: Too few paired peaks (5) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:32:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX1715672/SRX1715672.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1715672/SRX1715672.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1715672/SRX1715672.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1715672/SRX1715672.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 18:32:48: #1 tag size is determined as 49 bps INFO @ Sun, 21 Jun 2020 18:32:48: #1 tag size = 49 INFO @ Sun, 21 Jun 2020 18:32:48: #1 total tags in treatment: 23874275 INFO @ Sun, 21 Jun 2020 18:32:48: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:32:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:32:48: #1 tags after filtering in treatment: 23874271 INFO @ Sun, 21 Jun 2020 18:32:48: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:32:48: #1 finished! INFO @ Sun, 21 Jun 2020 18:32:48: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:32:48: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:32:49: 20000000 INFO @ Sun, 21 Jun 2020 18:32:50: #2 number of paired peaks: 5 WARNING @ Sun, 21 Jun 2020 18:32:50: Too few paired peaks (5) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:32:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX1715672/SRX1715672.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1715672/SRX1715672.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1715672/SRX1715672.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1715672/SRX1715672.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。 INFO @ Sun, 21 Jun 2020 18:32:55: 21000000 INFO @ Sun, 21 Jun 2020 18:33:01: 22000000 INFO @ Sun, 21 Jun 2020 18:33:07: 23000000 INFO @ Sun, 21 Jun 2020 18:33:13: #1 tag size is determined as 49 bps INFO @ Sun, 21 Jun 2020 18:33:13: #1 tag size = 49 INFO @ Sun, 21 Jun 2020 18:33:13: #1 total tags in treatment: 23874275 INFO @ Sun, 21 Jun 2020 18:33:13: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:33:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:33:13: #1 tags after filtering in treatment: 23874271 INFO @ Sun, 21 Jun 2020 18:33:13: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:33:13: #1 finished! INFO @ Sun, 21 Jun 2020 18:33:13: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:33:13: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:33:15: #2 number of paired peaks: 5 WARNING @ Sun, 21 Jun 2020 18:33:15: Too few paired peaks (5) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:33:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX1715672/SRX1715672.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 3 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1715672/SRX1715672.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1715672/SRX1715672.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1715672/SRX1715672.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling