Job ID = 16440025 SRX = SRX14894238 Genome = dm6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:17:54 68719034 reads; of these: 68719034 (100.00%) were unpaired; of these: 6446605 (9.38%) aligned 0 times 45831993 (66.69%) aligned exactly 1 time 16440436 (23.92%) aligned >1 times 90.62% overall alignment rate Time searching: 00:17:54 Overall time: 00:17:54 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 1870 sequences. [bam_sort_core] merging from 28 files... [bam_rmdupse_core] 19517880 / 62272429 = 0.3134 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 17:12:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX14894238/SRX14894238.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX14894238/SRX14894238.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX14894238/SRX14894238.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX14894238/SRX14894238.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 17:12:40: #1 read tag files... INFO @ Tue, 02 Aug 2022 17:12:40: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 17:12:47: 1000000 INFO @ Tue, 02 Aug 2022 17:12:53: 2000000 INFO @ Tue, 02 Aug 2022 17:12:59: 3000000 INFO @ Tue, 02 Aug 2022 17:13:05: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 17:13:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX14894238/SRX14894238.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX14894238/SRX14894238.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX14894238/SRX14894238.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX14894238/SRX14894238.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 17:13:09: #1 read tag files... INFO @ Tue, 02 Aug 2022 17:13:09: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 17:13:11: 5000000 INFO @ Tue, 02 Aug 2022 17:13:16: 1000000 INFO @ Tue, 02 Aug 2022 17:13:17: 6000000 INFO @ Tue, 02 Aug 2022 17:13:24: 2000000 INFO @ Tue, 02 Aug 2022 17:13:24: 7000000 INFO @ Tue, 02 Aug 2022 17:13:30: 8000000 INFO @ Tue, 02 Aug 2022 17:13:31: 3000000 INFO @ Tue, 02 Aug 2022 17:13:37: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 17:13:38: 4000000 INFO @ Tue, 02 Aug 2022 17:13:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm6/SRX14894238/SRX14894238.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm6/SRX14894238/SRX14894238.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm6/SRX14894238/SRX14894238.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm6/SRX14894238/SRX14894238.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 17:13:39: #1 read tag files... INFO @ Tue, 02 Aug 2022 17:13:39: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 17:13:43: 10000000 INFO @ Tue, 02 Aug 2022 17:13:45: 5000000 INFO @ Tue, 02 Aug 2022 17:13:46: 1000000 INFO @ Tue, 02 Aug 2022 17:13:50: 11000000 INFO @ Tue, 02 Aug 2022 17:13:52: 2000000 INFO @ Tue, 02 Aug 2022 17:13:52: 6000000 INFO @ Tue, 02 Aug 2022 17:13:57: 12000000 INFO @ Tue, 02 Aug 2022 17:13:58: 3000000 INFO @ Tue, 02 Aug 2022 17:13:59: 7000000 INFO @ Tue, 02 Aug 2022 17:14:04: 13000000 INFO @ Tue, 02 Aug 2022 17:14:04: 4000000 INFO @ Tue, 02 Aug 2022 17:14:07: 8000000 INFO @ Tue, 02 Aug 2022 17:14:10: 5000000 INFO @ Tue, 02 Aug 2022 17:14:11: 14000000 INFO @ Tue, 02 Aug 2022 17:14:14: 9000000 INFO @ Tue, 02 Aug 2022 17:14:16: 6000000 INFO @ Tue, 02 Aug 2022 17:14:17: 15000000 INFO @ Tue, 02 Aug 2022 17:14:21: 10000000 INFO @ Tue, 02 Aug 2022 17:14:22: 7000000 INFO @ Tue, 02 Aug 2022 17:14:24: 16000000 INFO @ Tue, 02 Aug 2022 17:14:28: 11000000 INFO @ Tue, 02 Aug 2022 17:14:28: 8000000 INFO @ Tue, 02 Aug 2022 17:14:31: 17000000 INFO @ Tue, 02 Aug 2022 17:14:34: 9000000 INFO @ Tue, 02 Aug 2022 17:14:35: 