Job ID = 16440058 SRX = SRX14894239 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:16:40 62620679 reads; of these: 62620679 (100.00%) were unpaired; of these: 10167325 (16.24%) aligned 0 times 38179594 (60.97%) aligned exactly 1 time 14273760 (22.79%) aligned >1 times 83.76% overall alignment rate Time searching: 00:16:41 Overall time: 00:16:41 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 15048640 / 52453354 = 0.2869 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 17:17:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX14894239/SRX14894239.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX14894239/SRX14894239.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX14894239/SRX14894239.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX14894239/SRX14894239.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 17:17:39: #1 read tag files... INFO @ Tue, 02 Aug 2022 17:17:39: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 17:17:44: 1000000 INFO @ Tue, 02 Aug 2022 17:17:50: 2000000 INFO @ Tue, 02 Aug 2022 17:17:55: 3000000 INFO @ Tue, 02 Aug 2022 17:18:00: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 17:18:06: 5000000 INFO @ Tue, 02 Aug 2022 17:18:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX14894239/SRX14894239.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX14894239/SRX14894239.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX14894239/SRX14894239.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX14894239/SRX14894239.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 17:18:08: #1 read tag files... INFO @ Tue, 02 Aug 2022 17:18:08: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 17:18:11: 6000000 INFO @ Tue, 02 Aug 2022 17:18:14: 1000000 INFO @ Tue, 02 Aug 2022 17:18:16: 7000000 INFO @ Tue, 02 Aug 2022 17:18:21: 2000000 INFO @ Tue, 02 Aug 2022 17:18:22: 8000000 INFO @ Tue, 02 Aug 2022 17:18:27: 9000000 INFO @ Tue, 02 Aug 2022 17:18:27: 3000000 INFO @ Tue, 02 Aug 2022 17:18:32: 10000000 INFO @ Tue, 02 Aug 2022 17:18:34: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 17:18:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX14894239/SRX14894239.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX14894239/SRX14894239.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX14894239/SRX14894239.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX14894239/SRX14894239.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 17:18:38: #1 read tag files... INFO @ Tue, 02 Aug 2022 17:18:38: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 17:18:39: 11000000 INFO @ Tue, 02 Aug 2022 17:18:41: 5000000 INFO @ Tue, 02 Aug 2022 17:18:44: 1000000 INFO @ Tue, 02 Aug 2022 17:18:44: 12000000 INFO @ Tue, 02 Aug 2022 17:18:47: 6000000 INFO @ Tue, 02 Aug 2022 17:18:50: 2000000 INFO @ Tue, 02 Aug 2022 17:18:50: 13000000 INFO @ Tue, 02 Aug 2022 17:18:54: 7000000 INFO @ Tue, 02 Aug 2022 17:18:56: 3000000 INFO @ Tue, 02 Aug 2022 17:18:57: 14000000 INFO @ Tue, 02 Aug 2022 17:19:01: 8000000 INFO @ Tue, 02 Aug 2022 17:19:03: 15000000 INFO @ Tue, 02 Aug 2022 17:19:03: 4000000 INFO @ Tue, 02 Aug 2022 17:19:07: 9000000 INFO @ Tue, 02 Aug 2022 17:19:09: 16000000 INFO @ Tue, 02 Aug 2022 17:19:09: 5000000 INFO @ Tue, 02 Aug 2022 17:19:14: 10000000 INFO @ Tue, 02 Aug 2022 17:19:15: 17000000 INFO @ Tue, 02 Aug 2022 17:19:15: 6000000 INFO @ Tue, 02 Aug 2022 17:19:21: 18000000 INFO @ Tue, 02 Aug 2022 