Job ID = 16437462 SRX = SRX15046049 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:25 20311252 reads; of these: 20311252 (100.00%) were unpaired; of these: 10684940 (52.61%) aligned 0 times 7885317 (38.82%) aligned exactly 1 time 1740995 (8.57%) aligned >1 times 47.39% overall alignment rate Time searching: 00:03:25 Overall time: 00:03:25 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_rmdupse_core] 3384074 / 9626312 = 0.3515 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 12:40:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX15046049/SRX15046049.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX15046049/SRX15046049.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX15046049/SRX15046049.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX15046049/SRX15046049.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 12:40:30: #1 read tag files... INFO @ Tue, 02 Aug 2022 12:40:30: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 12:40:35: 1000000 INFO @ Tue, 02 Aug 2022 12:40:40: 2000000 INFO @ Tue, 02 Aug 2022 12:40:45: 3000000 INFO @ Tue, 02 Aug 2022 12:40:50: 4000000 INFO @ Tue, 02 Aug 2022 12:40:56: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 12:40:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX15046049/SRX15046049.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX15046049/SRX15046049.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX15046049/SRX15046049.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX15046049/SRX15046049.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 12:40:59: #1 read tag files... INFO @ Tue, 02 Aug 2022 12:40:59: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 12:41:01: 6000000 INFO @ Tue, 02 Aug 2022 12:41:03: #1 tag size is determined as 51 bps INFO @ Tue, 02 Aug 2022 12:41:03: #1 tag size = 51 INFO @ Tue, 02 Aug 2022 12:41:03: #1 total tags in treatment: 6242238 INFO @ Tue, 02 Aug 2022 12:41:03: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 12:41:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 12:41:03: #1 tags after filtering in treatment: 6242219 INFO @ Tue, 02 Aug 2022 12:41:03: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 12:41:03: #1 finished! INFO @ Tue, 02 Aug 2022 12:41:03: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 12:41:03: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 12:41:03: #2 number of paired peaks: 1365 INFO @ Tue, 02 Aug 2022 12:41:03: start model_add_line... INFO @ Tue, 02 Aug 2022 12:41:04: start X-correlation... INFO @ Tue, 02 Aug 2022 12:41:04: end of X-cor INFO @ Tue, 02 Aug 2022 12:41:04: #2 finished! INFO @ Tue, 02 Aug 2022 12:41:04: #2 predicted fragment length is 141 bps INFO @ Tue, 02 Aug 2022 12:41:04: #2 alternative fragment length(s) may be 141 bps INFO @ Tue, 02 Aug 2022 12:41:04: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX15046049/SRX15046049.05_model.r INFO @ Tue, 02 Aug 2022 12:41:04: #3 Call peaks... INFO @ Tue, 02 Aug 2022 12:41:04: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 12:41:05: 1000000 INFO @ Tue, 02 Aug 2022 12:41:11: 2000000 INFO @ Tue, 02 Aug 2022 12:41:16: 3000000 INFO @ Tue, 02 Aug 2022 12:41:18: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 12:41:21: 4000000 INFO @ Tue, 02 Aug 2022 12:41:26: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX15046049/SRX15046049.05_peaks.xls INFO @ Tue, 02 Aug 2022 12:41:26: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX15046049/SRX15046049.05_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 12:41:26: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX15046049/SRX15046049.05_summits.bed INFO @ Tue, 02 Aug 2022 12:41:26: Done! pass1 - making usageList (424 chroms): 1 millis pass2 - checking and writing primary data (1815 records, 4 fields): 43 millis CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 12:41:27: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 12:41:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX15046049/SRX15046049.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX15046049/SRX15046049.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX15046049/SRX15046049.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX15046049/SRX15046049.