Job ID = 16439863 SRX = SRX14894242 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:34 45229310 reads; of these: 45229310 (100.00%) were unpaired; of these: 15969282 (35.31%) aligned 0 times 24119198 (53.33%) aligned exactly 1 time 5140830 (11.37%) aligned >1 times 64.69% overall alignment rate Time searching: 00:11:34 Overall time: 00:11:34 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 14890822 / 29260028 = 0.5089 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 16:44:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX14894242/SRX14894242.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX14894242/SRX14894242.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX14894242/SRX14894242.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX14894242/SRX14894242.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 16:44:12: #1 read tag files... INFO @ Tue, 02 Aug 2022 16:44:12: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 16:44:23: 1000000 INFO @ Tue, 02 Aug 2022 16:44:33: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 16:44:42: 3000000 INFO @ Tue, 02 Aug 2022 16:44:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX14894242/SRX14894242.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX14894242/SRX14894242.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX14894242/SRX14894242.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX14894242/SRX14894242.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 16:44:42: #1 read tag files... INFO @ Tue, 02 Aug 2022 16:44:42: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 16:44:52: 4000000 INFO @ Tue, 02 Aug 2022 16:44:54: 1000000 INFO @ Tue, 02 Aug 2022 16:45:01: 5000000 INFO @ Tue, 02 Aug 2022 16:45:06: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 16:45:12: 6000000 INFO @ Tue, 02 Aug 2022 16:45:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX14894242/SRX14894242.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX14894242/SRX14894242.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX14894242/SRX14894242.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX14894242/SRX14894242.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 16:45:12: #1 read tag files... INFO @ Tue, 02 Aug 2022 16:45:12: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 16:45:18: 3000000 INFO @ Tue, 02 Aug 2022 16:45:23: 7000000 INFO @ Tue, 02 Aug 2022 16:45:25: 1000000 INFO @ Tue, 02 Aug 2022 16:45:29: 4000000 INFO @ Tue, 02 Aug 2022 16:45:33: 8000000 INFO @ Tue, 02 Aug 2022 16:45:37: 2000000 INFO @ Tue, 02 Aug 2022 16:45:41: 5000000 INFO @ Tue, 02 Aug 2022 16:45:43: 9000000 INFO @ Tue, 02 Aug 2022 16:45:50: 3000000 INFO @ Tue, 02 Aug 2022 16:45:54: 6000000 INFO @ Tue, 02 Aug 2022 16:45:54: 10000000 INFO @ Tue, 02 Aug 2022 16:46:03: 4000000 INFO @ Tue, 02 Aug 2022 16:46:04: 11000000 INFO @ Tue, 02 Aug 2022 16:46:06: 7000000 INFO @ Tue, 02 Aug 2022 16:46:14: 5000000 INFO @ Tue, 02 Aug 2022 16:46:15: 12000000 INFO @ Tue, 02 Aug 2022 16:46:17: 8000000 INFO @ Tue, 02 Aug 2022 16:46:25: 13000000 INFO @ Tue, 02 Aug 2022 16:46:27: 6000000 INFO @ Tue, 02 Aug 2022 16:46:29: 9000000 INFO @ Tue, 02 Aug 2022 16:46:35: 14000000 INFO @ Tue, 02 Aug 2022 16:46:39: 7000000 INFO @ Tue, 02 Aug 2022 16:46:39: #1 tag size is determined as 51 bps INFO @ Tue, 02 Aug 2022 16:46:39: #1 tag size = 51 INFO @ Tue, 02 Aug 2022 16:46:39: #1 total tags in treatment: 14369206 INFO @ Tue, 02 Aug 2022 16:46:39: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 16:46:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 16:46:40: #1 tags after filtering in treatment: 14369115 INFO @ Tue, 02 Aug 2022 16:46:40: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 16:46:40: #1 finished! INFO @ Tue, 02 Aug 2022 16:46:40: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 16:46:40: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 16:46:40: 10000000 INFO @ Tue, 02 Aug 2022 16:46:42: #2 number of paired peaks: 3996 INFO @ Tue, 02 Aug 2022 16:46:42: start model_add_line... INFO @ Tue, 02 Aug 2022 16:46:42: start X-correlation... INFO @ Tue, 02 Aug 2022 16:46:42: end of X-cor INFO @ Tue, 02 Aug 2022 16:46:42: #2 finished! INFO @ Tue, 02 Aug 2022 16:46:42: #2 predicted fragment length is 247 bps INFO @ Tue, 02 Aug 2022 16:46:42: #2 alternative fragment length(s) may be 247 bps INFO @ Tue, 02 Aug 2022 16:46:42: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX14894242/SRX14894242.05_model.r INFO @ Tue, 02 Aug 2022 16:46:42: #3 Call peaks... INFO @ Tue, 02 Aug 2022 16:46:42: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 16:46:51: 8000000 INFO @ Tue, 02 Aug 2022 16:46:52: 11000000 INFO @ Tue, 02 Aug 2022 16:47:03: 9000000 INFO @ Tue, 02 Aug 2022 16:47:05: 12000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 16:47:16: 10000000 INFO @ Tue, 02 Aug 2022 16:47:17: 