Job ID = 6453848 SRX = SRX152103 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T08:40:17 prefetch.2.10.7: 1) Downloading 'SRR504959'... 2020-06-21T08:40:17 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T08:46:49 prefetch.2.10.7: HTTPS download succeed 2020-06-21T08:46:49 prefetch.2.10.7: 1) 'SRR504959' was downloaded successfully Read 37203045 spots for SRR504959/SRR504959.sra Written 37203045 spots for SRR504959/SRR504959.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:39 37203045 reads; of these: 37203045 (100.00%) were unpaired; of these: 1742482 (4.68%) aligned 0 times 25150174 (67.60%) aligned exactly 1 time 10310389 (27.71%) aligned >1 times 95.32% overall alignment rate Time searching: 00:11:39 Overall time: 00:11:39 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 10382281 / 35460563 = 0.2928 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:09:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX152103/SRX152103.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX152103/SRX152103.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX152103/SRX152103.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX152103/SRX152103.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:09:45: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:09:45: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:09:51: 1000000 INFO @ Sun, 21 Jun 2020 18:09:58: 2000000 INFO @ Sun, 21 Jun 2020 18:10:04: 3000000 INFO @ Sun, 21 Jun 2020 18:10:10: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:10:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX152103/SRX152103.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX152103/SRX152103.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX152103/SRX152103.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX152103/SRX152103.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:10:15: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:10:15: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:10:16: 5000000 INFO @ Sun, 21 Jun 2020 18:10:23: 1000000 INFO @ Sun, 21 Jun 2020 18:10:23: 6000000 INFO @ Sun, 21 Jun 2020 18:10:31: 2000000 INFO @ Sun, 21 Jun 2020 18:10:31: 7000000 INFO @ Sun, 21 Jun 2020 18:10:38: 8000000 INFO @ Sun, 21 Jun 2020 18:10:38: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:10:45: 9000000 INFO @ Sun, 21 Jun 2020 18:10:45: 4000000 INFO @ Sun, 21 Jun 2020 18:10:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX152103/SRX152103.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX152103/SRX152103.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX152103/SRX152103.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX152103/SRX152103.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:10:45: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:10:45: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:10:52: 10000000 INFO @ Sun, 21 Jun 2020 18:10:53: 1000000 INFO @ Sun, 21 Jun 2020 18:10:53: 5000000 INFO @ Sun, 21 Jun 2020 18:10:59: 11000000 INFO @ Sun, 21 Jun 2020 18:11:00: 2000000 INFO @ Sun, 21 Jun 2020 18:11:00: 6000000 INFO @ Sun, 21 Jun 2020 18:11:06: 12000000 INFO @ Sun, 21 Jun 2020 18:11:07: 3000000 INFO @ Sun, 21 Jun 2020 18:11:08: 7000000 INFO @ Sun, 21 Jun 2020 18:11:13: 13000000 INFO @ Sun, 21 Jun 2020 18:11:15: 8000000 INFO @ Sun, 21 Jun 2020 18:11:15: 4000000 INFO @ Sun, 21 Jun 2020 18:11:20: 14000000 INFO @ Sun, 21 Jun 2020 18:11:22: 9000000 INFO @ Sun, 21 Jun 2020 18:11:22: 5000000 INFO @ Sun, 21 Jun 2020 18:11:28: 15000000 INFO @ Sun, 21 Jun 2020 18:11:29: 10000000 INFO @ Sun, 21 Jun 2020 18:11:30: 6000000 INFO @ Sun, 21 Jun 2020 18:11:34: 16000000 INFO @ Sun, 21 Jun 2020 18:11:36: 11000000 INFO @ Sun, 21 Jun 2020 18:11:37: 7000000 INFO @ Sun, 21 Jun 2020 18:11:41: 17000000 INFO @ Sun, 21 Jun 2020 18:11:43: 12000000 INFO @ Sun, 21 Jun 2020 18:11:44: 8000000 INFO @ Sun, 21 Jun 2020 18:11:48: 18000000 INFO @ Sun, 21 Jun 2020 18:11:50: 13000000 INFO @ Sun, 21 Jun 2020 18:11:51: 9000000 INFO @ Sun, 21 Jun 2020 18:11:55: 19000000 INFO @ Sun, 21 Jun 2020 18:11:56: 14000000 INFO @ Sun, 21 Jun 2020 18:11:58: 10000000 INFO @ Sun, 21 Jun 2020 18:12:02: 20000000 INFO @ Sun, 21 Jun 2020 18:12:03: 15000000 INFO @ Sun, 21 Jun 2020 18:12:05: 11000000 INFO @ Sun, 21 Jun 2020 18:12:09: 21000000 INFO @ Sun, 21 Jun 2020 18:12:10: 16000000 INFO @ Sun, 21 Jun 2020 18:12:12: 12000000 INFO @ Sun, 21 Jun 2020 18:12:16: 22000000 INFO @ Sun, 21 Jun 2020 18:12:16: 