17:19:21: 7000000 INFO @ Tue, 02 Aug 2022 17:19:21: 11000000 INFO @ Tue, 02 Aug 2022 17:19:27: 19000000 INFO @ Tue, 02 Aug 2022 17:19:27: 8000000 INFO @ Tue, 02 Aug 2022 17:19:28: 12000000 INFO @ Tue, 02 Aug 2022 17:19:32: 20000000 INFO @ Tue, 02 Aug 2022 17:19:33: 9000000 INFO @ Tue, 02 Aug 2022 17:19:34: 13000000 INFO @ Tue, 02 Aug 2022 17:19:38: 21000000 INFO @ Tue, 02 Aug 2022 17:19:39: 10000000 INFO @ Tue, 02 Aug 2022 17:19:42: 14000000 INFO @ Tue, 02 Aug 2022 17:19:45: 22000000 INFO @ Tue, 02 Aug 2022 17:19:45: 11000000 INFO @ Tue, 02 Aug 2022 17:19:48: 15000000 INFO @ Tue, 02 Aug 2022 17:19:50: 23000000 INFO @ Tue, 02 Aug 2022 17:19:51: 12000000 INFO @ Tue, 02 Aug 2022 17:19:55: 16000000 INFO @ Tue, 02 Aug 2022 17:19:56: 24000000 INFO @ Tue, 02 Aug 2022 17:19:57: 13000000 INFO @ Tue, 02 Aug 2022 17:20:02: 17000000 INFO @ Tue, 02 Aug 2022 17:20:03: 25000000 INFO @ Tue, 02 Aug 2022 17:20:03: 14000000 INFO @ Tue, 02 Aug 2022 17:20:09: 26000000 INFO @ Tue, 02 Aug 2022 17:20:09: 18000000 INFO @ Tue, 02 Aug 2022 17:20:09: 15000000 INFO @ Tue, 02 Aug 2022 17:20:15: 27000000 INFO @ Tue, 02 Aug 2022 17:20:15: 16000000 INFO @ Tue, 02 Aug 2022 17:20:16: 19000000 INFO @ Tue, 02 Aug 2022 17:20:21: 28000000 INFO @ Tue, 02 Aug 2022 17:20:21: 17000000 INFO @ Tue, 02 Aug 2022 17:20:23: 20000000 INFO @ Tue, 02 Aug 2022 17:20:27: 29000000 INFO @ Tue, 02 Aug 2022 17:20:27: 18000000 INFO @ Tue, 02 Aug 2022 17:20:30: 21000000 INFO @ Tue, 02 Aug 2022 17:20:33: 19000000 INFO @ Tue, 02 Aug 2022 17:20:33: 30000000 INFO @ Tue, 02 Aug 2022 17:20:36: 22000000 INFO @ Tue, 02 Aug 2022 17:20:39: 20000000 INFO @ Tue, 02 Aug 2022 17:20:39: 31000000 INFO @ Tue, 02 Aug 2022 17:20:42: 23000000 INFO @ Tue, 02 Aug 2022 17:20:45: 32000000 INFO @ Tue, 02 Aug 2022 17:20:47: 21000000 INFO @ Tue, 02 Aug 2022 17:20:49: 24000000 INFO @ Tue, 02 Aug 2022 17:20:52: 33000000 INFO @ Tue, 02 Aug 2022 17:20:53: 22000000 INFO @ Tue, 02 Aug 2022 17:20:56: 25000000 INFO @ Tue, 02 Aug 2022 17:20:58: 34000000 INFO @ Tue, 02 Aug 2022 17:20:59: 23000000 INFO @ Tue, 02 Aug 2022 17:21:03: 26000000 INFO @ Tue, 02 Aug 2022 17:21:04: 35000000 INFO @ Tue, 02 Aug 2022 17:21:04: 24000000 INFO @ Tue, 02 Aug 2022 17:21:09: 27000000 INFO @ Tue, 02 Aug 2022 17:21:10: 36000000 INFO @ Tue, 02 Aug 2022 17:21:10: 25000000 INFO @ Tue, 02 Aug 2022 17:21:16: 28000000 INFO @ Tue, 02 Aug 2022 17:21:16: 26000000 INFO @ Tue, 02 Aug 2022 17:21:16: 37000000 INFO @ Tue, 02 Aug 2022 17:21:19: #1 tag size is determined as 51 bps INFO @ Tue, 02 Aug 2022 17:21:19: #1 tag size = 51 INFO @ Tue, 02 Aug 2022 17:21:19: #1 total tags in treatment: 37404714 INFO @ Tue, 02 Aug 2022 17:21:19: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 17:21:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 17:21:20: #1 tags after filtering in treatment: 37404678 INFO @ Tue, 02 Aug 2022 17:21:20: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 17:21:20: #1 finished! INFO @ Tue, 02 Aug 2022 17:21:20: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 17:21:20: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 17:21:22: 27000000 INFO @ Tue, 02 Aug 2022 17:21:23: 29000000 INFO @ Tue, 02 Aug 2022 17:21:23: #2 number of paired peaks: 53 WARNING @ Tue, 02 Aug 2022 17:21:23: Too few paired peaks (53) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 17:21:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX14894239/SRX14894239.