Job ID = 16439287 SRX = SRX15206463 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:38 9254970 reads; of these: 9254970 (100.00%) were unpaired; of these: 265519 (2.87%) aligned 0 times 3171711 (34.27%) aligned exactly 1 time 5817740 (62.86%) aligned >1 times 97.13% overall alignment rate Time searching: 00:03:39 Overall time: 00:03:39 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_rmdupse_core] 1634143 / 8989451 = 0.1818 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 14:57:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX15206463/SRX15206463.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX15206463/SRX15206463.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX15206463/SRX15206463.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX15206463/SRX15206463.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 14:57:12: #1 read tag files... INFO @ Tue, 02 Aug 2022 14:57:12: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 14:57:21: 1000000 INFO @ Tue, 02 Aug 2022 14:57:31: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 14:57:41: 3000000 INFO @ Tue, 02 Aug 2022 14:57:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX15206463/SRX15206463.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX15206463/SRX15206463.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX15206463/SRX15206463.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX15206463/SRX15206463.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 14:57:42: #1 read tag files... INFO @ Tue, 02 Aug 2022 14:57:42: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 14:57:50: 4000000 INFO @ Tue, 02 Aug 2022 14:57:52: 1000000 INFO @ Tue, 02 Aug 2022 14:57:59: 5000000 INFO @ Tue, 02 Aug 2022 14:58:02: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 14:58:10: 6000000 INFO @ Tue, 02 Aug 2022 14:58:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX15206463/SRX15206463.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX15206463/SRX15206463.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX15206463/SRX15206463.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX15206463/SRX15206463.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 14:58:12: #1 read tag files... INFO @ Tue, 02 Aug 2022 14:58:12: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 14:58:13: 3000000 INFO @ Tue, 02 Aug 2022 14:58:20: 7000000 INFO @ Tue, 02 Aug 2022 14:58:22: 1000000 INFO @ Tue, 02 Aug 2022 14:58:23: 4000000 INFO @ Tue, 02 Aug 2022 14:58:24: #1 tag size is determined as 50 bps INFO @ Tue, 02 Aug 2022 14:58:24: #1 tag size = 50 INFO @ Tue, 02 Aug 2022 14:58:24: #1 total tags in treatment: 7355308 INFO @ Tue, 02 Aug 2022 14:58:24: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 14:58:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 14:58:25: #1 tags after filtering in treatment: 7355258 INFO @ Tue, 02 Aug 2022 14:58:25: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 14:58:25: #1 finished! INFO @ Tue, 02 Aug 2022 14:58:25: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 14:58:25: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 14:58:26: #2 number of paired peaks: 8037 INFO @ Tue, 02 Aug 2022 14:58:26: start model_add_line... INFO @ Tue, 02 Aug 2022 14:58:26: start X-correlation... INFO @ Tue, 02 Aug 2022 14:58:26: end of X-cor INFO @ Tue, 02 Aug 2022 14:58:26: #2 finished! INFO @ Tue, 02 Aug 2022 14:58:26: #2 predicted fragment length is 193 bps INFO @ Tue, 02 Aug 2022 14:58:26: #2 alternative fragment length(s) may be 3,193 bps INFO @ Tue, 02 Aug 2022 14:58:26: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX15206463/SRX15206463.05_model.r INFO @ Tue, 02 Aug 2022 14:58:26: #3 Call peaks... INFO @ Tue, 02 Aug 2022 14:58:26: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 14:58:32: 2000000 INFO @ Tue, 02 Aug 2022 14:58:32: 5000000 INFO @ Tue, 02 Aug 2022 14:58:43: 3000000 INFO @ Tue, 02 Aug 2022 14:58:43: 6000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 14:58:52: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 14:58:53: 4000000 INFO @ Tue, 02 Aug 2022 14:58:53: 7000000 INFO @ Tue, 02 Aug 2022 14:58:57: #1 tag size is determined as 50 bps INFO @ Tue, 02 Aug 2022 14:58:57: #1 tag size = 50 INFO @ Tue, 02 Aug 2022 14:58:57: #1 total tags in treatment: 7355308 INFO @ Tue, 02 Aug 2022 14:58:57: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 