Job ID = 16439020 SRX = SRX15206429 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:27 8827577 reads; of these: 8827577 (100.00%) were unpaired; of these: 512693 (5.81%) aligned 0 times 5552291 (62.90%) aligned exactly 1 time 2762593 (31.30%) aligned >1 times 94.19% overall alignment rate Time searching: 00:02:27 Overall time: 00:02:27 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_rmdupse_core] 1713288 / 8314884 = 0.2061 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 14:30:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX15206429/SRX15206429.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX15206429/SRX15206429.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX15206429/SRX15206429.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX15206429/SRX15206429.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 14:30:13: #1 read tag files... INFO @ Tue, 02 Aug 2022 14:30:13: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 14:30:19: 1000000 INFO @ Tue, 02 Aug 2022 14:30:24: 2000000 INFO @ Tue, 02 Aug 2022 14:30:29: 3000000 INFO @ Tue, 02 Aug 2022 14:30:35: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 14:30:41: 5000000 INFO @ Tue, 02 Aug 2022 14:30:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX15206429/SRX15206429.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX15206429/SRX15206429.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX15206429/SRX15206429.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX15206429/SRX15206429.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 14:30:42: #1 read tag files... INFO @ Tue, 02 Aug 2022 14:30:42: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 14:30:46: 6000000 INFO @ Tue, 02 Aug 2022 14:30:48: 1000000 INFO @ Tue, 02 Aug 2022 14:30:50: #1 tag size is determined as 50 bps INFO @ Tue, 02 Aug 2022 14:30:50: #1 tag size = 50 INFO @ Tue, 02 Aug 2022 14:30:50: #1 total tags in treatment: 6601596 INFO @ Tue, 02 Aug 2022 14:30:50: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 14:30:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 14:30:50: #1 tags after filtering in treatment: 6601479 INFO @ Tue, 02 Aug 2022 14:30:50: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 14:30:50: #1 finished! INFO @ Tue, 02 Aug 2022 14:30:50: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 14:30:50: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 14:30:51: #2 number of paired peaks: 4226 INFO @ Tue, 02 Aug 2022 14:30:51: start model_add_line... INFO @ Tue, 02 Aug 2022 14:30:51: start X-correlation... INFO @ Tue, 02 Aug 2022 14:30:51: end of X-cor INFO @ Tue, 02 Aug 2022 14:30:51: #2 finished! INFO @ Tue, 02 Aug 2022 14:30:51: #2 predicted fragment length is 227 bps INFO @ Tue, 02 Aug 2022 14:30:51: #2 alternative fragment length(s) may be 227 bps INFO @ Tue, 02 Aug 2022 14:30:51: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX15206429/SRX15206429.05_model.r INFO @ Tue, 02 Aug 2022 14:30:51: #3 Call peaks... INFO @ Tue, 02 Aug 2022 14:30:51: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 14:30:54: 2000000 INFO @ Tue, 02 Aug 2022 14:31:00: 3000000 INFO @ Tue, 02 Aug 2022 14:31:06: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 14:31:11: 5000000 INFO @ Tue, 02 Aug 2022 14:31:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX15206429/SRX15206429.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX15206429/SRX15206429.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX15206429/SRX15206429.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX15206429/SRX15206429.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 14:31:12: #1 read tag files... INFO @ Tue, 02 Aug 2022 14:31:12: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 14:31:12: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 14:31:17: 6000000 INFO @ Tue, 02 Aug 2022 14:31:18: 1000000 INFO @ Tue, 02 Aug 2022 14:31:21: #1 tag size is determined as 50 bps INFO @ Tue, 02 Aug 2022 14:31:21: #1 tag size = 50 INFO @ Tue, 02 Aug 2022 14:31:21: #1 total tags in treatment: 6601596 INFO @ Tue, 02 Aug 2022 14:31:21: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 14:31:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 14:31:21: #1 tags after filtering in treatment: 6601479 INFO @ Tue, 02 Aug 2022 