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 11 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX14894239/SRX14894239.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX14894239/SRX14894239.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX14894239/SRX14894239.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 17:21:27: 28000000 INFO @ Tue, 02 Aug 2022 17:21:30: 30000000 INFO @ Tue, 02 Aug 2022 17:21:33: 29000000 INFO @ Tue, 02 Aug 2022 17:21:37: 31000000 INFO @ Tue, 02 Aug 2022 17:21:39: 30000000 INFO @ Tue, 02 Aug 2022 17:21:44: 32000000 INFO @ Tue, 02 Aug 2022 17:21:45: 31000000 INFO @ Tue, 02 Aug 2022 17:21:50: 32000000 INFO @ Tue, 02 Aug 2022 17:21:51: 33000000 INFO @ Tue, 02 Aug 2022 17:21:55: 33000000 INFO @ Tue, 02 Aug 2022 17:21:57: 34000000 INFO @ Tue, 02 Aug 2022 17:22:00: 34000000 INFO @ Tue, 02 Aug 2022 17:22:04: 35000000 INFO @ Tue, 02 Aug 2022 17:22:06: 35000000 INFO @ Tue, 02 Aug 2022 17:22:11: 36000000 INFO @ Tue, 02 Aug 2022 17:22:12: 36000000 INFO @ Tue, 02 Aug 2022 17:22:18: 37000000 INFO @ Tue, 02 Aug 2022 17:22:18: 37000000 INFO @ Tue, 02 Aug 2022 17:22:21: #1 tag size is determined as 51 bps INFO @ Tue, 02 Aug 2022 17:22:21: #1 tag size = 51 INFO @ Tue, 02 Aug 2022 17:22:21: #1 total tags in treatment: 37404714 INFO @ Tue, 02 Aug 2022 17:22:21: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 17:22:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 17:22:21: #1 tag size is determined as 51 bps INFO @ Tue, 02 Aug 2022 17:22:21: #1 tag size = 51 INFO @ Tue, 02 Aug 2022 17:22:21: #1 total tags in treatment: 37404714 INFO @ Tue, 02 Aug 2022 17:22:21: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 17:22:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 17:22:22: #1 tags after filtering in treatment: 37404678 INFO @ Tue, 02 Aug 2022 17:22:22: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 17:22:22: #1 finished! INFO @ Tue, 02 Aug 2022 17:22:22: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 17:22:22: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 17:22:23: #1 tags after filtering in treatment: 37404678 INFO @ Tue, 02 Aug 2022 17:22:23: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 17:22:23: #1 finished! INFO @ Tue, 02 Aug 2022 17:22:23: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 17:22:23: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 17:22:24: #2 number of paired peaks: 53 WARNING @ Tue, 02 Aug 2022 17:22:24: Too few paired peaks (53) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 17:22:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX14894239/SRX14894239.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX14894239/SRX14894239.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX14894239/SRX14894239.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX14894239/SRX14894239.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 17:22:25: #2 number of paired peaks: 53 WARNING @ Tue, 02 Aug 2022 17:22:25: Too few paired peaks (53) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 17:22:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX14894239/SRX14894239.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX14894239/SRX14894239.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX14894239/SRX14894239.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX14894239/SRX14894239.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。