Job ID = 6453905 SRX = SRX159164 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T08:51:19 prefetch.2.10.7: 1) Downloading 'SRR520445'... 2020-06-21T08:51:19 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T09:02:14 prefetch.2.10.7: HTTPS download succeed 2020-06-21T09:02:14 prefetch.2.10.7: 1) 'SRR520445' was downloaded successfully Read 79102347 spots for SRR520445/SRR520445.sra Written 79102347 spots for SRR520445/SRR520445.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:22:33 79102347 reads; of these: 79102347 (100.00%) were unpaired; of these: 6648837 (8.41%) aligned 0 times 53640274 (67.81%) aligned exactly 1 time 18813236 (23.78%) aligned >1 times 91.59% overall alignment rate Time searching: 00:22:33 Overall time: 00:22:33 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 32 files... [bam_rmdupse_core] 27961810 / 72453510 = 0.3859 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:46:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX159164/SRX159164.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX159164/SRX159164.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX159164/SRX159164.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX159164/SRX159164.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:46:17: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:46:17: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:46:24: 1000000 INFO @ Sun, 21 Jun 2020 18:46:31: 2000000 INFO @ Sun, 21 Jun 2020 18:46:38: 3000000 INFO @ Sun, 21 Jun 2020 18:46:45: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:46:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX159164/SRX159164.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX159164/SRX159164.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX159164/SRX159164.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX159164/SRX159164.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:46:48: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:46:48: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:46:52: 5000000 INFO @ Sun, 21 Jun 2020 18:46:55: 1000000 INFO @ Sun, 21 Jun 2020 18:47:00: 6000000 INFO @ Sun, 21 Jun 2020 18:47:02: 2000000 INFO @ Sun, 21 Jun 2020 18:47:07: 7000000 INFO @ Sun, 21 Jun 2020 18:47:09: 3000000 INFO @ Sun, 21 Jun 2020 18:47:15: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:47:16: 4000000 INFO @ Sun, 21 Jun 2020 18:47:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX159164/SRX159164.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX159164/SRX159164.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX159164/SRX159164.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX159164/SRX159164.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:47:18: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:47:18: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:47:22: 9000000 INFO @ Sun, 21 Jun 2020 18:47:23: 5000000 INFO @ Sun, 21 Jun 2020 18:47:25: 1000000 INFO @ Sun, 21 Jun 2020 18:47:30: 10000000 INFO @ Sun, 21 Jun 2020 18:47:31: 6000000 INFO @ Sun, 21 Jun 2020 18:47:32: 2000000 INFO @ Sun, 21 Jun 2020 18:47:38: 11000000 INFO @ Sun, 21 Jun 2020 18:47:38: 7000000 INFO @ Sun, 21 Jun 2020 18:47:40: 3000000 INFO @ Sun, 21 Jun 2020 18:47:45: 12000000 INFO @ Sun, 21 Jun 2020 18:47:45: 8000000 INFO @ Sun, 21 Jun 2020 18:47:47: 4000000 INFO @ Sun, 21 Jun 2020 18:47:53: 9000000 INFO @ Sun, 21 Jun 2020 18:47:53: 13000000 INFO @ Sun, 21 Jun 2020 18:47:54: 5000000 INFO @ Sun, 21 Jun 2020 18:48:00: 10000000 INFO @ Sun, 21 Jun 2020 18:48:00: 14000000 INFO @ Sun, 21 Jun 2020 18:48:02: 6000000 INFO @ Sun, 21 Jun 2020 18:48:07: 11000000 INFO @ Sun, 21 Jun 2020 18:48:08: 15000000 INFO @ Sun, 21 Jun 2020 18:48:09: 7000000 INFO @ Sun, 21 Jun 2020 18:48:15: 12000000 INFO @ Sun, 21 Jun 2020 18:48:16: 16000000 