14:31:21: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 14:31:21: #1 finished! INFO @ Tue, 02 Aug 2022 14:31:21: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 14:31:21: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 14:31:21: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX15206429/SRX15206429.05_peaks.xls INFO @ Tue, 02 Aug 2022 14:31:21: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX15206429/SRX15206429.05_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 14:31:21: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX15206429/SRX15206429.05_summits.bed INFO @ Tue, 02 Aug 2022 14:31:21: Done! INFO @ Tue, 02 Aug 2022 14:31:22: #2 number of paired peaks: 4226 INFO @ Tue, 02 Aug 2022 14:31:22: start model_add_line... INFO @ Tue, 02 Aug 2022 14:31:22: start X-correlation... INFO @ Tue, 02 Aug 2022 14:31:22: end of X-cor INFO @ Tue, 02 Aug 2022 14:31:22: #2 finished! INFO @ Tue, 02 Aug 2022 14:31:22: #2 predicted fragment length is 227 bps INFO @ Tue, 02 Aug 2022 14:31:22: #2 alternative fragment length(s) may be 227 bps INFO @ Tue, 02 Aug 2022 14:31:22: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX15206429/SRX15206429.10_model.r INFO @ Tue, 02 Aug 2022 14:31:22: #3 Call peaks... INFO @ Tue, 02 Aug 2022 14:31:22: #3 Pre-compute pvalue-qvalue table... pass1 - making usageList (417 chroms): 1 millis pass2 - checking and writing primary data (6872 records, 4 fields): 78 millis CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 14:31:24: 2000000 INFO @ Tue, 02 Aug 2022 14:31:30: 3000000 INFO @ Tue, 02 Aug 2022 14:31:35: 4000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 14:31:41: 5000000 INFO @ Tue, 02 Aug 2022 14:31:42: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 14:31:47: 6000000 INFO @ Tue, 02 Aug 2022 14:31:50: #1 tag size is determined as 50 bps INFO @ Tue, 02 Aug 2022 14:31:50: #1 tag size = 50 INFO @ Tue, 02 Aug 2022 14:31:50: #1 total tags in treatment: 6601596 INFO @ Tue, 02 Aug 2022 14:31:50: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 14:31:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 14:31:50: #1 tags after filtering in treatment: 6601479 INFO @ Tue, 02 Aug 2022 14:31:50: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 14:31:50: #1 finished! INFO @ Tue, 02 Aug 2022 14:31:50: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 14:31:50: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 14:31:51: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX15206429/SRX15206429.10_peaks.xls INFO @ Tue, 02 Aug 2022 14:31:51: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX15206429/SRX15206429.10_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 14:31:51: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX15206429/SRX15206429.10_summits.bed INFO @ Tue, 02 Aug 2022 14:31:51: Done! INFO @ Tue, 02 Aug 2022 14:31:51: #2 number of paired peaks: 4226 INFO @ Tue, 02 Aug 2022 14:31:51: start model_add_line... INFO @ Tue, 02 Aug 2022 14:31:51: start X-correlation... INFO @ Tue, 02 Aug 2022 14:31:51: end of X-cor INFO @ Tue, 02 Aug 2022 14:31:51: #2 finished! INFO @ Tue, 02 Aug 2022 14:31:51: #2 predicted fragment length is 227 bps INFO @ Tue, 02 Aug 2022 14:31:51: #2 alternative fragment length(s) may be 227 bps INFO @ Tue, 02 Aug 2022 14:31:51: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX15206429/SRX15206429.20_model.r INFO @ Tue, 02 Aug 2022 14:31:51: #3 Call peaks... INFO @ Tue, 02 Aug 2022 14:31:51: #3 Pre-compute pvalue-qvalue table... pass1 - making usageList (341 chroms): 1 millis pass2 - checking and writing primary data (3824 records, 4 fields): 52 millis CompletedMACS2peakCalling BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 14:32:11: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 14:32:20: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX15206429/SRX15206429.20_peaks.xls INFO @ Tue, 02 Aug 2022 14:32:20: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX15206429/SRX15206429.20_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 14:32:20: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX15206429/SRX15206429.20_summits.bed INFO @ Tue, 02 Aug 2022 14:32:20: Done! pass1 - making usageList (213 chroms): 1 millis pass2 - checking and writing primary data (1498 records, 4 fields): 29 millis CompletedMACS2peakCalling