Job ID = 16439130 SRX = SRX15206427 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:48 13608661 reads; of these: 13608661 (100.00%) were unpaired; of these: 314410 (2.31%) aligned 0 times 8374243 (61.54%) aligned exactly 1 time 4920008 (36.15%) aligned >1 times 97.69% overall alignment rate Time searching: 00:04:48 Overall time: 00:04:48 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 5058688 / 13294251 = 0.3805 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 14:43:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX15206427/SRX15206427.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX15206427/SRX15206427.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX15206427/SRX15206427.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX15206427/SRX15206427.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 14:43:30: #1 read tag files... INFO @ Tue, 02 Aug 2022 14:43:30: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 14:43:35: 1000000 INFO @ Tue, 02 Aug 2022 14:43:40: 2000000 INFO @ Tue, 02 Aug 2022 14:43:45: 3000000 INFO @ Tue, 02 Aug 2022 14:43:50: 4000000 INFO @ Tue, 02 Aug 2022 14:43:55: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 14:43:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX15206427/SRX15206427.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX15206427/SRX15206427.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX15206427/SRX15206427.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX15206427/SRX15206427.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 14:43:58: #1 read tag files... INFO @ Tue, 02 Aug 2022 14:43:58: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 14:44:01: 6000000 INFO @ Tue, 02 Aug 2022 14:44:04: 1000000 INFO @ Tue, 02 Aug 2022 14:44:06: 7000000 INFO @ Tue, 02 Aug 2022 14:44:11: 2000000 INFO @ Tue, 02 Aug 2022 14:44:12: 8000000 INFO @ Tue, 02 Aug 2022 14:44:13: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 14:44:13: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 14:44:13: #1 total tags in treatment: 8235563 INFO @ Tue, 02 Aug 2022 14:44:13: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 14:44:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 14:44:13: #1 tags after filtering in treatment: 8235257 INFO @ Tue, 02 Aug 2022 14:44:13: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 14:44:13: #1 finished! INFO @ Tue, 02 Aug 2022 14:44:13: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 14:44:13: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 14:44:14: #2 number of paired peaks: 5037 INFO @ Tue, 02 Aug 2022 14:44:14: start model_add_line... INFO @ Tue, 02 Aug 2022 14:44:14: start X-correlation... INFO @ Tue, 02 Aug 2022 14:44:15: end of X-cor INFO @ Tue, 02 Aug 2022 14:44:15: #2 finished! INFO @ Tue, 02 Aug 2022 14:44:15: #2 predicted fragment length is 170 bps INFO @ Tue, 02 Aug 2022 14:44:15: #2 alternative fragment length(s) may be 170 bps INFO @ Tue, 02 Aug 2022 14:44:15: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX15206427/SRX15206427.05_model.r INFO @ Tue, 02 Aug 2022 14:44:15: #3 Call peaks... INFO @ Tue, 02 Aug 2022 14:44:15: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 14:44:17: 3000000 INFO @ Tue, 02 Aug 2022 14:44:23: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 14:44:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX15206427/SRX15206427.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX15206427/SRX15206427.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX15206427/SRX15206427.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX15206427/SRX15206427.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 14:44:28: #1 read tag files... INFO @ Tue, 02 Aug 2022 14:44:28: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 14:44:28: 5000000 INFO @ Tue, 02 Aug 2022 14:44:34: 1000000 INFO @ Tue, 02 Aug 2022 14:44:35: 6000000 INFO @ Tue, 02 Aug 2022 14:44:38: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 14:44:41: 2000000 INFO @ Tue, 02 Aug 2022 14:44:41: 7000000 INFO @ Tue, 02 Aug 2022 14:44:47: 3000000 INFO @ Tue, 02 Aug 2022 14:44:47: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX15206427/SRX15206427.05_peaks.xls INFO @ Tue, 02 Aug 2022 14:44:47: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX15206427/SRX15206427.05_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 14:44:47: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX15206427/SRX15206427.05_summits.bed