INFO @ Sun, 21 Jun 2020 18:48:16: 8000000 INFO @ Sun, 21 Jun 2020 18:48:22: 13000000 INFO @ Sun, 21 Jun 2020 18:48:23: 17000000 INFO @ Sun, 21 Jun 2020 18:48:24: 9000000 INFO @ Sun, 21 Jun 2020 18:48:29: 14000000 INFO @ Sun, 21 Jun 2020 18:48:31: 18000000 INFO @ Sun, 21 Jun 2020 18:48:31: 10000000 INFO @ Sun, 21 Jun 2020 18:48:37: 15000000 INFO @ Sun, 21 Jun 2020 18:48:39: 11000000 INFO @ Sun, 21 Jun 2020 18:48:39: 19000000 INFO @ Sun, 21 Jun 2020 18:48:44: 16000000 INFO @ Sun, 21 Jun 2020 18:48:46: 12000000 INFO @ Sun, 21 Jun 2020 18:48:47: 20000000 INFO @ Sun, 21 Jun 2020 18:48:52: 17000000 INFO @ Sun, 21 Jun 2020 18:48:53: 13000000 INFO @ Sun, 21 Jun 2020 18:48:54: 21000000 INFO @ Sun, 21 Jun 2020 18:48:59: 18000000 INFO @ Sun, 21 Jun 2020 18:49:01: 14000000 INFO @ Sun, 21 Jun 2020 18:49:02: 22000000 INFO @ Sun, 21 Jun 2020 18:49:07: 19000000 INFO @ Sun, 21 Jun 2020 18:49:08: 15000000 INFO @ Sun, 21 Jun 2020 18:49:10: 23000000 INFO @ Sun, 21 Jun 2020 18:49:15: 20000000 INFO @ Sun, 21 Jun 2020 18:49:16: 16000000 INFO @ Sun, 21 Jun 2020 18:49:18: 24000000 INFO @ Sun, 21 Jun 2020 18:49:23: 21000000 INFO @ Sun, 21 Jun 2020 18:49:24: 17000000 INFO @ Sun, 21 Jun 2020 18:49:25: 25000000 INFO @ Sun, 21 Jun 2020 18:49:31: 22000000 INFO @ Sun, 21 Jun 2020 18:49:31: 18000000 INFO @ Sun, 21 Jun 2020 18:49:33: 26000000 INFO @ Sun, 21 Jun 2020 18:49:39: 23000000 INFO @ Sun, 21 Jun 2020 18:49:39: 19000000 INFO @ Sun, 21 Jun 2020 18:49:41: 27000000 INFO @ Sun, 21 Jun 2020 18:49:46: 24000000 INFO @ Sun, 21 Jun 2020 18:49:47: 20000000 INFO @ Sun, 21 Jun 2020 18:49:48: 28000000 INFO @ Sun, 21 Jun 2020 18:49:54: 25000000 INFO @ Sun, 21 Jun 2020 18:49:55: 21000000 INFO @ Sun, 21 Jun 2020 18:49:56: 29000000 INFO @ Sun, 21 Jun 2020 18:50:02: 26000000 INFO @ Sun, 21 Jun 2020 18:50:03: 22000000 INFO @ Sun, 21 Jun 2020 18:50:04: 30000000 INFO @ Sun, 21 Jun 2020 18:50:10: 27000000 INFO @ Sun, 21 Jun 2020 18:50:11: 23000000 INFO @ Sun, 21 Jun 2020 18:50:12: 31000000 INFO @ Sun, 21 Jun 2020 18:50:18: 28000000 INFO @ Sun, 21 Jun 2020 18:50:18: 24000000 INFO @ Sun, 21 Jun 2020 18:50:19: 32000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 18:50:25: 29000000 INFO @ Sun, 21 Jun 2020 18:50:26: 25000000 INFO @ Sun, 21 Jun 2020 18:50:27: 33000000 INFO @ Sun, 21 Jun 2020 18:50:33: 30000000 INFO @ Sun, 21 Jun 2020 18:50:33: 26000000 INFO @ Sun, 21 Jun 2020 18:50:35: 34000000 INFO @ Sun, 21 Jun 2020 18:50:41: 27000000 INFO @ Sun, 21 Jun 2020 18:50:41: 31000000 INFO @ Sun, 21 Jun 2020 18:50:42: 35000000 INFO @ Sun, 21 Jun 2020 18:50:48: 28000000 INFO @ Sun, 21 Jun 2020 18:50:49: 32000000 INFO @ Sun, 21 Jun 2020 18:50:50: 36000000 INFO @ Sun, 21 Jun 2020 18:50:56: 29000000 INFO @ Sun, 21 Jun 2020 18:50:57: 33000000 INFO @ Sun, 21 Jun 2020 18:50:58: 37000000 INFO @ Sun, 21 Jun 2020 18:51:03: 30000000 INFO @ Sun, 21 Jun 2020 18:51:04: 34000000 INFO @ Sun, 21 Jun 2020 18:51:05: 38000000 INFO @ Sun, 21 Jun 2020 18:51:11: 31000000 INFO @ Sun, 21 Jun 2020 18:51:12: 35000000 INFO @ Sun, 21 Jun 2020 18:51:13: 39000000 INFO @ Sun, 21 Jun 2020 18:51:19: 32000000 INFO @ Sun, 21 Jun 2020 18:51:20: 36000000 INFO @ Sun, 21 Jun 2020 18:51:21: 40000000 INFO @ Sun, 21 Jun 2020 18:51:26: 33000000 INFO @ Sun, 21 Jun 2020 18:51:27: 37000000 INFO @ Sun, 21 Jun 2020 18:51:29: 41000000 INFO @ Sun, 21 Jun 2020 18:51:33: 34000000 INFO @ Sun, 21 Jun 2020 18:51:34: 38000000 INFO @ Sun, 21 Jun 2020 18:51:36: 42000000 INFO @ Sun, 21 Jun 2020 18:51:41: 35000000 INFO @ Sun, 21 Jun 2020 18:51:42: 39000000 INFO @ Sun, 21 Jun 2020 18:51:44: 43000000 BigWig に変換しました。 