12000000 INFO @ Tue, 02 Aug 2022 17:14:38: 18000000 INFO @ Tue, 02 Aug 2022 17:14:41: 10000000 INFO @ Tue, 02 Aug 2022 17:14:42: 13000000 INFO @ Tue, 02 Aug 2022 17:14:45: 19000000 INFO @ Tue, 02 Aug 2022 17:14:47: 11000000 INFO @ Tue, 02 Aug 2022 17:14:49: 14000000 INFO @ Tue, 02 Aug 2022 17:14:52: 20000000 INFO @ Tue, 02 Aug 2022 17:14:53: 12000000 INFO @ Tue, 02 Aug 2022 17:14:56: 15000000 INFO @ Tue, 02 Aug 2022 17:14:59: 13000000 INFO @ Tue, 02 Aug 2022 17:14:59: 21000000 INFO @ Tue, 02 Aug 2022 17:15:03: 16000000 INFO @ Tue, 02 Aug 2022 17:15:05: 14000000 INFO @ Tue, 02 Aug 2022 17:15:06: 22000000 INFO @ Tue, 02 Aug 2022 17:15:10: 17000000 INFO @ Tue, 02 Aug 2022 17:15:10: 15000000 INFO @ Tue, 02 Aug 2022 17:15:13: 23000000 INFO @ Tue, 02 Aug 2022 17:15:16: 16000000 INFO @ Tue, 02 Aug 2022 17:15:17: 18000000 INFO @ Tue, 02 Aug 2022 17:15:20: 24000000 INFO @ Tue, 02 Aug 2022 17:15:22: 17000000 INFO @ Tue, 02 Aug 2022 17:15:24: 19000000 INFO @ Tue, 02 Aug 2022 17:15:26: 25000000 INFO @ Tue, 02 Aug 2022 17:15:28: 18000000 INFO @ Tue, 02 Aug 2022 17:15:32: 20000000 INFO @ Tue, 02 Aug 2022 17:15:33: 26000000 INFO @ Tue, 02 Aug 2022 17:15:34: 19000000 INFO @ Tue, 02 Aug 2022 17:15:39: 21000000 INFO @ Tue, 02 Aug 2022 17:15:40: 27000000 INFO @ Tue, 02 Aug 2022 17:15:40: 20000000 INFO @ Tue, 02 Aug 2022 17:15:46: 22000000 INFO @ Tue, 02 Aug 2022 17:15:47: 21000000 INFO @ Tue, 02 Aug 2022 17:15:47: 28000000 INFO @ Tue, 02 Aug 2022 17:15:53: 22000000 INFO @ Tue, 02 Aug 2022 17:15:53: 23000000 INFO @ Tue, 02 Aug 2022 17:15:54: 29000000 INFO @ Tue, 02 Aug 2022 17:15:59: 23000000 INFO @ Tue, 02 Aug 2022 17:16:00: 24000000 INFO @ Tue, 02 Aug 2022 17:16:01: 30000000 INFO @ Tue, 02 Aug 2022 17:16:05: 24000000 INFO @ Tue, 02 Aug 2022 17:16:07: 25000000 INFO @ Tue, 02 Aug 2022 17:16:07: 31000000 INFO @ Tue, 02 Aug 2022 17:16:11: 25000000 INFO @ Tue, 02 Aug 2022 17:16:14: 26000000 INFO @ Tue, 02 Aug 2022 17:16:14: 32000000 INFO @ Tue, 02 Aug 2022 17:16:17: 26000000 INFO @ Tue, 02 Aug 2022 17:16:20: 33000000 INFO @ Tue, 02 Aug 2022 17:16:21: 27000000 INFO @ Tue, 02 Aug 2022 17:16:23: 27000000 INFO @ Tue, 02 Aug 2022 17:16:27: 28000000 INFO @ Tue, 02 Aug 2022 17:16:28: 34000000 INFO @ Tue, 02 Aug 2022 17:16:29: 28000000 INFO @ Tue, 02 Aug 2022 17:16:34: 29000000 INFO @ Tue, 02 Aug 2022 17:16:35: 35000000 INFO @ Tue, 02 Aug 2022 17:16:36: 29000000 INFO @ Tue, 02 Aug 2022 17:16:41: 30000000 INFO @ Tue, 02 Aug 2022 17:16:42: 30000000 INFO @ Tue, 02 Aug 2022 17:16:42: 36000000 INFO @ Tue, 02 Aug 2022 17:16:48: 31000000 INFO @ Tue, 02 Aug 2022 17:16:49: 37000000 INFO @ Tue, 02 Aug 2022 17:16:49: 31000000 INFO @ Tue, 02 Aug 2022 17:16:55: 38000000 INFO @ Tue, 02 Aug 2022 17:16:55: 32000000 INFO @ Tue, 02 Aug 2022 17:16:56: 32000000 INFO @ Tue, 02 Aug 2022 17:17:01: 33000000 INFO @ Tue, 02 Aug 2022 17:17:02: 39000000 INFO @ Tue, 02 Aug 2022 17:17:03: 33000000 INFO @ Tue, 02 Aug 2022 17:17:08: 34000000 INFO @ Tue, 02 Aug 2022 17:17:09: 40000000 INFO @ Tue, 02 Aug 2022 17:17:10: 34000000 INFO @ Tue, 02 Aug 2022 17:17:14: 35000000 INFO @ Tue, 02 Aug 2022 17:17:15: 41000000 INFO @ Tue, 02 Aug 2022 17:17:18: 35000000 INFO @ Tue, 02 Aug 2022 17:17:20: 36000000 INFO @ Tue, 02 Aug 2022 17:17:23: 42000000 INFO @ Tue, 02 Aug 2022 17:17:25: 36000000 INFO @ Tue, 02 Aug 2022 17:17:26: 37000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 17:17:29: #1 tag size is determined as 51 bps INFO @ Tue, 02 Aug 2022 17:17:29: #1 tag size = 51 INFO @ Tue, 02 Aug 2022 17:17:29: #1 total tags in treatment: 42754549 INFO @ Tue, 02 Aug 2022 17:17:29: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 