17000000 INFO @ Sun, 21 Jun 2020 18:12:19: 13000000 INFO @ Sun, 21 Jun 2020 18:12:23: 23000000 INFO @ Sun, 21 Jun 2020 18:12:23: 18000000 INFO @ Sun, 21 Jun 2020 18:12:26: 14000000 INFO @ Sun, 21 Jun 2020 18:12:29: 24000000 INFO @ Sun, 21 Jun 2020 18:12:30: 19000000 INFO @ Sun, 21 Jun 2020 18:12:32: 15000000 INFO @ Sun, 21 Jun 2020 18:12:36: 25000000 INFO @ Sun, 21 Jun 2020 18:12:36: 20000000 INFO @ Sun, 21 Jun 2020 18:12:37: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 18:12:37: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 18:12:37: #1 total tags in treatment: 25078282 INFO @ Sun, 21 Jun 2020 18:12:37: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:12:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:12:38: #1 tags after filtering in treatment: 25078282 INFO @ Sun, 21 Jun 2020 18:12:38: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:12:38: #1 finished! INFO @ Sun, 21 Jun 2020 18:12:38: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:12:38: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:12:39: 16000000 INFO @ Sun, 21 Jun 2020 18:12:39: #2 number of paired peaks: 89 WARNING @ Sun, 21 Jun 2020 18:12:39: Too few paired peaks (89) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:12:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX152103/SRX152103.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX152103/SRX152103.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX152103/SRX152103.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX152103/SRX152103.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 18:12:43: 21000000 INFO @ Sun, 21 Jun 2020 18:12:45: 17000000 INFO @ Sun, 21 Jun 2020 18:12:50: 22000000 INFO @ Sun, 21 Jun 2020 18:12:52: 18000000 INFO @ Sun, 21 Jun 2020 18:12:56: 23000000 INFO @ Sun, 21 Jun 2020 18:12:58: 19000000 INFO @ Sun, 21 Jun 2020 18:13:03: 24000000 INFO @ Sun, 21 Jun 2020 18:13:05: 20000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 18:13:09: 25000000 INFO @ Sun, 21 Jun 2020 18:13:10: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 18:13:10: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 18:13:10: #1 total tags in treatment: 25078282 INFO @ Sun, 21 Jun 2020 18:13:10: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:13:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:13:11: #1 tags after filtering in treatment: 25078282 INFO @ Sun, 21 Jun 2020 18:13:11: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:13:11: #1 finished! INFO @ Sun, 21 Jun 2020 18:13:11: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:13:11: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:13:12: 21000000 INFO @ Sun, 21 Jun 2020 18:13:12: #2 number of paired peaks: 89 WARNING @ Sun, 21 Jun 2020 18:13:12: Too few paired peaks (89) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:13:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX152103/SRX152103.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX152103/SRX152103.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX152103/SRX152103.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX152103/SRX152103.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 18:13:18: 22000000 INFO @ Sun, 21 Jun 2020 18:13:24: 23000000 INFO @ Sun, 21 Jun 2020 18:13:30: 24000000 INFO @ Sun, 21 Jun 2020 18:13:36: 25000000 INFO @ Sun, 21 Jun 2020 18:13:36: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 18:13:36: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 18:13:36: #1 total tags in treatment: 25078282 INFO @ Sun, 21 Jun 2020 18:13:36: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:13:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:13:37: #1 tags after filtering in treatment: 25078282 INFO @ Sun, 21 Jun 2020 18:13:37: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:13:37: #1 finished! INFO @ Sun, 21 Jun 2020 18:13:37: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:13:37: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:13:39: #2 number of paired peaks: 89 WARNING @ Sun, 21 Jun 2020 18:13:39: Too few paired peaks (89) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:13:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX152103/SRX152103.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX152103/SRX152103.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX152103/SRX152103.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX152103/SRX152103.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。