Job ID = 16438951 SRX = SRX15206511 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:24 14217417 reads; of these: 14217417 (100.00%) were unpaired; of these: 1693185 (11.91%) aligned 0 times 8879212 (62.45%) aligned exactly 1 time 3645020 (25.64%) aligned >1 times 88.09% overall alignment rate Time searching: 00:03:24 Overall time: 00:03:24 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4019691 / 12524232 = 0.3210 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 14:20:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX15206511/SRX15206511.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX15206511/SRX15206511.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX15206511/SRX15206511.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX15206511/SRX15206511.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 14:20:55: #1 read tag files... INFO @ Tue, 02 Aug 2022 14:20:55: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 14:21:01: 1000000 INFO @ Tue, 02 Aug 2022 14:21:07: 2000000 INFO @ Tue, 02 Aug 2022 14:21:12: 3000000 INFO @ Tue, 02 Aug 2022 14:21:17: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 14:21:23: 5000000 INFO @ Tue, 02 Aug 2022 14:21:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX15206511/SRX15206511.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX15206511/SRX15206511.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX15206511/SRX15206511.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX15206511/SRX15206511.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 14:21:25: #1 read tag files... INFO @ Tue, 02 Aug 2022 14:21:25: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 14:21:29: 6000000 INFO @ Tue, 02 Aug 2022 14:21:31: 1000000 INFO @ Tue, 02 Aug 2022 14:21:35: 7000000 INFO @ Tue, 02 Aug 2022 14:21:36: 2000000 INFO @ Tue, 02 Aug 2022 14:21:41: 8000000 INFO @ Tue, 02 Aug 2022 14:21:42: 3000000 INFO @ Tue, 02 Aug 2022 14:21:44: #1 tag size is determined as 50 bps INFO @ Tue, 02 Aug 2022 14:21:44: #1 tag size = 50 INFO @ Tue, 02 Aug 2022 14:21:44: #1 total tags in treatment: 8504541 INFO @ Tue, 02 Aug 2022 14:21:44: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 14:21:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 14:21:45: #1 tags after filtering in treatment: 8504474 INFO @ Tue, 02 Aug 2022 14:21:45: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 14:21:45: #1 finished! INFO @ Tue, 02 Aug 2022 14:21:45: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 14:21:45: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 14:21:46: #2 number of paired peaks: 1134 INFO @ Tue, 02 Aug 2022 14:21:46: start model_add_line... INFO @ Tue, 02 Aug 2022 14:21:46: start X-correlation... INFO @ Tue, 02 Aug 2022 14:21:46: end of X-cor INFO @ Tue, 02 Aug 2022 14:21:46: #2 finished! INFO @ Tue, 02 Aug 2022 14:21:46: #2 predicted fragment length is 160 bps INFO @ Tue, 02 Aug 2022 14:21:46: #2 alternative fragment length(s) may be 160 bps INFO @ Tue, 02 Aug 2022 14:21:46: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX15206511/SRX15206511.05_model.r INFO @ Tue, 02 Aug 2022 14:21:46: #3 Call peaks... INFO @ Tue, 02 Aug 2022 14:21:46: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 14:21:48: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 14:21:53: 5000000 INFO @ Tue, 02 Aug 2022 14:21:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX15206511/SRX15206511.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX15206511/SRX15206511.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX15206511/SRX15206511.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX15206511/SRX15206511.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 14:21:55: #1 read tag files... INFO @ Tue, 02 Aug 2022 14:21:55: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 14:21:59: 6000000 INFO @ Tue, 02 Aug 2022 14:22:01: 1000000 INFO @ Tue, 02 Aug 2022 14:22:06: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 14:22:06: 7000000 INFO @ Tue, 02 Aug 2022 14:22:06: 2000000 INFO @ Tue, 02 Aug 2022 14:22:12: 3000000 INFO @ Tue, 02 Aug 2022 14:22:12: 8000000 INFO @ Tue, 02 Aug 2022 14:22:16: #1 tag size is determined as 50 bps INFO @ Tue, 02 Aug 2022 14:22:16: #1 tag size = 50 INFO @ Tue, 02 Aug 2022 14:22:16: #1 total tags in treatment: 8504541 INFO @ Tue, 02 Aug 2022 14:22:16: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 14:22:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 