Job ID = 6453838 SRX = SRX152093 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-21T08:42:32 prefetch.2.10.7: 1) Downloading 'SRR504949'... 2020-06-21T08:42:32 prefetch.2.10.7: Downloading via HTTPS... 2020-06-21T09:06:26 prefetch.2.10.7: HTTPS download succeed 2020-06-21T09:06:26 prefetch.2.10.7: 1) 'SRR504949' was downloaded successfully Read 72611177 spots for SRR504949/SRR504949.sra Written 72611177 spots for SRR504949/SRR504949.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:01 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:21:25 72611177 reads; of these: 72611177 (100.00%) were unpaired; of these: 2298028 (3.16%) aligned 0 times 51536545 (70.98%) aligned exactly 1 time 18776604 (25.86%) aligned >1 times 96.84% overall alignment rate Time searching: 00:21:26 Overall time: 00:21:26 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 32 files... [bam_rmdupse_core] 30236844 / 70313149 = 0.4300 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:48:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX152093/SRX152093.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX152093/SRX152093.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX152093/SRX152093.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX152093/SRX152093.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:48:23: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:48:23: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:48:28: 1000000 INFO @ Sun, 21 Jun 2020 18:48:33: 2000000 INFO @ Sun, 21 Jun 2020 18:48:38: 3000000 INFO @ Sun, 21 Jun 2020 18:48:43: 4000000 INFO @ Sun, 21 Jun 2020 18:48:48: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:48:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX152093/SRX152093.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX152093/SRX152093.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX152093/SRX152093.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX152093/SRX152093.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:48:53: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:48:53: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:48:54: 6000000 INFO @ Sun, 21 Jun 2020 18:48:58: 1000000 INFO @ Sun, 21 Jun 2020 18:48:59: 7000000 INFO @ Sun, 21 Jun 2020 18:49:04: 2000000 INFO @ Sun, 21 Jun 2020 18:49:04: 8000000 INFO @ Sun, 21 Jun 2020 18:49:09: 3000000 INFO @ Sun, 21 Jun 2020 18:49:10: 9000000 INFO @ Sun, 21 Jun 2020 18:49:15: 4000000 INFO @ Sun, 21 Jun 2020 18:49:15: 10000000 INFO @ Sun, 21 Jun 2020 18:49:20: 11000000 INFO @ Sun, 21 Jun 2020 18:49:21: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sun, 21 Jun 2020 18:49:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX152093/SRX152093.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX152093/SRX152093.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX152093/SRX152093.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX152093/SRX152093.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 21 Jun 2020 18:49:23: #1 read tag files... INFO @ Sun, 21 Jun 2020 18:49:23: #1 read treatment tags... INFO @ Sun, 21 Jun 2020 18:49:26: 12000000 INFO @ Sun, 21 Jun 2020 18:49:26: 6000000 INFO @ Sun, 21 Jun 2020 18:49:29: 1000000 INFO @ Sun, 21 Jun 2020 18:49:31: 13000000 INFO @ Sun, 21 Jun 2020 18:49:32: 7000000 INFO @ Sun, 21 Jun 2020 18:49:34: 2000000 INFO @ Sun, 21 Jun 2020 18:49:37: 14000000 INFO @ Sun, 21 Jun 2020 18:49:38: 8000000 INFO @ Sun, 21 Jun 2020 18:49:40: 3000000 INFO @ Sun, 21 Jun 2020 18:49:42: 15000000 INFO @ Sun, 21 Jun 2020 18:49:43: 9000000 INFO @ Sun, 21 Jun 2020 18:49:46: 4000000 INFO @ Sun, 21 Jun 2020 18:49:48: 16000000 INFO @ Sun, 21 Jun 2020 18:49:49: 10000000 INFO @ Sun, 21 Jun 2020 18:49:52: 5000000 INFO @ Sun, 21 Jun 2020 18:49:53: 17000000 INFO @ Sun, 21 Jun 2020 18:49:55: 11000000 INFO @ Sun, 21 Jun 2020 18:49:57: 6000000 INFO @ Sun, 21 Jun 2020 18:49:59: 18000000 INFO @ Sun, 21 Jun 2020 18:50:00: 12000000 INFO @ Sun, 21 Jun 2020 18:50:04: 7000000 INFO @ Sun, 21 Jun 2020 18:50:04: 19000000 INFO @ Sun, 21 Jun 2020 18:50:06: 13000000 INFO @ Sun, 21 Jun 2020 18:50:09: 20000000 INFO @ Sun, 21 Jun 2020 18:50:10: 8000000 INFO @ Sun, 21 Jun 2020 18:50:12: 14000000 INFO @ Sun, 21 Jun 2020 18:50:15: 21000000 INFO @ Sun, 21 Jun 2020 18:50:16: 9000000 INFO @ Sun, 21 Jun 2020 18:50:17: 15000000 INFO @ Sun, 21 Jun 2020 18:50:20: 22000000 INFO @ Sun, 21 Jun 2020 18:50:22: 10000000 INFO @ Sun, 21 Jun 2020 18:50:23: 16000000 INFO @ Sun, 21 Jun 2020 18:50:26: 23000000 INFO @ Sun, 21 Jun 2020 18:50:28: 11000000 INFO @ Sun, 21 Jun 2020 18:50:29: 17000000 INFO @ Sun, 21 Jun 2020 18:50:31: 24000000 INFO @ Sun, 21 Jun 2020 18:50:34: 12000000 INFO @ Sun, 21 Jun 2020 18:50:34: 18000000 INFO @ Sun, 21 Jun 2020 18:50:36: 25000000 INFO @ Sun, 21 Jun 2020 18:50:40: 19000000 INFO @ Sun, 21 Jun 2020 18:50:40: 13000000 INFO @ Sun, 21 Jun 2020 18:50:42: 26000000 INFO @ Sun, 21 Jun 2020 18:50:45: 20000000 INFO @ Sun, 21 Jun 2020 18:50:46: 14000000 INFO @ Sun, 21 Jun 2020 18:50:47: 27000000 INFO @ Sun, 21 Jun 2020 18:50:51: 21000000 INFO @ Sun, 21 Jun 2020 18:50:52: 15000000 INFO @ Sun, 21 Jun 2020 18:50:52: 28000000 INFO @ Sun, 21 Jun 2020 18:50:56: 22000000 INFO @ Sun, 21 Jun 2020 18:50:57: 16000000 INFO @ Sun, 21 Jun 2020 18:50:58: 29000000 INFO @ Sun, 21 Jun 2020 18:51:01: 23000000 INFO @ Sun, 21 Jun 2020 18:51:03: 30000000 INFO @ Sun, 21 Jun 2020 18:51:03: 17000000 INFO @ Sun, 21 Jun 2020 18:51:07: 24000000 INFO @ Sun, 21 Jun 2020 18:51:08: 31000000 INFO @ Sun, 21 Jun 2020 18:51:09: 18000000 INFO @ Sun, 21 Jun 2020 18:51:12: 25000000 INFO @ Sun, 21 Jun 2020 18:51:14: 32000000 INFO @ Sun, 21 Jun 2020 18:51:15: 19000000 INFO @ Sun, 21 Jun 2020 18:51:18: 26000000 INFO @ Sun, 21 Jun 2020 18:51:19: 33000000 INFO @ Sun, 21 Jun 2020 18:51:20: 20000000 INFO @ Sun, 21 Jun 2020 18:51:23: 27000000 INFO @ Sun, 21 Jun 2020 18:51:25: 34000000 INFO @ Sun, 21 Jun 2020 18:51:25: 21000000 INFO @ Sun, 21 Jun 2020 18:51:28: 28000000 INFO @ Sun, 21 Jun 2020 18:51:30: 35000000 INFO @ Sun, 21 Jun 2020 18:51:30: 22000000 INFO @ Sun, 21 Jun 2020 18:51:34: 29000000 INFO @ Sun, 21 Jun 2020 18:51:35: 36000000 INFO @ Sun, 21 Jun 2020 18:51:35: 23000000 INFO @ Sun, 21 Jun 2020 18:51:39: 30000000 INFO @ Sun, 21 Jun 2020 18:51:40: 37000000 INFO @ Sun, 21 Jun 2020 18:51:41: 24000000 INFO @ Sun, 21 Jun 2020 18:51:45: 31000000 INFO @ Sun, 21 Jun 2020 18:51:46: 38000000 INFO @ Sun, 21 Jun 2020 18:51:46: 25000000 INFO @ Sun, 21 Jun 2020 18:51:50: 32000000 INFO @ Sun, 21 Jun 2020 18:51:51: 39000000 INFO @ Sun, 21 Jun 2020 18:51:51: 26000000 INFO @ Sun, 21 Jun 2020 18:51:56: 33000000 INFO @ Sun, 21 Jun 2020 18:51:56: 27000000 INFO @ Sun, 21 Jun 2020 18:51:56: 40000000 INFO @ Sun, 21 Jun 2020 18:51:57: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 18:51:57: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 18:51:57: #1 total tags in treatment: 40076305 INFO @ Sun, 21 Jun 2020 18:51:57: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:51:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:51:58: #1 tags after filtering in treatment: 40076304 INFO @ Sun, 21 Jun 2020 18:51:58: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:51:58: #1 finished! INFO @ Sun, 21 Jun 2020 18:51:58: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:51:58: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:52:00: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 18:52:00: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:52:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX152093/SRX152093.