17:17:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 17:17:30: #1 tags after filtering in treatment: 42754505 INFO @ Tue, 02 Aug 2022 17:17:30: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 17:17:30: #1 finished! INFO @ Tue, 02 Aug 2022 17:17:30: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 17:17:30: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 17:17:32: 37000000 INFO @ Tue, 02 Aug 2022 17:17:32: 38000000 INFO @ Tue, 02 Aug 2022 17:17:33: #2 number of paired peaks: 21 WARNING @ Tue, 02 Aug 2022 17:17:33: Too few paired peaks (21) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 17:17:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX14894238/SRX14894238.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX14894238/SRX14894238.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX14894238/SRX14894238.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX14894238/SRX14894238.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 17:17:38: 39000000 INFO @ Tue, 02 Aug 2022 17:17:38: 38000000 INFO @ Tue, 02 Aug 2022 17:17:44: 40000000 INFO @ Tue, 02 Aug 2022 17:17:45: 39000000 INFO @ Tue, 02 Aug 2022 17:17:49: 41000000 INFO @ Tue, 02 Aug 2022 17:17:52: 40000000 INFO @ Tue, 02 Aug 2022 17:17:55: 42000000 INFO @ Tue, 02 Aug 2022 17:17:59: 41000000 INFO @ Tue, 02 Aug 2022 17:18:00: #1 tag size is determined as 51 bps INFO @ Tue, 02 Aug 2022 17:18:00: #1 tag size = 51 INFO @ Tue, 02 Aug 2022 17:18:00: #1 total tags in treatment: 42754549 INFO @ Tue, 02 Aug 2022 17:18:00: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 17:18:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 17:18:02: #1 tags after filtering in treatment: 42754505 INFO @ Tue, 02 Aug 2022 17:18:02: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 17:18:02: #1 finished! INFO @ Tue, 02 Aug 2022 17:18:02: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 17:18:02: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 17:18:05: #2 number of paired peaks: 21 WARNING @ Tue, 02 Aug 2022 17:18:05: Too few paired peaks (21) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 17:18:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX14894238/SRX14894238.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX14894238/SRX14894238.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX14894238/SRX14894238.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX14894238/SRX14894238.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 17:18:06: 42000000 INFO @ Tue, 02 Aug 2022 17:18:11: #1 tag size is determined as 51 bps INFO @ Tue, 02 Aug 2022 17:18:11: #1 tag size = 51 INFO @ Tue, 02 Aug 2022 17:18:11: #1 total tags in treatment: 42754549 INFO @ Tue, 02 Aug 2022 17:18:11: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 17:18:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 17:18:13: #1 tags after filtering in treatment: 42754505 INFO @ Tue, 02 Aug 2022 17:18:13: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 17:18:13: #1 finished! INFO @ Tue, 02 Aug 2022 17:18:13: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 17:18:13: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 17:18:15: #2 number of paired peaks: 21 WARNING @ Tue, 02 Aug 2022 17:18:15: Too few paired peaks (21) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 17:18:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm6/SRX14894238/SRX14894238.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX14894238/SRX14894238.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX14894238/SRX14894238.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm6/SRX14894238/SRX14894238.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。