INFO @ Sun, 21 Jun 2020 18:51:48: 36000000 INFO @ Sun, 21 Jun 2020 18:51:49: 40000000 INFO @ Sun, 21 Jun 2020 18:51:51: 44000000 INFO @ Sun, 21 Jun 2020 18:51:55: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 18:51:55: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 18:51:55: #1 total tags in treatment: 44491700 INFO @ Sun, 21 Jun 2020 18:51:55: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:51:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:51:56: 37000000 INFO @ Sun, 21 Jun 2020 18:51:56: #1 tags after filtering in treatment: 44491663 INFO @ Sun, 21 Jun 2020 18:51:56: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:51:56: #1 finished! INFO @ Sun, 21 Jun 2020 18:51:56: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:51:56: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:51:57: 41000000 INFO @ Sun, 21 Jun 2020 18:51:59: #2 number of paired peaks: 41 WARNING @ Sun, 21 Jun 2020 18:51:59: Too few paired peaks (41) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:51:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX159164/SRX159164.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX159164/SRX159164.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX159164/SRX159164.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX159164/SRX159164.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 18:52:03: 38000000 INFO @ Sun, 21 Jun 2020 18:52:04: 42000000 INFO @ Sun, 21 Jun 2020 18:52:10: 39000000 INFO @ Sun, 21 Jun 2020 18:52:11: 43000000 INFO @ Sun, 21 Jun 2020 18:52:17: 40000000 INFO @ Sun, 21 Jun 2020 18:52:18: 44000000 INFO @ Sun, 21 Jun 2020 18:52:22: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 18:52:22: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 18:52:22: #1 total tags in treatment: 44491700 INFO @ Sun, 21 Jun 2020 18:52:22: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:52:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:52:23: #1 tags after filtering in treatment: 44491663 INFO @ Sun, 21 Jun 2020 18:52:23: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:52:23: #1 finished! INFO @ Sun, 21 Jun 2020 18:52:23: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:52:23: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:52:24: 41000000 INFO @ Sun, 21 Jun 2020 18:52:26: #2 number of paired peaks: 41 WARNING @ Sun, 21 Jun 2020 18:52:26: Too few paired peaks (41) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:52:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX159164/SRX159164.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX159164/SRX159164.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX159164/SRX159164.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX159164/SRX159164.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 18:52:31: 42000000 INFO @ Sun, 21 Jun 2020 18:52:38: 43000000 INFO @ Sun, 21 Jun 2020 18:52:45: 44000000 INFO @ Sun, 21 Jun 2020 18:52:48: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 18:52:48: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 18:52:48: #1 total tags in treatment: 44491700 INFO @ Sun, 21 Jun 2020 18:52:48: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:52:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:52:49: #1 tags after filtering in treatment: 44491663 INFO @ Sun, 21 Jun 2020 18:52:49: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:52:49: #1 finished! INFO @ Sun, 21 Jun 2020 18:52:49: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:52:49: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:52:52: #2 number of paired peaks: 41 WARNING @ Sun, 21 Jun 2020 18:52:52: Too few paired peaks (41) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:52:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX159164/SRX159164.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX159164/SRX159164.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX159164/SRX159164.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX159164/SRX159164.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling