Job ID = 16439361 SRX = SRX15206488 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:20 11694360 reads; of these: 11694360 (100.00%) were unpaired; of these: 498185 (4.26%) aligned 0 times 9535564 (81.54%) aligned exactly 1 time 1660611 (14.20%) aligned >1 times 95.74% overall alignment rate Time searching: 00:05:21 Overall time: 00:05:21 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2259079 / 11196175 = 0.2018 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:08:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX15206488/SRX15206488.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX15206488/SRX15206488.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX15206488/SRX15206488.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX15206488/SRX15206488.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:08:40: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:08:40: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:08:53: 1000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:09:06: 2000000 INFO @ Tue, 02 Aug 2022 15:09:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX15206488/SRX15206488.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX15206488/SRX15206488.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX15206488/SRX15206488.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX15206488/SRX15206488.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:09:08: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:09:08: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:09:19: 3000000 INFO @ Tue, 02 Aug 2022 15:09:21: 1000000 INFO @ Tue, 02 Aug 2022 15:09:32: 4000000 INFO @ Tue, 02 Aug 2022 15:09:33: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:09:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX15206488/SRX15206488.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX15206488/SRX15206488.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX15206488/SRX15206488.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX15206488/SRX15206488.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:09:38: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:09:38: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:09:45: 5000000 INFO @ Tue, 02 Aug 2022 15:09:46: 3000000 INFO @ Tue, 02 Aug 2022 15:09:48: 1000000 INFO @ Tue, 02 Aug 2022 15:09:58: 2000000 INFO @ Tue, 02 Aug 2022 15:09:59: 6000000 INFO @ Tue, 02 Aug 2022 15:09:59: 4000000 INFO @ Tue, 02 Aug 2022 15:10:08: 3000000 INFO @ Tue, 02 Aug 2022 15:10:12: 7000000 INFO @ Tue, 02 Aug 2022 15:10:13: 5000000 INFO @ Tue, 02 Aug 2022 15:10:17: 4000000 INFO @ Tue, 02 Aug 2022 15:10:25: 6000000 INFO @ Tue, 02 Aug 2022 15:10:26: 8000000 INFO @ Tue, 02 Aug 2022 15:10:27: 5000000 INFO @ Tue, 02 Aug 2022 15:10:37: 6000000 INFO @ Tue, 02 Aug 2022 15:10:39: 7000000 INFO @ Tue, 02 Aug 2022 15:10:39: #1 tag size is determined as 75 bps INFO @ Tue, 02 Aug 2022 15:10:39: #1 tag size = 75 INFO @ Tue, 02 Aug 2022 15:10:39: #1 total tags in treatment: 8937096 INFO @ Tue, 02 Aug 2022 15:10:39: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:10:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:10:40: #1 tags after filtering in treatment: 8936888 INFO @ Tue, 02 Aug 2022 15:10:40: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:10:40: #1 finished! INFO @ Tue, 02 Aug 2022 15:10:40: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:10:40: #2 looking for paired plus/minus strand peaks... BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 15:10:41: #2 number of paired peaks: 4373 INFO @ Tue, 02 Aug 2022 15:10:41: start model_add_line... INFO @ Tue, 02 Aug 2022 15:10:41: start X-correlation... INFO @ Tue, 02 Aug 2022 15:10:41: end of X-cor INFO @ Tue, 02 Aug 2022 15:10:41: #2 finished! INFO @ Tue, 02 Aug 2022 15:10:41: #2 predicted fragment length is 157 bps INFO @ Tue, 02 Aug 2022 15:10:41: #2 alternative fragment length(s) may be 157 bps INFO @ Tue, 02 Aug 2022 15:10:41: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX15206488/SRX15206488.05_model.r INFO @ Tue, 02 Aug 2022 15:10:41: #3 Call peaks... INFO @ Tue, 02 Aug 2022 15:10:41: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 15:10:46: 7000000 INFO @ Tue, 02 Aug 2022 15:10:52: 8000000 INFO @ Tue, 02 Aug 2022 15:10:56: 8000000 INFO @ Tue, 02 Aug 2022 15:11:04: #1 tag size is determined as 75 bps INFO @ Tue, 02 Aug 2022 15:11:04: #1 tag size = 75 INFO @ Tue, 02 Aug 2022 15:11:04: #1 total tags in treatment: 8937096 INFO @ Tue, 02 Aug 2022 15:11:04: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:11:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:11:05: #1 tags after filtering in treatment: 8936888 INFO @ Tue, 02 Aug 2022 15:11:05: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:11:05: #1 finished! INFO @ Tue, 02 Aug 2022 15:11:05: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:11:05: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:11:06: #2 number of paired peaks: 4373 INFO @ Tue, 02 Aug 2022 15:11:06: start model_add_line... INFO @ Tue, 02 Aug 2022 15:11:06: start X-correlation... INFO @ Tue, 02 Aug 2022 15:11:06: end of X-cor INFO @ Tue, 02 Aug 2022 15:11:06: #2 finished! INFO @ Tue, 02 Aug 2022 15:11:06: #2 predicted fragment length is 157 bps INFO @ Tue, 02 Aug 2022 15:11:06: #2 alternative fragment length(s) may be 157 bps INFO @ Tue, 02 Aug 2022 15:11:06: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX15206488/SRX15206488.10_model.r INFO @ Tue, 02 Aug 2022 15:11:06: #3 Call peaks... INFO @ Tue, 02 Aug 2022 15:11:06: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 15:11:06: #1 tag size is determined as 75 bps INFO @ Tue, 02 Aug 2022 15:11:06: #1 tag size = 75 INFO @ Tue, 02 Aug 2022 15:11:06: #1 total tags in treatment: 8937096 INFO @ Tue, 02 Aug 2022 15:11:06: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:11:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:11:07: #1 tags after filtering in treatment: 8936888 INFO @ Tue, 02 Aug 2022 15:11:07: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:11:07: #1 finished! INFO @ Tue, 02 Aug 2022 15:11:07: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:11:07: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:11:08: #2 number of paired peaks: 4373 INFO @ Tue, 02 Aug 2022 15:11:08: start model_add_line... INFO @ Tue, 02 Aug 2022 15:11:08: start X-correlation... INFO @ Tue, 02 Aug 2022 15:11:08: end of X-cor INFO @ Tue, 02 Aug 2022 15:11:08: #2 finished! INFO @ Tue, 02 Aug 2022 15:11:08: #2 predicted fragment length is 157 bps INFO @ Tue, 02 Aug 2022 15:11:08: #2 alternative fragment length(s) may be 157 bps INFO @ Tue, 02 Aug 2022 15:11:08: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX15206488/SRX15206488.20_model.r INFO @ Tue, 02 Aug 2022 15:11:08: #3 Call peaks... INFO @ Tue, 02 Aug 2022 15:11:08: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 15:11:16: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 15:11:34: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX15206488/SRX15206488.05_peaks.xls INFO @ Tue, 02 Aug 2022 15:11:34: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX15206488/SRX15206488.05_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 15:11:35: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX15206488/SRX15206488.05_summits.bed INFO @ Tue, 02 Aug 2022 15:11:35: Done! pass1 - making usageList (292 chroms): 5 millis pass2 - checking and writing primary data (14001 records, 4 fields): 47 millis CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 15:11:42: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 15:11:42: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 15:11:59: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX15206488/SRX15206488.20_peaks.xls INFO @ Tue, 02 Aug 2022 15:11:59: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX15206488/SRX15206488.20_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 15:11:59: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX15206488/SRX15206488.20_summits.bed INFO @ Tue, 02 Aug 2022 15:11:59: Done! pass1 - making usageList (174 chroms): 4 millis pass2 - checking and writing primary data (5669 records, 4 fields): 45 millis CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 15:12:02: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX15206488/SRX15206488.10_peaks.xls INFO @ Tue, 02 Aug 2022 15:12:02: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX15206488/SRX15206488.10_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 15:12:03: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX15206488/SRX15206488.10_summits.bed INFO @ Tue, 02 Aug 2022 15:12:03: Done! pass1 - making usageList (214 chroms): 4 millis pass2 - checking and writing primary data (9362 records, 4 fields): 38 millis CompletedMACS2peakCalling