14:22:16: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX15206511/SRX15206511.05_peaks.xls INFO @ Tue, 02 Aug 2022 14:22:16: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX15206511/SRX15206511.05_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 14:22:16: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX15206511/SRX15206511.05_summits.bed INFO @ Tue, 02 Aug 2022 14:22:16: Done! pass1 - making usageList (314 chroms): 1 millis INFO @ Tue, 02 Aug 2022 14:22:16: #1 tags after filtering in treatment: 8504474 INFO @ Tue, 02 Aug 2022 14:22:16: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 14:22:16: #1 finished! INFO @ Tue, 02 Aug 2022 14:22:16: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 14:22:16: #2 looking for paired plus/minus strand peaks... pass2 - checking and writing primary data (2237 records, 4 fields): 137 millis CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 14:22:17: 4000000 INFO @ Tue, 02 Aug 2022 14:22:17: #2 number of paired peaks: 1134 INFO @ Tue, 02 Aug 2022 14:22:17: start model_add_line... INFO @ Tue, 02 Aug 2022 14:22:17: start X-correlation... INFO @ Tue, 02 Aug 2022 14:22:17: end of X-cor INFO @ Tue, 02 Aug 2022 14:22:17: #2 finished! INFO @ Tue, 02 Aug 2022 14:22:17: #2 predicted fragment length is 160 bps INFO @ Tue, 02 Aug 2022 14:22:17: #2 alternative fragment length(s) may be 160 bps INFO @ Tue, 02 Aug 2022 14:22:17: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX15206511/SRX15206511.10_model.r INFO @ Tue, 02 Aug 2022 14:22:17: #3 Call peaks... INFO @ Tue, 02 Aug 2022 14:22:17: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 14:22:22: 5000000 INFO @ Tue, 02 Aug 2022 14:22:28: 6000000 INFO @ Tue, 02 Aug 2022 14:22:34: 7000000 INFO @ Tue, 02 Aug 2022 14:22:38: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 14:22:39: 8000000 INFO @ Tue, 02 Aug 2022 14:22:42: #1 tag size is determined as 50 bps INFO @ Tue, 02 Aug 2022 14:22:42: #1 tag size = 50 INFO @ Tue, 02 Aug 2022 14:22:42: #1 total tags in treatment: 8504541 INFO @ Tue, 02 Aug 2022 14:22:42: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 14:22:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 14:22:43: #1 tags after filtering in treatment: 8504474 INFO @ Tue, 02 Aug 2022 14:22:43: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 14:22:43: #1 finished! INFO @ Tue, 02 Aug 2022 14:22:43: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 14:22:43: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 14:22:44: #2 number of paired peaks: 1134 INFO @ Tue, 02 Aug 2022 14:22:44: start model_add_line... INFO @ Tue, 02 Aug 2022 14:22:44: start X-correlation... INFO @ Tue, 02 Aug 2022 14:22:44: end of X-cor INFO @ Tue, 02 Aug 2022 14:22:44: #2 finished! INFO @ Tue, 02 Aug 2022 14:22:44: #2 predicted fragment length is 160 bps INFO @ Tue, 02 Aug 2022 14:22:44: #2 alternative fragment length(s) may be 160 bps INFO @ Tue, 02 Aug 2022 14:22:44: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX15206511/SRX15206511.20_model.r INFO @ Tue, 02 Aug 2022 14:22:44: #3 Call peaks... INFO @ Tue, 02 Aug 2022 14:22:44: #3 Pre-compute pvalue-qvalue table... BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 14:22:47: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX15206511/SRX15206511.10_peaks.xls INFO @ Tue, 02 Aug 2022 14:22:47: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX15206511/SRX15206511.10_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 14:22:47: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX15206511/SRX15206511.10_summits.bed INFO @ Tue, 02 Aug 2022 14:22:47: Done! pass1 - making usageList (262 chroms): 1 millis pass2 - checking and writing primary data (1134 records, 4 fields): 467 millis CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 14:23:03: #3 Call peaks for each chromosome... BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 14:23:13: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX15206511/SRX15206511.20_peaks.xls INFO @ Tue, 02 Aug 2022 14:23:13: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX15206511/SRX15206511.20_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 14:23:13: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX15206511/SRX15206511.20_summits.bed INFO @ Tue, 02 Aug 2022 14:23:13: Done! pass1 - making usageList (182 chroms): 1 millis pass2 - checking and writing primary data (536 records, 4 fields): 46 millis CompletedMACS2peakCalling