14:58:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 14:58:57: #1 tags after filtering in treatment: 7355258 INFO @ Tue, 02 Aug 2022 14:58:57: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 14:58:57: #1 finished! INFO @ Tue, 02 Aug 2022 14:58:57: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 14:58:57: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 14:58:59: #2 number of paired peaks: 8037 INFO @ Tue, 02 Aug 2022 14:58:59: start model_add_line... INFO @ Tue, 02 Aug 2022 14:58:59: start X-correlation... INFO @ Tue, 02 Aug 2022 14:58:59: end of X-cor INFO @ Tue, 02 Aug 2022 14:58:59: #2 finished! INFO @ Tue, 02 Aug 2022 14:58:59: #2 predicted fragment length is 193 bps INFO @ Tue, 02 Aug 2022 14:58:59: #2 alternative fragment length(s) may be 3,193 bps INFO @ Tue, 02 Aug 2022 14:58:59: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX15206463/SRX15206463.10_model.r INFO @ Tue, 02 Aug 2022 14:58:59: #3 Call peaks... INFO @ Tue, 02 Aug 2022 14:58:59: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 14:59:03: 5000000 INFO @ Tue, 02 Aug 2022 14:59:03: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX15206463/SRX15206463.05_peaks.xls INFO @ Tue, 02 Aug 2022 14:59:04: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX15206463/SRX15206463.05_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 14:59:04: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX15206463/SRX15206463.05_summits.bed INFO @ Tue, 02 Aug 2022 14:59:04: Done! pass1 - making usageList (949 chroms): 4 millis pass2 - checking and writing primary data (3641 records, 4 fields): 42 millis CompletedMACS2peakCalling BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 14:59:14: 6000000 INFO @ Tue, 02 Aug 2022 14:59:24: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 14:59:25: 7000000 INFO @ Tue, 02 Aug 2022 14:59:29: #1 tag size is determined as 50 bps INFO @ Tue, 02 Aug 2022 14:59:29: #1 tag size = 50 INFO @ Tue, 02 Aug 2022 14:59:29: #1 total tags in treatment: 7355308 INFO @ Tue, 02 Aug 2022 14:59:29: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 14:59:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 14:59:29: #1 tags after filtering in treatment: 7355258 INFO @ Tue, 02 Aug 2022 14:59:29: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 14:59:29: #1 finished! INFO @ Tue, 02 Aug 2022 14:59:29: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 14:59:29: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 14:59:31: #2 number of paired peaks: 8037 INFO @ Tue, 02 Aug 2022 14:59:31: start model_add_line... INFO @ Tue, 02 Aug 2022 14:59:31: start X-correlation... INFO @ Tue, 02 Aug 2022 14:59:31: end of X-cor INFO @ Tue, 02 Aug 2022 14:59:31: #2 finished! INFO @ Tue, 02 Aug 2022 14:59:31: #2 predicted fragment length is 193 bps INFO @ Tue, 02 Aug 2022 14:59:31: #2 alternative fragment length(s) may be 3,193 bps INFO @ Tue, 02 Aug 2022 14:59:31: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX15206463/SRX15206463.20_model.r INFO @ Tue, 02 Aug 2022 14:59:31: #3 Call peaks... INFO @ Tue, 02 Aug 2022 14:59:31: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 14:59:36: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX15206463/SRX15206463.10_peaks.xls INFO @ Tue, 02 Aug 2022 14:59:36: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX15206463/SRX15206463.10_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 14:59:37: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX15206463/SRX15206463.10_summits.bed INFO @ Tue, 02 Aug 2022 14:59:37: Done! pass1 - making usageList (763 chroms): 3 millis pass2 - checking and writing primary data (1959 records, 4 fields): 51 millis CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 14:59:57: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 15:00:08: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX15206463/SRX15206463.20_peaks.xls INFO @ Tue, 02 Aug 2022 15:00:09: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX15206463/SRX15206463.20_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 15:00:09: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX15206463/SRX15206463.20_summits.bed INFO @ Tue, 02 Aug 2022 15:00:09: Done! pass1 - making usageList (573 chroms): 2 millis pass2 - checking and writing primary data (1086 records, 4 fields): 48 millis CompletedMACS2peakCalling