Job ID = 16439354 SRX = SRX15206493 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:57 7700578 reads; of these: 7700578 (100.00%) were unpaired; of these: 295233 (3.83%) aligned 0 times 6849234 (88.94%) aligned exactly 1 time 556111 (7.22%) aligned >1 times 96.17% overall alignment rate Time searching: 00:02:57 Overall time: 00:02:57 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_rmdupse_core] 1694246 / 7405345 = 0.2288 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:03:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX15206493/SRX15206493.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX15206493/SRX15206493.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX15206493/SRX15206493.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX15206493/SRX15206493.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:03:38: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:03:38: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:03:49: 1000000 INFO @ Tue, 02 Aug 2022 15:04:02: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:04:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX15206493/SRX15206493.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX15206493/SRX15206493.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX15206493/SRX15206493.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX15206493/SRX15206493.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:04:08: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:04:08: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:04:14: 3000000 INFO @ Tue, 02 Aug 2022 15:04:19: 1000000 INFO @ Tue, 02 Aug 2022 15:04:27: 4000000 INFO @ Tue, 02 Aug 2022 15:04:32: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:04:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX15206493/SRX15206493.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX15206493/SRX15206493.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX15206493/SRX15206493.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX15206493/SRX15206493.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:04:38: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:04:38: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:04:39: 5000000 INFO @ Tue, 02 Aug 2022 15:04:44: 3000000 INFO @ Tue, 02 Aug 2022 15:04:46: 1000000 INFO @ Tue, 02 Aug 2022 15:04:47: #1 tag size is determined as 75 bps INFO @ Tue, 02 Aug 2022 15:04:47: #1 tag size = 75 INFO @ Tue, 02 Aug 2022 15:04:47: #1 total tags in treatment: 5711099 INFO @ Tue, 02 Aug 2022 15:04:47: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:04:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:04:47: #1 tags after filtering in treatment: 5710757 INFO @ Tue, 02 Aug 2022 15:04:47: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:04:47: #1 finished! INFO @ Tue, 02 Aug 2022 15:04:47: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:04:47: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:04:48: #2 number of paired peaks: 7011 INFO @ Tue, 02 Aug 2022 15:04:48: start model_add_line... INFO @ Tue, 02 Aug 2022 15:04:49: start X-correlation... INFO @ Tue, 02 Aug 2022 15:04:49: end of X-cor INFO @ Tue, 02 Aug 2022 15:04:49: #2 finished! INFO @ Tue, 02 Aug 2022 15:04:49: #2 predicted fragment length is 199 bps INFO @ Tue, 02 Aug 2022 15:04:49: #2 alternative fragment length(s) may be 199 bps INFO @ Tue, 02 Aug 2022 15:04:49: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX15206493/SRX15206493.05_model.r INFO @ Tue, 02 Aug 2022 15:04:49: #3 Call peaks... INFO @ Tue, 02 Aug 2022 15:04:49: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 15:04:54: 2000000 INFO @ Tue, 02 Aug 2022 15:04:55: 4000000 INFO @ Tue, 02 Aug 2022 15:05:02: 3000000 INFO @ Tue, 02 Aug 2022 15:05:06: 5000000 INFO @ Tue, 02 Aug 2022 15:05:10: 4000000 INFO @ Tue, 02 Aug 2022 15:05:12: #3 Call peaks for each chromosome... BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 15:05:13: #1 tag size is determined as 75 bps INFO @ Tue, 02 Aug 2022 15:05:13: #1 tag size = 75 INFO @ Tue, 02 Aug 2022 15:05:13: #1 total tags in treatment: 5711099 INFO @ Tue, 02 Aug 2022 15:05:13: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:05:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:05:13: #1 tags after filtering in treatment: 5710757 INFO @ Tue, 02 Aug 2022 15:05:13: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:05:13: #1 finished! INFO @ Tue, 02 Aug 2022 15:05:13: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:05:13: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:05:15: #2 number of paired peaks: 7011 INFO @ Tue, 02 Aug 2022 15:05:15: start model_add_line... INFO @ Tue, 02 Aug 2022 15:05:15: start X-correlation... INFO @ Tue, 02 Aug 2022 15:05:15: end of X-cor INFO @ Tue, 02 Aug 2022 15:05:15: #2 finished! INFO @ Tue, 02 Aug 2022 15:05:15: #2 predicted fragment length is 199 bps INFO @ Tue, 02 Aug 2022 15:05:15: #2 alternative fragment length(s) may be 199 bps INFO @ Tue, 02 Aug 2022 15:05:15: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX15206493/SRX15206493.10_model.r INFO @ Tue, 02 Aug 2022 15:05:15: #3 Call peaks... INFO @ Tue, 02 Aug 2022 15:05:15: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 15:05:19: 5000000 INFO @ Tue, 02 Aug 2022 15:05:23: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX15206493/SRX15206493.05_peaks.xls INFO @ Tue, 02 Aug 2022 15:05:24: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX15206493/SRX15206493.05_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 15:05:24: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX15206493/SRX15206493.05_summits.bed INFO @ Tue, 02 Aug 2022 15:05:24: Done! pass1 - making usageList (87 chroms): 5 millis pass2 - checking and writing primary data (7789 records, 4 fields): 44 millis CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 15:05:25: #1 tag size is determined as 75 bps INFO @ Tue, 02 Aug 2022 15:05:25: #1 tag size = 75 INFO @ Tue, 02 Aug 2022 15:05:25: #1 total tags in treatment: 5711099 INFO @ Tue, 02 Aug 2022 15:05:25: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:05:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:05:26: #1 tags after filtering in treatment: 5710757 INFO @ Tue, 02 Aug 2022 15:05:26: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:05:26: #1 finished! INFO @ Tue, 02 Aug 2022 15:05:26: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:05:26: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:05:27: #2 number of paired peaks: 7011 INFO @ Tue, 02 Aug 2022 15:05:27: start model_add_line... INFO @ Tue, 02 Aug 2022 15:05:27: start X-correlation... INFO @ Tue, 02 Aug 2022 15:05:27: end of X-cor INFO @ Tue, 02 Aug 2022 15:05:27: #2 finished! INFO @ Tue, 02 Aug 2022 15:05:27: #2 predicted fragment length is 199 bps INFO @ Tue, 02 Aug 2022 15:05:27: #2 alternative fragment length(s) may be 199 bps INFO @ Tue, 02 Aug 2022 15:05:27: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX15206493/SRX15206493.20_model.r INFO @ Tue, 02 Aug 2022 15:05:27: #3 Call peaks... INFO @ Tue, 02 Aug 2022 15:05:27: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 15:05:39: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 15:05:51: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX15206493/SRX15206493.10_peaks.xls INFO @ Tue, 02 Aug 2022 15:05:51: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX15206493/SRX15206493.10_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 15:05:51: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX15206493/SRX15206493.10_summits.bed INFO @ Tue, 02 Aug 2022 15:05:51: Done! pass1 - making usageList (62 chroms): 5 millis pass2 - checking and writing primary data (6151 records, 4 fields): 63 millis CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 15:05:53: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 15:06:04: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX15206493/SRX15206493.20_peaks.xls INFO @ Tue, 02 Aug 2022 15:06:04: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX15206493/SRX15206493.20_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 15:06:04: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX15206493/SRX15206493.20_summits.bed INFO @ Tue, 02 Aug 2022 15:06:04: Done! pass1 - making usageList (43 chroms): 2 millis pass2 - checking and writing primary data (4491 records, 4 fields): 38 millis CompletedMACS2peakCalling