Job ID = 6453846 SRX = SRX152101 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T08:46:17 prefetch.2.10.7: 1) Downloading 'SRR504957'... 2020-06-21T08:46:17 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T08:49:50 prefetch.2.10.7: HTTPS download succeed 2020-06-21T08:49:50 prefetch.2.10.7: 1) 'SRR504957' was downloaded successfully Read 29985092 spots for SRR504957/SRR504957.sra Written 29985092 spots for SRR504957/SRR504957.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:04 29985092 reads; of these: 29985092 (100.00%) were unpaired; of these: 560390 (1.87%) aligned 0 times 21977378 (73.29%) aligned exactly 1 time 7447324 (24.84%) aligned >1 times 98.13% overall alignment rate Time searching: 00:09:05 Overall time: 00:09:05 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 7772729 / 29424702 = 0.2642 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:08:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX152101/SRX152101.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX152101/SRX152101.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX152101/SRX152101.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX152101/SRX152101.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:08:15: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:08:15: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:08:20: 1000000 INFO @ Sun, 21 Jun 2020 18:08:25: 2000000 INFO @ Sun, 21 Jun 2020 18:08:30: 3000000 INFO @ Sun, 21 Jun 2020 18:08:35: 4000000 INFO @ Sun, 21 Jun 2020 18:08:40: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:08:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX152101/SRX152101.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX152101/SRX152101.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX152101/SRX152101.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX152101/SRX152101.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:08:45: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:08:45: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:08:45: 6000000 INFO @ Sun, 21 Jun 2020 18:08:50: 1000000 INFO @ Sun, 21 Jun 2020 18:08:50: 7000000 INFO @ Sun, 21 Jun 2020 18:08:56: 2000000 INFO @ Sun, 21 Jun 2020 18:08:56: 8000000 INFO @ Sun, 21 Jun 2020 18:09:01: 3000000 INFO @ Sun, 21 Jun 2020 18:09:01: 9000000 INFO @ Sun, 21 Jun 2020 18:09:06: 10000000 INFO @ Sun, 21 Jun 2020 18:09:06: 4000000 INFO @ Sun, 21 Jun 2020 18:09:11: 11000000 INFO @ Sun, 21 Jun 2020 18:09:12: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:09:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX152101/SRX152101.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX152101/SRX152101.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX152101/SRX152101.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX152101/SRX152101.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:09:14: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:09:14: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:09:17: 12000000 INFO @ Sun, 21 Jun 2020 18:09:17: 6000000 INFO @ Sun, 21 Jun 2020 18:09:20: 1000000 INFO @ Sun, 21 Jun 2020 18:09:22: 13000000 INFO @ Sun, 21 Jun 2020 18:09:23: 7000000 INFO @ Sun, 21 Jun 2020 18:09:25: 2000000 INFO @ Sun, 21 Jun 2020 18:09:27: 14000000 INFO @ Sun, 21 Jun 2020 18:09:28: 8000000 INFO @ Sun, 21 Jun 2020 18:09:31: 3000000 INFO @ Sun, 21 Jun 2020 18:09:32: 15000000 INFO @ Sun, 21 Jun 2020 18:09:34: 9000000 INFO @ Sun, 21 Jun 2020 18:09:36: 4000000 INFO @ Sun, 21 Jun 2020 18:09:38: 16000000 INFO @ Sun, 21 Jun 2020 18:09:40: 10000000 INFO @ Sun, 21 Jun 2020 18:09:42: 5000000 INFO @ Sun, 21 Jun 2020 18:09:43: 17000000 INFO @ Sun, 21 Jun 2020 18:09:46: 11000000 INFO @ Sun, 21 Jun 2020 18:09:47: 6000000 INFO @ Sun, 21 Jun 2020 18:09:49: 18000000 INFO @ Sun, 21 Jun 2020 18:09:51: 12000000 INFO @ Sun, 21 Jun 2020 18:09:52: 7000000 INFO @ Sun, 21 Jun 2020 18:09:54: 19000000 INFO @ Sun, 21 Jun 2020 18:09:57: 13000000 INFO @ Sun, 21 Jun 2020 18:09:57: 8000000 INFO @ Sun, 21 Jun 2020 18:10:00: 20000000 INFO @ Sun, 21 Jun 2020 18:10:02: 14000000 INFO @ Sun, 21 Jun 2020 18:10:03: 9000000 INFO @ Sun, 21 Jun 2020 18:10:05: 21000000 INFO @ Sun, 21 Jun 2020 18:10:07: 15000000 