13000000 INFO @ Tue, 02 Aug 2022 16:47:27: 11000000 INFO @ Tue, 02 Aug 2022 16:47:29: 14000000 INFO @ Tue, 02 Aug 2022 16:47:33: #1 tag size is determined as 51 bps INFO @ Tue, 02 Aug 2022 16:47:33: #1 tag size = 51 INFO @ Tue, 02 Aug 2022 16:47:33: #1 total tags in treatment: 14369206 INFO @ Tue, 02 Aug 2022 16:47:33: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 16:47:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 16:47:34: #1 tags after filtering in treatment: 14369115 INFO @ Tue, 02 Aug 2022 16:47:34: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 16:47:34: #1 finished! INFO @ Tue, 02 Aug 2022 16:47:34: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 16:47:34: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 16:47:36: #2 number of paired peaks: 3996 INFO @ Tue, 02 Aug 2022 16:47:36: start model_add_line... INFO @ Tue, 02 Aug 2022 16:47:36: start X-correlation... INFO @ Tue, 02 Aug 2022 16:47:36: end of X-cor INFO @ Tue, 02 Aug 2022 16:47:36: #2 finished! INFO @ Tue, 02 Aug 2022 16:47:36: #2 predicted fragment length is 247 bps INFO @ Tue, 02 Aug 2022 16:47:36: #2 alternative fragment length(s) may be 247 bps INFO @ Tue, 02 Aug 2022 16:47:36: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX14894242/SRX14894242.10_model.r INFO @ Tue, 02 Aug 2022 16:47:36: #3 Call peaks... INFO @ Tue, 02 Aug 2022 16:47:36: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 16:47:38: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 16:47:39: 12000000 INFO @ Tue, 02 Aug 2022 16:47:51: 13000000 BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 16:48:02: 14000000 INFO @ Tue, 02 Aug 2022 16:48:04: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX14894242/SRX14894242.05_peaks.xls INFO @ Tue, 02 Aug 2022 16:48:04: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX14894242/SRX14894242.05_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 16:48:04: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX14894242/SRX14894242.05_summits.bed INFO @ Tue, 02 Aug 2022 16:48:04: Done! pass1 - making usageList (636 chroms): 5 millis pass2 - checking and writing primary data (10565 records, 4 fields): 64 millis CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 16:48:07: #1 tag size is determined as 51 bps INFO @ Tue, 02 Aug 2022 16:48:07: #1 tag size = 51 INFO @ Tue, 02 Aug 2022 16:48:07: #1 total tags in treatment: 14369206 INFO @ Tue, 02 Aug 2022 16:48:07: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 16:48:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 16:48:07: #1 tags after filtering in treatment: 14369115 INFO @ Tue, 02 Aug 2022 16:48:07: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 16:48:07: #1 finished! INFO @ Tue, 02 Aug 2022 16:48:07: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 16:48:07: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 16:48:09: #2 number of paired peaks: 3996 INFO @ Tue, 02 Aug 2022 16:48:09: start model_add_line... INFO @ Tue, 02 Aug 2022 16:48:10: start X-correlation... INFO @ Tue, 02 Aug 2022 16:48:10: end of X-cor INFO @ Tue, 02 Aug 2022 16:48:10: #2 finished! INFO @ Tue, 02 Aug 2022 16:48:10: #2 predicted fragment length is 247 bps INFO @ Tue, 02 Aug 2022 16:48:10: #2 alternative fragment length(s) may be 247 bps INFO @ Tue, 02 Aug 2022 16:48:10: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm6/SRX14894242/SRX14894242.20_model.r INFO @ Tue, 02 Aug 2022 16:48:10: #3 Call peaks... INFO @ Tue, 02 Aug 2022 16:48:10: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 16:48:35: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 16:49:01: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX14894242/SRX14894242.10_peaks.xls INFO @ Tue, 02 Aug 2022 16:49:01: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX14894242/SRX14894242.10_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 16:49:01: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX14894242/SRX14894242.10_summits.bed INFO @ Tue, 02 Aug 2022 16:49:01: Done! pass1 - making usageList (479 chroms): 4 millis pass2 - checking and writing primary data (8572 records, 4 fields): 95 millis CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 16:49:07: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 16:49:32: #4 Write output xls file... /home/okishinya/chipatlas/results/dm6/SRX14894242/SRX14894242.20_peaks.xls INFO @ Tue, 02 Aug 2022 16:49:33: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm6/SRX14894242/SRX14894242.20_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 16:49:33: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm6/SRX14894242/SRX14894242.20_summits.bed INFO @ Tue, 02 Aug 2022 16:49:33: Done! pass1 - making usageList (285 chroms): 2 millis pass2 - checking and writing primary data (6536 records, 4 fields): 61 millis CompletedMACS2peakCalling