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 12:41:29: #1 read tag files... INFO @ Tue, 02 Aug 2022 12:41:29: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 12:41:32: 6000000 INFO @ Tue, 02 Aug 2022 12:41:34: #1 tag size is determined as 51 bps INFO @ Tue, 02 Aug 2022 12:41:34: #1 tag size = 51 INFO @ Tue, 02 Aug 2022 12:41:34: #1 total tags in treatment: 6242238 INFO @ Tue, 02 Aug 2022 12:41:34: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 12:41:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 12:41:34: #1 tags after filtering in treatment: 6242219 INFO @ Tue, 02 Aug 2022 12:41:34: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 12:41:34: #1 finished! INFO @ Tue, 02 Aug 2022 12:41:34: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 12:41:34: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 12:41:35: #2 number of paired peaks: 1365 INFO @ Tue, 02 Aug 2022 12:41:35: start model_add_line... INFO @ Tue, 02 Aug 2022 12:41:35: start X-correlation... INFO @ Tue, 02 Aug 2022 12:41:35: end of X-cor INFO @ Tue, 02 Aug 2022 12:41:35: #2 finished! INFO @ Tue, 02 Aug 2022 12:41:35: #2 predicted fragment length is 141 bps INFO @ Tue, 02 Aug 2022 12:41:35: #2 alternative fragment length(s) may be 141 bps INFO @ Tue, 02 Aug 2022 12:41:35: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX15046049/SRX15046049.10_model.r INFO @ Tue, 02 Aug 2022 12:41:35: #3 Call peaks... INFO @ Tue, 02 Aug 2022 12:41:35: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 12:41:35: 1000000 INFO @ Tue, 02 Aug 2022 12:41:40: 2000000 INFO @ Tue, 02 Aug 2022 12:41:45: 3000000 INFO @ Tue, 02 Aug 2022 12:41:50: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 12:41:50: 4000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 12:41:56: 5000000 INFO @ Tue, 02 Aug 2022 12:41:58: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX15046049/SRX15046049.10_peaks.xls INFO @ Tue, 02 Aug 2022 12:41:58: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX15046049/SRX15046049.10_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 12:41:58: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX15046049/SRX15046049.10_summits.bed INFO @ Tue, 02 Aug 2022 12:41:58: Done! pass1 - making usageList (311 chroms): 1 millis pass2 - checking and writing primary data (1066 records, 4 fields): 41 millis CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 12:42:01: 6000000 INFO @ Tue, 02 Aug 2022 12:42:02: #1 tag size is determined as 51 bps INFO @ Tue, 02 Aug 2022 12:42:02: #1 tag size = 51 INFO @ Tue, 02 Aug 2022 12:42:02: #1 total tags in treatment: 6242238 INFO @ Tue, 02 Aug 2022 12:42:02: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 12:42:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 12:42:03: #1 tags after filtering in treatment: 6242219 INFO @ Tue, 02 Aug 2022 12:42:03: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 12:42:03: #1 finished! INFO @ Tue, 02 Aug 2022 12:42:03: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 12:42:03: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 12:42:03: #2 number of paired peaks: 1365 INFO @ Tue, 02 Aug 2022 12:42:03: start model_add_line... INFO @ Tue, 02 Aug 2022 12:42:03: start X-correlation... INFO @ Tue, 02 Aug 2022 12:42:03: end of X-cor INFO @ Tue, 02 Aug 2022 12:42:03: #2 finished! INFO @ Tue, 02 Aug 2022 12:42:03: #2 predicted fragment length is 141 bps INFO @ Tue, 02 Aug 2022 12:42:03: #2 alternative fragment length(s) may be 141 bps INFO @ Tue, 02 Aug 2022 12:42:03: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX15046049/SRX15046049.20_model.r INFO @ Tue, 02 Aug 2022 12:42:04: #3 Call peaks... INFO @ Tue, 02 Aug 2022 12:42:04: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 12:42:19: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 12:42:26: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX15046049/SRX15046049.20_peaks.xls INFO @ Tue, 02 Aug 2022 12:42:26: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX15046049/SRX15046049.20_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 12:42:26: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX15046049/SRX15046049.20_summits.bed INFO @ Tue, 02 Aug 2022 12:42:27: Done! pass1 - making usageList (137 chroms): 1 millis pass2 - checking and writing primary data (521 records, 4 fields): 32 millis CompletedMACS2peakCalling