Job ID = 6453922 SRX = SRX1600286 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T08:43:33 prefetch.2.10.7: 1) Downloading 'SRR3187709'... 2020-06-21T08:43:33 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T08:50:54 prefetch.2.10.7: HTTPS download succeed 2020-06-21T08:50:54 prefetch.2.10.7: 1) 'SRR3187709' was downloaded successfully Read 36549214 spots for SRR3187709/SRR3187709.sra Written 36549214 spots for SRR3187709/SRR3187709.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:39 36549214 reads; of these: 36549214 (100.00%) were unpaired; of these: 5415512 (14.82%) aligned 0 times 24900931 (68.13%) aligned exactly 1 time 6232771 (17.05%) aligned >1 times 85.18% overall alignment rate Time searching: 00:11:39 Overall time: 00:11:39 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 8155293 / 31133702 = 0.2619 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:14:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX1600286/SRX1600286.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX1600286/SRX1600286.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX1600286/SRX1600286.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX1600286/SRX1600286.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:14:13: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:14:13: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:14:21: 1000000 INFO @ Sun, 21 Jun 2020 18:14:28: 2000000 INFO @ Sun, 21 Jun 2020 18:14:35: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:14:42: 4000000 INFO @ Sun, 21 Jun 2020 18:14:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX1600286/SRX1600286.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX1600286/SRX1600286.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX1600286/SRX1600286.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX1600286/SRX1600286.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:14:43: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:14:43: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:14:50: 5000000 INFO @ Sun, 21 Jun 2020 18:14:51: 1000000 INFO @ Sun, 21 Jun 2020 18:14:58: 2000000 INFO @ Sun, 21 Jun 2020 18:14:58: 6000000 INFO @ Sun, 21 Jun 2020 18:15:05: 3000000 INFO @ Sun, 21 Jun 2020 18:15:06: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:15:12: 4000000 INFO @ Sun, 21 Jun 2020 18:15:13: 8000000 INFO @ Sun, 21 Jun 2020 18:15:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX1600286/SRX1600286.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX1600286/SRX1600286.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX1600286/SRX1600286.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX1600286/SRX1600286.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:15:14: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:15:14: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:15:19: 5000000 INFO @ Sun, 21 Jun 2020 18:15:21: 9000000 INFO @ Sun, 21 Jun 2020 18:15:21: 1000000 INFO @ Sun, 21 Jun 2020 18:15:27: 6000000 INFO @ Sun, 21 Jun 2020 18:15:29: 10000000 INFO @ Sun, 21 Jun 2020 18:15:29: 2000000 INFO @ Sun, 21 Jun 2020 18:15:34: 7000000 INFO @ Sun, 21 Jun 2020 18:15:37: 11000000 INFO @ Sun, 21 Jun 2020 18:15:37: 3000000 INFO @ Sun, 21 Jun 2020 18:15:41: 8000000 INFO @ Sun, 21 Jun 2020 18:15:45: 12000000 INFO @ Sun, 21 Jun 2020 18:15:45: 4000000 INFO @ Sun, 21 Jun 2020 18:15:48: 9000000 INFO @ Sun, 21 Jun 2020 18:15:53: 13000000 INFO @ Sun, 21 Jun 2020 18:15:53: 5000000 INFO @ Sun, 21 Jun 2020 18:15:55: 10000000 INFO @ Sun, 21 Jun 2020 18:16:01: 14000000 INFO @ Sun, 21 Jun 2020 18:16:01: 6000000 INFO @ Sun, 21 Jun 2020 18:16:03: 11000000 INFO @ Sun, 21 Jun 2020 18:16:09: 15000000 INFO @ Sun, 21 Jun 2020 18:16:09: 7000000 INFO @ Sun, 21 Jun 2020 18:16:10: 12000000 INFO @ Sun, 21 Jun 2020 18:16:16: 8000000 INFO @ Sun, 21 Jun 2020 18:16:17: 16000000 INFO @ Sun, 21 Jun 2020 18:16:17: 13000000 INFO @ Sun, 21 Jun 2020 18:16:24: 14000000 INFO @ Sun, 21 Jun 2020 18:16:24: 9000000 INFO @ Sun, 21 Jun 2020 18:16:25: 17000000 INFO @ Sun, 21 Jun 2020 18:16:31: 15000000 INFO @ Sun, 21 Jun 2020 18:16:32: 10000000 INFO @ Sun, 21 Jun 2020 18:16:32: 18000000 INFO @ Sun, 21 Jun 2020 18:16:38: 16000000 INFO @ Sun, 21 Jun 2020 18:16:40: 11000000 INFO @ Sun, 21 Jun 2020 18:16:41: 19000000 INFO @ Sun, 