INFO @ Sun, 21 Jun 2020 18:10:08: 10000000 INFO @ Sun, 21 Jun 2020 18:10:09: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 18:10:09: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 18:10:09: #1 total tags in treatment: 21651973 INFO @ Sun, 21 Jun 2020 18:10:09: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:10:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:10:09: #1 tags after filtering in treatment: 21651971 INFO @ Sun, 21 Jun 2020 18:10:09: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:10:09: #1 finished! INFO @ Sun, 21 Jun 2020 18:10:09: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:10:09: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:10:11: #2 number of paired peaks: 72 WARNING @ Sun, 21 Jun 2020 18:10:11: Too few paired peaks (72) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:10:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX152101/SRX152101.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX152101/SRX152101.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX152101/SRX152101.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX152101/SRX152101.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 18:10:13: 16000000 INFO @ Sun, 21 Jun 2020 18:10:14: 11000000 INFO @ Sun, 21 Jun 2020 18:10:18: 17000000 INFO @ Sun, 21 Jun 2020 18:10:19: 12000000 INFO @ Sun, 21 Jun 2020 18:10:24: 18000000 INFO @ Sun, 21 Jun 2020 18:10:25: 13000000 INFO @ Sun, 21 Jun 2020 18:10:29: 19000000 INFO @ Sun, 21 Jun 2020 18:10:30: 14000000 INFO @ Sun, 21 Jun 2020 18:10:35: 20000000 INFO @ Sun, 21 Jun 2020 18:10:35: 15000000 INFO @ Sun, 21 Jun 2020 18:10:40: 21000000 INFO @ Sun, 21 Jun 2020 18:10:41: 16000000 INFO @ Sun, 21 Jun 2020 18:10:44: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 18:10:44: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 18:10:44: #1 total tags in treatment: 21651973 INFO @ Sun, 21 Jun 2020 18:10:44: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:10:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:10:45: #1 tags after filtering in treatment: 21651971 INFO @ Sun, 21 Jun 2020 18:10:45: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:10:45: #1 finished! INFO @ Sun, 21 Jun 2020 18:10:45: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:10:45: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:10:46: #2 number of paired peaks: 72 WARNING @ Sun, 21 Jun 2020 18:10:46: Too few paired peaks (72) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:10:46: Process for pairing-model is terminated! INFO @ Sun, 21 Jun 2020 18:10:47: 17000000 cut: /home/okishinya/chipatlas/results/dm6/SRX152101/SRX152101.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX152101/SRX152101.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX152101/SRX152101.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX152101/SRX152101.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 18:10:52: 18000000 INFO @ Sun, 21 Jun 2020 18:10:57: 19000000 INFO @ Sun, 21 Jun 2020 18:11:02: 20000000 INFO @ Sun, 21 Jun 2020 18:11:08: 21000000 INFO @ Sun, 21 Jun 2020 18:11:12: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 18:11:12: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 18:11:12: #1 total tags in treatment: 21651973 INFO @ Sun, 21 Jun 2020 18:11:12: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:11:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:11:12: #1 tags after filtering in treatment: 21651971 INFO @ Sun, 21 Jun 2020 18:11:12: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:11:12: #1 finished! INFO @ Sun, 21 Jun 2020 18:11:12: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:11:12: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:11:14: #2 number of paired peaks: 72 WARNING @ Sun, 21 Jun 2020 18:11:14: Too few paired peaks (72) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:11:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX152101/SRX152101.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX152101/SRX152101.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX152101/SRX152101.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX152101/SRX152101.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。