INFO @ Tue, 02 Aug 2022 14:44:47: 8000000 INFO @ Tue, 02 Aug 2022 14:44:47: Done! pass1 - making usageList (484 chroms): 2 millis pass2 - checking and writing primary data (6659 records, 4 fields): 87 millis CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 14:44:49: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 14:44:49: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 14:44:49: #1 total tags in treatment: 8235563 INFO @ Tue, 02 Aug 2022 14:44:49: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 14:44:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 14:44:49: #1 tags after filtering in treatment: 8235257 INFO @ Tue, 02 Aug 2022 14:44:49: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 14:44:49: #1 finished! INFO @ Tue, 02 Aug 2022 14:44:49: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 14:44:49: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 14:44:50: #2 number of paired peaks: 5037 INFO @ Tue, 02 Aug 2022 14:44:50: start model_add_line... INFO @ Tue, 02 Aug 2022 14:44:50: start X-correlation... INFO @ Tue, 02 Aug 2022 14:44:50: end of X-cor INFO @ Tue, 02 Aug 2022 14:44:50: #2 finished! INFO @ Tue, 02 Aug 2022 14:44:50: #2 predicted fragment length is 170 bps INFO @ Tue, 02 Aug 2022 14:44:50: #2 alternative fragment length(s) may be 170 bps INFO @ Tue, 02 Aug 2022 14:44:50: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX15206427/SRX15206427.10_model.r INFO @ Tue, 02 Aug 2022 14:44:50: #3 Call peaks... INFO @ Tue, 02 Aug 2022 14:44:50: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 14:44:53: 4000000 INFO @ Tue, 02 Aug 2022 14:44:59: 5000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 14:45:05: 6000000 INFO @ Tue, 02 Aug 2022 14:45:12: 7000000 INFO @ Tue, 02 Aug 2022 14:45:15: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 14:45:18: 8000000 INFO @ Tue, 02 Aug 2022 14:45:20: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 14:45:20: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 14:45:20: #1 total tags in treatment: 8235563 INFO @ Tue, 02 Aug 2022 14:45:20: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 14:45:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 14:45:20: #1 tags after filtering in treatment: 8235257 INFO @ Tue, 02 Aug 2022 14:45:20: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 14:45:20: #1 finished! INFO @ Tue, 02 Aug 2022 14:45:20: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 14:45:20: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 14:45:21: #2 number of paired peaks: 5037 INFO @ Tue, 02 Aug 2022 14:45:21: start model_add_line... BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 14:45:21: start X-correlation... INFO @ Tue, 02 Aug 2022 14:45:21: end of X-cor INFO @ Tue, 02 Aug 2022 14:45:21: #2 finished! INFO @ Tue, 02 Aug 2022 14:45:21: #2 predicted fragment length is 170 bps INFO @ Tue, 02 Aug 2022 14:45:21: #2 alternative fragment length(s) may be 170 bps INFO @ Tue, 02 Aug 2022 14:45:21: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX15206427/SRX15206427.20_model.r INFO @ Tue, 02 Aug 2022 14:45:21: #3 Call peaks... INFO @ Tue, 02 Aug 2022 14:45:21: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 14:45:24: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX15206427/SRX15206427.10_peaks.xls INFO @ Tue, 02 Aug 2022 14:45:24: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX15206427/SRX15206427.10_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 14:45:24: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX15206427/SRX15206427.10_summits.bed INFO @ Tue, 02 Aug 2022 14:45:24: Done! pass1 - making usageList (421 chroms): 2 millis pass2 - checking and writing primary data (4188 records, 4 fields): 34 millis CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 14:45:45: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 14:45:55: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX15206427/SRX15206427.20_peaks.xls INFO @ Tue, 02 Aug 2022 14:45:55: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX15206427/SRX15206427.20_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 14:45:55: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX15206427/SRX15206427.20_summits.bed INFO @ Tue, 02 Aug 2022 14:45:55: Done! pass1 - making usageList (345 chroms): 1 millis pass2 - checking and writing primary data (2250 records, 4 fields): 30 millis CompletedMACS2peakCalling