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX152093/SRX152093.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX152093/SRX152093.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX152093/SRX152093.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 18:52:01: 28000000 INFO @ Sun, 21 Jun 2020 18:52:01: 34000000 INFO @ Sun, 21 Jun 2020 18:52:06: 29000000 INFO @ Sun, 21 Jun 2020 18:52:06: 35000000 INFO @ Sun, 21 Jun 2020 18:52:11: 30000000 INFO @ Sun, 21 Jun 2020 18:52:12: 36000000 INFO @ Sun, 21 Jun 2020 18:52:16: 31000000 INFO @ Sun, 21 Jun 2020 18:52:17: 37000000 INFO @ Sun, 21 Jun 2020 18:52:22: 32000000 INFO @ Sun, 21 Jun 2020 18:52:22: 38000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sun, 21 Jun 2020 18:52:28: 39000000 INFO @ Sun, 21 Jun 2020 18:52:28: 33000000 INFO @ Sun, 21 Jun 2020 18:52:33: 34000000 INFO @ Sun, 21 Jun 2020 18:52:33: 40000000 INFO @ Sun, 21 Jun 2020 18:52:34: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 18:52:34: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 18:52:34: #1 total tags in treatment: 40076305 INFO @ Sun, 21 Jun 2020 18:52:34: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:52:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:52:35: #1 tags after filtering in treatment: 40076304 INFO @ Sun, 21 Jun 2020 18:52:35: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:52:35: #1 finished! INFO @ Sun, 21 Jun 2020 18:52:35: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:52:35: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:52:37: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 18:52:37: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:52:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX152093/SRX152093.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX152093/SRX152093.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX152093/SRX152093.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX152093/SRX152093.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 21 Jun 2020 18:52:38: 35000000 INFO @ Sun, 21 Jun 2020 18:52:43: 36000000 INFO @ Sun, 21 Jun 2020 18:52:49: 37000000 INFO @ Sun, 21 Jun 2020 18:52:54: 38000000 INFO @ Sun, 21 Jun 2020 18:52:59: 39000000 INFO @ Sun, 21 Jun 2020 18:53:05: 40000000 INFO @ Sun, 21 Jun 2020 18:53:05: #1 tag size is determined as 50 bps INFO @ Sun, 21 Jun 2020 18:53:05: #1 tag size = 50 INFO @ Sun, 21 Jun 2020 18:53:05: #1 total tags in treatment: 40076305 INFO @ Sun, 21 Jun 2020 18:53:05: #1 user defined the maximum tags... INFO @ Sun, 21 Jun 2020 18:53:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 21 Jun 2020 18:53:06: #1 tags after filtering in treatment: 40076304 INFO @ Sun, 21 Jun 2020 18:53:06: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 21 Jun 2020 18:53:06: #1 finished! INFO @ Sun, 21 Jun 2020 18:53:06: #2 Build Peak Model... INFO @ Sun, 21 Jun 2020 18:53:06: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 21 Jun 2020 18:53:09: #2 number of paired peaks: 0 WARNING @ Sun, 21 Jun 2020 18:53:09: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 21 Jun 2020 18:53:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX152093/SRX152093.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX152093/SRX152093.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX152093/SRX152093.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX152093/SRX152093.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。