21 Jun 2020 18:16:46: 17000000 INFO @ Sun, 21 Jun 2020 18:16:48: 12000000 INFO @ Sun, 21 Jun 2020 18:16:49: 20000000 INFO @ Sun, 21 Jun 2020 18:16:53: 18000000 INFO @ Sun, 21 Jun 2020 18:16:55: 13000000 INFO @ Sun, 21 Jun 2020 18:16:57: 21000000 INFO @ Sun, 21 Jun 2020 18:17:00: 19000000 INFO @ Sun, 21 Jun 2020 18:17:03: 14000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 18:17:05: 22000000 INFO @ Sun, 21 Jun 2020 18:17:08: 20000000 INFO @ Sun, 21 Jun 2020 18:17:11: 15000000 INFO @ Sun, 21 Jun 2020 18:17:13: #1 tag size is determined as 76 bps INFO @ Sun, 21 Jun 2020 18:17:13: #1 tag size = 76 INFO @ Sun, 21 Jun 2020 18:17:13: #1 total tags in treatment: 22978409 INFO @ Sun, 21 Jun 2020 18:17:13: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:17:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:17:14: #1 tags after filtering in treatment: 22978405 INFO @ Sun, 21 Jun 2020 18:17:14: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:17:14: #1 finished! INFO @ Sun, 21 Jun 2020 18:17:14: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:17:14: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:17:15: 21000000 INFO @ Sun, 21 Jun 2020 18:17:15: #2 number of paired peaks: 22 WARNING @ Sun, 21 Jun 2020 18:17:15: Too few paired peaks (22) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:17:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX1600286/SRX1600286.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1600286/SRX1600286.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1600286/SRX1600286.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1600286/SRX1600286.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 18:17:18: 16000000 INFO @ Sun, 21 Jun 2020 18:17:22: 22000000 INFO @ Sun, 21 Jun 2020 18:17:26: 17000000 INFO @ Sun, 21 Jun 2020 18:17:29: #1 tag size is determined as 76 bps INFO @ Sun, 21 Jun 2020 18:17:29: #1 tag size = 76 INFO @ Sun, 21 Jun 2020 18:17:29: #1 total tags in treatment: 22978409 INFO @ Sun, 21 Jun 2020 18:17:29: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:17:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:17:30: #1 tags after filtering in treatment: 22978405 INFO @ Sun, 21 Jun 2020 18:17:30: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:17:30: #1 finished! INFO @ Sun, 21 Jun 2020 18:17:30: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:17:30: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:17:31: #2 number of paired peaks: 22 WARNING @ Sun, 21 Jun 2020 18:17:31: Too few paired peaks (22) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:17:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX1600286/SRX1600286.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1600286/SRX1600286.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1600286/SRX1600286.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1600286/SRX1600286.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 18:17:33: 18000000 INFO @ Sun, 21 Jun 2020 18:17:41: 19000000 INFO @ Sun, 21 Jun 2020 18:17:48: 20000000 INFO @ Sun, 21 Jun 2020 18:17:56: 21000000 BigWig に変換しました。 INFO @ Sun, 21 Jun 2020 18:18:03: 22000000 INFO @ Sun, 21 Jun 2020 18:18:10: #1 tag size is determined as 76 bps INFO @ Sun, 21 Jun 2020 18:18:10: #1 tag size = 76 INFO @ Sun, 21 Jun 2020 18:18:10: #1 total tags in treatment: 22978409 INFO @ Sun, 21 Jun 2020 18:18:10: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:18:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:18:11: #1 tags after filtering in treatment: 22978405 INFO @ Sun, 21 Jun 2020 18:18:11: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:18:11: #1 finished! INFO @ Sun, 21 Jun 2020 18:18:11: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:18:11: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:18:12: #2 number of paired peaks: 22 WARNING @ Sun, 21 Jun 2020 18:18:12: Too few paired peaks (22) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:18:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX1600286/SRX1600286.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1600286/SRX1600286.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1600286/SRX1600286.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX1600286/SRX1600286.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling